677 results on '"Dong, Yan"'
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2. Nonlinear Robust Adaptive Control of Universal Manipulators Based on Desired Trajectory
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Yu Chen, Jianwan Ding, and Dong Yan
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trajectory tracking control ,nonlinear robust control ,adaptive control ,Lyapunov stability ,desired trajectory ,manipulator ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
The introduction of a dynamic model in robot trajectory tracking control design can significantly improve its trajectory tracking accuracy, but there are many uncertainties in the robot dynamic model which can be dealt with through robust control and adaptive control. The prevailing robust control as well as adaptive control methods require real-time computation of robot dynamics, but the extreme complexity of the robot dynamics equations makes it difficult to apply these methods in real industrial systems. To this end, this article proposes a robust adaptive control method based on the desired trajectory, which uses the desired trajectory to compute most of the control terms offline, including the robot’s nominal dynamics and regression matrices, and substantially reduces the need for real-time computation of the feedback signals. The robust term modifies the perturbation of the inertial parameters of the links, the adaptive term learns the friction coefficients of the joints online, and an additional compensation term is designed to satisfy the Lyapunov stability condition of the system. Finally, taking a universal manipulator as the experimental platform, the control performances of different control methods are compared to show the feasibility of the controller and the effective reduction in real-time computational complexity.
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- 2024
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3. Experimental Study on the Effects of Heavy Metal Pollution on Soil Physical Properties and Microstructure Evolution
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Guoliang Zhang, Tao Liu, Haifeng Li, Zimou Wang, Xixi Huang, Xiangyang Yi, and Dong Yan
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heavy metal pollution ,shear strength ,resistivity ,microstructure ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Soil heavy metal pollution poses a formidable challenge for environmental protection professionals. This study delves into the impact of zinc and lead pollution on the particle size distribution, liquid-plastic limit, permeability, shear strength, and other pertinent physical and engineering properties of clay. The alterations in the microstructure of soil contaminated by heavy metals were scrutinized using a scanning electron microscope. The findings reveal that as the concentration of heavy metals in contaminated soil rises, there is a concurrent decrease in the liquid limit, plasticity index, and silt content. This, in turn, leads to the deterioration of the original fluidity and plasticity of the soil, accompanied by a reduction in fine particles. Resistivity tests indicate that an escalation in water content results in a decrease in resistivity, an increase in porosity leads to an increase in resistivity, and an elevation in the concentration of heavy metals precipitates a sharp decline in resistivity due to the heightened conductivity of heavy metal ions. Heavy metal pollution induces structural changes in the soil, particularly in pore size, thereby influencing the permeability coefficient.
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- 2024
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4. Water Consolidation Performance of Acrylic-Polymer-Modified Materials and Their Concrete Impermeability Repair Characteristics
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Dong Yan, Lipeng Lai, Xuedang Xiao, Lei Zhang, Zilong Zhao, and Jun Zhao
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grouting material ,acrylate ,polymer modification ,impermeability repair ,permeability coefficient ,Science ,Chemistry ,QD1-999 ,Inorganic chemistry ,QD146-197 ,General. Including alchemy ,QD1-65 - Abstract
Acrylic materials exhibit favorable grouting repair performance. However, their curing products are easily inclined to drying shrinkage, and their concrete impermeability repair characteristics have seldom been investigated. To improve material properties, reveal the impermeability repair mechanism, and address drying shrinkage, this study proposed the addition of styrene–acrylate copolymer emulsion (styrene–acrylic emulsion) to the grouting material to prepare two-component acrylate grouting materials. Using orthogonal and single-factor tests combined with physical and mechanical properties, the mechanical properties and impermeability repair performance (physical and mechanical properties combined) of grouting materials were analyzed and studied, and the optimal ratio of each component of acrylate grouting materials was determined. Results show that (1) the hydrogel produced by the reaction of sodium methacrylate with hydroxyethyl acrylate has good physical and mechanical properties. (2) With the increase in the accelerator dosage, the setting time of slurry initially decreases and then increases; as the initiator dosage increases, the setting time of slurry decreases, which is negatively correlated with the initiator dosage. (3) Talcum powder can improve the physical and chemical properties of gel and enhance the reliability and durability of acrylate grouting materials, and the comprehensive performance is the best at a dosage of 3%. (4) Styrene–acrylic emulsion can increase the solid content and reduce the volume drying shrinkage when added to grouting materials. The fractured impermeable specimens were repaired by grouting with prepared acrylate grouting materials and cured for 24 h for the impermeability test, and the water pressure for the 24 h impermeability repair was 1.0 MPa. This study’s results provide important reference and basis for revealing the impermeability principle of acrylate grouting materials and evaluating their impermeability.
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- 2023
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5. Experimental Study on the Effect of the Freeze–Thaw Cycle in an Acidic Environment on the Mechanical Properties of Grout-Reinforced Rock Specimens
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Dong Yan, Jianguo Zheng, Xixi Huang, and Tao Liu
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grouting reinforcement ,chemical corrosion ,freeze–thaw cycle ,mechanical properties ,microscopic analysis ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
A new type of grouting material—FCM (fast cementing material)—is being used in coastal and offshore infrastructure projects, such as harbor and tunnel rehabilitation. In order to investigate how this material performs under different conditions, the compressive strength, failure mode, and surface microscopic changes of different fracture penetration degrees and different crack angles of grout-reinforced specimens formed by the FCM and sandstone were investigated in an acidic environment with pH = 1.1 and after freeze–thaw cycles of 5, 10, 20, and 30 times. In addition, through the preliminary determination of FCM grouting material fluidity and setting time, it was determined that the water-material ratio in this test is 0.3, and the sandstone used has good uniformity. The results show that the strength of the original rock can be matched or even exceeded by the solid grout of FCM and sandstone. Acidic environments and freeze–thaw cycles will erode the specimens, mainly on the surface of the specimens. The fracture penetration degree and crack angle determine the degree of strength reduction of the specimens. The compressive strength of the specimens decreases most rapidly within 10 freeze–thaw cycles. After soaking in acidic solution and a freeze–thaw cycle in an acidic environment, particle shedding occurs on the surface of the grout material and a “honeycomb” area appears on the surface of the sandstone. As the freeze–thaw cycle increases, the particle-shedding area and the honeycomb area increase continuously.
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- 2023
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6. Discrete Element-Based Simulation Analysis and Research of Potato Soil Agglomerate Fragmentation and Separation
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Dong Yan, Weigang Deng, Shengshi Xie, Chenglong Liu, Zhiqi Ren, Haohao Zhao, Yansong Cai, and Zexin Zhao
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overburden rotating plate ,EDEM ,discrete element ,potato soil agglomerates ,fragmentation and separation ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
To study the influence law of the overburden rotating plate mechanism on the fragmentation and separation of potato soil agglomerates, a single-factor test and a response surface test simulation analysis of the soil fragmentation process were conducted in EDEM 2022 software. The results of the single-factor test show that the triangular rack blade of the overburdened rotating plate mechanism has the best effect on soil fragmentation and separation. With the increase in the lower blade speed, the upper lift chain bar line speed, and the tilt angle of the mechanism, the effect on the fragmentation and separation of potato soil agglomerates decreases. The response surface test results show that the debris removal rate decreases with the increase in blade speed and tilt angle, the percentage of bond breakage between potato soil particles declines with the rise of blade speed and lift chain bar line speed, and the percentage of bond breakage between soil particles increases with the increase in blade speed and lift chain bar line speed. The optimal solution was obtained by using the optimization function in Design-Expert 13 software, which was adjusted as follows: the blade rack type was triangular, the lift chain bar line speed was 0.307 m/s, the blade speed was 0.4 m/s, and the tilt angle was 40°. The research methods and results provide a reference for the simulation of potato soil crushing and separation motion in a sandy loam soil cultivation area.
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- 2023
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7. Multimode-ultrasound and microwave assisted natural ternary deep eutectic solvent sequential pretreatments for corn straw biomass deconstruction under mild conditions
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Dong Yan, Qinghua Ji, Xiaojie Yu, Mo Li, Olugbenga Abiola Fakayode, Abu ElGasim A. Yagoub, Li Chen, and Cunshan Zhou
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Corn straw ,Lignocellulosic biomass ,Ultrasound-microwave ,Green solvent ,Mild conditions ,Cellulose ,Chemistry ,QD1-999 ,Acoustics. Sound ,QC221-246 - Abstract
Mild and effective pretreatments are essential to deconstruct lignocellulosic biomass so as to reuse cellulose content for value-added products. In this study, sequential multimode-ultrasound and microwave with natural ternary deep eutectic solvent (NATDES) pretreatments were used to deconstruct corn straw and optimized factors such as NATDES, ultrasonic, and microwave parameters. Results indicated that the ultrasound-NATDES or microwave-NATDES pretreatment could remove 37.86% and 52.36% lignin, respectively. When using sequential multimode-ultrasound and microwave assisted NATDES pretreatment, the delignification efficiency increased to 61.50%, and the cellulose content increased from 34.70% to 76.08%. In addition, the delignification of sequential multimode-ultrasound and microwave assisted NATDES pretreatment (under the mild conditions of microwave heating at 60 °C and 60 min) increased to 57.39%, and the cellulose content increased to 59.98%, too. This highlighted the effect of the combined ultrasound and microwave technology. Finally, the microstructural changes of mercury intrusion porosimeters, scanning electron microscopy, thermogravimetric, X-ray diffraction and Fourier transform mid-infrared spectroscopy were conducted to confirm the effectiveness of this method to deconstruct corn straw. A mechanism of the deconstruction of corn straw biomass in NATDES with the assistance of the sequential multimode-ultrasound and microwave heating was proposed. This research could open a window for future use of biomass energy by deconstructing lignocellulosic biomasses using environmentally friendly pretreatment methods.
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- 2021
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8. Conjunctive Analyses of BSA-Seq and BSR-Seq Unveil the Msβ-GAL and MsJMT as Key Candidate Genes for Cytoplasmic Male Sterility in Alfalfa (Medicago sativa L.)
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Le Zhou, Yingzhe Wang, Xiaobo Xu, Dong Yan, Weijie Yu, Yifan Miao, and Bo Xu
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Medicago sativa L. ,cytoplasmic male sterility ,BSA-seq ,BSR-seq ,DEGs ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Knowing the molecular mechanism of male sterility in alfalfa is important to utilize the heterosis more effectively. However, the molecular mechanisms of male sterility in alfalfa are still unclear. In this study, the bulked segregant analysis (BSA) and bulked segregant RNA-seq (BSR) were performed with F2 separation progeny to study the molecular mechanism of male sterility in alfalfa. The BSA-seq analysis was located in a candidate region on chromosome 5 containing 626 candidate genes which were associated with male sterility in alfalfa, while the BSR-seq analysis filtered seven candidate DEGs related to male sterility, and these candidate genes including EF-Tu, β-GAL, CESA, PHGDH, and JMT. The conjunctive analyses of BSR and BSA methods revealed that the genes of Msβ-GAL and MsJMT are the common detected candidate genes involved in male sterility in alfalfa. Our research provides a theory basis for further study of the molecular mechanism of male sterility in alfalfa and significant information for the genetic breeding of Medicago sativa.
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- 2022
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9. High performance and stable mesoporous MgO–ZrO2 supported Ni catalysts for dry reforming of methane
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Xinghua Huang, Yijun Ji, Tong Wei, Lichao Jia, Dong Yan, and Jian Li
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Dry reforming of methane ,Catalyst ,Mesoporous MgO–ZrO2 ,Carbon deposition ,Stability ,Chemistry ,QD1-999 - Abstract
Catalysts for dry reforming of methane (DRM) based on Ni are used widely. However, due to carbon deposition or active metal sintering, deactivation is inevitably attached to these catalysts. Ni catalysts supported on mesoporous MgO–ZrO2 have been synthesized by surfactant-assisted route and impregnation method, and their catalytic performance for DRM reaction has been investigated in this study. The specific surface area of the mesoporous support is approximately 64.7 m2 g−1 after calcination at 700° for 2 h. Catalyst with 10 wt% Ni content shows the best catalytic performance. The conversion rates of CH4 and CO2 are higher than 80%, while the selectivity values of CO and H2 are higher than 90%. After a long-term DRM test, according to the weight loss by TG, the catalysts carbon deposition with 7.5 wt% and 10 wt% content are 46.2% and 19.9%, respectively.
