59 results on '"Fatma Yılmaz"'
Search Results
2. Development of Molecularly Imprinted Polymer‐Based Optical Sensor for the Sensitive Penicillin G Detection in Milk
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Volkan Safran, Fatma Yılmaz, Monireh Bakhshpour, Ilgım Göktürk, and Adil Denizli
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Penicillin ,Chemistry ,Colloidal gold ,Nanosensor ,Molecularly imprinted polymer ,medicine ,Nanotechnology ,General Chemistry ,Surface plasmon resonance ,medicine.drug - Published
- 2021
3. Can serum 8-hydroxy-2'-deoxyguanosine levels reflect the severity of pulmonary arterial hypertension?
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Fatma Yılmaz Coskun, Seyithan Taysı, and Meral Kayıkçıoğlu
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Adult ,Male ,Medicine (General) ,medicine.medical_specialty ,Heart disease ,Pulmonary arterial hypertension ,medicine.disease_cause ,Gastroenterology ,World health ,Association ,Pathogenesis ,Young Adult ,chemistry.chemical_compound ,R5-920 ,Internal medicine ,Humans ,Medicine ,Deoxyguanosine ,Familial Primary Pulmonary Hypertension ,8-Hydroxy-2’-deoxyguanosine ,business.industry ,8-Hydroxy-2'-deoxyguanosine ,General Medicine ,Middle Aged ,Brain natriuretic peptide ,medicine.disease ,Dna-Damage ,chemistry ,Oxidative stress ,Hypertension ,Biomarker (medicine) ,Female ,business - Abstract
OBJECTIVE: Oxidative stress plays a pivotal role in the pathogenesis of pulmonary arterial hypertension. 8-Hydroxy-2'-deoxyguanosine is a sensitive biomarker that reflects the degree of oxidative damage to DNA. We investigated whether serum 8-Hydroxy-2'-deoxyguanosine is a clinically useful biomarker for the severity of pulmonary arterial hypertension. METHODS: We measured serum 8-Hydroxy-2'-deoxyguanosine levels in 25 patients (age 37 +/- 13 years, 68% women) diagnosed with idiopathic pulmonary arterial hypertension, familial pulmonary arterial hypertension, or pulmonary arterial hypertension associated with congenital heart disease. The severity of pulmonary arterial hypertension was evaluated by six-min walking distance, World Health Organization functional class, and serum brain natriuretic peptide levels. Age and gender-matched 22 healthy subjects served as the control group. RESULTS: The comparison of 8-Hydroxy-2'-deoxyguanosine levels between patients and controls was not statistically different [(19.86 +/- 9.79) versus (18.80 +/- 3.94) ng/mL, p=0.622)]. However, there was a significant negative correlation between 8-Hydroxy-2'-deoxyguanosine levels and six-min walking distance (r= -0.614, p=0.001). Additionally, serum 8-Hydroxy-2'-deoxyguanosine levels in patients with functional class III-IV were significantly higher than those with functional class I-II (functional class III-IV 32.31 +/- 10.63 ng/mL versus functional class I-II 16.74 +/- 6.81 ng/mL, respectively, p=0.003). CONCLUSION: The 8-Hydroxy-2'-deoxyguanosine levels were significantly correlated with exercise capacity (six-min walking distance) and symptomatic status (functional class), both of which show the severity of pulmonary arterial hypertension in patients.
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- 2021
4. Evaluation of the subfoveal choroidal and outer retinal layer thickness in obese women
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Mehtap Çağlayan, Mehmet Fuat Alakus, Umut Dag, Kübra Metin, Eşref Araç, Fatma Yılmaz Öncül, and Hasan Öncül
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medicine.medical_specialty ,Retinal Pigment Epithelium ,Retina ,03 medical and health sciences ,chemistry.chemical_compound ,Health problems ,0302 clinical medicine ,Optical coherence tomography ,Ophthalmology ,medicine ,Humans ,medicine.diagnostic_test ,Choroid ,business.industry ,Retinal ,Layer thickness ,Obesity, Morbid ,chemistry ,030221 ophthalmology & optometry ,Retinal imaging ,Female ,business ,Tomography, Optical Coherence ,030217 neurology & neurosurgery ,Optometry - Abstract
Obesity has become one of the most important health problems of today with its increasing prevalence. With the development of retinal imaging systems, obesity has been observed to be associated with changes in outer retinal layers and choroid in women.The aim of this study was to examine retinal layer parameters and their relationship with body mass index in obese women.The study included 197 eyes of 197 women, of whom 44 were normal-weight, 40 were overweight, 40 were class 1 obese, 38 were class 2 obese, and 35 were morbidly obese. The thickness of the choroid was measured manually using an enhanced-depth imaging optical coherence tomography scanning program.The mean choroidal thickness values in five locations were lower than those of normal-weight, overweight, class 1, and class 2 obese women, in morbidly obese women (p 0.05 for all). Subfoveal outer retinal layer thickness and specific sublayer thickness of the photoreceptor layer values were significantly lower in morbidly obese women than in normal-weight women, but there were no statistically significant differences between the groups in retinal pigment epithelium thickness and Bruch's membrane thickness (p = 0.001, p 0.001, p = 1.00, and p = 0.101, respectively). Furthermore, there were significant negative relationships between body mass index and subfoveal choroidal thickness, subfoveal outer retinal layer thickness, and specific sublayer thickness of the photoreceptor layer values (r = -0.327, p 0.001; r = -0.259, p 0.001; and r = -0.281, p 0.001, respectively).Morbid obesity was associated with a thinner choroid, subfoveal outer retinal layer, and specific sublayer thickness of the photoreceptor layer in women. Furthermore, retinal hypoxia associated with morbid obesity may be related to a decrease in photoreceptor layer thickness. Thinning of the outer retinal layer may also lead to atrophy of the cone sheath.
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- 2021
5. Selective dopamine detection by SPR sensor signal amplification using gold nanoparticles
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Monireh Bakhshpour, Fatma Yılmaz, Deniz Türkmen, Adil Denizli, Suleyman Asir, and Ilgım Göktürk
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Contact angle ,Aqueous solution ,Polymerization ,Chemistry ,Colloidal gold ,Materials Chemistry ,Nanoparticle ,General Chemistry ,Repeatability ,Surface plasmon resonance ,Molecular imprinting ,Catalysis ,Nuclear chemistry - Abstract
In this study, selective and sensitive detection of the neurotransmitter dopamine (DA) in both aqueous solution and biological samples was performed using a surface plasmon resonance (SPR) sensor based on a molecular imprinting technique. For this, dopamine-imprinted poly(2-hydroxyethyl methacrylate-N-methacroyl-(L)-cysteine methyl ester-gold nanoparticles-N-methacryloyl-L-phenylalanine methyl ester) [PHEMAC-AuNPs/MAPADA] nanoparticles were prepared. Furthermore, to evaluate the imprinting efficiency, non-imprinted [PHEMAC-AuNPs/MAPA] nanoparticles were designed using the same polymerization procedure except for the addition of dopamine molecules. To examine the effect of incorporating AuNPs to increase the SPR signal response, control experiments were carried out via the SPR biosensor produced using [PHEMAC/MAPADA] nanoparticles prepared without the addition of AuNPs. Characterization studies of dopamine- + imprinted [PHEMAC-AuNPs/MAPADA] and non-imprinted [PHEMAC-AuNPs/MAPA] nanoparticles was performed with a zetasizer and an FTIR-ATR spectrophotometer. In addition, dopamine-imprinted [PHEMAC-AuNPs/MAPADA] and non-imprinted [PHEMAC-AuNPs/MAPADA] SPR sensors were characterized by ellipsometer and contact angle measurements. The high imprinting efficiency (I.F: 9.67) of the dopamine-imprinted [PHEMAC-AuNPs/MAPADA] SPR sensor was determined by comparing it with the non-imprinted [PHEMAC-AuNPs/MAPA] SPR sensor. A good linear relationship was obtained in the 0.01–0.5 ppb concentration range with correlation coefficients of 0.9818 and 0.9819, respectively. The dopamine-imprinted [PHEMAC-AuNPs/MAPADA] SPR sensor was 5.53 and 4.59 times more selective for the target molecule dopamine than for epinephrine (EP) and norepinephrine (NE), respectively. The repeatability of the [PHEMAC-AuNPs/MAPADA] SPR sensor was assessed with a 0.5 ppb dopamine solution, with the percent relative standard deviation of the intra-assays (RSD) being less than 1.7%, indicating negligible loss of dopamine sensing capability after four adsorption–desorption cycles with the same sensor.
