1. Stability studies of the components of a prototype penicillinase (beta-lactamase)-linked ELISA kit
- Author
-
M. I. Khatkhatay, U. M. Joshi, D. K. Pardhe, G. M. Sankolli, and Meena Desai
- Subjects
Microbiology (medical) ,Chromatography ,medicine.diagnostic_test ,Chemistry ,medicine.medical_treatment ,Biochemistry (medical) ,Clinical Biochemistry ,Reproductive hormones ,Public Health, Environmental and Occupational Health ,Substrate (chemistry) ,Enzyme-Linked Immunosorbent Assay ,Hematology ,Immunosorbents ,Penicillinase ,Medical Laboratory Technology ,Elisa kit ,Immunoassay ,Enzyme Stability ,medicine ,Beta-lactamase ,Immunology and Allergy ,Reagent Kits, Diagnostic ,After treatment ,Conjugate - Abstract
Penicillinase (beta-lactamase) enzyme-linked immunosorbent assay (ELISA) for various reproductive hormones developed in the laboratory were found to have wide applicability in the fertility check clinic of the Institute. A need was thought to transform these assays into ready-to-use kit forms. Therefore, prototype ELISA kits for these hormones were developed and stability of the individual component was ascertained at various temperatures (room temperature, 37 degrees C and 2-8 degrees C). Stability studies were conducted on previously validated assay for pregnanediol-3 alpha-glucuronide (PdG). The studies showed that immunosorbents (antibody coated plates) are stable at room temperature for a period of 2 weeks, at 37 degrees C for 1 week and at 2-8 degrees C for a period of 9 months when preserved after treatment with glycerol solution. The lyophilised conjugate, standard and immunoassay buffer, colour reagent, and its substrate were stable at 37 degrees C up to 1 week and at room temperature up to 2 weeks and at 2-8 degrees C for a period of 6 months, during which the stability was studied.
- Published
- 1993