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- 2021
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10. Biomolecule@COF: Natural-artificial hybrid microcapsules for controllable biocatalysis
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Sainan Zhang, Dong Yan, Yao Chen, Jiangyue Yu, Shan Qiao, Zhenjie Zhang, and Mingmin Li
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chemistry.chemical_classification ,Materials science ,General Chemical Engineering ,Biomolecule ,Composite number ,02 engineering and technology ,021001 nanoscience & nanotechnology ,020401 chemical engineering ,Chemical engineering ,chemistry ,Biocatalysis ,Mass transfer ,PEG ratio ,General Materials Science ,0204 chemical engineering ,0210 nano-technology - Abstract
The assembly of biomacromole particles with artificial solid matrixes to fabricate hybrid composites has been demonstrated as an efficient strategy to promote both components’ applications. Elaborating matrixes as well as appropriate assembly method is essential for optimal performances of the obtained natural-artificial composites. Herein, we fabricated the Cyt c@COF-42-B microcapsule through a sacrificial templating method and researched their adjustable biocatalysis by precisely adjusting the microenvironments of these assembled enzyme@COF particles. As a result, the hydrophobicity and mass transfer of the COF microcapsule can be controlled by modifying the different amounts of PEG moieties, thus improving the composite's bioactivity. We found that compared with pristine Cyt c@COF-42-B without PEG, the Cyt c@COF-42-B-PEG exhibited a ∼2.2 times higher activity. This study provides a significant step toward designing customizable functional natural-artificial composites for enhanced biomacromolecule's activity.
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- 2022
11. Impacts of changes in intestinal flora on the metabolism of Sprague–Dawley rats
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Dong Yan, Jianrong Huang, Yongtao Li, and Chengfei Wang
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Male ,Serum ,Salmonella ,medicine.medical_specialty ,Salmonella enteritidis ,Bioengineering ,Ileum ,Biology ,medicine.disease_cause ,Applied Microbiology and Biotechnology ,Gas Chromatography-Mass Spectrometry ,Butyric acid ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Feces ,Intestinal flora ,Internal medicine ,medicine ,gc/ms ,Animals ,salmonella enteritidis ,real-time pcr ,Alanine ,Principal Component Analysis ,General Medicine ,Metabolism ,metabolomics ,Gastrointestinal Microbiome ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Metabolome ,Leucine ,Isoleucine ,TP248.13-248.65 ,Biotechnology ,Research Article ,Research Paper - Abstract
Changes in intestinal flora affect the health and cause metabolic diseases of the host. The extent to which the impact of different changes in intestinal flora would have on the metabolism of an individual has not been reported. This study aims to investigate the effect of different changes in intestinal flora on the metabolism of Sprague–Dawley (SD) normal rats’ individuals. Forty-eight SD rats were randomly divided into 6 groups (8 rats per group), which were treated with normal saline, probiotics, nonpathogenic Escherichia coli, Salmonella enteritidis, gentamicin, and magnesium sulfate, respectively. After 7 days, the ileum of each group of rats was collected and real-time polymerase chain reaction was used to analyze the composition of intestinal flora. And gas chromatography/mass spectrometry (GC/MS) was used to analyze plasma metabolic profile. The results revealed that the decrease in alanine content in the probiotics group was statistically significant, while the alanine content in the nonpathogenic Escherichia group increased significantly. Alanine, leucine, isoleucine, and serine decreased significantly in the Salmonella group. Proline and butyric acid decreased significantly in the gentamicin group. The principal component analysis showed significant differences in the Salmonella group compared with other test groups. Overall, the most significant metabolic changes were observed in SD rats in the Salmonella group, while a great similarity was observed in the probiotics, Escherichia group, and gentamicin groups compared with the normal group. Changes in intestinal flora had a certain impact on the metabolism in SD rats, especially on amino acid levels.
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- 2021
12. An Exfoliation–Evaporation Strategy To Regulate N Coordination Number of Co Single-Atom Catalysts for High-Performance Lithium–Sulfur Batteries
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Yew Von Lim, Dong Yan, Shaozhuan Huang, Daliang Fang, Jenh-Yih Juang, Pan Sun, Bing-Jian Su, Yang Shang, Hui Ying Yang, Xue Liang Li, and Ching-Yuan Su
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inorganic chemicals ,Chemistry ,General Chemical Engineering ,Coordination number ,Kinetics ,Biomedical Engineering ,Evaporation (deposition) ,Exfoliation joint ,Redox ,Catalysis ,Chemical engineering ,embryonic structures ,Atom ,Moiety ,General Materials Science - Abstract
Single-atom catalysts (SACs) with metal–nitrogen (M–N) moiety are effective in boosting the redox kinetics of lithium–sulfur (Li–S) batteries. However, the precise preparation of SACs with controll...
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- 2021
13. Interplay between the Gut Microbiome and Metabolism in Ulcerative Colitis Mice Treated with the Dietary Ingredient Phloretin
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Dong Yan, Puze Li, Genshen Zhong, Jinsong Qi, Min Li, Jie Ren, Minna Wu, and Mingyong Wang
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Male ,Phloretin ,Metabolite ,Flavonoid ,Gut flora ,Pharmacology ,Applied Microbiology and Biotechnology ,Feces ,Mice ,chemistry.chemical_compound ,Mesalazine ,RNA, Ribosomal, 16S ,medicine ,Animals ,chemistry.chemical_classification ,biology ,digestive, oral, and skin physiology ,General Medicine ,Metabolism ,biology.organism_classification ,Gastrointestinal Microbiome ,Mice, Inbred C57BL ,Metabolic pathway ,chemistry ,Colitis, Ulcerative ,Netilmicin ,Metabolic Networks and Pathways ,Biotechnology ,medicine.drug - Abstract
A growing number of healthy dietary ingredients in fruits and vegetables have been shown to exhibit diverse biological activities. Phloretin, a dihydrochalcone flavonoid that is abundant in apples and pears, has anti-inflammatory effects on ulcerative colitis (UC) mice. The gut microbiota and metabolism are closely related to each other due to the existence of the food-gut axis in the human colon. To investigate the interplay of faecal metabolites and the microbiota in UC mice after phloretin treatment, phloretin (60 mg/kg) was administered by gavage to ameliorate dextran sulfate sodium (DSS)-induced UC in mice. Gut microbes and faecal metabolite profiles were detected by high-throughput sequencing and liquid chromatography mass spectrometry (LC-MS) analysis, respectively. The correlations between gut microbes and their metabolites were evaluated by Spearman correlation coefficients. The results indicated that phloretin reshaped the disturbed faecal metabolite profile in UC mice and improved the metabolic pathways by balancing the composition of faecal metabolites such as norepinephrine, mesalazine, tyrosine, 5-acetyl-2,4-dimethyloxazole, and 6-acetyl-2,3-dihydro-2-(hydroxymethyl)-4(1H)-pyridinone. Correlation analysis identified the relations between the gut microbes and their metabolites. Proteus was negatively related to many faecal metabolites, such as norepinephrine, L-tyrosine, laccarin, dopamine glucuronide, and 5-acetyl-2,4-dimethyloxazole. The abundance of unidentified Bacteriodales_S24-7_group was positively related to ecgonine, 15-KETE and 6-acetyl-2,3-dihydro-2-(hydroxymethyl)-4(1H)-pyridinone. The abundance of Christensenellaceae_R-7_group was negatively related to the levels of 15-KETE and netilmicin. Stenotrophomonas and 15-KETE were negatively related, while Intestinimonas and alanyl-serine were positively related. In conclusion, phloretin treatment had positive impacts on faecal metabolites in UC mice, and the changes in faecal metabolites were closely related to the gut microbiota.
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- 2021
14. Metabolomics Analysis of the Effect of GAT-2 Deficiency on Th1 Cells in Mice
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Guoqiang Zhu, Xiaojie Zhang, Baobao Liu, Dong Yan, and Xueyan Ding
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GABA Plasma Membrane Transport Proteins ,genetic structures ,Polymers ,Chemistry ,Cellular differentiation ,Transporter ,General Chemistry ,Th1 Cells ,Membrane transport ,Biochemistry ,Gas Chromatography-Mass Spectrometry ,eye diseases ,Mice ,Metabolic pathway ,Metabolomics ,Immune system ,Animals ,Purine metabolism ,Cysteine - Abstract
The classic neurotransmitter γ-aminobutyric acid (GABA) has been shown to shape the activation and function of immune cells. There are four high-affinity GABA transporters (GATs, including GAT-1, GAT-2, GAT-3, and GAT-4) responsible for the transmembrane transport of GABA in mice. To explore the effect of GAT-2 on type 1 helper T (Th1) cells, naive CD4+ T cells were isolated from splenocytes of GAT-2 knockout (KO) and wild-type (WT) mice and cultured for Th1 cell differentiation, and then, metabolomics analysis of Th1 cells was performed via gas chromatography coupled to time-of-flight mass spectrometry added with multivariate analyses. Based on the variable importance projection value > 1 and P < 0.05, a total of nine differentially expressed metabolites (DEMs) were identified between WT and KO. Then, DEMs were mapped to the KEGG database, and five metabolic pathways were significantly enriched, including the cysteine and methionine metabolism, the riboflavin metabolism, the purine metabolism, the glycerolipid metabolism, and the glycerophospholipid metabolism. Collectively, our metabolomics analysis revealed that deficiency of GAT-2 influenced the metabolomics profile of Th1 cells, which will provide insights into T cell response to GAT-2 deficiency in mice. Data are available via MetaboLights with identifier MTBLS3358.
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- 2021
15. Prompting structure stability of O3–NaNi0.5Mn0.5O2 via effective surface regulation based on atomic layer deposition
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Dong Yan, Shuwei Sun, Kai Du, Huiling Zhao, Caiyan Yu, Ying Bai, Hui Ying Yang, and Linying Yang
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Materials science ,Oxide ,Nanotechnology ,02 engineering and technology ,engineering.material ,Surface engineering ,01 natural sciences ,law.invention ,chemistry.chemical_compound ,Atomic layer deposition ,Coating ,law ,0103 physical sciences ,Materials Chemistry ,Dissolution ,010302 applied physics ,Process Chemistry and Technology ,021001 nanoscience & nanotechnology ,Cathode ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry ,Ceramics and Composites ,engineering ,Degradation (geology) ,0210 nano-technology ,Layer (electronics) - Abstract
Layered O3 type oxides exhibit promising prospects as high-performance cathodes for sodium-ion batteries (SIBs) due to their low cost and high theoretical capacities. Nevertheless, the intrinsic surface composition and bulk structure degradation upon cycling presents a huge obstacle to stable sodium-ion storage/transportation. Besides, the effective surface decoration on layered O3 oxides is still challenging through conventional wet chemical route owing to their extraordinarily high surface sensitivities. Herein, a typical O3 type layered oxide of NaNi0.5Mn0.5O2 (NNMO) was selected and successfully encapsulated by precisely controlled Al2O3 layers via atomic layer deposition (ALD) technology. With the optimally controlled Al2O3 thickness of 3 nm, the surface regulated NNMO delivers a highly reversible capacity of 73.6 mA h g-1, with a significantly improved capacity retention of 68.0% after 300 cycles at 0.5 C, and a superior rate capability of 65.8 mA h g-1 at 10 C. Further air sensitivity tests demonstrate that the protective layer could effectively mitigate the generation of sodium-based impurities on NNMO, and reduce the surface sensitivities. Both chemical and electrochemical aging tests confirm the contribution of Al2O3 coating layer in alleviating ion dissolution as well as stabilizing the structure and morphology of NNMO. Based on regulating the surface of O3 type layered oxides utilizing ALD technique, this work supplies an effective and facile strategy to overcome the challenges from fast structure degradation and electrochemical property decay, which not only highlights the significance and effectiveness of surface engineering in secondary batteries, but also sheds light on accurate interface construction and regulation for active electrode materials, particularly for those sensitive to ambient atmosphere.