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- 2021
6. Electrochromatographic separation of hydrophobic amino acid enantiomers by molecularly imprinted capillary columns
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Fatma Yılmaz, Adil Denizli, Ilgım Göktürk, Suleyman Asir, Koray Şarkaya, and Handan Yavuz
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0106 biological sciences ,chemistry.chemical_classification ,0303 health sciences ,Capillary electrochromatography ,Chromatography ,Capillary action ,Tryptophan ,Bioengineering ,Phenylalanine ,01 natural sciences ,Applied Microbiology and Biotechnology ,Biochemistry ,Amino acid ,03 medical and health sciences ,chemistry.chemical_compound ,Monomer ,chemistry ,Polymerization ,010608 biotechnology ,Enantiomer ,030304 developmental biology - Abstract
In the present study, the enantiomeric forms of hydrophobic amino acids ( l -tryptophan, l -tyrosine, and l -phenylalanine) were separated by molecularly imprinted capillary columns (MICC) via Capillary Electrochromatography (CEC) for the first time. The monomer ratio, crosslinker ratio, template molecule ratio, the porogen ratio and type, polymerization time, and also the effect of temperature were examined to increase the permeability properties of MICC. FTIR, SEM and BET analyses were realized for the characterization of MICC. The effect of the electric field, organic solvent ratio, and pressure were carried out experimentally to determine the optimum conditions. The separation performances of MICC and the non-imprinted capillary columns (NICC) were compared electrochromatographically.
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- 2020
7. Separation of histidine enantiomers by capillary electrochromatography with molecularly imprinted monolithic columns
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Koray Şarkaya, Sisem Ektirici, Ilgım Göktürk, Handan Yavuz, Adil Denizli, Suleyman Asir, and Fatma Yılmaz
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Capillary electrochromatography ,Chromatography ,Chemistry ,Enantiomer ,Molecular imprinting ,Histidine - Published
- 2020
8. S-citalopram imprinted monolithic columns for capillary electrochromatography enantioseparations
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Adil Denizli, Fatma Yılmaz, Ilgım Göktürk, and Ali Derazshamshir
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Capillary electrochromatography ,Chromatography ,Monolithic HPLC column ,Chemistry ,Clinical Biochemistry ,Reproducibility of Results ,Stereoisomerism ,Citalopram ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,Molecular Imprinting ,Electrophoresis ,Capillary electrophoresis ,Capillary Electrochromatography ,NIP ,Enantiomer ,Molecular imprinting - Abstract
In this study, the molecular imprinting method was used to separate enantiomeric forms of chiral antidepressant drug, R,S-citalopram (R,S-CIT) in aqueous solution by CEC system combining the advantages of capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). For that, an amino acid based molecularly imprinted monolithic capillary column was designed and used as a stationary phase for selective separation of S-citalopram (S-CIT) for the first time. S-CIT was selectively separated from aqueous solution containing the other enantiomeric form of R-CIT, which is same in size and shape to the template molecule. Morphology of the molecularly imprinted (MIP S-CIT) and non-imprinted (NIP S-CIT) monolithic capillary columns was observed by scanning electron microscopy. Imprinting efficiency of MIP S-CIT monolithic capillary column used for selective S-CIT separation was verified by comparing with NIP S-CIT and calculated imprinting factor (I.F:1.81) proved the high selectivity of the MIP S-CIT for S-CIT. Cavities formed for S-CIT form enabled selective (α = 2.08) separation of the target molecule from the other enantiomeric R-CIT form. Separation was achieved in a short period of 10 minutes, with the electrophoretic mobility of 7.68 × 10-6 m2 /Vs for R,S-CIT at pH 7.0 10 mM PB and 50% ACN ratio. The performance of both MIP S-CIT and NIP S-CIT columns was estimated by repeating the R,S-CIT separations with intra-batch and inter-batch studies for reproducibility of retention times of R.S-CITs. Estimated RSD values that are lower than 2% suggest that the monolithic columns separate R,S-CIT enantiomers without losing separation efficiency. This article is protected by copyright. All rights reserved.
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- 2021
9. Protein depletion with bacterial cellulose nanofibers
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Adil Denizli, Fatma Yılmaz, Ilgım Göktürk, Emel Tamahkar, and Hitit Üniversitesi, Mühendislik Fakültesi, Kimya Mühendisliği Bölümü
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Proteomics ,Clinical Biochemistry ,Nanofibers ,Serum Albumin, Human ,02 engineering and technology ,Models, Biological ,01 natural sciences ,Biochemistry ,Metal Ion Coordination ,Analytical Chemistry ,Molecular Imprinting ,Bacterial Cellulose Nanofibers ,chemistry.chemical_compound ,Adsorption ,Molecularly Imprinted Polymers ,Equipment Reuse ,medicine ,Humans ,Cellulose ,Aqueous solution ,Chromatography ,Bacteria ,010401 analytical chemistry ,Molecularly imprinted polymer ,Proteins ,Cell Biology ,General Medicine ,021001 nanoscience & nanotechnology ,Human serum albumin ,0104 chemical sciences ,body regions ,Monomer ,chemistry ,Bacterial cellulose ,embryonic structures ,Protein Recognition ,0210 nano-technology ,Molecular imprinting ,Selectivity ,Nuclear chemistry ,medicine.drug - Abstract
In this study, we have reported a novel fabrication technique for human serum albumin (HSA) imprinted composite bacterial cellulose nanofibers (MIP-cBCNFs) used for the depletion of HSA selectively from artificial blood plasma for proteomic applications. Molecular imprinting was achieved by using metal ion coordination interactions of N?methacryloyl?(L)?histidinemethylester (MAH) monomer and Cu(II) ions. MAH-Cu(II)-HSA complex was polymerized with bacterial cellulose nanofibers (BCNFs) under constant stirring at room temperature. The characterization of the MIP-cBCNFs was carried out by FTIR-ATR, SEM, contact angle measurements and surface area measurements. The adsorption experiments of HSA onto the MIP-BCNFs and NIP-BCNFs from aqueous HSA solutions were investigated in a batch system. The selectivity of the MIP-cBCNFs was investigated by using non-template human transferrin (HTR), and myoglobin (Myo). The relative selectivity coefficients of the MIP-cBCNFs were calculated as 4.73 and 3.02 for HSA/HTR and HSA/Myo molecules, respectively. In addition, the depletion of HSA from artificial human plasma was confirmed by SDS-PAGE and 2-D gel electrophoresis. As a result, it has been shown that metal ion coordination interactions contribute to specific binding of template when preparing MIP-cBCNFs for the depletion of HSA with a high adsorption capacity, significant selectivity and reusability. © 2018
- Published
- 2018
10. Sensitive and selective detection of amitrole based on molecularly imprinted nanosensor
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Fatma Yılmaz, Zübeyde Baysal, Oguz Cakir, Ilgım Göktürk, Monireh Bakhshpour, Dicle Üniversitesi Bilim ve Teknoloji Uygulama ve Araştırma Merkezi, Çakır, Oğuz, and Baysal, Zübeyde
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chemistry.chemical_classification ,Molecular imprinting ,Surface Properties ,education ,High selectivity ,Biosensing Techniques ,Surface Plasmon Resonance ,Nanofilm ,Combinatorial chemistry ,Amino acid ,Molecular Imprinting ,Functional monomer ,chemistry ,Structural Biology ,Nanosensor ,Limit of Detection ,Surface plasmon resonance ,Nanoparticles ,Molecular Biology ,Amitrole ,Sensor - Abstract
SPR sensor used for amitrole detection was prepared without using any modification. Molecularly imprinted SPR sensor enabled high selectivity for amitrole pesticide. Amino acid-based functional monomer MATrp was integrated as a recognition element. Tailor-made SPR sensor enables real-time monitoring of amitrole pesticide. Synthetic recognition sites provided by MATrp were prepared without labeling.Herein, a surface plasmon resonance (SPR)-based nanosensor was fabricated using molecular imprinting technique for the selective and sensitive detection of amitrole, a commonly used highly toxic pesticide. For this purpose, polymeric nanofilms involving N-methacryloyl-l-tryptophan methyl ester and ethylene glycol dimethacrylate which are functional monomer and cross-linker, respectively, were prepared on the SPR chips surface via the ultravoilet polymerization method. The nanofilms were characterized by several techniques such as ellipsometry, Fourier transform infrared-attenuated total reflection, atomic force microscope and contact angle measurements. The kinetic analyses for amitrole detection were examined through amitrole-imprinted and non-imprinted SPR sensor chips. The developed imprinted sensor showed high selectivity to the amitrole molecule than similar molecules and a good linear relationship for the 0.06-11.90 nM concentration range with a low limit of detection value of 0.037 nM. The high imprinting efficiency (I.F: 62.38) of the amitrole-imprinted SPR sensor was determined by comparing it with the non-imprinted SPR sensor. The most suitable model of this sensor is the Langmuir adsorption model. To statistically assess the reusability of the sensor, intraday experiments were tested three times with five replicates. The relative standard deviation% value less than1.5 indicates high reproducibility for both sensor production and reproducibility of the method. The prepared sensor was also applied efficiently for the selective detection of the amitrole in spiked samples prepared from vegetables to evaluate the matrix effect. As a result, amitrole-imprinted SPR sensors have been estimated to be highly selective, fast responsive, easy to use, reusable and sensitive in detecting amitrole in both natural source and aqueous solutions. Label-free amitrole detection was performed by an aminoacid-based SPR sensor fabricated without the need for complex coupling processes.
