14 results on '"Bingbing Shen"'
Search Results
2. Sesquiterpene lactones isolated from Carpesium abrotanoides L. by LC–MS combined with HSCCC inhibit liver cancer through suppression of the JAK2/STAT3 signaling pathway
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Tang Chunyu, Dan Wan, Huanghe Yu, Shuihan Zhang, Yupei Yang, Wang Xionglong, Bingbing Shen, Cai Yuan, and Daifeng Tang
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chemistry.chemical_compound ,Liquid chromatography–mass spectrometry ,Chemistry ,Jak2 stat3 ,Organic Chemistry ,medicine ,Carpesium abrotanoides ,Pharmacology ,Signal transduction ,General Pharmacology, Toxicology and Pharmaceutics ,Liver cancer ,medicine.disease ,Sesquiterpene - Abstract
Carpesium abrotanoides L. is rich in sesquiterpene lactones, the experiments of anti-tumor activity in vitro showed that total sesquiterpene lactone extracts exhibited the most cytotoxicity activities against HepG-2 cells (IC50 = 4.2 μg/mL). In order to isolated the active compounds quickly, the strategy of HPLC-ESI-Q-TOF-MS/MS combined with high-speed counter-current chromatography (HSCCC) was implemented. Three sesquiterpene lactone compounds, 2α,5α-dihydroxy-11αH-eudesma-4(15)-en-12,8β-olide (1), telekin (2), oxoeudesm-11(13)-eno-12,8α-lactone (3) were obtained. Their structures were elucidated by detailed analysis of 1D, 2D NMR and HRMS data. In addition, compounds 1-3 were evaluated for their cytotoxic activities against HepG-2 cell line with IC50 value of 9.83, 2.95, and 4.15 μM, respectively. Moreover, all compounds not only can reduce the expression of JAK2 and STAT3 mRNA but also inhibit the p-JAK2 and p-STAT3 protein expression levels in a dose‐dependent manner in the HepG-2 cells. In conclusion, three sesquiterpene lactone compounds inhibited the proliferation of HepG-2 cells via suppressing the JAK2/STAT3 signaling pathway.
- Published
- 2022
3. Virtual Sensing f-CaO Content of Cement Clinker Based on Incremental Deep Dynamic Features Extracting and Transferring Model
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Xiaoyu Jiang, Lu Xu, Jinchuan Qian, Gaopan Huang, Le Yao, Zhiqiang Ge, and Bingbing Shen
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Cement ,Computer science ,020208 electrical & electronic engineering ,Oxide ,Process (computing) ,02 engineering and technology ,computer.software_genre ,Clinker (cement) ,Data set ,chemistry.chemical_compound ,chemistry ,0202 electrical engineering, electronic engineering, information engineering ,Data mining ,Electrical and Electronic Engineering ,Distributed control system ,Instrumentation ,computer - Abstract
The content of free calcium oxide (f-CaO) in clinker significantly determines the quality of the final cement production. However, in practice, the value of f-CaO content in clinker is off-line sampled manually with a significant time interval and then analyzed in a laboratory with a large time delay, which could meet the needs for monitoring and control of cement quality. To tackle this problem, this article proposes a data-driven model based on deep dynamic features extracting and transferring methods to build a virtual sensor for f-CaO content prediction. First, in this model, large-scale unlabeled data collected from the process distributed control system (DCS) take a vital effect in extracting nonlinear dynamic features along with the limited labeled data samples. Then, the extracted features are transferred to a powerful regression model, the eXtreme Gradient Boosting (XGBoost), for output f-CaO prediction. Besides, an incremental model updating strategy is proposed for the augment of online data samples, which facilitates the virtual sensor to adapt the process time-variant characteristics. Finally, the proposed virtual sensor is verified by a data set acquired from a real cement production process. Comparing with traditional statistical modeling methods, the prediction accuracy of f-CaO content is significantly improved.