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- 2021
16. Severe fever with thrombocytopenia syndrome virus (SFTSV)-host interactome screen identifies viral nucleoprotein-associated host factors as potential antiviral targets
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Peng Luo, Kenn Ka-Heng Chik, Ronghui Liang, Zi-Wei Ye, Kwok-Yung Yuen, Kaiming Tang, Feifei Yin, Shuofeng Yuan, Lei Wen, Chris Chun-Yiu Chan, Gang Lu, Jasper Fuk-Woo Chan, Zhenzhi Qin, Hin Chu, Yaoqiang Huang, Jiang Du, Dong-Yan Jin, and Jianli Cao
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Artenimol ,Interactome ,Biophysics ,Druggability ,Pathogenesis ,Biology ,Biochemistry ,Virus ,chemistry.chemical_compound ,Structural Biology ,Omacetaxine mepesuccinate ,Genetics ,Host factor ,ComputingMethodologies_COMPUTERGRAPHICS ,Bunyavirales ,Virology ,Computer Science Applications ,Nucleoprotein ,Treatment ,chemistry ,SFTSV ,DrugBank ,TP248.13-248.65 ,Severe fever with thrombocytopenia syndrome virus ,Biotechnology ,Research Article - Abstract
Graphical abstract, Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne virus that causes severe infection in humans characterized by an acute febrile illness with thrombocytopenia and hemorrhagic complications, and a mortality rate of up to 30%. Understanding on virus-host protein interactions may facilitate the identification of druggable antiviral targets. Herein, we utilized liquid chromatography-tandem mass spectrometry to characterize the SFTSV interactome in human embryonic kidney-derived permanent culture (HEK-293T) cells. We identified 445 host proteins that co-precipitated with the viral glycoprotein N, glycoprotein C, nucleoprotein, or nonstructural protein. A network of SFTSV-host protein interactions based on reduced viral fitness affected upon host factor down-regulation was then generated. Screening of the DrugBank database revealed numerous drug compounds that inhibited the prioritized host factors in this SFTSV interactome. Among these drug compounds, the clinically approved artenimol (an antimalarial) and omacetaxine mepesuccinate (a cephalotaxine) were found to exhibit anti-SFTSV activity in vitro. The higher selectivity of artenimol (71.83) than omacetaxine mepesuccinate (8.00) highlights artenimol’s potential for further antiviral development. Mechanistic evaluation showed that artenimol interfered with the interaction between the SFTSV nucleoprotein and the host glucose-6-phosphate isomerase (GPI), and that omacetaxine mepesuccinate interfered with the interaction between the viral nucleoprotein with the host ribosomal protein L3 (RPL3). In summary, the novel interactomic data in this study revealed the virus-host protein interactions in SFTSV infection and facilitated the discovery of potential anti-SFTSV treatments.
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- 2021
17. Scoparone inhibits pancreatic cancer through PI3K/Akt signaling pathway
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Fan Yang, Nan Ge, Dong-Yan Liu, Jintao Guo, Siyu Sun, and Na Li
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Akt1 ,business.industry ,Akt/PKB signaling pathway ,Gastroenterology ,AKT1 ,Pancreatic cancer ,Xenograft tumor ,Basic Study ,Cell cycle ,medicine.disease ,Scoparone ,chemistry.chemical_compound ,Bioinformatics analysis ,Oncology ,chemistry ,Apoptosis ,PI3K/Akt signaling pathway ,Cancer research ,Medicine ,business ,Protein kinase B ,PI3K/AKT/mTOR pathway - Abstract
Background Pancreatic cancer is a highly malignant tumor of the gastrointestinal system whose emerging resistance to chemotherapy has necessitated the development of novel antitumor treatments. Scoparone, a traditional Chinese medicine monomer with a wide range of pharmacological properties, has attracted considerable attention for its antitumor activity. Aim To explore the potential antitumor effect of scoparone on pancreatic cancer and the possible molecular mechanism of action. Methods The target genes of scoparone were determined using both the bioinformatics and multiplatform analyses. The effect of scoparone on pancreatic cancer cell proliferation, migration, invasion, cell cycle, and apoptosis was detected in vitro. The expression of hub genes was tested using quantitative reverse transcription polymerase chain reaction (qRT-PCR), and the molecular mechanism was analyzed using Western blot. The in vivo effect of scoparone on pancreatic cancer cell proliferation was detected using a xenograft tumor model in nude mice as well as immunohistochemistry. Results The hub genes involved in the suppression of pancreatic cancer by scoparone were obtained by network bioinformatics analyses using publicly available databases and platforms, including SwissTargetPrediction, STITCH, GeneCards, CTD, STRING, WebGestalt, Cytoscape, and Gepia; AKT1 was confirmed using qRT-PCR to be the hub gene. Cell Counting Kit-8 assay revealed that the viability of Capan-2 and SW1990 cells was significantly reduced by scoparone treatment exhibiting IC50 values of 225.2 μmol/L and 209.1 μmol/L, respectively. Wound healing and transwell assays showed that scoparone inhibited the migration and invasion of pancreatic cancer cells. Additionally, flow cytometry confirmed that scoparone caused cell cycle arrest and induced apoptosis. Scoparone also increased the expression levels of Bax and cleaved caspase-3, decreased the levels of MMP9 and Bcl-2, and suppressed the phosphorylation of Akt without affecting total PI3K and Akt. Moreover, compared with the control group, xenograft tumors, in the 200 μmol/L scoparone treatment group, were smaller in volume and lighter in weight, and the percentages of Ki65- and PCNA-positive cells were decreased. Conclusion Our findings indicate that scoparone inhibits pancreatic cancer cell proliferation in vitro and in vivo, inhibits migration and invasion, and induces cycle arrest and apoptosis in vitro through the PI3K/Akt signaling pathway.
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- 2021
18. Butanol Promoting High Graphitization in Carbon‐Supported Na 3 V 2 (PO 4 ) 3 for High‐Power Sodium‐Ion Battery with Long Life Cycle
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Shuhao Xiao, Zhenzhe Li, Dong Yan, Jun Song Chen, Xinyan Li, Jianyang Jiang, and Rui Wu
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chemistry.chemical_compound ,Materials science ,chemistry ,Chemical engineering ,Butanol ,Electrochemistry ,chemistry.chemical_element ,Sodium-ion battery ,Carbon ,Catalysis ,Power (physics) - Published
- 2021
19. <scp>Trilobolide‐6‐O</scp> ‐isobutyrate suppresses hepatocellular carcinoma tumorigenesis through inhibition of <scp>IL</scp> ‐6/ <scp>STAT3</scp> signaling pathway
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Jin Shang, Rui-Han Li, Rui Fan, Dong-Yan Shen, Yang Hui, Hui-Qi Li, Xiu-Qiao Zhou, Zhan-Xiang Wang, Tong Zhang, Wen-Hao Chen, Si-Yang Li, and Xiao-Mei Mao
- Subjects
STAT3 Transcription Factor ,Carcinoma, Hepatocellular ,Cyclin E ,Cell cycle checkpoint ,Carcinogenesis ,Apoptosis ,medicine.disease_cause ,Stat3 Signaling Pathway ,Isobutyrates ,Cell Line, Tumor ,medicine ,Humans ,Furans ,Cyclin B1 ,STAT3 ,Cell Proliferation ,Pharmacology ,biology ,Interleukin-6 ,Chemistry ,Liver Neoplasms ,Butyrates ,Cancer research ,biology.protein ,Signal transduction ,Signal Transduction - Abstract
Currently available therapies for hepatocellular carcinoma (HCC), with a high morbidity and high mortality, are only marginally effective and with sharp adverse side effects, which makes it compulsory to explore novel and more effective anticancer molecules. Chinese medicinal herbs exhibited prominent anticancer effects and were applied to supplement clinical cancer treatment. Here, we reported a compound, trilobolide-6-O-isobutyrate (TBB), isolated from the flowers of Wedelia trilobata with a markedly cytotoxic effect on HCC cells. We found that TBB time- and dose-dependently inhibited HCC cells' growth and colony formation in vitro. Moreover, TBB induced cell cycle arrest at the G2/M phase, mitochondrial caspase-dependent apoptosis, and suppressed migration and invasion, as well as the glycolysis of HCC cells. Mechanistically, our data indicated that TBB inhibited the STAT3 pathway activation by directly interacting with the TYR 640/657 sites of the STAT3 protein and decreasing the level of p-STAT3. TBB also regulated the expression of PCNA, Ki67, Cyclin B1, Cyclin E, Bax, Bcl2, MMP2/9, and PGK1 through the inhibition of the IL-6/STAT3 signaling pathway. Lastly, we confirmed that TBB effectively eliminated tumor growth without causing overt toxicity to healthy tissues in the xenograft tumor model. The exploration of anticancer activity and the underlying mechanism of TBB suggested its usage as a promising chemotherapeutic agent for HCC.
- Published
- 2021
20. Effect of berbamine on invasion and metastasis of human liver cancer SMMC-7721 cells and its possible mechanism
- Author
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Ying Caoa, Jian-bo Cao, Li-li Liu, Binbin Yu, and Jia-dong Yan
- Subjects
Cancer Research ,Berbamine ,Benzylisoquinolines ,Connexins ,Cx32 ,Metastasis ,chemistry.chemical_compound ,medicine ,Humans ,Gene silencing ,Neoplasm Invasiveness ,Pharmacology (medical) ,Neoplasm Metastasis ,Pre-Clinical Reports ,PI3K/AKT/mTOR pathway ,Cell Proliferation ,Pharmacology ,PI3K/AKT ,Dose-Response Relationship, Drug ,biology ,urogenital system ,Akt/PKB signaling pathway ,Liver Neoplasms ,Cell migration ,biology.organism_classification ,medicine.disease ,migration and invasion ,berbamine ,Oncology ,chemistry ,Berberis ,Cancer research ,Phosphatidylinositol 3-Kinase ,Liver cancer ,Proto-Oncogene Proteins c-akt - Abstract
Berbamine is a bisbenzylisoquinoline alkaloid extracted from Berberis poiretii of Berberis of Berberidaceae. It has been reported that it can significantly inhibit the proliferation of a variety of malignant tumor cells, including liver cancer. However, the effect of berbamine on the invasion and metastasis of liver cancer has not been reported. The present study demonstrated that berbamine inhibited the migration and invasion of SMMC-7721 cells in a concentration-dependent manner and obviously increased the gap junction function and the expression of Cx32 in SMMC-7721 cells compared with control group. However, after silencing Cx32, berbamine had no significant effect on cell invasion and metastasis. Before silencing Cx32, the expression of PI3K and P-AKT were decreased after berbamine treated on SMMC-7721 cells for 24 h. After silencing Cx32, the expression of PI3K and P-AKT were increased in SMMC-7721 cells. The expression of PI3K and P-AKT had no significant effect after berbamine treated on SMMC-7721 cells for 24 h with silencing Cx32. In conclusion, the results of the present study suggest that berbamine could inhibit the SMMC-7721 cell migration and invasion, and its mechanism may be related to the regulation of PI3K/AKT signaling pathway by enhancing the expression of Cx32.
