1. Rational design of adjuvants for subunit vaccines: The format of cationic adjuvants affects the induction of antigen-specific antibody responses
- Author
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Daniele Veggi, Derek T. O'Hagan, Ilaria Ferlenghi, Yvonne Perrie, Signe Tandrup Schmidt, Giulia Anderluzzi, Robert Cunliffe, Stuart Woods, Barbara Baudner, and Craig W. Roberts
- Subjects
education.field_of_study ,Liposome ,Vaccines ,Chemistry ,Antigen processing ,Immunogenicity ,medicine.medical_treatment ,Cationic polymerization ,Pharmaceutical Science ,RS ,Antigen ,Biochemistry ,Adjuvants, Immunologic ,Solid lipid nanoparticle ,Antibody Formation ,Liposomes ,Vaccines, Subunit ,medicine ,Tissue Distribution ,Dimethyldioctadecylammonium bromide ,Antigens ,education ,Adjuvant - Abstract
A range of cationic delivery systems have been investigated as vaccine adjuvants, though few direct comparisons exist. To investigate the impact of the delivery platform, we prepared four cationic systems (emulsions, liposomes, polymeric nanoparticles and solid lipid nanoparticles) all containing equal concentrations of the cationic lipid dimethyldioctadecylammonium bromide in combination with the Neisseria adhesin A variant 3 subunit antigen. The formulations were physicochemically characterized and their ability to associate with cells and promote antigen processing (based on degradation of DQ-OVA, a substrate for proteases which upon hydrolysis is fluorescent) was compared in vitro and their vaccine efficacy (antigen-specific antibody responses and IFN-γ production) and biodistribution (antigen and adjuvant) were evaluated in vivo. Due to their cationic nature, all delivery systems gave high antigen loading (> 85%) with liposomes, lipid nanoparticles and emulsions being
- Published
- 2020