- Published
- 2021
4. Jiawei Foshou San Induces Apoptosis in Ectopic Endometrium Based on Systems Pharmacology, Molecular Docking, and Experimental Evidence
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Bingbing Shen, Yi Chen, Jiahui Wei, Chengling Zhang, Ying Zhang, Binxin Zhao, and Changxi Li
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0303 health sciences ,Article Subject ,Ectopic endometrium ,Mechanism (biology) ,Chemistry ,Poly ADP ribose polymerase ,Cell ,lcsh:Other systems of medicine ,lcsh:RZ201-999 ,Pathway analysis ,Cell biology ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,Complementary and alternative medicine ,In vivo ,Apoptosis ,030220 oncology & carcinogenesis ,medicine ,Research Article ,030304 developmental biology ,Systems pharmacology - Abstract
Foshou San is a typical gynaecological formula with wild usage in traditional Chinese medicine. Jiawei Foshou San (JFS) is a novel ingredient prescription from Foshou San with antiendometriosis effect in unclear mechanisms. To uncover the potential application and proapoptotic mechanisms of JFS, JFS ingredient-drug target-disease networks, GO enrichment, and pathway analysis were established for potential application prediction. Molecular docking and validation in vivo were investigated by the proapoptotic mechanisms of JFS. In this study, 99 common targets were related to 108 diseases. 484 biological processes, 44 cell components, 59 molecular functions, and 37 pathways were significantly identified in GO enrichment and pathway analysis. In molecular docking, ligustrazine showed binding activity with Bcl-2, Bax, caspase-9, caspase-3, and PARP. In vivo, JFS elevated the shrink rate of ectopic endometrium, by suppressing E2 and PROG. An in-depth study showed that apoptosis was activated through diminishing Bcl-2, rising Bax and Bad, and expressing more caspase-3 and caspase-9 using JFS. JFS promoted the protein level of cleaved-PARP. In brief, JFS might be applied for various diseases through multiple targets and pathways, especially endometriosis by Bcl-2 pathway. These findings reveal the potential application for further evaluation and uncover the proapoptotic mechanism of JFS.
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- 2019
5. Flavonoid glycosides from the rhizomes of Pronephrium penangianum
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Gong Limin, Bingbing Shen, Qi Zhou, Bin Liu, Yuqing Jian, Wen-Bing Sheng, Sheng-Huang Chen, Guangming Xu, Dixian Luo, Wei Wang, and Muhammad Daniyal
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0106 biological sciences ,Chalcone ,Stereochemistry ,Plant Science ,Horticulture ,01 natural sciences ,Biochemistry ,chemistry.chemical_compound ,Pigment ,Aurone ,Glycosides ,Molecular Biology ,chemistry.chemical_classification ,Flavonoids ,biology ,Molecular Structure ,010405 organic chemistry ,Glycoside ,Dihydrochalcone ,General Medicine ,biology.organism_classification ,0104 chemical sciences ,Rhizome ,chemistry ,visual_art ,visual_art.visual_art_medium ,Ferns ,Pronephrium penangianum ,Fern ,010606 plant biology & botany ,Drugs, Chinese Herbal - Abstract
Six flavonoid glycosides jixueqisus A-F, together with nine known flavonoids, were isolated from the rhizomes of the fern Pronephrium penangianum. Among them, two red pigments jixueqisus A and B possess the same rare 6,8-dimethyl-2-phenyl-7H-1-benzopyran-7-one skeleton (a long conjugated system). Jixueqisu Cwas a dihydrochalcone glycoside, jixueqisu D was a chalcone glycoside, jixueqisu E was an aurone glycoside, and jixueqisu F was a flavonone glycoside. Interestingly, jixueqisus D-F, (2S)-5,2',5'-trihydroxy-7-methoxyflavanone and 5,2',5'-trihydroxy-7-methoxyflavone possessed a 2,5-dihydroxy substituted benzene ring (B-ring). Their structures were elucidated by various spectroscopic and chemical methods. Furthermore, the plausible biosynthetic pathways of jixueqisus A-F were discussed, respectively. All isolated compounds were evaluated for their activities against the proliferation of MCF-7, HepG-2, HCT-116 and BGC-823 tumor cell lines, four known flavane-4-ol glycosides, abacopterins A and C, eruberin B and triphyllin A, exhibited moderate activities to various cell lines.