- Published
- 2021
21. Introducing Electrochemically Active Oxygen Species to Boost the Pseudocapacitance of Carbon‐based Supercapacitor
- Author
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Wen Cui Li, Hong Kun Zhuang, Lu Hou, Xiao Ling Dong, You Ren Hu, and Dong Yan
- Subjects
Supercapacitor ,Active oxygen ,Materials science ,Chemical engineering ,chemistry ,Electrochemistry ,Energy density ,chemistry.chemical_element ,Carbon ,Catalysis ,Pseudocapacitance - Published
- 2021
22. Fabrication of Biomolecule–Covalent-Organic-Framework Composites as Responsive Platforms for Smart Regulation of Fermentation Application
- Author
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Yao Chen, Dong Yan, Sainan Zhang, Zhenjie Zhang, Haixin Chen, Fazheng Jin, He Huang, Jiangyue Yu, Shan Qiao, Wenjie Duan, and Yunlong Zheng
- Subjects
chemistry.chemical_classification ,Materials science ,Biomolecule ,food and beverages ,Hydrogen-Ion Concentration ,Microbial contamination ,Environmental variation ,chemistry ,Drug Design ,Fermentation ,General Materials Science ,Biomanufacturing ,Composite material ,Metal-Organic Frameworks ,Nisin ,Control release ,Covalent organic framework - Abstract
Exploration of novel material platforms to protect biological preservatives and realize intelligent regulation during fermentation is of great significance in industry. Herein, we established an intelligent responsive platform by introducing antimicrobial biomolecules (nisin) into rationally designed covalent organic frameworks (COFs), resulting in a new type of "smart formulation", which could responsively inhibit microbial contamination and ensure the orderly progression of the fermentation process. The encapsulated biomolecules retained their activity while exhibiting enhanced stability and pH-responsive releasing process (100% bacteriostatic efficiency at a pH of 3), which can ingeniously adapt to the environmental variation during the fermentation process and smartly fulfill the regulation needs. Moreover, the nisin@COF composites would not affect the fermentation strains. This study will pave a new avenue for the preparation of highly efficient and intelligent antimicrobial agents for the regulation of the fermentation process and play valuable roles in the drive toward green and sustainable biomanufacturing.
- Published
- 2021
23. Dual inhibition of cMET and EGFR by microRNA-338-5p suppresses metastasis of esophageal squamous cell carcinoma
- Author
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Nikki P. Lee, Liang Han, Di Cui, Yun Zhu, Simon Law, George S.W. Tsao, Annie L.M. Cheung, and Dong Dong Yan
- Subjects
0301 basic medicine ,Cancer Research ,Lung Neoplasms ,Esophageal Neoplasms ,Mice, Nude ,Apoptosis ,Matrix metalloproteinase ,Metastasis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Growth factor receptor ,microRNA ,Tumor Cells, Cultured ,medicine ,Animals ,Humans ,Gene silencing ,Neoplasm Invasiveness ,Secretion ,Epidermal growth factor receptor ,Cell Proliferation ,biology ,Chemistry ,General Medicine ,Proto-Oncogene Proteins c-met ,medicine.disease ,Xenograft Model Antitumor Assays ,digestive system diseases ,ErbB Receptors ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Esophageal Squamous Cell Carcinoma ,Signal transduction - Abstract
MicroRNAs, as a group of post-transcriptional regulators, regulate multiple pathological processes including metastasis during tumor development. Here, we demonstrated the metastasis-suppressive function of microRNA (miR)-338-5p in esophageal squamous cell carcinoma (ESCC). Overexpression of miR-338-5p had inhibitory effect on invasive ability of ESCC cells and extracellular matrix degradation, whereas silencing miR-338-5p had opposite effects. Mechanistically, miR-338-5p directly targeted the 3′ untranslated regions of hepatocellular growth factor receptor cMet (cMET) and epidermal growth factor receptor (EGFR). As a result, miR-338-5p inhibited the downstream signaling cascades of cMET and EGFR and repressed cMET- and EGFR-mediated ESCC cell invasion. Re-expression of cMET or EGFR in miR-338-5p overexpressing ESCC cells was sufficient to derepress ESCC cell invasion both in vitro and in vivo. We further showed that such manipulation downregulated the expression and secretion of matrix metalloproteinases 2 and 9, which resulted in impaired extracellular matrix degradation and cell invasion. Most importantly, systemic delivery of miR-338-5p mimic significantly inhibited metastasis of ESCC cells in nude mice. Taken together, our results uncovered a previously unknown mechanism through which miR-338-5p suppresses ESCC invasion and metastasis by regulating cMET/EGFR-matrix metalloproteinase 2/9 axis and highlighted the potential significance of miR-338-5p-based therapy in treating patients with metastatic ESCC.
- Published
- 2021
24. Highly Efficient Hydrogenation of Furfural to Furfuryl Alcohol Catalyzed by Pt Supported on Bi-Metallic MIL-100 (Fe, Mn/Co) MOFs Derivates Prepared by Hydrothermal Polyol Reduction Method
- Author
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Xiqiang Tian, Jiang Li, Wang Sun, Yujun Zhu, Dong Yan, and Lin Luo
- Subjects
chemistry.chemical_classification ,010405 organic chemistry ,General Chemistry ,010402 general chemistry ,Furfural ,01 natural sciences ,Catalysis ,Hydrothermal circulation ,0104 chemical sciences ,Furfuryl alcohol ,chemistry.chemical_compound ,Adsorption ,chemistry ,Polyol ,Selectivity ,Bimetallic strip ,Nuclear chemistry - Abstract
The Pt/(Fe, Mn/Co)-BTC catalysts were fabricated by a facile ultrasonic impregnation assisted hydrothermal polyol reduction method using first MIL-100(Fe, Mn/Co) MOFs as supports and tested for selective hydrogenation of furfural (FAL) to furfuryl alcohol (FOL). Various characterizations including XRD, TG, FT-IR, N2 physical adsorption, ICP-OES, SEM, TEM and XPS were applied for exploring structure changes and surface properties of the catalysts. Over 98% conversion of FAL and 99% selectivity to FOL were achieved over Pt/(Fe, Mn)-BTC and Pt/(Fe, Co)-BTC, moreover, Pt/(Fe, Co)-BTC showed higher turnover frequency (TOF) of 1,044 h−1 and better cycle stability, which are better than other catalysts prepared by using H2, NaBH4, and formaldehyde as reductants. Overall, the formation of highly dispersed small-sized Pt NPs (about 1.7 nm) with much higher content of surface Pt0 on the catalysts is the key of excellent hydrogenation activity due to the strong interaction between Pt NPs and the bimetallic supports.
- Published
- 2021
25. Graphene Oxide/Fe2O3 Nanocomposite as an Efficient Catalyst for Thermal Decomposition of Ammonium Perchlorate via the Vacuum-Freeze-Drying Method
- Author
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Haiyan Zhao, Fan Yang, Jiayun Pei, and Dong Yan
- Subjects
Nanocomposite ,Materials science ,Graphene ,Thermal decomposition ,Oxide ,Iron oxide ,02 engineering and technology ,Surfaces and Interfaces ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Ammonium perchlorate ,01 natural sciences ,0104 chemical sciences ,law.invention ,Nanomaterials ,Catalysis ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,law ,Electrochemistry ,General Materials Science ,0210 nano-technology ,Spectroscopy - Abstract
The combination of graphene oxide (GO) and iron oxide (Fe2O3) may induce property enforcement and application extension. Herein, GO/Fe2O3 nanocomposites were synthesized via the vacuum-freeze-drying method and used for the thermal decomposition of ammonium perchlorate (AP). A series of characterization techniques were applied to elucidate the as-obtained nanomaterial's physicochemical properties. These results show that the treated GO is consistent with the pristine GO after the freeze-drying treatment. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) analyses show that iron oxide nanoparticles are anchored on and between the GO sheets. The catalytical effect investigation on AP with different Fe2O3: GO ratios indicates that the high-temperature decomposition temperature of AP could be decreased by a temperature as high as 77 °C compared to pure AP accompanied by 3 wt % GO/Fe2O3 nanocomposite which proves the high catalytic performance of the nanocomposites. The first-principles calculation was employed to elaborate the synergistic effect, and the findings demonstrate that the presence of graphene in the catalyst can enhance the catalytic effect via reducing the activation energy barrier by ∼17% in the reaction of AP thermal decomposition.
- Published
- 2021
26. Unlocking Rapid and Robust Sodium Storage Performance of Zinc-Based Sulfide via Indium Incorporation
- Author
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Ye Wang, Daliang Fang, Shengyuan A. Yang, Yang Shang, Xue Liang Li, Guangzhao Wang, Mei Er Pam, Dong Yan, Hui Ying Yang, Yumeng Shi, and Yew Von Lim
- Subjects
chemistry.chemical_classification ,Materials science ,Sulfide ,Heteroatom ,Intercalation (chemistry) ,General Engineering ,General Physics and Astronomy ,chemistry.chemical_element ,02 engineering and technology ,Zinc ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Zinc sulfide ,0104 chemical sciences ,Anode ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,General Materials Science ,0210 nano-technology ,Indium - Abstract
Zinc sulfide (ZnS) exhibits promise in sodium-ion batteries (SIBs) because of its low operation voltage and high theoretical specific capacity. However, pristine ZnS is not adequate in realizing rapid and robust sodium storage owing to its low reversibility, poor structure stability, and sluggish kinetics. To date, most efforts focus on utilizing carbonaceous incorporation to improve its electrochemical performances. Nevertheless, it remains an arduous challenge for realizing superior rate capability while obtaining stable cycling. Herein, inspired by the crystal structure of hexagonal ZnIn2S4, which possesses an intrinsic layered feature with larger unit-cell volume versus that of ZnS, indium incorporation is thus deployed as an immediate remedy. In/ex situ investigations combined with density functional theory calculations are conducted to reveal the superior kinetics, high reversibility, and good structure stability of ZnIn2S4. Notably, the formed indium-based derivatives during cycling manifest a Na+ (de)intercalation process, thereby exciting a synergetic mechanism to stabilize electrochemical cycling. As a result, the electrochemical performances of Zn-based sulfide are significantly improved via the indium incorporation. Furthermore, a full cell based on the ZnIn2S4 anode with the superior electrochemical performance is developed. This work provides an effective tactic of heteroatom incorporation for optimizing structure as well as exciting a complementary reaction process toward developing superior anodes for high-performance alkali-ion batteries.
- Published
- 2021
27. A SnOx Quantum Dots Embedded Carbon Nanocage Network with Ultrahigh Li Storage Capacity
- Author
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Zefei Liu, Yanan Zhang, Dong Yan, An-Hui Lu, Fei Cheng, Youwen Ye, and Huanrong Li
- Subjects
Materials science ,Oxide ,General Physics and Astronomy ,chemistry.chemical_element ,02 engineering and technology ,engineering.material ,010402 general chemistry ,Electrochemistry ,01 natural sciences ,law.invention ,chemistry.chemical_compound ,Nanocages ,Coating ,law ,General Materials Science ,General Engineering ,021001 nanoscience & nanotechnology ,Cathode ,0104 chemical sciences ,Anode ,chemistry ,Chemical engineering ,Quantum dot ,engineering ,0210 nano-technology ,Tin - Abstract
Tin-based materials with high specific capacity have been studied as high-performance anodes for energy storage devices. Herein, a SnOx (x = 0, 1, 2) quantum dots@carbon hybrid is designed and prepared by a binary oxide-induced surface-targeted coating of ZIF-8 followed by pyrolysis approach, in which SnOx quantum dots (under 5 nm) are dispersed uniformly throughout the nitrogen-containing carbon nanocage. Each nanocage is cross-linked to form a highly conductive framework. The resulting SnOx@C hybrid exhibits a large BET surface area of 598 m2 g-1, high electrical conductivity, and excellent ion diffusion rate. When applied to LIBs, the SnOx@C reveals an ultrahigh reversible capacity of 1824 mAh g-1 at a current density of 0.2 A g-1, and superior capacities of 1408 and 850 mAh g-1 even at high rates of 2 and 5 A g-1, respectively. The full cell assembled using LiFePO4 as cathode exhibits the high energy density and power density of 335 Wh kg-1 and 575 W kg-1 at 1 C based on the total active mass of cathode and anode. Combined with in situ XRD analysis, the superior electrochemical performance can be attributed to the SnOx-ZnO-C asynchronous and united lithium storage mechanism, which is formed by the well-designed multifeatured construction composed of SnOx quantum dots, interconnected carbon network, and uniformly dispersed ZnO nanoparticles. Importantly, this designed synthesis can be extended for the fabrication of other electrode materials by simply changing the binary oxide precursor to obtain the desired active component or modulating the type of MOFs coating to achieve high-performance LIBs.
