1. A complement factor H homolog, heparan sulfation, and syndecan maintain inversin compartment boundaries in C. elegans cilia
- Author
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Claire Devine, Karen I. Lange, Zeljka Smit-McBride, Sharon L. Oltjen, Andy Golden, Harold C. Smith, Sofia Tsiropoulou, Oliver E. Blacque, Joanne A Matsubara, Natalie Acker, Andrew Gordus, and Bruce E. Vogel
- Subjects
0303 health sciences ,Multidisciplinary ,Cilium ,Heparan sulfate ,Biology ,eye diseases ,Transmembrane protein ,Syndecan 1 ,Cell biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine.anatomical_structure ,chemistry ,Factor H ,030221 ophthalmology & optometry ,medicine ,Alternative complement pathway ,Compartment (development) ,sense organs ,Neuron ,030304 developmental biology - Abstract
Age-related macular degeneration (AMD) is a leading cause of blindness among the elderly. Canonical disease models suggest that defective interactions between complement factor H (CFH) and cell surface heparan sulfate (HS) result in increased alternative complement pathway activity, cytolytic damage, and tissue inflammation in the retina. Although these factors are thought to contribute to increased disease risk, multiple studies indicate that noncanonical mechanisms that result from defective CFH and HS interaction may contribute to the progression of AMD as well. A total of 60 ciliated sensory neurons in the nematode Caenorhabditis elegans detect chemical, olfactory, mechanical, and thermal cues in the environment. Here, we find that a C. elegans CFH homolog localizes on CEP mechanosensory neuron cilia where it has noncanonical roles in maintaining inversin/NPHP-2 within its namesake proximal compartment and preventing inversin/NPHP-2 accumulation in distal cilia compartments in aging adults. CFH localization and maintenance of inversin/NPHP-2 compartment integrity depend on the HS 3-O sulfotransferase HST-3.1 and the transmembrane proteoglycan syndecan/SDN-1. Defective inversin/NPHP-2 localization in mouse and human photoreceptors with CFH mutations indicates that these functions and interactions may be conserved in vertebrate sensory neurons, suggesting that previously unappreciated defects in cilia structure may contribute to the progressive photoreceptor dysfunction associated with CFH loss-of-function mutations in some AMD patients.
- Published
- 2021