- Published
- 2021
11. Selective recognition of nucleosides by boronate affinity organic-inorganic hybrid monolithic column
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Fatma Yılmaz, Sisem Ektirici, Ilgım Göktürk, Adil Denizli, BAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümü, and Yılmaz, Fatma
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inorganic chemicals ,Monolithic HPLC column ,Boronate Affinity Chromatography ,Clinical Biochemistry ,030226 pharmacology & pharmacy ,01 natural sciences ,Biochemistry ,High-performance liquid chromatography ,Chromatography, Affinity ,Analytical Chemistry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Adsorption ,Humans ,Molecule ,Organosilicon Compounds ,Hybrid Monolithic Column ,Chromatography, High Pressure Liquid ,Chromatography ,010401 analytical chemistry ,Methacryloyl-L-histidine ,Nucleosides ,Cell Biology ,General Medicine ,Boronic Acids ,Combinatorial chemistry ,0104 chemical sciences ,Monomer ,chemistry ,Selective adsorption ,Polyhedral Oligomeric Silsesquioxane ,Selectivity ,Nucleoside - Abstract
WOS:000612823000013 PubMed: 33383498 Boronic acids are important ligands used to selectively recognize and enrich compounds containing cis-diol groups such as nucleosides. In the present study, organic-inorganic hybrid [POSS-MAH-BPA] monolithic column was prepared for the first time in the literature as a new boronate affinity system for the recognition of nucleosides. The selectivity of the [POSS-MAH-PBA] boronate affinity monolithic column for the recognition of cis-diol containing adenosine nucleoside from its analogue molecule of deoxyadenosine triphosphate, dATP, noncis-diol containing compound was investigated both by UV and HPLC studies. When the relative selectivity coefficients are compared, the [POSS-MAH-PBA] boronate affinity monolithic column is 4.25 times more selective for adenosine than [POSS-MAH] monolithic column. Besides, to determine endogenous nucleosides in biological fluids, which may serve as non-invasive cancer biomarkers, nucleosides were spiked into the urine solutions and passed through the [POSS-MAH-PBA] boronate affinity monolithic column, and the nucleosides were confirmed by HPLC. The adenosine recognition capability of the [POSS-MAH-PBA] boronate affinity monolithic column with an average enrichment factor of 48.9-fold was apparently superior to that of the [POSSMAH] monolithic column. Methacryl Polyhedral Oligomeric Silsesquioxanes (POSS-MA) with nano-sized stable 3-dimensional architectures provided the advantage of being used as an adsorbent for the monolithic structure by providing high surface area, 507.60 m(2)/g, and enabling vinyl groups to function with amino acid-based MAH monomers capable of providing electrons to coordinate PBA. Recovery results of more than 90% for adenosine showed that the [POSS-MAH-PBA] boronate affinity monolithic column could be a promising adsorbent for selective adsorption of cis-diol containing compounds such as nucleosides.
- Published
- 2021
12. Chiral Separations by Capillary Electrophoresis and related Techniques with Different Chiral Selectors: A Review
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Fatma Yılmaz, Koray Şarkaya, Ilgım Göktürk, and Adil Denizli
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Chromatography ,Capillary electrophoresis ,Engineering ,Chemistry ,Mühendislik ,Kapiler Elektroforez,kiral selektörler,kiral katkı maddeleri,kiral ayırım - Abstract
Recognition mechanism and enantiomerically separations of the chiral compounds are subjects that always stimulate the great interest of researchers in pharmacology and natural sciences, who are interested in finding solutions for both analytical purity and preparative purposes. Capillary Electrophoresis has become one of the most important analytical approaches for enantiomeric separations due to its superior properties, such as high resolution and high efficiency of chiral selectors. In this field, where researchers continue to be interested, the distinctions continue to develop day by day, with the introduction of new techniques developed on the basis of Capillary Electrophoresis philosophy in parallel with the development process of technology, as well as the chiral selectors of many different forms. In this review, besides some descriptive theoretical information about capillary electrophoresis and the techniques associated with it, studies on chiral separations using different chiral selectors or different chiral additives, such as molecularly imprinted polymers, cyclodextrins, Metal-organic frameworks, ionic liquids, nanoparticles and monoliths in the last nearly 10 years (2010-2020) were examined.
- Published
- 2020
13. Evaluation of Dirui® H13-Cr and H12-800MA strips for albuminuria detection
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Fatma Yılmaz, Emiş Deniz Akbulut, and Müjgan Ercan
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Screening test ,albumin to creatinine ratio ,lcsh:Medicine ,Urine ,STRIPS ,Urine albumin ,law.invention ,chemistry.chemical_compound ,law ,strip ,medicine ,Immunoturbidimetry ,Creatinine ,lcsh:R5-920 ,Chromatography ,business.industry ,screening ,lcsh:R ,Albumin ,sensitivity ,Albumin excretion ,chemistry ,Albuminuria ,medicine.symptom ,business ,lcsh:Medicine (General) - Abstract
Increased urine albumin excretion is suggested to be an early marker of glomerular damage. Evaluation of a tests performance characteristics such as diagnostic sensitivity and specifictiy is a necessity before application into routine practice. To our knowledge up to now there is no study evaluating Dirui test strips for albuminuria determination. Therefore the objective of this study was to test these strips for agreement with the established method used in our routine laboratory. Freshly collected 177 urine specimens were included in the study. Strips were read on instruments Dirui H-500 and Dirui H-800 using reflectance photometry. For DiruiH13-Cr strip albumin to creatinine ratio (ACR) and for DiruiH12-800MA strip albumin determinations were compared with the analyses performed using immunoturbidimetry and kinetic Jaffe method. Sensitivity values for estimated ACR using DiruiH13-Cr strip and albumin concentration using DiruiH12-800MA strip were calculated as 80%, 72%, respectively. Specificity values were as; 71% for Dirui H13-Cr strip and 78% for Dirui H12-800MA strip. Screening tests using urine test strips should be validated before acceptance in the routine use. Dirui strips evaluated in this study seem to have a lower sensitivity than suggested and excess number of false negatives seems to limit utility of these strips. [Med-Science 2018; 7(3.000): 532-5]
- Published
- 2018
14. Separation of D,L-Ampicillin by ligand exchange-micellar electrokinetic chromatography
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Suleyman Asir, Duygu Sari, Fatma Yılmaz, Ilgım Göktürk, Ali Derazshamshir, Adil Denizli, Kimya, BAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümü, and Yılmaz, Fatma
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Chromatography ,Ligand ,Chemistry ,Biochemistry ,Micelle ,Micellar electrokinetic chromatography ,Capillary Electrophoresis ,Capillary electrophoresis ,Ampicillin ,medicine ,Molecular Medicine ,Molecular Biology ,Biotechnology ,medicine.drug - Abstract
WOS:000503065300011 In this study, D,L-ampicillin separation was carried out by ligand exchange-micellar electrokinetic chromatography method using L-Lysine monohydrochloride as a ligand and copper (II) sulfate pentahydrate is a central ion supplier. Isomeric separations were performed using capillary electrophoresis (CE) instrument, in which SDS-L-Lys-Cu+2 micelle complex was used as a pseudostationary phase. The effect of pH, SDS amount, applied electrical field, pressure, organic solvent ratio and ampicillin D,L-ratios were investigated. Fast,inexpensive and sensitive approach for the simultaneous separation of D,L-ampicillin in both aqueous and real antibiotic sample was performed using CE coupled with UV detector. The separation was achieved in a short period of 7 minutes with high-sensitivity andlow-detection limit of 1.25 mu M by the developed SDS-L-Lys-Cu+2 micelle-chiral selector complexes without using any extra process such as imprinting or spacer arms for the immobilization of the ligands.