- Published
- 2021
28. Fragmenting C60 toward enhanced electrochemical CO2 reduction
- Author
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Dong Yan, Wei Wang, Zhen Peng, Yiyin Huang, and Peng Zeng
- Subjects
Materials science ,Mechanical Engineering ,Doping ,chemistry.chemical_element ,Carbon nanotube ,Electrochemistry ,Photochemistry ,Catalysis ,law.invention ,chemistry ,Mechanics of Materials ,law ,General Materials Science ,Selectivity ,Carbon ,Faraday efficiency - Abstract
Carbon-based metal-free catalysts exhibit great applications in electrochemical CO2 reduction (ECR), while most studies merely focus on large-sized carbons with limited ratio of surface atoms for engineering to create surface active centers. Here, we report a joint treatment of C60 by heating and plasma to induce a dramatic performance promotion during ECR. The electrochemical measurements indicate the Faraday efficiency for ECR toward CO production kept at high level over 80% in a wide potential region from − 0.4 to − 0.7 V versus RHE, with the highest value reaching 96.8%. The physical characterization reveals that fragmentation of C60 occurs together with N/O doping, both of which could induce change in electron structure, causing the formation of *COOH intermediate and ultimately leading to the optimized activity and selectivity for CO production. This treatment route was further revealed to be applicative for other carbon materials, e.g., single-walled carbon nanotube, to promote its ECR activity and selectivity.
- Published
- 2021
29. SARS-CoV-2 main protease suppresses type I interferon production by preventing nuclear translocation of phosphorylated IRF3
- Author
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Sin-Yee Fung, Dong-Yan Jin, Huayue Lin, Man Lung Yeung, and Kam-Leung Siu
- Subjects
medicine.medical_treatment ,viruses ,NSP5 ,Applied Microbiology and Biotechnology ,03 medical and health sciences ,TANK-binding kinase 1 ,Interferon ,Chlorocebus aethiops ,medicine ,Animals ,Humans ,Phosphorylation ,skin and connective tissue diseases ,Molecular Biology ,Vero Cells ,Ecology, Evolution, Behavior and Systematics ,Coronavirus 3C Proteases ,030304 developmental biology ,Cell Nucleus ,0303 health sciences ,Protease ,Chemistry ,SARS-CoV-2 ,fungi ,virus diseases ,COVID-19 ,SARS-CoV ,Cell Biology ,biochemical phenomena, metabolism, and nutrition ,IRF3 ,Type I interferon production ,Cell biology ,Protein Transport ,type I interferons ,Interferon Type I ,Vero cell ,Interferon Regulatory Factor-3 ,3C-like protease ,Interferon type I ,Developmental Biology ,medicine.drug ,Research Paper - Abstract
Suppression of type I interferon (IFN) response is one pathological outcome of the infection of highly pathogenic human coronaviruses. To effect this, severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 encode multiple IFN antagonists. In this study, we reported on the IFN antagonism of SARS-CoV-2 main protease NSP5. NSP5 proteins of both SARS-CoV and SARS-CoV-2 counteracted Sendai virus-induced IFN production. NSP5 variants G15S and K90R commonly seen in circulating strains of SARS-CoV-2 retained the IFN-antagonizing property. The suppressive effect of NSP5 on IFN-β gene transcription induced by RIG-I, MAVS, TBK1 and IKKϵ suggested that NSP5 likely acts at a step downstream of IRF3 phosphorylation in the cytoplasm. NSP5 did not influence steady-state expression or phosphorylation of IRF3, suggesting that IRF3, regardless of its phosphorylation state, might not be the substrate of NSP5 protease. However, nuclear translocation of phosphorylated IRF3 was severely compromised in NSP5-expressing cells. Taken together, our work revealed a new mechanism by which NSP5 proteins encoded by SARS-CoV and SARS-CoV-2 antagonize IFN production by retaining phosphorylated IRF3 in the cytoplasm. Our findings have implications in rational design and development of antiviral agents against SARS-CoV-2.
- Published
- 2021
30. Role of Hakai in m6A modification pathway in Drosophila
- Author
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Dong Yan, Lijuan Ma, Zhongwen Lu, Lifeng Zhang, Yanhua Wang, Jian Guo, Hang Ren, Decai Mao, and Lijun Lu
- Subjects
0301 basic medicine ,Messenger RNA ,Multidisciplinary ,biology ,Chemistry ,Science ,Mutant ,Alternative splicing ,fungi ,General Physics and Astronomy ,RNA ,General Chemistry ,Plasma protein binding ,Methylation ,General Biochemistry, Genetics and Molecular Biology ,Cell biology ,Ubiquitin ligase ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,RNA splicing ,biology.protein ,030217 neurology & neurosurgery - Abstract
N6-methyladenosine (m6A), the most abundant internal modification in eukaryotic mRNA, is installed by a multi-component writer complex; however, the exact roles of each component remain poorly understood. Here we show that a potential E3 ubiquitin ligase Hakai colocalizes and interacts with other m6A writer components, and Hakai mutants exhibit typical m6A pathway defects in Drosophila, such as lowered m6A levels in mRNA, aberrant Sxl alternative splicing, wing and behavior defects. Hakai, Vir, Fl(2)d and Flacc form a stable complex, and disruption of either Hakai, Vir or Fl(2)d led to the degradation of the other three components. Furthermore, MeRIP-seq indicates that the effective m6A modification is mostly distributed in 5’ UTRs in Drosophila, in contrast to the mammalian system. Interestingly, we demonstrate that m6A modification is deposited onto the Sxl mRNA in a sex-specific fashion, which depends on the m6A writer. Together, our work not only advances the understanding of mechanism and regulation of the m6A writer complex, but also provides insights into how Sxl cooperate with the m6A pathway to control its own splicing.
- Published
- 2021
31. Millimeter-sized few-layer graphene sheets with aligned channels for fast lithium-ion charging kinetics
- Author
-
Yu-Qi Zhou, Xiao-Ling Dong, Wen-Cui Li, Dong Yan, Guang-Ping Hao, and An-Hui Lu
- Subjects
Solid-state chemistry ,Fabrication ,Materials science ,Graphene ,Energy Engineering and Power Technology ,Nanoparticle ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Anode ,law.invention ,Fuel Technology ,chemistry ,Chemical engineering ,law ,Electrochemistry ,Lithium ,0210 nano-technology ,Porosity ,Dispersion (chemistry) ,Energy (miscellaneous) - Abstract
Assembly of the top-down graphene units mostly results in 3D porous structure with randomly organized pores. The direct bottom-up synthesis of macroscopic 2D graphene sheets with organized pores are long sought in materials chemistry field, but rarely achieved. Herein, we present a self-catalysis-assisted bottom-up route using L-glutamic acid and iron chloride as starting materials for the fabrication of the millimeter-sized few-layer graphene sheets with aligned porous channels parallel to the 2D direction. The amino- and carboxyl-functional groups in L-glutamic acid can coordinate with iron cations, thus allowing an atomic dispersion of iron cations. The pyrolysis thus initiated the growth of graphene catalyzed by in-situ generated iron nanoparticles, and a dynamic flow of iron nanoparticles eventually led to the formation of millimeter-sized few-layer graphene sheets with aligned channels (60–85 nm in diameter). Used as anodes in lithium-ion batteries, these graphene sheets showed a good rate capability (142 mA h g−1 at 2 A g−1) and high capacity retention of 93% at 2 A g−1 after 1200 cycles. Kinetic analysis revealed that lithium ions storage was dominated by diffusion behavior and capacitive behavior together, in that graphene sheets with aligned channels could accelerate electron transfer and shorten lithium ions transport pathway. This work provides a novel approach to prepare unique porous graphene materials with specific structure for energy storage.
- Published
- 2021
32. Performance of Al2O3 particle reinforced glass-based seals in planar solid oxide fuel cells
- Author
-
Dong Yan, Jiajun Yang, Jian Li, Bo Chi, Jian Pu, and Ruizhu Li
- Subjects
010302 applied physics ,Materials science ,Process Chemistry and Technology ,Delamination ,Oxide ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Operating temperature ,chemistry ,Phase (matter) ,visual_art ,0103 physical sciences ,Materials Chemistry ,Ceramics and Composites ,Shear strength ,visual_art.visual_art_medium ,Ceramic ,Composite material ,0210 nano-technology ,Power density ,Leakage (electronics) - Abstract
Glass-based materials are usually considered as excellent seals for jointing adjacent components in planar solid oxide fuel cells, but the uncontrollable crystallization in the glass may cause delamination and micro-cracks in such seals. To solve this problem, Al2O3 ceramic particles were added to a BaO–CaO–Al2O3–B2O3–SiO2 glass system to reduce negative effects caused by crystalline phase on the gas tightness and the joint strength in the seals. At an operating temperature of 750 °C, the glass-based seals with 20 wt% Al2O3 addition (GA80) exhibited extremely low leakage rates (~0.002 sccm/cm under an input gas pressure of 13.6 kPa) and higher shear strength (3.31 MPa). The Al2O3 ceramic addition and the crystalline phase BaAl2Si2O8 reinforced the glass matrix. Further thermal cycle analyses indicated that leakage rates for the GA80 seals remained at around 0.0025 sccm/cm after 10 thermal cycles, which was consistent with minor microstructural change and good interface bonding. Single cell testing with of GA80 seals was performed and the results demonstrated stable electrochemical performance through 6 thermal cycles at an open circuit voltage of 1.16–1.18 V, as well as a power density above 546 mW/cm2 at a current density of 925 mA/cm2. These results showed the high thermal cycle stability of the glass/Al2O3 composite seals in intermediate temperature planar solid oxide fuel cells.
- Published
- 2021
33. Baicalein mediates anticancer effect on cholangiocarcinoma through co-targeting the AKT/NF-κB and STAT3 signaling pathway
- Author
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Dan-Ling Chen, Jin Shang, Si-Yang Li, Peng Han, Hui-Qi Li, Dong-Yan Shen, Rui Fan, Shi-Yang Zhang, and Rui-Han Li
- Subjects
Cell cycle checkpoint ,biology ,Chemistry ,Bioengineering ,Cell cycle ,biology.organism_classification ,Applied Microbiology and Biotechnology ,Biochemistry ,Stat3 Signaling Pathway ,Baicalein ,IκBα ,chemistry.chemical_compound ,Apoptosis ,Cancer research ,Scutellaria baicalensis ,Protein kinase B - Abstract
Baicalein, the primary flavonoid derived from the roots of Scutellaria baicalensis Georgi, is known to exhibit anticancer effects against different types of cancer. However, the anticancer activity and underlying mechanisms of baicalein on cholangiocarcinoma (CCA) have not been elucidated. Here, our results showed that baicalein inhibited the viability and proliferation of CCA cells in a dose and time-dependent manner. Besides, baicalein also induced cell apoptosis and cell cycle arrest by regulating cell cycle associated proteins and Bcl-2 family proteins expression. More importantly, baicalein also suppressed the migration and invasion by reducing the expression of MMP-2 and MMP-9 proteins, as well as decreased the aerobic glycolysis of CCA cells by reducing Hexokinase and Glucose-6-phosphate. In the mechanistic study, the phosphorylation of IκBα, AKT and STAT3 were all significantly decreased by baicalein in vitro and in vivo. Furthermore, baicalein also inhibited the tumor growth in subcutaneous xenograft model of mice. These findings indicate that baicalein has potent anticancer activities by suppressing multiple malignant phenotypes and most probably through inhibiting the activation of the AKT/NF-κB and STAT3 signaling pathway. Thus, it has the potential to be developed as an anticancer agent to enhance clinical standards of care for patients with CCA.