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- 2020
6. Endogenous Cys-Assisted GSH@AgNCs-rGO Nanoprobe for Real-Time Monitoring of Dynamic Change in GSH Levels Regulated by Natural Drug
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Wenming Chen, Bingbing Shen, Wen-Bing Sheng, Bin Liu, Jialong Fan, Jiantao Zhou, Wei Wang, Yupei Yang, Feng Xiao, Chunyi Tong, Qi Zhou, Muhammad Daniyal, Yan Qin, Huanghe Yu, and Wei Yang
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Silver ,Metal Nanoparticles ,Endogeny ,010402 general chemistry ,01 natural sciences ,Redox ,Analytical Chemistry ,chemistry.chemical_compound ,Limit of Detection ,Humans ,Cysteine ,Fluorescent Dyes ,chemistry.chemical_classification ,Reactive oxygen species ,Quenching (fluorescence) ,010401 analytical chemistry ,Glutathione ,Hep G2 Cells ,0104 chemical sciences ,Spectrometry, Fluorescence ,chemistry ,Biochemistry ,Apoptosis ,Doxorubicin ,Ethylmaleimide ,Graphite ,Reactive Oxygen Species ,Intracellular ,Drugs, Chinese Herbal - Abstract
Glutathione (GSH) levels are closely related to the homeostasis of redox state which directly affects human disease occurrence by regulating cell apoptosis. Hence, real-time monitoring of dynamic changes in intracellular GSH levels is urgently needed for disease early diagnosis and evaluation of therapy efficiency. In this study, an endogenous cysteine (Cys)-assisted detection system based on GSH@AgNCs and reduced graphene oxide (rGO) with high sensitivity and specificity was developed for GSH detection. Compared with GSH, GSH@AgNCs with weaker affinity and bonding force was quite easier to extrude from the rGO surface when competing against GSH, leading to the obvious change in fluorescence signal. This phenomenon was termed as "a crowding out effect". Furthermore, the presence of Cys can improve GSH assay sensitivity by enhancing the quenching efficiency of rGO on the GSH@AgNCs. In vitro assay indicated that the efficiency of fluorescence recovery was positively related with GSH concentration in the range from 0 to 10 mM. In addition, the method was employed for real-time monitoring of the dynamic changes in GSH levels regulated by natural drugs. The imaging results showed that the natural compound 3 (C3) can downregulate GSH levels in HepG2 cells, which was accompanied by reactive oxygen species (ROS) release and apoptosis induction. Finally, the method was used to monitor the change of GSH levels in serum samples with chronic hepatitis B (CHB) infection. The results demonstrated that the occurrence and development of CHB may be positively correlated with GSH levels to some extent. Overall, the above results demonstrate the potential application of this new nanosystem in anticancer natural drug screening and clinical assay regarding GSH levels.
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- 2019
7. Indole-based alkaloids from Ophiocordyceps xuefengensis
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You Qin, Shuyun Shi, Dan Wan, Bingbing Shen, Zeng Hongliang, Cheng Fei, Jun Shu, Jian Jin, Rongrong Zhou, Shui-Han Zhang, Can Zhong, and Jing Xie
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0106 biological sciences ,Stereochemistry ,Plant Science ,Horticulture ,01 natural sciences ,Biochemistry ,Indole Alkaloids ,Alkaloids ,Cell Line, Tumor ,Ic50 values ,Cytotoxicity ,Molecular Biology ,Indole test ,Molecular Structure ,010405 organic chemistry ,Cell growth ,Chemistry ,General Medicine ,Circular dichroism spectra ,0104 chemical sciences ,Hep G2 ,Ophiocordyceps xuefengensis ,Apoptosis ,A549 Cells ,Hypocreales ,010606 plant biology & botany - Abstract
Seven undescribed indole-based alkaloids, xuefengins A–D and xuefenglasins A–C, were isolated from natural Ophiocordyceps xuefengensis, along with six known alkaloids. Their structures were elucidated by comprehensive spectroscopy, with absolute configurations confirmed by comparison with calculated electronic circular dichroism spectra. Eleven of the isolates were tested for cytotoxicity against the U937, NB4, MCF-7, Hep G2, and A549 cancer cell lines. Two compounds exhibited moderate activities, with IC50 values of 2.83–25.68 μM and 1.54–12.16 μM. Further pharmacological studies showed that these two compounds inhibit cell proliferation by inducing apoptosis, and decreasing p38 and caspase-3 levels in A549 cells.