- Published
- 2019
15. Oxidative stress level in patients with chronic kidney disease
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Ezgi Coskun Yenigun, Fatih Dede, Ihsan Ates, Nihal Ozkayar, Fatma Yılmaz, Özcan Erel, Nergiz Bayrakci, and Salim Neselioglu
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Psychiatry and Mental health ,Neuropsychology and Physiological Psychology ,Chemistry ,Thiol oxidation ,General Medicine ,Molecular biology - Abstract
Amac: Bu calismada kronik bobrek hastaligi olan hastalarda dinamik tiyol/disulfid dengesinin arastirilmasi amaclandi. Gerec ve Yontem: Calismaya evre 1-5 (non diyaliz) 150 ( 57 erkek, 93 kadin) kronik bobrek hastasi ve (76 ( 30 erkek, 46 kadin ) saglikli kontrol grubu alindi. Kan tiyol/disulfid dengesi yeni gelistirilen otomatik ve kalorimetrik bir yontemle olculdu. Bulgular: Tum populasyonun serum thiol duzeyi ortalamasi 320.8±57.8 µmol/L , total thiol duzeyi ortalamasi 352.0±61.5 µmol/L , ortalama disulfid duzeyi 15.6±5.3 µmol/L idi. Kronik bobrek hastalarinda ortalama serum thiol ( p
- Published
- 2018
16. Rapid sensing of Cu+2 in water and biological samples by sensitive molecularly imprinted based plasmonic biosensor
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Necdet Sağlam, Fatma Yılmaz, Ilgım Göktürk, Adil Denizli, Ali Derazshamshir, and Volkan Safran
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Aqueous solution ,Chemistry ,010401 analytical chemistry ,Langmuir adsorption model ,Nanoparticle ,02 engineering and technology ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,symbols.namesake ,Adsorption ,Monolayer ,symbols ,Molecule ,0210 nano-technology ,Selectivity ,Biosensor ,Spectroscopy ,Nuclear chemistry - Abstract
In this study, copper (II) ion (Cu+2) imprinted poly(hydroxyethyl methacrylate-N-metacryloyl-(L)-cysteine methyl ester [PHEMAC-Cu+2] nanoparticles were synthesized by two-phase mini-emulsion polymerization method and applied to the SPR sensor chip surface for the selective determination of the Cu+2 ions in both aqueous solution, Cu+2-spiked artificial urine and physiological serum samples to investigate the effects of metabolite residues during the analysis. The non-imprinted [PHEMAC] nanoparticles were synthesized by applying the same procedure for the [PHEMAC-Cu+2] nanoparticle synthesis except the addition of Cu+2 ions as a control experiment to evaluate the selectivity of the [PHEMAC-Cu+2] nanoparticles. Roughness differences between [PHEMAC-Cu+2] and [PHEMAC] nanoparticles showed that the imprinting process of Cu+2 ions was performed successfully. [PHEMAC-Cu+2] and [PHEMAC] SPR biosensors prepared by attaching nanoparticles onto the surface of sensor chips, were characterized by atomic force microscope, ellipsometer, contact angle measurements. Langmuir adsorption model was found the most applicable model for this affinity system. Results showed that Cu+2 affinity regions on the surface of [PHEMAC-Cu+2] SPR biosensor were homogeneously distributed and have a monolayer structure. Having the high imprinting efficiency with the imprinting factor of 4.74, the [PHEMAC-Cu+2] SPR biosensor was found to show more selectivity towards the target Cu+2 than the non-imprinted [PHEMAC] SPR biosensor. The selectivity studies of [PHEMAC-Cu+2] SPR biosensors for Cu+2 detection were investigated by using Zn+2 and Ni+2 solutions selected as competitor molecules. The results of intraday and interday precision studies were carried out to ascertain the reproducibility of the proposed method and reported as percent relative standard deviation (%RSD) value.
- Published
- 2019
17. Phenol removal from wastewater by surface imprinted bacterial cellulose nanofibres
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Fatma Yılmaz, Adil Denizli, Necdet Sağlam, Ilgım Göktürk, Emel Tamahkar, Ali Derazshamshir, and [Belirlenecek]
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Turkey ,Polymers ,0208 environmental biotechnology ,Nanofibers ,02 engineering and technology ,010501 environmental sciences ,Wastewater ,bacterial cellulose nanofibres ,01 natural sciences ,Molecular Imprinting ,chemistry.chemical_compound ,symbols.namesake ,Adsorption ,Phenols ,Desorption ,Environmental Chemistry ,Phenol ,Cellulose ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Water Science and Technology ,Molecularly imprinted polymer ,Langmuir adsorption model ,General Medicine ,020801 environmental engineering ,Monomer ,chemistry ,Chemical engineering ,Polymerization ,surface imprinting ,phenol removal ,Bacterial cellulose ,Molecularly imprinted polymers ,symbols - Abstract
In this study, we have reported a novel wastewater treatment technique by phenol imprinted bacterial cellulose (BC-MIP) nanofibres with high specificity and adsorption capacity. N-methacryloyl-(L) phenylalanine methyl ester (MAPA) functional monomer was used to create specific binding sites for the template molecule phenol via electrostatic and hydrophobic interactions. BC-MIP nanofibres were synthesized by surface imprinting approach in the presence of different amounts of total monomer (% weight), monomer/template ratio and polymerization time. Then, the nanofibres were characterized by FTIR-ATR, surface area analysis (BET), elemental analysis, scanning electron microscopy (SEM) and contact angle measurements. Adsorption studies were performed with respect to pH, temperature and ionic strength, and the adsorption capacity was calculated by using the spectrophotometer. In order to desorb the adsorbed phenol from BC-MIP nanofibres, 0.1 M NaCl solution was used. Besides, BC-MIP nanofibres were applied to real wastewater samples from Ergene basin in Turkey. The suitable equilibrium isotherm was determined as Langmuir isotherm. To evaluate the selectivity of the BC-MIP nanofibres, similar molecules were utilized as competitor molecules, which were 2-chlorophenol, 4-chlorophenol and 2,4-dichlorophenol. Electrostatic interactions were found to contribute to the generation of specific recognition binding sites. The results have shown that imprinting of phenol was achieved successfully with high adsorption capacity. The phenol removal efficiency was reported up to 97%. BC-MIP nanofibres were used 10 times with a negligible decrease in adsorption capacity. [GRAPHICS] . WOS:000471539100001 2-s2.0-85065257535 PubMed: 30919740
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- 2019
18. Real time monitoring and label free creatinine detection with artificial receptors
- Author
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Nilay Bereli, Aykut Arif Topçu, Adil Denizli, Erdoğan Özgür, Fatma Yılmaz, [Topcu, Aykut Arif] Aksaray Univ, Dept Chem, Aksaray, Turkey -- [Ozgur, Erdogan] Hacettepe Univ, Adv Technol Applicat & Res Ctr, Ankara, Turkey -- [Yilmaz, Fatma] Abant Izzet Baysal Univ, Dept Chem Technol, Bolu, Turkey -- [Bereli, Nilay -- Denizli, Adil] Hacettepe Univ, Dept Chem, Ankara, Turkey, and Fen-Edebiyat Fakültesi
- Subjects
Materials science ,Molecular imprinting ,education ,02 engineering and technology ,Artificial receptor ,01 natural sciences ,Contact angle ,chemistry.chemical_compound ,Ellipsometry ,Surface plasmon resonance ,General Materials Science ,Receptor ,Detection limit ,Creatinine ,Chromatography ,Mechanical Engineering ,010401 analytical chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Linear range ,chemistry ,Mechanics of Materials ,0210 nano-technology - Abstract
WOS: 000471359400002, Molecular imprinting technique is used to design artificial creatinine receptor on the gold surface of surface plasmon resonance (SPR) chip using N-methacryloyl-(L)-histidine methyl ester (MAH), as a functional monomer. Surface characterization of SPR sensor chip is performed with atomic force microscope (AFM), ellipsometry and contact angle measurements. Creatinine imprinted SPR sensor is characterized with a linear range between 1 and 100 mM and limit of detection (LOD) and limit of quantification (LOQ) of creatinine are found 57 mu M and 190 mu M, respectively. N-hydroxysuccinimide (NHS) and creatine molecules are selected to examine the selectivity of creatinine imprinted SPR sensor. Reusability studies of SPR sensor is determined in urine mimic samples., Republic of Turkey, Ministry of Science, Industry and Technology [0505_TGSD.2015_2], This study was funded and supported by Republic of Turkey, Ministry of Science, Industry and Technology (Grand No. 0505_TGSD.2015_2).
- Published
- 2019
19. Quartz crystal microbalance based biosensors for detecting highly metastatic breast cancer cells via their transferrin receptors
- Author
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Seda Atay, Adil Denizli, Canan Çakır, Kevser Piskin, Fatma Yılmaz, and Handan Yavuz
- Subjects
chemistry.chemical_classification ,Chromatography ,General Chemical Engineering ,Ethylene glycol dimethacrylate ,010401 analytical chemistry ,technology, industry, and agriculture ,General Engineering ,Transferrin receptor ,02 engineering and technology ,(Hydroxyethyl)methacrylate ,Quartz crystal microbalance ,021001 nanoscience & nanotechnology ,01 natural sciences ,Receptor–ligand kinetics ,0104 chemical sciences ,Analytical Chemistry ,chemistry.chemical_compound ,chemistry ,Transferrin ,Monolayer ,0210 nano-technology ,Biosensor - Abstract
A quartz crystal microbalance (QCM) biosensor was developed to detect highly metastatic breast cancer cells by functionalizing the gold sensor surface with transferrin attachment. MDA-MB 231 breast cancer cells with high and MCF 7 cells with low metastatic potential and transferrin expression were used. Serum starved MDA-MB-231 cells were used as control cells. First, poly(2-hydroxyethyl methacrylate) (PHEMA) nanoparticles were prepared by mini-emulsion polymerization of hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate (EGDMA). Nanoparticles were characterized with a zeta-sizer and then their suspension is dropped on the surface of the QCM and the dried QCM surface was modified further by activation with carbodiimide and transferrin attachment. The QCM biosensor was analyzed by using atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry (FTIR) and contact angle measurements. The cells were applied to the derivatized QCM sensor to investigate the affinity and binding kinetics. The nanoparticles and transferrin were found to form a monolayer on the QCM surface. Binding kinetics were best fitted to the Langmuir–Freundlich adsorption model. The QCM signal was correlated well with the number of transferrin receptors on cells. It is concluded that the QCM biosensor functioning via transferrin receptor interactions may be used to detect breast cancer cells with high metastatic potential.