- Published
- 2021
34. HDAC1-mediated deacetylation of HIF1α prevents atherosclerosis progression by promoting miR-224-3p-mediated inhibition of FOSL2
- Author
-
Hong-Yu Yang, Kazuo Sugimoto, Dong Yan, Yue-Bo Song, Ji-Yue Liu, Hao Wang, Hao Lu, Wan-Yong Yang, Tian-Yu Zou, and Si Shen
- Subjects
miR-224-3p ,0301 basic medicine ,Cell ,Inflammation ,FOSL2 ,03 medical and health sciences ,0302 clinical medicine ,endothelial cell apoptosis ,Drug Discovery ,microRNA ,medicine ,HIF1α ,reactive oxygen species ,chemistry.chemical_classification ,Reactive oxygen species ,Chemistry ,lcsh:RM1-950 ,HDAC1 ,Endothelial stem cell ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,medicine.anatomical_structure ,Apoptosis ,Acetylation ,030220 oncology & carcinogenesis ,Cancer research ,Molecular Medicine ,Original Article ,atherosclerosis ,medicine.symptom ,Intracellular - Abstract
We intended to characterize functional relevance of microRNA (miR)-224-3p in endothelial cell (EC) apoptosis and reactive oxygen species (ROS) accumulation in atherosclerosis, considering also the integral involvement of histone deacetylase 1 (HDAC1)-mediated hypoxia-inducible factor-1α (HIF1α) deacetylation. The binding affinity between miR-224-3p and Fos-like antigen 2 (FOSL2) was predicted and validated. Furthermore, we manipulated miR-224-3p, FOSL2, HDAC1, and HIF1α expression in oxidized low-density lipoprotein (ox-LDL)-induced ECs, aiming to clarify their effects on cell activities, inflammation, and ROS level. Additionally, we examined the impact of miR-224-3p on aortic atherosclerotic plaque and lesions in a high-fat-diet-induced atherosclerosis model in ApoE−/− mice. Clinical atherosclerotic samples and ox-LDL-induced human aortic ECs (HAECs) exhibited low HDAC1/miR-224-3p expression and high HIF1α/FOSL2 expression. miR-224-3p repressed EC cell apoptosis, inflammatory responses, and intracellular ROS levels through targeting FOSL2. HIF1α reduced miR-224-3p expression to accelerate EC apoptosis and ROS accumulation. Moreover, HDAC1 inhibited HIF1α expression by deacetylation, which in turn enhanced miR-224-3p expression to attenuate EC apoptosis and ROS accumulation. miR-224-3p overexpression reduced atherosclerotic lesions in vivo. In summary, HDAC1 overexpression may enhance the anti-atherosclerotic and endothelial-protective effects of miR-224-3p-mediated inhibition of FOSL2 by deacetylating HIF1α, underscoring a novel therapeutic insight against experimental atherosclerosis., Graphical Abstract, The study mainly addressed the suppressive role of HDAC1-mediated deacetylation of HIF1α in atherosclerosis progression, which was associated with miR-224-3p-mediated inhibition of FOSL2, thus highlighting a potential therapeutic action against atherosclerosis.
- Published
- 2021
35. High-performance La0.5(Ba0.75Ca0.25)0.5Co0.8Fe0.2O3-δ cathode for proton-conducting solid oxide fuel cells
- Author
-
Jian Pu, Jian Li, Jun Yang, Kai Li, Bo Chi, Lichao Jia, Dong Yan, and Dong Xie
- Subjects
Materials science ,Renewable Energy, Sustainability and the Environment ,Oxide ,Energy Engineering and Power Technology ,02 engineering and technology ,Electrolyte ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Electrochemistry ,01 natural sciences ,Cathode ,Dissociation (chemistry) ,0104 chemical sciences ,law.invention ,Catalysis ,chemistry.chemical_compound ,Fuel Technology ,chemistry ,Chemical engineering ,law ,Electrical resistivity and conductivity ,0210 nano-technology ,Power density - Abstract
La0.5(Ba0.75Ca0.25)0.5Co0.8Fe0.2O3-δ, a simple perovskite cathode material with high electrical conductivity (940 S cm−1 at 600 °C) and impressive surface catalytic activity, was prepared and used in proton-conducting solid oxide fuel cells. As its thermal expansion coefficient is higher than that of the electrolyte material BaZr0.1Ce0.7Y0.1Yb0.1O3-δ, they were combined and used as a composite cathode. The crystal structure, chemical compatibility, electrical conductivity, cell performance, and the oxygen reduction reaction of the cathode material were explored, and we found that the single fuel cell developed with the composite cathode achieved excellent electrochemical performance, with both a low polarization resistance and high peak power density (0.044 Ω cm2 and 1102 mW cm−2 at 750 °C, respectively). Outstanding stability was also achieved, as indicated by a long-term 100-h test. Additionally, the rate-limiting steps of the oxygen reduction reaction were the oxygen adsorption, dissociation, and diffusion processes.
- Published
- 2021
36. A 3-to-40-V Automotive-Use GaN Driver With Active Bootstrap Balancing and V SW Dual-Edge Dead-Time Modulation Techniques
- Author
-
Min-Kyu Song, D. Brian Ma, Dong Yan, Joseph Sankman, and Xugang Ke
- Subjects
Physics ,chemistry.chemical_compound ,chemistry ,Modulation ,Biasing ,Gallium nitride ,Propagation delay ,Electrical and Electronic Engineering ,Edge (geometry) ,Dead time ,Atomic physics ,Voltage ,Power (physics) - Abstract
This article presents a GaN driver operating at a switching frequency variable from 10 to 30 MHz to achieve reliable and efficient power conversion for automotive electronics applications. An active bootstrap (BST) balancing (ABB) technique is presented to stabilize the BST rail voltage by sensing $V_{\mathrm{ SW}}$ and controlling BST rail charging. Meanwhile, a $V_{\mathrm{ SW}}$ dual-edge dead-time ( $t_{\mathrm{ dead}}$ ) modulation senses instant $V_{\mathrm{ IN}}$ and $I_{O}$ and generates optimal $t_{\mathrm{ dead}}$ for $V_{\mathrm{ SW}}$ trailing and leading edges, achieving zero-voltage switching (ZVS) turn-on for GaN power switches. To operate at high frequency and retain high efficiency, pulse-based dynamic up- and down-level shifters are developed to achieve sub-nanosecond propagation delay with a quiescent current of 0.5 $\mu \text{A}$ . To validate this work, a switching power converter prototype is implemented using a 0.35- $\mu \text{m}$ high-voltage (HV) BCD process. It maintains a constant 5.1-V BST rail voltage to prevent GaN FET from destructive breakdown and accomplishes 0.9-to-3.7-ns $t_{\mathrm{ dead}}$ and 3.7-to-10.4-ns $t_{\mathrm{ dead}}$ for $V_{\mathrm{ SW}}$ trailing and leading edge, respectively, in response to load current $I_{O}$ change from 0.01 to 1.2 A. The proposed techniques jointly improve the efficiency by 8.3%, which peaks at 90.7% for 12-to-5-V conversion and 88% for 40-to-5-V conversion.
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- 2021
37. Rational Fabrication of Crystalline Smart Materials for Rapid Detection and Efficient Removal of Ozone
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Zhifang Wang, Peng Cheng, Yao Chen, Zhenjie Zhang, and Dong Yan
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High energy ,Ozone ,Fabrication ,Materials science ,Moisture ,010405 organic chemistry ,Nanotechnology ,General Chemistry ,General Medicine ,010402 general chemistry ,Smart material ,01 natural sciences ,Rapid detection ,Catalysis ,0104 chemical sciences ,Characterization (materials science) ,chemistry.chemical_compound ,chemistry ,Covalent organic framework - Abstract
Traditional ozone sensing and removal materials still suffer from high energy consumption and low efficiency. Thus, seeking new ozone-responsive materials with high efficiency and broad working conditions is of great significance. Herein, we first developed covalent organic frameworks (COFs) for smart sensing and efficient removal of ozone. Notably, imine-based COFs possess dramatically fast optical responses (
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- 2021
38. Colloidal quantum dots and metal halide perovskite hybridization for solar cell stability and performance enhancement
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Zhe Li, Dong Yan, Mengxia Liu, and Bo Hou
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Materials science ,Renewable Energy, Sustainability and the Environment ,Chalcogenide ,Bilayer ,Halide ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,law.invention ,Metal ,chemistry.chemical_compound ,Colloid ,chemistry ,law ,visual_art ,Solar cell ,visual_art.visual_art_medium ,General Materials Science ,0210 nano-technology ,Performance enhancement ,Perovskite (structure) - Abstract
Metal halide perovskites and colloidal quantum dots (QDs) are two emerging classes of photoactive materials that have attracted considerable attention for next-generation high-performance solution-processed solar cells. In particular, the hybridization of these two types of materials has recently demonstrated remarkable performance enhancement due to the complementary nature of the two constituents. In this review, we will highlight the recent progress of QDs and perovskite hybridization in solar cell applications. More specifically, the unique properties of monophase perovskite QDs will be summarised, and are demonstrated by homogeneously hybridizing perovskite QDs into the perovskite lattice. We also discuss the recent progress in heterogeneously hybridizing discrete colloidal QDs into perovskite layers which results in significant enhancement in perovskite film stability as well as corresponding solar cell performance improvement. PbS QDs, other chalcogenide QDs, and emerging two-dimensional QDs are further accounted through multiple methods, such as constructing bilayer architectures and core–shell structures or blending multiple QDs into perovskite layers. In the end, an outlook perspective of this field has been proposed to point out several challenges and possible solutions.
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- 2021
39. The design of flower-like C–MnO2 nanosheets on carbon cloth toward high-performance flexible zinc-ion batteries
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Hai-Xu Zhao, Fei Li, Hua-Yu Zhang, Gui-Zhong Li, Yi-Lin Liu, Dong Yan, Hui Ying Yang, and Gui-Gen Wang
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Flexibility (engineering) ,Materials science ,Fabrication ,Renewable Energy, Sustainability and the Environment ,chemistry.chemical_element ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,Cathode ,0104 chemical sciences ,law.invention ,chemistry ,law ,General Materials Science ,0210 nano-technology ,Carbon ,Layer (electronics) ,Electrical conductor ,Current density - Abstract
With the increased development of wearable electronics, flexible zinc ion batteries (ZIBs) have attracted widespread attention owing to their low cost, superior safety, and simple fabrication process. Nevertheless, the limited active mass loading and low specific capacity remain challenges to be overcome. Herein, an effective in situ growth strategy is used to boost the mass loading and specific capacity of flexible ZIBs. We employed a mussel-derived polydopamine layer as an inductive catalyst to prompt the growth of flower-like δ-MnO2 on carbon cloth, which demonstrated strong adhesion to the surface, high mass loading, and excellent flexibility. In addition, the formed 3D conductive and supporting networks contribute to the realization of superior long cycle life, as well as a high specific capacity. As a result, the as-obtained flexible cathode can achieve a high initial discharge capacity of 1.3 mA h cm−2 at a current density of 1 mA cm−2 and a reversible capacity of 0.25 mA h cm−2 at 10 mA cm−2 for 10 000 cycles. Moreover, its reaction mechanism in ZIBs was analyzed via a series of ex situ characterization techniques, and the results suggest that the charge–discharge behavior is determined based on the Zn2+ (de-)intercalation in the δ-MnO2 interlayer. Remarkably, the as-developed flexible cathode is assembled into a flexible quasi-solid-state device, which shows excellent flexibility and can easily power one LED-based device, which has significant application prospects in wearable electronics. This work provides a novel tactic for the design of flexible batteries with high active mass loading and excellent electrochemical performance via employing polydopamine as an inductive catalyst.