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- 2019
8. Necrostatin-1 Attenuates Renal Ischemia and Reperfusion Injury via Meditation of HIF-1α/mir-26a/TRPC6/PARP1 Signaling
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Mei Mei, Qian-Guang Pan, Youmin Pu, Hong Liu, Hongwen Zhao, Mao-Zhi Tang, Xiongfei Wu, Yue He, Huhai Zhang, and Bingbing Shen
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0301 basic medicine ,miR-26a ,Necroptosis ,HIF-1α ,TRPC6 ,necroptosis ,Inflammation ,medicine.disease_cause ,Article ,Nec-1 ,03 medical and health sciences ,0302 clinical medicine ,Drug Discovery ,medicine ,oxidative stress ,Viability assay ,Kidney ,Renal ischemia ,Chemistry ,lcsh:RM1-950 ,medicine.disease ,ischemic/reperfusion injury ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,medicine.anatomical_structure ,inflammation ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Tumor necrosis factor alpha ,medicine.symptom ,Reperfusion injury ,Oxidative stress - Abstract
Necroptosis, oxidative stress, and inflammation are major contributors to the pathogenesis of ischemic acute kidney injury. Necrostatin-1 (Nec-1), an inhibitor of the kinase domain of receptor-interacting protein kinase-1 (RIP1), has been reported to regulate renal ischemia and reperfusion (I/R) injury; however, its underlying mechanism of action remains unclear. HK-2 cells were used to create an in vitro I/R model, in which the cells were subjected to hypoxia, followed by 2, 6, and 12 h of reoxygenation. For the in vivo study, a rat model of renal I/R was established in which samples of rat blood serum and kidney tissue were harvested after reperfusion to assess renal function and detect histological changes. Cell viability and necroptosis were analyzed using the Cell Counting Kit (CCK)-8 assay and flow cytometry, respectively. The expression levels of molecules associated with necroptosis, oxidative stress, and inflammation were determined by real-time PCR, western blotting, immunofluorescence staining, and ELISA. Luciferase and chromatin immunoprecipitation (ChIP) assays were performed to confirm the relevant downstream signaling pathway. We found that pretreatment with Nec-1 significantly decreased hypoxia-inducible factor-1α (HIF-1α) and miR-26a expression, as well as the levels of factors associated with necroptosis (RIP1, RIP3, and Sirtuin-2), oxidative stress (malondialdehyde [MDA], NADP+/NADPH ratio), and inflammation (interleukin [IL]-1β, IL-10, and tumor necrosis factor alpha [TNF-α]) in I/R injury cells and the rat model. However, these effects could be reversed by miR-26a overexpression or TRPC6 knockdown. Mechanistic studies demonstrated that HIF-1α directly binds to the promoter region of miR-26a, and that TRPC6 is a potential target gene for miR-26a. Our findings indicate that Nec-1 can effectively protect against renal I/R injury by inhibiting necroptosis, oxidative stress, and inflammation, and may exert its effects through mediation of the HIF-1α/miR-26a/TRPC6/PARP1 signaling pathway. Keywords: ischemic/reperfusion injury, Nec-1, HIF-1α, miR-26a, TRPC6, necroptosis, oxidative stress, inflammation
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- 2018
9. Antimicrobial activity-guided identification of compounds from the deciduous leaves of Malus doumeri by HPLC-ESI-QTOF-MS/MS
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Bingbing Shen, Lin Chen, Rongrong Zhou, Xuejuan Liang, Luqi Huang, Shuihan Zhang, Jiayu Li, and Yupei Yang
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Active ingredient ,Malus doumeri ,Preservative ,Chromatography ,biology ,010405 organic chemistry ,Organic Chemistry ,Ethyl acetate ,Plant Science ,biology.organism_classification ,Antimicrobial ,01 natural sciences ,Biochemistry ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,chemistry ,Chlorogenic acid ,Petroleum ether ,Antibacterial activity - Abstract
This paper intends to identify the antimicrobial activity compounds from the deciduous leaves of Malus doumeri (Dong Li Tea) by HPLC-ESI-QTOF-MS/MS. The ethanol extracts of Malus doumeri were partitioned into petroleum ether, dichloromethane, ethyl acetate, n-butanol and water fraction, respectively. The antimicrobial screening experiments showed that ethyl acetate fraction has a certain antibacterial activity by inhibition zone method in vitro. And then we used the HPLC-ESI-QTOF-MS/MS method to verify the identities of bioactive compounds. Finally, 41 compounds were determined and 11 of which were firstly reported in this plant. Notably, compounds (32, 34, 38) are new dihydrochalcones, and three chlorogenic acid analogues (10, 13, 17) may be potential antimicrobial active ingredient. Which is of great significance to the isolation of novel compounds and the discovery of new natural preservative candidates from the deciduous leaves of Malus doumeri.