- Published
- 2016
20. Dye affinity cryogels for plasmid DNA purification
- Author
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Duygu Çimen, Deniz Türkmen, Adil Denizli, Işık Perçin, and Fatma Yılmaz
- Subjects
DNA, Bacterial ,chemistry.chemical_classification ,Materials science ,Chromatography ,Triazines ,Ligand ,Radical polymerization ,Bioengineering ,Polymer ,(Hydroxyethyl)methacrylate ,Redox ,Biomaterials ,chemistry.chemical_compound ,Adsorption ,chemistry ,Mechanics of Materials ,Diamine ,Escherichia coli ,Ammonium persulfate ,Cryogels ,Polyhydroxyethyl Methacrylate ,Plasmids - Abstract
The aim of this study is to prepare megaporous dye-affinity cryogel discs for the purification of plasmid DNA (pDNA) from bacterial lysate. Poly(hydroxyethyl methacrylate) [PHEMA] cryogel discs were produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium persulfate (APS) redox pair in an ice bath. Cibacron Blue F3GA was used as an affinity ligand (loading amount: 68.9μmol/g polymer). The amount of pDNA adsorbed onto the PHEMA-Cibacron Blue F3GA cryogel discs first increased and then reached a plateau value (i.e., 32.5mg/g cryogel) at 3.0mg/mL pDNA concentration. Compared with the PHEMA cryogel (0.11mg/g cryogel), the pDNA adsorption capacity of the PHEMA-Cibacron Blue F3GA cryogel (32.4mg/g polymer) was improved significantly due to the Cibacron Blue 3GA immobilization onto the polymeric matrix. pDNA adsorption amount decreased from 11.7mg/g to 1.1mg/g with the increasing of NaCl concentration. The maximum pDNA adsorption was achieved at 4°C. The overall recovery of pDNA was calculated as 90%. The PHEMA-Cibacron Blue F3GA cryogel discs could be used five times without decreasing the pDNA adsorption capacity significantly. The results show that the PHEMA-Cibacron Blue F3GA cryogel discs promise high selectivity for pDNA.
- Published
- 2015
21. Impact of vitamin D supplementation on the ocular surface in patients with vitamin d deficiency
- Author
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Hasan Öncül, Mehtap Çağlayan, Mehmet Fuat Alakus, Fatma Yılmaz Öncül, Umut Dag, and Eşref Araç
- Subjects
medicine.medical_specialty ,Vitamin d supplementation ,business.industry ,General Medicine ,medicine.disease ,Gastroenterology ,eye diseases ,vitamin D deficiency ,chemistry.chemical_compound ,chemistry ,Internal medicine ,medicine ,Vitamin D and neurology ,Tears ,In patient ,Ocular Surface Disease Index ,business ,Cholecalciferol ,Ocular surface - Abstract
Aim: The aim of this study was to determine the effect of vitamin D (vit D) supplementation on the ocular surface parameters in patients with vit D deficiency.Material and Methods: In total, 63 patients with serum vit D deficiency were included. Patients were treated with an oral dose of 300.000 IU cholecalciferol. The ocular surface disease index (OSDI) questionnaire, fluorescein tears breakup time (FBUT), Oxford score, and Schirmer’s 1 test were performed for all patients. The data was obtained initially and at 4, 8, and 12 weeks after vit D supplementation.Results: Forty-nine of the patients were female and 14 were male. The mean age was 30.8±9.7 (18–58) years. All 63 patients came to the first follow-up, 57 patients came to the second follow-up, and 48 patients came to the third follow-up. The OSDI and Oxford scores yielded statistically significant decreases, while Schirmer’s 1 test and FBUT values showed a statistically significant increase after treatment with vit D. A positive correlation was observed between vit D values and FBUT and Schirmer’s 1 values; there was a negative correlation between OSDI values (r=0.286 and p0.001, r=0.219 and p=0.032, r = -0.357 and p0.001, respectively). The dry eye findings improved significantly after vit D supplementation in all cases. Conclusion: During clinical follow-up, it is important to evaluate serum vit D levels in patients with dry eye syndrome (DES). Increasing their vit D levels will help clinicians to treat DES quickly and effectively.
- Published
- 2020
22. Polymethacryloyl-L-Phenylalanine [PMAPA]-Based Monolithic Column for Capillary Electrochromatography
- Author
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Sisem Ektirici, Suleyman Asir, Fatma Yılmaz, Adil Denizli, Ilgım Göktürk, and Ali Derazshamshir
- Subjects
Monolithic HPLC column ,Dopamine ,Phenylalanine ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Hydrophobic effect ,chemistry.chemical_compound ,Norepinephrine ,Capillary electrophoresis ,Catecholamines ,Capillary Electrochromatography ,Acetonitrile ,Capillary electrochromatography ,Chromatography ,010401 analytical chemistry ,Analytical technique ,General Medicine ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrophoresis ,chemistry ,Methacrylates ,0210 nano-technology ,Hydrophobic and Hydrophilic Interactions - Abstract
The ability to detect catecholamines (CAs) and their metabolites is vital to understand the mechanism behind the neuronal diseases. Neurochemistry aims to provide an improved pharmacological, molecular and physiological understanding of complex brain chemistries by analytical techniques. Capillary electrophoresis (CE) is one such analytical technique that enables the study of various chemical species ranging from amino acids and peptides to natural products and drugs. CE can easily adapt the changes in research focus and in recent years remains an applicable technique for investigating neuroscience and single cell neurobiology. The prepared phenylalanine-based hydrophobic monolithic column, Polymethacryloyl-L-phenylalanine [PMAPA], was used as a stationary phase in capillary electrochromatography to separate CAs that are similar in size and shape to each other including dopamine (DA) and norepinephrine (NE) via hydrophobic interactions. Separation carried out in a short period of 17 min was performed with the electrophoretic mobility of 5.54 × 10−6 m2 V−1 s−1 and 7.60 × 10−6 m2 V−1 s−1 for DA and NE, respectively, at pH 7.0, 65% acetonitrile ratio with 100 mbar applied pressure by the developed hydrophobic monolithic column without needing any extra process such as imprinting or spacer arms to immobilize ligands used in separation.
- Published
- 2018
23. Serum S100B, NSE and GRIA Levels as Neurological Markers in Lead Exposure
- Author
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Engin Tutkun, Esra Fırat Oğuz, Müjgan Ercan, Sevilay Sezer, Ömer Hınç Yılmaz, and Fatma Yılmaz
- Subjects
medicine.medical_specialty ,Central nervous system ,Enolase ,010501 environmental sciences ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,GRIA1 ,0105 earth and related environmental sciences ,Creatinine ,biology ,business.industry ,Glutamate receptor ,Neurotoxicity ,General Medicine ,medicine.disease ,medicine.anatomical_structure ,Endocrinology ,nervous system ,chemistry ,Lead exposure ,biology.protein ,Biomarker (medicine) ,business ,030217 neurology & neurosurgery - Abstract
Background/aim: The central nervous system is one of the major targets in lead exposure. Biomarkers for the diagnosis and follow-up of lead exposure have not been identified. In this study, serum S100B, neuron-specific enolase (NSE), and glutamate receptor 1 (GRIA1) levels were determined as possible biomarkers for lead neurotoxicity. Material and methods: Twenty-five subjects with chronic lead exposure and 25 controls were included in the study. NSE and S100B were measured by electrochemiluminescence immunoassay with a Cobas E601 analyzer. GRIA1 levels were measured with an ELISA kit using a quantitative sandwich enzyme immunoassay technique. Results: GRIA1 levels were significantly higher in the lead exposure group than in the control group. No significant differences for NSE, S100B, ALT, AST, or creatinine in sera were found between lead exposure and control groups. Conclusion: Subjects with chronic lead exposure are found to have increased glutamate receptor levels and do not seem to have glial or neuronal damage, which can be demonstrated with the elevation of NSE and S100B levels. GRIA1 levels might be used as a biomarker for the neurotoxicity of lead.
- Published
- 2018
24. Triazine herbicide imprinted monolithic column for capillary electrochromatography
- Author
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Suleyman Asir, Ali Derazshamshir, Adil Denizli, and Fatma Yılmaz
- Subjects
chemistry.chemical_classification ,Capillary electrochromatography ,Chromatography ,Monolithic HPLC column ,Aqueous solution ,Clinical Biochemistry ,Molecularly imprinted polymer ,Polymer ,Biochemistry ,Analytical Chemistry ,Electrophoresis ,chemistry ,Trietazine ,Molecule - Abstract
Trietazine was selectively separated from aqueous solution containing the competitor molecule cyanazine, which is similar in size and shape to the template molecule. Structural features of the molecularly imprinted column were figured out by SEM. The influence of the mobile-phase composition, applied electrical field, and pH of the mobile phase on the recognition of trietazine by the imprinted monolithic polymer has been evaluated, and the imprint effect in the trietazine-imprinted monolithic polymer was demonstrated by an imprinting factor. The optimized monolithic column resulted in separation of trietazine from a structurally related competitor molecule, cyanazine. In addition, fast separation was obtained within 6 min by applying higher electrical field, with the electrophoretic mobility of 2.97 × 10(-8) m(2) V(-1) s(-1) at pH 11.0.