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- 2021
40. In silico structure-based discovery of a SARS-CoV-2 main protease inhibitor
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Yaoqiang Huang, Lei Wen, Kwok-Yung Yuen, Chris Chun-Yiu Chan, Cuiting Luo, Jianli Cao, Kaiming Tang, Zi-Wei Ye, Dong-Yan Jin, Feifei Yin, Hin Chu, Kenn Ka-Heng Chik, Ronghui Liang, Jasper Fuk-Woo Chan, Jessica Oi-Ling Tsang, Jian-Piao Cai, and Shuofeng Yuan
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Protease ,Antagonists & inhibitors ,Drug discovery ,medicine.drug_class ,viruses ,In silico ,medicine.medical_treatment ,Histone deacetylase inhibitor ,Cell Biology ,Biology ,Applied Microbiology and Biotechnology ,Virology ,Chemical library ,chemistry.chemical_compound ,Trichostatin A ,chemistry ,medicine ,Protease inhibitor (pharmacology) ,skin and connective tissue diseases ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Research Paper ,Developmental Biology ,medicine.drug - Abstract
The Coronavirus Disease 2019 (COVID-19) pandemic caused by the novel lineage B betacoroanvirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has resulted in significant mortality, morbidity, and socioeconomic disruptions worldwide. Effective antivirals are urgently needed for COVID-19. The main protease (Mpro) of SARS-CoV-2 is an attractive antiviral target because of its essential role in the cleavage of the viral polypeptide. In this study, we performed an in silico structure-based screening of a large chemical library to identify potential SARS-CoV-2 Mpro inhibitors. Among 8,820 compounds in the library, our screening identified trichostatin A, a histone deacetylase inhibitor and an antifungal compound, as an inhibitor of SARS-CoV-2 Mpro activity and replication. The half maximal effective concentration of trichostatin A against SARS-CoV-2 replication was 1.5 to 2.7µM, which was markedly below its 50% effective cytotoxic concentration (75.7µM) and peak serum concentration (132µM). Further drug compound optimization to develop more stable analogues with longer half-lives should be performed. This structure-based drug discovery platform should facilitate the identification of additional enzyme inhibitors of SARS-CoV-2.
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- 2021
41. Investigation of the Physical Properties and Clinical Application of Embosphere Microspheres
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Ying Xie, Hui Liu, Zhangping Zhao, Songwei Li, Dong Yan, Xingming Xu, Yinbao Hu, and Youjian Zou
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Carcinoma, Hepatocellular ,Embosphere Microspheres ,medicine.medical_treatment ,Microsphere ,Hepatic Artery ,Arsenic Trioxide ,Drug Discovery ,medicine ,Humans ,Pharmacology (medical) ,Embolization ,Chemoembolization, Therapeutic ,Particle Size ,Physiological saline ,Pharmacology ,Drug Carriers ,Chemistry ,Liver Neoplasms ,Angiography ,General Medicine ,Tumor control ,Microspheres ,Smooth surface ,Chemotherapy Embolization ,Infectious Diseases ,Oncology ,Gelatin ,Fluorouracil ,Swelling ,medicine.symptom ,Oils ,Biomedical engineering - Abstract
Background: The aim of this study was to understand physical characteristics of Embosphere microspheres for the clinical use of microsphere chemotherapy embolization of liver cancer. Methods: The morphology of Embosphere microspheres in different states, including static, oscillating, and in a magnetic field was observed with the naked eye. Ninety-five patients diagnosed with primary hepatocellular carcinoma (HCC) were separated into 3 groups based on the types of embolic material as follows: 32 cases of sole microspheres, 34 cases of iodinated oil (17 cases with additional application of gelatin sponge particle), and 29 cases of iodinated oil + Embosphere microspheres. Results: The diameter of the microspheres ranged from 100 to 300 μm, with a sedimentation rate υ = 0.0375 cm/s in physiological saline. The diameter of microspheres ranged from 300 to 500 μm, with a sedimentation rate υ = 0.1875 cm/s. The swelling rate of microspheres was 90%. Microspheres showed nondirectional movement in a 1.5- or 3.0-T magnetic field during magnetic resonance imaging. A volumetric ratio of 1:1.4–1:1.5 between microspheres and contrast agent resulted in optimal suspension properties. Microspheres appeared circular with a smooth surface upon water adsorption. Microsphere embolism was observable in blood vessels of pathological sections. The surface of microspheres can adsorb 5-fluorouracil and arsenic trioxide. There are statistically significant differences in local-regional tumor control conditions among patients treated with sole microspheres, iodinated oil, and iodinated oil + microspheres during transarterial chemoembolization. Conclusions: Embosphere microspheres can be used to embolize patients with rupture and hemorrhage of HCC. Embosphere microsphere embolization is superior to iodinated oil and iodinated oil + microsphere for HCC.
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- 2021
42. Tungsten disulfide-reduced GO/CNT aerogel: a tuned interlayer spacing anode for efficient water desalination
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Lu Guo, Shaozhuan Huang, Zhi Yi Leong, Pablo Valdivia y Alvarado, Hui Ying Yang, Dong Yan, Mohsen Saeedikhani, Ye Wang, Sareh Vafakhah, and Lijuan Hou
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Materials science ,Renewable Energy, Sustainability and the Environment ,Capacitive deionization ,Tungsten disulfide ,Aerogel ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Chloride ,Desalination ,Cathode ,0104 chemical sciences ,law.invention ,Ion ,Anode ,chemistry.chemical_compound ,Chemical engineering ,chemistry ,law ,medicine ,General Materials Science ,0210 nano-technology ,medicine.drug - Abstract
Owing to the favorable energy efficiency and environmental compatibility, capacitive deionization (CDI) has been greatly developed as a potential technology to overcome the ever-growing global water shortage. Herein, an effective and simple strategy is reported to boost the desalination performance by 30 percent through an expansion of interlayer spacing. The final redox active hybrid CDI material consists of WS2 nanoflowers embedded in a highly conductive free-standing rGO-CNT (WS2/rGO-CNT) aerogel as an anode and rGO-CNT aerogel as a cathode. WS2 is applied as a redox-active material for sodium ion intercalation, and three-dimensional (3D) rGO-CNT facilitates the diffusion of the ions to boost the reaction kinetics. Upon charging/discharging, sodium ions intercalate/deintercalate into/from the WS2 lattice structure which is confirmed via in situ XRD measurements, and chloride ions are physically adsorbed/desorbed by the rGO-CNT aerogel. Benefiting from the synergistic effect between the highly conductive and porous rGO-CNT framework and WS2 nanoflowers with a tuned structure, the as-assembled HCDI device enables stable desalination performance with a superior removal capacity of 80 mg g−1, and an excellent removal rate of 3.9 mg g−1 min−1.
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- 2021
43. Quasi-solid electrolyte developed on hierarchical rambutan-like γ-AlOOH microspheres with high ionic conductivity for lithium ion batteries
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Dong Yan, Huiling Zhao, Hui Ying Yang, Xiaolei Wu, Gao Mengmeng, Shuwei Sun, Shuhong Yi, Caiyan Yu, and Ying Bai
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Battery (electricity) ,Materials science ,chemistry.chemical_element ,02 engineering and technology ,Electrolyte ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Ion ,chemistry ,Chemical engineering ,Specific surface area ,Hydrothermal synthesis ,Ionic conductivity ,General Materials Science ,Lithium ,0210 nano-technology ,Electrochemical window - Abstract
Upgrading liquid electrolytes with all-solid-state electrolytes (ASEs) or quasi-solid-state electrolytes (QSEs) for solid-state batteries (SBs) have emerged not only to address the intrinsic disadvantages of traditional liquid lithium ion batteries, but also to offer more possibilities for the development of new battery chemistries. In this work, a novel rambutan-like yolk–shell-structured porous γ-AlOOH microsphere with a large specific surface area of 262.92 m2 g−1 was firstly obtained by a simple hydrothermal synthesis route, which was then utilized as a robust framework to assemble QSE via encapsulating abundant liquid electrolyte (LE). The obtained γ-AlOOH-QSE exhibits a high ionic conductivity of 4.0 × 10−3 S cm−1, a large lithium ion transference number (tLi+) of 0.76, as well as a wide electrochemical window of 4.72 V vs. Li/Li+. Moreover, the assembled cell of LiFePO4/γ-AlOOH-QSE/Li could maintain a high specific capacity of 144.4 mA h g−1 even after 120 cycles with almost negligible capacity decay, which could be mainly attributed to the excellent interfacial compatibility, prominent performance in suppressing lithium dendrite growth upon cycling (rigid characteristic), as well as the high ionic conductivity of γ-AlOOH-QSE (intrinsic advantage). This work could not only expand the applications of QSE with cost-effective aluminum-based oxides with facile fabrication strategy, but also will shed light on the construction of SEs with more integrated QSEs and ASEs in the field of advanced energy storage.
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- 2021
44. Synthesis and properties of colorless copolyimides derived from 4,4′-diaminodiphenyl ether-based diamines with different substituents
- Author
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Lian Ruhe, Dong Yan, Qiuyu Zhang, Xingfeng Lei, Yuyang Xiao, Shuyu Xue, Guo Xiong, and Zixiang Zhang
- Subjects
Materials science ,Polymers and Plastics ,Organic Chemistry ,Bioengineering ,Ether ,Young's modulus ,Biochemistry ,Flexible electronics ,chemistry.chemical_compound ,symbols.namesake ,chemistry ,Chemical engineering ,Flexible display ,Ultimate tensile strength ,symbols ,Solubility ,Glass transition ,Polyimide - Abstract
Colorless polyimide (PI) films with outstanding mechanical and thermal properties are highly demanded in flexible electronics. Herein three 4,4′-diaminodiphenyl ether (ODA)-based diamines with different substituents (–CH3, –CF3) and substitution positions (ortho and meta positions) were synthesized. After reacting with a combination of 1,2,4,5-cyclohexanetetracarboxylic dianhydride (CHDA) and 4,4′-(hexafluoroisopropylidene)diphthalic anhydride (6FDA) in a typical sequential reaction manner, four highly transparent copolyimide films with excellent mechanical strength, high heat-resistance and superior fracture toughness were fabricated. A copolyimide film made using m-3F-ODA exhibits a fairly high transmittance at 400 nm of up to 86.8%, tensile strength as high as 102 MPa, tensile modulus of 2.76 GPa and glass transition temperature above 308 °C. The desirable solubility enables the prepared PIs to be readily cast from solution into robust films. All these encouraging results make these PI films potential substrates in place of ultrathin glass in flexible displays.
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- 2021
45. Engineering COFs as smart triggers for rapid capture and controlled release of singlet oxygen
- Author
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Peng Cheng, Yao Chen, Dong Yan, En Lin, Zhenjie Zhang, Fazheng Jin, Shan Qiao, Fanhao Xiong, Yi Yang, and Zhifang Wang
- Subjects
chemistry.chemical_classification ,Anthracene ,Materials science ,Renewable Energy, Sustainability and the Environment ,Singlet oxygen ,Nanotechnology ,General Chemistry ,Polymer ,Smart material ,Controlled release ,Characterization (materials science) ,chemistry.chemical_compound ,chemistry ,General Materials Science ,Porous medium ,Covalent organic framework - Abstract
Capture and controlled release of singlet oxygen (1O2) are of great significance but very challenging due to its very short lifetime and high reactivity. Addressing this challenge, we rationally designed and fabricated a highly crystalline, robust, and porous three-dimensional covalent organic framework (3D COF) by installing functional anthracene moieties on its skeleton for 1O2 related applications. Attributed to its open networks, regular channels, and light skeletal density, the active anthracene sites in the 3D COF can be fast and fully accessed, hence affording superior performance compared with other contrastive materials such as 2-dimensional COFs and amorphous polymers. Notably, the 3D COF displays the current record-high 1O2 capture rate among all reported porous materials, verified by various characterization techniques and rational analysis. Furthermore, we used the 3D COF platform as a stimuli-responsive smart material for anti-fake applications. This study not only provides an outstanding platform for 1O2 capture and release but also extends the application scopes of COFs.