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- 2018
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10. A new cordycepin-producing caterpillar fungus Ophiocordyceps xuefengensis with artificial infection to the host, cultivation of mycelia and stromata
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Lin Chen, Shuihan Zhang, Dan Wan, Jian Jin, You Qin, Bingbing Shen, Cai Yuan, Lanping Zhen, Yuhui Qin, and Can Zhong
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0106 biological sciences ,0301 basic medicine ,Adenosine ,Biology ,DNA, Ribosomal ,01 natural sciences ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,010608 biotechnology ,Botany ,Genetics ,medicine ,Animals ,Caterpillar ,Molecular Biology ,Mycelium ,Deoxyadenosines ,Cordycepin ,Strain (chemistry) ,Host (biology) ,fungi ,biology.organism_classification ,Culture Media ,Lepidoptera ,030104 developmental biology ,chemistry ,Ophiocordyceps xuefengensis ,Larva ,Fermentation ,Hypocreales ,medicine.drug - Abstract
Caterpillar fungi have numerous pharmacological and therapeutic applications in traditional medicine, due to a variety of active chemical constituents, such as cordycepin and adenosine. It is imperative to discover new resource for artificial cultivation and biometabolite production since the traditional natural species are endangered. In this study, a new strain HACM 001 was isolated and identified as Ophiocordyceps xuefengensis by rDNA-ITS sequencing. This strain showed the potential of artificial infection to caterpillar larvae leading to mummification, as well as fermentation mycelia in liquid culture and cultivation stromata in solid medium. Eight nucleosides and nucleobases, especially cordycepin and adenosine, were determined and analyzed with HPLC-DAD-Q-TOF-MS/MS technology. Cordycepin was detected in all forms of present O. xuefengensis strain at different contents, among which the highest content (37.1 μg/g) appeared in the stromata cultivated on solid medium. The content of adenosine in mycelia and stromata, respectively, reached 1155 μg/g and 1470 μg/g. Therefore, O. xuefengensis might be an alternative source for obtaining artificial fungus-caterpillar-larvae complex and producing cordycepin and adenosine.
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- 2017
11. Determination and Isolation of Four Anti-tumour Saponins from Lonicera macranthoides by HPLC-ESI-QTOF/MS and HSCCC
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Dan Wan, Lin Chen, Rongrong Zhou, Luqi Huang, Shuihan Zhang, Xue-Hui Liu, Qingyijun Zhou, and Bingbing Shen
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Esi qtof ms ,Spectrometry, Mass, Electrospray Ionization ,Pharmaceutical Science ,Antineoplastic Agents ,Mass spectrometry ,01 natural sciences ,High-performance liquid chromatography ,HeLa ,03 medical and health sciences ,Anti tumour ,0302 clinical medicine ,Cell Line, Tumor ,Ic50 values ,Humans ,Chromatography, High Pressure Liquid ,Solvent system ,Chromatography ,biology ,010405 organic chemistry ,Chemistry ,Plant Extracts ,Saponins ,biology.organism_classification ,0104 chemical sciences ,Lonicera macranthoides ,Lonicera ,030220 oncology & carcinogenesis ,Biotechnology - Abstract
BACKGROUND Lonicera macranthoides is a Chinese herb that contains a large number of bioactive spanions possessing important pharmacological activities, such as anti-tumour activity. However, detailed information about their anti-tumor activity and bioactive compounds is limited. METHODS In order to evaluate the scientific basis, the method of high-speed counter-current chromatography (HSCCC) combined with high performance liquid chromatography mass spectrometry (HPLC-ESI-QTOF/MS) has been developed to separate, purify and analyze saponins from Lonicera macranthoides. Four main saponins, Macranthoidin B (I), Macranthoidin A (II), Macranthoides B (III) and Akebia saponin D (IV) were separated by HSCCC with the solvent systems of ethyl acetate-nbutanol- water (3:2:5) and n-butanol-water-methanol-ethyl acetate (1:6:0.5:4). The purities of these four bioactive ingredients (I-IV) identified and detected by HPLC-ESI-QTOF/MS were 95.1%, 92.7%, 91.8% and 96.3%, respectively. The separated saponins were evaluated for their cytotoxic activities against six tumor cell lines (MCF-7, Hela, A549, HepG2, HT29 and Eca109). RESULTS Results show that compounds I-IV exhibited particular significant anti-tumor activities against human mammary adenocarcinoma MCF-7 cell with IC50 values ranging from 12.7 to 30.8 µM. CONCLUSION It was demonstrated that the combinative method using HPLC-ESI-QTOF/MS and HSCCC was suitable for rapid screening and isolating saponins of Lonicera macranthoides and the isolated compounds have great potential for the development of new antitumor drugs.