- Published
- 2015
25. Megaporous poly(hydroxy ethylmethacrylate) based poly(glycidylmethacrylate-N-methacryloly-(l)-tryptophan) embedded composite cryogel
- Author
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Işık Perçin, Ali Derazshamshir, Deniz Türkmen, Nilay Bereli, Huma Shaikh, and Fatma Yılmaz
- Subjects
Composite number ,Fungal Proteins ,Contact angle ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Adsorption ,Polymethacrylic Acids ,Specific surface area ,Physical and Theoretical Chemistry ,Lipase ,Polyhydroxyethyl Methacrylate ,Candida ,Dispersion polymerization ,Chromatography ,biology ,Chemistry ,Osmolar Concentration ,Temperature ,Tryptophan ,Membranes, Artificial ,Surfaces and Interfaces ,General Medicine ,Hydrogen-Ion Concentration ,Monomer ,Membrane ,Chemical engineering ,Microscopy, Electron, Scanning ,biology.protein ,Electrophoresis, Polyacrylamide Gel ,Hydrophobic and Hydrophilic Interactions ,Porosity ,Cryogels ,Chromatography, Liquid ,Biotechnology - Abstract
One-step activation, purification, and stabilization of lipase enzyme were performed by using composite hydrophobic support at low ionic strength with increased surface area during embedding process. A novel hydrophobic poly(hydroxyethylmethacrylate) [PHEMA] based, poly(glycidyl methacrylate-N-methacryloly-(L)-tryptophan) [PGMATrp] bead embedded composite cryogel membrane having specific surface area of 195 m(2)/g was used as hydrophobic matrix for adsorption of commercial Candida Rugosa lipase in a continuous system. PGMATrp embedded PHEMA cryogel membrane with 60-100 mu m pore size was obtained by dispersion polymerization of GMA and MATrp to form PGMATrp beads followed by embedding of PGMATrp to HEMA via APS and TEMED redox pair. The introduction of hydrophobic MATrp monomer into bead structure aiming to increase interaction between lipase and composite membrane was estimated using nitrogen stoichiometry of elemental analysis and found to be 239 mu mol/g of polymer. Hydophobicity increment due to embedding process was confirmed by measuring contact angle, it was found 42 degrees and 48.4 degrees for the PHEMA and PHEMA/PGMATrp composite cryogel respectively. Some parameters i.e. pH, flow-rate, protein concentration, temperature, salt type and ionic intensity were evaluated on the adsorption capacity in a continuous system. Fast protein liquid chromatography (FPLC) studies were performed for specific adsorption of lipase onto the PHEMA/PGMATrp embedded composite cryogel membrane. (C) 2015 Elsevier B.V. All rights reserved.
- Published
- 2015
26. Removal of iron by chelation with molecularly imprinted supermacroporous cryogel
- Author
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Ilgım Göktürk, Duygu Çimen, and Fatma Yılmaz
- Subjects
inorganic chemicals ,Materials science ,Iron ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,Iron Chelating Agents ,Ion ,Molecular Imprinting ,chemistry.chemical_compound ,Molecular recognition ,Adsorption ,Glutamates ,Specific surface area ,Humans ,Chelation ,Aqueous solution ,Chromatography ,General Medicine ,Monomer ,chemistry ,Methacrylates ,Selectivity ,Porosity ,Cryogels ,Biotechnology ,Nuclear chemistry - Abstract
Iron chelation therapy can be used for the selective removal of Fe(3+) ions from spiked human plasma by ion imprinting. N-Methacryloyl-(L)-glutamic acid (MAGA) was chosen as the chelating monomer. In the first step, MAGA was complexed with the Fe(3+) ions to prepare the precomplex, and then the ion-imprinted poly(hydroxyethyl methacrylate-N-methacryloyl-(L)-glutamic acid) [PHEMAGA-Fe(3+)] cryogel column was prepared by cryo-polymerization under a semi-frozen temperature of - 12°C for 24 h. Subsequently, the template, of Fe(3+) ions was removed from the matrix by using 0.1 M EDTA solution. The values for the specific surface area of the imprinted PHEMAGA-Fe(3+) and non-imprinted PHEMAGA cryogel were 45.74 and 7.52 m(2)/g respectively, with a pore size in the range of 50-200 μm in diameter. The maximum Fe(3+) adsorption capacity was 19.8 μmol Fe(3+)/g cryogel from aqueous solutions and 12.28 μmol Fe(3+)/g cryogel from spiked human plasma. The relative selectivity coefficients of ion-imprinted cryogel for Fe(3+)/Ni(2+) and Fe(3+)/Cd(2+) were 1.6 and 4.2-fold greater than the non-imprinted matrix, respectively. It means that the PHEMAGA-Fe(3+) cryogel possesses high selectivity to Fe(3+) ions, and could be used many times without significantly decreasing the adsorption capacity.
- Published
- 2015
27. An alternative marker of low-density lipoprotein cholesterol in coronaryartery disease: non-high-density lipoprotein cholesterol
- Author
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Kübranur Ünal, Müjgan Ercan, Sema Uysal, Deniz Sahin, Nihal Boğdaycioğlu, Fatma Yılmaz, and Esra Oğuz
- Subjects
Adult ,medicine.medical_specialty ,Lipoproteins ,Low density lipoprotein cholesterol ,Coronary Artery Disease ,AutoAnalyzer ,Coronary artery disease ,chemistry.chemical_compound ,Internal medicine ,Humans ,Medicine ,In patient ,Aged ,Triglyceride ,business.industry ,Non high density lipoprotein cholesterol ,Significant difference ,nutritional and metabolic diseases ,Cholesterol, LDL ,General Medicine ,Middle Aged ,medicine.disease ,Cholesterol ,Endocrinology ,chemistry ,Case-Control Studies ,Non-high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,coronary artery disease ,lipids (amino acids, peptides, and proteins) ,business ,Biomarkers ,Dyslipidemia - Abstract
Dyslipidemia is one of the most important risk factors for coronary artery disease (CAD), and low-density lipoprotein cholesterol (LDL-C) is used to measure dyslipidemia. Non-high-density lipoprotein cholesterol (non-HDL-C) seems to be an alternative parameter to LDL-C as it is not influenced by triglyceride (TG) levels. The aim of this study is to compare non-HDL-C and LDL-C levels as risk markers in CAD patients. Materials and methods: One hundred and ten CAD patients and 42 individuals with normal coronary angiography results were included in this study. Patients were divided into 2 groups: TG < 200 mg/dL (n = 75) as group 1 and TG > 200 mg/dL (n = 35) as group 2. Total cholesterol (TC), TG, and HDL-C levels were analyzed with a Roche Modular P800 autoanalyzer. LDL-C and non-HDL-C levels were calculated. Results: There were statistically significant differences in TC, TG, HDL-C, and non-HDL-C levels when the groups were compared. Non-HDL-C levels of group 2 were statistically higher than those of group 1 and the control group. There was no significant difference in LDL-C levels between the groups. Conclusion: Non-HDL-C levels are better risk markers than LDL-C levels, especially in patients with TG > 200 mg/dL, and non-HDL-C levels should be taken into consideration when evaluating the risk of CAD.
- Published
- 2015
28. Molecularly imprinted hydrophobic polymers as a tool for separation in capillary electrochromatography
- Author
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Fatma Yılmaz, Adil Denizli, and Ali Derazshamshir
- Subjects
chemistry.chemical_classification ,endocrine system ,Capillary electrochromatography ,Aqueous solution ,Monolithic HPLC column ,Chromatography ,General Chemical Engineering ,General Engineering ,Polymer ,Analytical Chemistry ,chemistry.chemical_compound ,Monomer ,chemistry ,Chemical engineering ,Molecule ,Alkylbenzenes ,Selectivity - Abstract
The use of molecular imprinted polymers (MIPs), which provides a means for preparing stationary phases with predetermined selectivity for a target molecule in capillary electrochromatography (CEC), is attractive because it combines selectivity, higher separation efficiency and shorter analysis time. A bisphenol A (BPA)-imprinted monolithic capillary BPA/PMAPA column was synthesized for the selective separation of bisphenol A (BPA) from aqueous solutions containing the competitor molecule phenol (PH), which is similar in size and shape to the template molecule. BPA-imprinted monolithic column was prepared in the presence of the template molecule, BPA, which results in the formation of recognition cavities complementary to the template molecule, after the removal of template molecule. An amino acid based monomer, N-methacryloyl-L-phenyl alanine (MAPA), was used as the functional monomer. The new stationary phase contains both charged and hydrophobic groups originating from MAPA monomer, which behaves as both an electroosmotic flow (EOF) supplier and a hydrophobic matrix. The MAPA containing BPA imprinted column behaves as a mixed mode stationary phase, as ion exchanger and hydrophobic matrix depending on the pH of the medium. Scanning electron microscopy was used to identify the structural features of the molecular imprinted column. MIP column performance was evaluated by the electrochromatographic separation of alkylbenzenes. The novelty of this work originated from dual separation mechanism shown by MAPA, which has the ability to form both hydrophobic and electrostatic interactions by the charged and hydrophobic groups of phenylalanine amino acid. This new column with mixed-mode characteristics was then used successfully as the stationary phase in CEC for the selective separation of BPA in MIP systems.