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- 2021
46. Significance of serglycin and its binding partners in autocrine promotion of metastasis in esophageal cancer
- Author
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Jun Zhang, Di Cui, Alfred King-Yin Lam, Simon Law, Yun Zhu, Nikki P. Lee, Kin Tak Chan, Sai Wah Tsao, Annie L.M. Cheung, Dong Dong Yan, Wen Wen Xu, Bin Li, and Daisy K Y Shum
- Subjects
Male ,0301 basic medicine ,Esophageal Neoplasms ,Carcinogenesis ,Serglycin ,Vesicular Transport Proteins ,Mice, Nude ,Medicine (miscellaneous) ,Proximity ligation assay ,midkine ,Metastasis ,Proto-Oncogene Proteins c-myc ,03 medical and health sciences ,0302 clinical medicine ,Cell Movement ,Cell Line, Tumor ,Biomarkers, Tumor ,medicine ,metastasis ,Animals ,Humans ,Autocrine signalling ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Midkine ,Mice, Inbred BALB C ,Tissue microarray ,biology ,Chemistry ,CD44 ,Cancer ,Middle Aged ,Prognosis ,medicine.disease ,esophageal squamous cell carcinoma ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Autocrine Communication ,Hyaluronan Receptors ,030104 developmental biology ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,biomarker ,Matrix Metalloproteinase 2 ,Female ,Proteoglycans ,Research Paper ,Signal Transduction - Abstract
Rationale: Little is known about the roles of proteoglycans in esophageal cancer. This study aims to investigate the roles and mechanisms of serglycin (SRGN) proteoglycan in promoting metastasis of esophageal squamous cell carcinoma (ESCC). Methods: Reverse phase protein array analysis was used to identify activated signaling pathways in SRGN-overexpressing cells. Chemokine array was used to identify differentially secreted factors from SRGN-overexpressing cells. Binding between SRGN and potential interacting partners was evaluated using proximity ligation assay and co-immunoprecipitation. The glycosaminoglycan (GAG) chains of SRGN were characterized using fluorophore-assisted carbohydrate electrophoresis. Tissue microarray and serum samples were used to determine the correlation of SRGN expression with clinicopathological parameters and patient survival. Results: In vitro and in vivo experiments showed that SRGN promoted invasion and metastasis in ESCC via activating ERK pathway, stabilizing c-Myc and upregulating the secretion of matrix metalloproteinases. SRGN-knockdown suppressed tumorigenic hallmarks. These SRGN-elicited functions were carried out in an autocrine manner by inducing the secretion of midkine (MDK), which was further identified as a novel binding partner of SRGN for the formation of a SRGN/MDK/CD44 complex. In addition, SRGN interacted with MDK and matrix metalloproteinase 2 in ESCC via its GAG chains, which were mainly decorated with chondroitin sulfate comprising of ∆di-4S and ∆di-6S CS. Clinically, high expression of serum SRGN in serum of patients with ESCC was an independent prognostic marker for poor survival. Conclusions: This study provides the first evidence that elevated serum SRGN has prognostic significance in patients with ESCC, and sheds light on the molecular mechanism by which elevated circulating SRGN in cancer patients might promote cancer progression.
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- 2021
47. Targeting SUMO Modification of the Non-Structural Protein 5 of Zika Virus as a Host-Targeting Antiviral Strategy
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Zheng Zhu, Hin Chu, Lei Wen, Shuofeng Yuan, Kenn Ka-Heng Chik, Terrence Tsz-Tai Yuen, Cyril Chik-Yan Yip, Dong Wang, Jie Zhou, Feifei Yin, Dong-Yan Jin, Kin-Hang Kok, Kwok-Yung Yuen, and Jasper Fuk-Woo Chan
- Subjects
antiviral ,flavivirus ,inhibitor ,interferon ,NS5 ,post-translational modification ,SUMO ,Zika ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Post-translational modifications of host or viral proteins are key strategies exploited by viruses to support virus replication and counteract host immune response. SUMOylation is a post-translational modification process mediated by a family of ubiquitin-like proteins called small ubiquitin-like modifier (SUMO) proteins. Multiple sequence alignment of 78 representative flaviviruses showed that most (72/78, 92.3%) have a putative SUMO-interacting motif (SIM) at their non-structural 5 (NS5) protein’s N-terminal domain. The putative SIM was highly conserved among 414 pre-epidemic and epidemic Zika virus (ZIKV) strains, with all of them having a putative SIM core amino acid sequence of VIDL (327/414, 79.0%) or VVDL (87/414, 21.0%). Molecular docking predicted that the hydrophobic SIM core residues bind to the β2 strand of the SUMO-1 protein, and the acidic residues flanking the core strengthen the binding through interactions with the basic surface of the SUMO protein. The SUMO inhibitor 2-D08 significantly reduced replication of flaviviruses and protected cells against ZIKV-induced cytopathic effects in vitro. A SIM-mutated ZIKV NS5 failed to efficiently suppress type I interferon signaling. Overall, these findings may suggest SUMO modification of the viral NS5 protein to be an evolutionarily conserved post-translational modification process among flaviviruses to enhance virus replication and suppress host antiviral response.
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- 2019
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48. Alteration of fecal tryptophan metabolism correlates with shifted microbiota and may be involved in pathogenesis of colorectal cancer
- Author
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Xi-Zhen Sun, Qian-Qian Wang, Dong-Yan Zhao, Geng Qin, Shu-Kun Yao, and Yuan-Chen Zhou
- Subjects
Indoles ,Colorectal adenoma ,Gut flora ,Pathogenesis ,03 medical and health sciences ,chemistry.chemical_compound ,Feces ,0302 clinical medicine ,medicine ,Animals ,Humans ,Tryptophan metabolism ,Kynurenine ,Messenger RNA ,biology ,Microbiota ,Gastroenterology ,Tryptophan ,General Medicine ,Metabolism ,Case Control Study ,medicine.disease ,biology.organism_classification ,Molecular biology ,Colorectal cancer ,Gastrointestinal Microbiome ,chemistry ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Colorectal Neoplasms - Abstract
BACKGROUND Gut tryptophan (Trp) metabolites are produced by microbiota and/or host metabolism. Some of them have been proven to promote or inhibit colorectal cancer (CRC) in vitro and animal models. We hypothesized that there is an alteration of gut Trp metabolism mediated by microbiota and that it might be involved in the pathogenesis of cancer in patients with CRC. AIM To investigate the features of Trp metabolism in CRC and the correlation between fecal Trp metabolites and gut microbiota. METHODS Seventy-nine patients with colorectal neoplastic lesions (33 with colon adenoma and 46 with sporadic CRC) and 38 healthy controls (HCs) meeting the inclusion and exclusion criteria were included in the study. Their demographic and clinical features were collected. Fecal Trp, kynurenine (KYN), and indoles (metabolites of Trp metabolized by gut microbiota) were examined by ultraperformance liquid chromatography coupled to tandem mass spectrometry. Gut barrier marker and indoleamine 2,3-dioxygenase 1 (IDO1) mRNA were analyzed by quantitative real-time polymerase chain reaction. Zonula occludens-1 (ZO-1) protein expression was analyzed by immunohistochemistry. The gut microbiota was detected by 16S ribosomal RNA gene sequencing. Correlations between fecal metabolites and other parameters were examined in all patients. RESULTS The absolute concentration of KYN [1.51 (0.70, 3.46) nmol/g vs 0.81 (0.64, 1.57) nmol/g, P = 0.036] and the ratio of KYN to Trp [7.39 (4.12, 11.72) × 10-3 vs 5.23 (1.86, 7.99) × 10-3, P = 0.032] were increased in the feces of patients with CRC compared to HCs, while the indoles to Trp ratio was decreased [1.34 (0.70, 2.63) vs 2.46 (1.25, 4.10), P = 0.029]. The relative ZO-1 mRNA levels in patients with CRC (0.27 ± 0.24) were significantly lower than those in HCs (1.00 ± 0.31) (P < 0.001), and the relative IDO1 mRNA levels in patients with CRC [1.65 (0.47-2.46)] were increased (P = 0.035). IDO1 mRNA levels were positively associated with the KYN/Trp ratio (r = 0.327, P = 0.003). ZO-1 mRNA and protein levels were positively correlated with the indoles/Trp ratio (P = 0.035 and P = 0.009, respectively). In addition, the genera Asaccharobacter (Actinobacteria) and Parabacteroides (Bacteroidetes), and members of the phylum Firmicutes (Clostridium XlVb, Fusicatenibacter, Anaerofilum, and Anaerostipes) decreased in CRC and exhibited a positive correlation with indoles in all subjects. CONCLUSION Alteration of fecal Trp metabolism mediated by microbiota is associated with intestinal barrier function and tissue Trp metabolism, and may be involved in the pathogenesis of CRC.
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- 2020
49. Stepwise Intercalation-Conversion-Intercalation Sodiation Mechanism in CuInS2 Prompting Sodium Storage Performance
- Author
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Yew Von Lim, Mei Er Pam, Juezhi Yu, Hui Ying Yang, Yang Shang, Yumeng Shi, Xue Liang Li, Dongbin Xiong, Likun Pan, Dong Yan, Shaozhuan Huang, Jian Zhang, Daliang Fang, Ying Bai, and Ye Wang
- Subjects
Materials science ,Renewable Energy, Sustainability and the Environment ,Sodium ,Intercalation (chemistry) ,Energy Engineering and Power Technology ,chemistry.chemical_element ,02 engineering and technology ,Volume change ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Anode ,Fuel Technology ,chemistry ,Chemical engineering ,Chemistry (miscellaneous) ,Materials Chemistry ,sense organs ,0210 nano-technology - Abstract
The low charge storage in intercalation-type reaction and the large volume change induced by conversion/alloying reactions greatly limit the practical selection of anodes in sodium-ion batteries (S...
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- 2020
50. <scp>HbWRKY82</scp> , a novel <scp>IIc WRKY</scp> transcription factor from <scp> Hevea brasiliensis </scp> associated with abiotic stress tolerance and leaf senescence in Arabidopsis
- Author
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Dong Yan, Lifu Yang, Chen Xiaoli, Guijuan Kang, and Rizhong Zeng
- Subjects
0106 biological sciences ,0301 basic medicine ,Senescence ,Physiology ,Abiotic stress ,Cell Biology ,Plant Science ,General Medicine ,Biology ,biology.organism_classification ,01 natural sciences ,WRKY protein domain ,Cell biology ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Arabidopsis ,Genetics ,Transcriptional regulation ,Hevea brasiliensis ,Abscisic acid ,Transcription factor ,010606 plant biology & botany - Abstract
WRKY group transcription factors of model plants and major crops are confirmed to play essential roles in stress responses, senescence, secondary metabolism processes and hormone signal transduction. Previous studies have identified 81 HbWRKY genes from Hevea brasiliensis (the Para rubber tree), but the functions of HbWRKYs in response to abiotic stresses and leaf senescence are unclear. In this study, one novel group IIc WRKY transcription factor named HbWRKY82 was identified and characterized as a stress-associated WRKY in rubber tree. Transient expression and transcriptional activation analyses indicated that HbWRKY82 encoded a nuclear protein and functioned as a transcription activator. The transcription levels of HbWRKY82 were induced by by exogenous Ethrel (ethylene releaser) and abscisic acid (ABA) stimulations, down-regulated in tapping panel dryness rubber trees, and also exhibits significant decrease during the progression of leaf senescence. Overexpression of HbWRKY82 in Arabidopsis improved the tolerance to dehydration and salinity, and decreased the sensitivity to exogenous ABA. Moreover, real-time quantitative PCR analysis demonstrated that HbWRKY82 regulated the transcriptional expression of several stress-responsive genes (DREB1A, ERD10, HKT1, P5CS, RD22, RD29B, SKOR), leaf senescence marker genes (EIN3, WRKY53, NAP), ROS-related genes (RbohD, CSD1, CSD2, FSD3) and hormone signaling genes (EIN3, ABF3, ABF4). Collectively, our findings suggested that HbWRKY82 might function as an important transcriptional regulator in Ethrel -and ABA-mediated leaf senescence and abiotic stress responses, and also be involved in tapping panel dryness, latex flow and regeneration processes of rubber trees via participating in the ET and ROS signaling pathways. This article is protected by copyright. All rights reserved.
- Published
- 2020
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