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- 2017
12. Efficacy of N-Acetylcysteine in Preventing Acute Kidney Injury After Cardiac Surgery: A Meta-Analysis Study
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Mao-Zhi Tang, Bingbing Shen, Hong-Wen Zhao, Qian-Guang Pan, Mei Mei, and Youmin Pu
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medicine.medical_specialty ,030204 cardiovascular system & hematology ,Placebo ,Perioperative Care ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Postoperative Complications ,Medicine ,Humans ,030212 general & internal medicine ,Hospital Mortality ,Cardiac Surgical Procedures ,Adverse effect ,Creatinine ,business.industry ,Mortality rate ,Acute kidney injury ,Perioperative ,Free Radical Scavengers ,Acute Kidney Injury ,medicine.disease ,Cardiac surgery ,Acetylcysteine ,Treatment Outcome ,chemistry ,Elective Surgical Procedures ,Meta-analysis ,Anesthesia ,Surgery ,business - Abstract
Purpose: To evaluate whether perioperative N-acetylcysteine (NAC) administration reduces the risk of cardiac surgery associated acute kidney injury (CSA-AKI). Materials and Methods: A systematic literature review (Medline, PubMed, Cochrane, Biomedical central, Google Scholar) identified 10 studies (1391 patients; 695 NAC and 696 placebo) that compared the efficacy and adverse effects of perioperative NAC administration for CSA-AKI prevention in adults undergoing elective cardiac surgery. Meta-analysis was performed using Comprehensive Meta-Analysis statistical software. Results: Patients in the NAC-treated and placebo groups had similar rate of CSA-AKI occurrence, change in creatinine levels, as well as the in-hospital mortality rate (RR = 0.841, 95% CI = 0.691 to 1.023, p = 0.083; pooled difference in means = −0.328, 95% CI = −0.712 to 0.056, p = 0.094; RR = 0.741, 95% CI = 0.388 to 1.418, p = 0.366, respectively). Conclusions: Our study does not support perioperative NAC administration as a mean...
- Published
- 2017
13. Insights into Triterpene Acids in Fermented Mycelia of Edible Fungus Poria cocos by a Comparative Study
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You Qin, Jing Xie, Jian Jin, Luqi Huang, Rongrong Zhou, Can Zhong, Xuejuan Liang, Shuihan Zhang, Bingbing Shen, and Huixuan Ye
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Sclerotium ,sclerotium ,Pharmaceutical Science ,Fungus ,01 natural sciences ,Analytical Chemistry ,lcsh:QD241-441 ,Terpene ,03 medical and health sciences ,lcsh:Organic chemistry ,Triterpene ,Drug Discovery ,Food science ,Physical and Theoretical Chemistry ,HPLC-QTOF-MS/MS ,Mycelium ,030304 developmental biology ,chemistry.chemical_classification ,High concentration ,0303 health sciences ,biology ,fungi ,010401 analytical chemistry ,Organic Chemistry ,Edible fungus ,biology.organism_classification ,0104 chemical sciences ,chemistry ,Chemistry (miscellaneous) ,triterpene ,mycelium ,Molecular Medicine ,Fermentation - Abstract
As an edible sclerotia-forming fungus, Poria cocos is widely used as a food supplement and as a tonic in China. High-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (HPLC-QTOF-MS/MS) was applied to identify triterpene acids in fermented mycelia of P. cocos, as well as the epidermis and inner part of natural sclerotia. A total of 19 triterpene acids were identified in fermented mycelia, whereas 31 were identified in the epidermis and 24 in the inner part. Nine triterpene acids were quantitatively determined, and the concentrations of two valuable triterpenes, dehydropachymic acid and pachymic acid, reached 1.07 mg/g and 0.61 mg/g in the fermented mycelia part, respectively, and were both significantly higher than the concentration in the two natural parts. The fermented mycelia could be a good choice for producing some target triterpene compounds and functional foods through fermentation thanks to the high concentration of some triterpene acids.
- Published
- 2019
14. Chemical constituents of Pileostegia viburnoides var. glabrescens
- Author
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Ju-ying Zou, Bingbing Shen, Qin-wen Li, Xiang-rui Chen, Sheng-Huang Chen, Juan Yu, Wei Wang, and Min Wang
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Complementary and alternative medicine ,biology ,Chemistry ,Chemical constituents ,Botany ,Pileostegia viburnoides ,biology.organism_classification - Published
- 2014
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