- Published
- 2015
29. Dopamine-imprinted monolithic column for capillary electrochromatography
- Author
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Adil Denizli, Duygu Sari, Koray Şarkaya, Fatma Yılmaz, Suleyman Asir, and Ali Derazshamshir
- Subjects
Monolithic HPLC column ,Scanning electron microscope ,Dopamine ,Clinical Biochemistry ,02 engineering and technology ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,law.invention ,Molecular Imprinting ,Magazine ,law ,Capillary Electrochromatography ,Pressure ,Molecule ,chemistry.chemical_classification ,Capillary electrochromatography ,Chromatography ,Aqueous solution ,010401 analytical chemistry ,Reproducibility of Results ,Musa ,Polymer ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrophoresis ,chemistry ,0210 nano-technology - Abstract
A dopamine-imprinted monolithic column was prepared and used in capillary electrochromatography as stationary phase for the first time. Dopamine was selectively separated from aqueous solution containing the competitor molecule norepinephrine, which is similar in size and shape to the template molecule. Morphology of the dopamine-imprinted column was observed by scanning electron microscopy. The influence of the organic solvent content of mobile phase, applied pressure and pH of the mobile phase on the recognition of dopamine by the imprinted monolithic column has been evaluated, and the imprinting effect in the dopamine-imprinted monolithic polymer was verified. Developed dopamine-imprinted monolithic column resulted in excellent separation of dopamine from structurally related competitor molecule, norepinephrine. Separation was achieved in a short period of 10 min, with the electrophoretic mobility of 5.81x10(-5)m(2)V(-1)s(-1) at pH 5.0 and 500mbar pressure.
- Published
- 2017
30. The impact of levothyroxine sodium treatment on dynamic thiol/disulphide homeostasis in overt hypothyroidism
- Author
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Ihsan Ates, Canan Topcuoglu, Cemile Biçer, Murat Aydin, Salim Neselioglu, Ozcan Erel, Mustafa Altay, and Fatma Yılmaz
- Subjects
medicine.medical_specialty ,Endocrinology ,Chemistry ,Internal medicine ,medicine ,Thiol disulphide homeostasis ,Levothyroxine Sodium - Published
- 2017
31. Development of surface plasmon resonance sensors based on molecularly imprinted nanofilms for sensitive and selective detection of pesticides
- Author
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Semra Akgönüllü, Adil Denizli, Ali Derazshamshir, Nilay Bereli, Duygu Çimen, Fatma Yılmaz, and Yeşeren Saylan
- Subjects
Detection limit ,Alternative methods ,Chemistry ,Ethylene glycol dimethacrylate ,010401 analytical chemistry ,Metals and Alloys ,Nanotechnology ,02 engineering and technology ,Pesticide ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Phenylalanine methyl ester ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Functional monomer ,chemistry.chemical_compound ,Materials Chemistry ,Electrical and Electronic Engineering ,Surface plasmon resonance ,0210 nano-technology ,Molecular imprinting ,Instrumentation - Abstract
Pesticides have been utilized in agriculture for decades. However, their widespread use has increased multiple concerns due to their known and suspected toxicities on long-term human health risks in scientific and industrial communities. Thus, detecting pesticides will have a great impact on their management, as well as improve their toxicity effects over humans. Here, we fabricate molecularly imprinted nanofilms and integrate them with surface plasmon resonance (SPR) sensors for sensitive, selective, fast and real-time detection of multiple pesticides, including cyanazine (SNZ), simazine (SMZ) and atrazine (ATZ). The molecularly imprinted nanofilms onto the SPR gold surfaces are prepared via UV polymerization reactions, which consist of N-methacryloyl-L-phenylalanine methyl ester (MAPA) as a functional monomer, 1-vinylimidazole (VIM) as a co-monomer, and ethylene glycol dimethacrylate (EGDMA) as a cross-linker. The real-time measurements on SPR sensor provide a detection range from 0.10 to 6.64 nM, as well as denote a limit of detection (LOD) values of 0.095, 0.031 and 0.091 nM for SNZ, SMZ and ATZ, respectively. Furthermore, we perform selectivity test, where SNZ, SMZ and ATZ are examined as competitor agents. Overall, the pesticide imprinted SPR sensors have been found to be highly selective and sensitive. These SPR sensors also hold great potential to be used an alternative method for the existing pesticide monitoring approaches due to their reusability, fast response, and easy-to-use properties, as well as can be tailored to detect and real-time monitor of other pesticides. (C) 2016 Elsevier B.V. All rights reserved.
- Published
- 2017
32. Alterations of Thyroid Hormone Levels in Cadmium Exposure
- Author
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Meşide Gündüzöz, Evren Akgol, Ali Ünlü, Fatma Yılmaz, Engin Tutkun, Sedat Abusoglu, Ceylan Bal, Hınç Yılmaz, and Selçuk Üniversitesi
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Cadmium ,business.industry ,Thyroid Functions ,lcsh:R ,Thyroid ,lcsh:Medicine ,chemistry.chemical_element ,General Medicine ,Thyroxine ,03 medical and health sciences ,CADMIUM EXPOSURE ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Internal medicine ,medicine ,Cadmium Toxicity ,business ,Workers ,Hormone - Abstract
WOS: 000402382500007, Aim: Environmental chemicals and heavy metals may alter thyroid hormone levels via several mechanisms, including disruption of iodine (I) transport, thyroid peroxi-dase, thyroid hormone-binding proteins, hepatic catabolism, deiodinases, and receptor binding. Our aim was to investigate the change in thyroid hormone levels in cadmium exposure. Material and Method: Painters, welders, miners, and smelters with an occupational exposure of more than 10 years, aged between 18-70 years, were divided into six groups according to whole blood cadmium levels (Group 1: 0-0.5 mu g/L; Group 2: 0.5-1 mu g/L; Group 3: 1-1.5 mu g/L; Group 4: 1.5-2 mu g/L; Group 5: 2-2.5 mu g/L; Group 6: > 2.5 mu g/L). Results: There was a positive correlation between cadmium and serum free thyroxine and triiodothyronine levels. There was a negative correlation between cadmium and serum alanine aminotransferase and vitamin B12 levels. Discussion: Cadmium exposure was found to lead to an increase in thyroid hormone levels.
- Published
- 2017
33. Preparation and evaluation of open tubular C18-silica monolithic microcartridges for preconcentration of peptides by on-line solid phase extraction capillary electrophoresis
- Author
-
Estela Giménez, Elena Ortiz-Villanueva, Fatma Yılmaz, Victoria Sanz-Nebot, and Fernando Benavente
- Subjects
Chromatography ,Chemistry ,Capillary action ,Molecular Sequence Data ,Solid Phase Extraction ,Extraction (chemistry) ,Electrophoresis, Capillary ,Repeatability ,Substance P ,Standard solution ,Silicon Dioxide ,Mass spectrometry ,Biochemistry ,Mass Spectrometry ,Peptide Fragments ,Analytical Chemistry ,Capillary electrophoresis ,Limit of Detection ,Environmental Chemistry ,Amino Acid Sequence ,Solid phase extraction ,Selectivity ,Oligopeptides ,Spectroscopy - Abstract
In this study, C18-silica monoliths were synthesized as a porous layer in open tubular capillary columns, to be cut later into microcartridges for the analysis of neuropeptides by on-line solid-phase extraction capillary electrophoresis with UV and MS detection (SPE-CE-UV and SPE-CE-MS). First, several types of C18-silica monolithic (MtC18) microcartridges were used to analyse standard solutions of five neuropeptides (i.e. dynorphin A (1–7), substance P (7–11), endomorphin 1, methionine enkephalin and [Ala]-methionine enkephalin). The MtC18 sorbents were especially selective against endomorphin 1 and substance P (7–11)). The best results in terms of sensitivity and inter-microcartridge reproducibility were achieved with the microcartridges obtained from a 10-cm open tubular capillary column with a thin monolithic coating with large through-pores (1–5 μm). Run-to-run repeatability, microcartridge durability, linearity ranges and LODs were studied by MtC18-SPE-CE-MS. As expected due to their greater selectivity, the best LOD enhancement was obtained for End1 and SP (7–11) (50 times with regard to CE-MS). Finally, the suitability of the methodology for analysing biological fluids was tested with plasma samples spiked with End1 and SP (7–11). Results obtained were promising because both neuropeptides could be detected at 0.05 μg mL −1 , which was almost the same concentration level as for the standard solutions (0.01 μg mL −1 ).
- Published
- 2014
34. Synthesis of hydrophobic nanoparticles for real-time lysozyme detection using surface plasmon resonance sensor
- Author
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Fatma Yılmaz, Adil Denizli, Yeşeren Saylan, Erkut Yılmaz, Ali Derazshamshir, Sabire Yazıcı Fen Edebiyat Fakültesi, and yilmaz, erkut -- 0000-0002-1217-5225
- Subjects
Materials science ,education ,Analytical chemistry ,Nanoparticle ,02 engineering and technology ,Biosensing Techniques ,01 natural sciences ,Nanomaterials ,Contact angle ,symbols.namesake ,chemistry.chemical_compound ,Hemoglobins ,Adsorption ,Hydrophobic Nanoparticle ,Structural Biology ,Surface plasmon resonance ,Molecular Biology ,lysozyme ,Sensor ,Aqueous solution ,Myoglobin ,010401 analytical chemistry ,Langmuir adsorption model ,Surface Plasmon Resonance ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Kinetics ,chemistry ,symbols ,Nanoparticles ,Muramidase ,Lysozyme ,0210 nano-technology ,Hydrophobic and Hydrophilic Interactions - Abstract
WOS: 000407478900004, PubMed: 28322473, Diagnostic biomarkers such as proteins and enzymes are generally hard to detect because of the low abundance in biological fluids. To solve this problem, the advantages of surface plasmon resonance (SPR) and nanomaterial technologies have been combined. The SPR sensors are easy to prepare, no requirement of labelling and can be detected in real time. In addition, they have high specificity and sensitivity with low cost. The nanomaterials have also crucial functions such as efficiency improvement, selectivity, and sensitivity of the detection systems. In this report, an SPR-based sensor is developed to detect lysozyme with hydrophobic poly (N-methacryloyl-(L)-phenylalanine) (PMAPA) nanoparticles. The SPR sensor was first characterized by attenuated total reflection-Fourier transform infrared, atomic force microscope, and water contact angle measurements and performed with aqueous lysozyme solutions. Various concentrations of lysozyme solution were used to calculate kinetic and affinity coefficients. The equilibrium and adsorption isotherm models of interactions between lysozyme solutions and SPR sensor were determined and the maximum reflection, association, and dissociation constants were calculated by Langmuir model as 4.87, 0.019nM(-1), and 54nM, respectively. The selectivity studies of SPR sensor were investigated with competitive agents, hemoglobin, and myoglobin. Also, the SPR sensor was used four times in adsorption/desorption/recovery cycles and results showed that, the combination of optical SPR sensor with hydrophobic ionizable PMAPA nanoparticles in one mode enabled the detection of lysozyme molecule with high accuracy, good sensivity, real-time, label-free, and a low-detection limit of 0.66nM from lysozyme solutions. Lysozyme detection in a real sample was performed by using chicken egg white to evaluate interfering molecules present in the medium.
- Published
- 2016
35. Non-HDL cholesterol, total cholesterol/HDL cholesterol and LDL-cholesterol levels in patients with acute ischemic stroke
- Author
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Fatma Yılmaz, Nihal Boğdaycıoğlu, Semra Mungan Öztürk, Sema Uysal, and Müjgan Ercan
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medicine.medical_specialty ,Neurology ,Triglyceride ,business.industry ,Cholesterol ,Blood lipids ,General Medicine ,Blood flow ,Neurological disorder ,medicine.disease ,AutoAnalyzer ,Gastroenterology ,chemistry.chemical_compound ,chemistry ,Internal medicine ,medicine ,lipids (amino acids, peptides, and proteins) ,Thrombus ,business - Abstract
Objective: Ischemic stroke is defined as acute neurological disorder according to cerebral vascular damage and reduced blood flow to a particular region of the brain. Ischemic stroke is a major cause of morbidity and mortality and placed 3rd among the causes of death. About 80% of all strokes depend on ischemic cause. Atherosclerotic plaque rupture or addition of a thrombus on plaque is the most common cause of ischemic stroke. Atherosclerosis is associated with the level of blood lipids from the first stages of the formation process. In our study, we aimed to investigate non-HDL, total cholesterol / HDL-cholesterol and LDL-cholesterol levels in patients with acute ischemic stroke. Methods: This study was performed in 52 patients who were admitted at Ankara Numune Hospital for Education and Investigation, Division of Neurology between December 2009- May 2010 with a diagnosis of acute ischemic stroke in the first 24 hours and 48 healthy individuals matched for age and gender, who was admitted at the hospital during the same period. Total cholesterol, triglycerides and HDL cholesterol levels were measured by DXC 800 autoanalyzer (Beckmann-Coulter, USA). LDL cholesterol, total cholesterol / HDL-cholesterol and non-HDL cholesterol levels were calculated according to the formulas. Results: Patient group had significantly lower HDL-C levels than the control group (p=0.009), whereas the triglyceride and total cholesterol / HDL-C levels were significantly higher (p=0.013, p=0.018). Total cholesterol, LDL cholesterol and non-HDL cholesterol levels did not differ significantly between groups. There were significant correlations between non-HDL-cholesterol levels and triglycerides, total cholesterol, LDL cholesterol (respectively r=0.468, r=0.964, r=0.851) (respectively p
- Published
- 2013
36. Enantioseparation of aromatic amino acids using CEC monolith with novel chiral selector,N-methacryloyl-<scp>l</scp>-histidine methyl ester
- Author
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Duygu Çimen, Lokman Uzun, Adil Denizli, Cemil Aydoğan, and Fatma Yılmaz
- Subjects
Acetonitriles ,Monolithic HPLC column ,Clinical Biochemistry ,Stereoisomerism ,Biochemistry ,Analytical Chemistry ,Amino Acids, Aromatic ,chemistry.chemical_compound ,Capillary Electrochromatography ,Spectroscopy, Fourier Transform Infrared ,Pressure ,Aromatic amino acids ,Histidine ,Monolith ,In situ polymerization ,chemistry.chemical_classification ,geography ,geography.geographical_feature_category ,Chromatography ,Chemistry ,technology, industry, and agriculture ,Amino acid ,Chiral column chromatography ,Methacrylates ,Enantiomer ,Nuclear chemistry - Abstract
A new type of polymethacrylate-based monolithic column with chiral stationary phase was prepared for the enantioseparation of aromatic amino acids, namely D,L-phenylalanine, D,L-tyrosine, and D,L-tryptophan by CEC. The monolithic column was prepared by in situ polymerization of butyl methacrylate (BMA), N-methacryloyl-L-histidine methyl ester (MAH), and ethylene dimethacrylate (EDMA) in the presence of porogens. The porogen mixture included DMF and phosphate buffer. MAH was used as a chiral selector. FTIR spectrum of the polymethacrylate-based monolith showed that MAH was incorporated into the polymeric structure via in situ polymerization. Some experimental parameters including pH, concentration of the mobile phase, and MAH concentration with regard to the chiral CEC separation were investigated. Single enantiomers and enantiomer mixtures of the amino acids were separately injected into the monolithic column. It was observed that L-enantiomers of aromatic amino acids migrated before D-enantiomers. The reversal enantiomer migration order for tryptophan was observed upon changing of pH. Using the chiral monolithic column (100 μm id and 375 μm od), the best chiral separation was performed in 35:65% ACN/phosphate buffer (pH 8.0, 10 mM) with an applied voltage of 12 kV in CEC. SEM images showed that the chiral monolithic column has a continuous polymeric skeleton and large through-pore structure.
- Published
- 2013
37. Cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins by nano liquid chromatography†
- Author
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Adil Denizli, Cemil Aydoğan, and Fatma Yılmaz
- Subjects
chemistry.chemical_classification ,geography ,geography.geographical_feature_category ,Monolithic HPLC column ,Chromatography ,Ion exchange ,technology, industry, and agriculture ,Azobisisobutyronitrile ,Filtration and Separation ,Analytical Chemistry ,Hydrophobic effect ,chemistry.chemical_compound ,Monomer ,chemistry ,Monolith ,Boronic acid ,Alkyl - Abstract
In this study, vinyl phenyl boronic acid modified lauryl methacrylate-based monolithic column was successfully prepared for cation exchange/hydrophobic interaction monolithic chromatography of small molecules and proteins in nano LC. The polymeric mixture consisted of lauryl methacrylate, vinyl phenyl boronic acid as cation exchanger, ethylene dimethacrylate as cross-linker, polyethylene glycol and methanol as binary porogenic solvent, and azobisisobutyronitrile as initiator. The resulting monolith showed good permeability and mechanical stability. Different ratios of monomer and porogens were used for optimizing the properties of the column. The monolithic column performance with respect to hydrophobic and cation exchange interactions was assessed by the separation a series of alkyl benzenes and anilines, respectively. cis-Diol-containing compounds such as phenols were also utilized to evaluate the retention behaviors of the vinyl phenyl boronic acid modified monolithic column. The monolithic column showed cation exchange interactions in the separation of aniline compounds. Theoretical plate number up to 52 000 plates/m was successfully achieved. The prepared monolith was further applied to the proteins with different acetonitrile content.
- Published
- 2013
38. An underestimated preanalytical error source: centrifuge temperature
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Fatma Yılmaz, Serkan Kiral, Nihal Boğdaycıoğlu, and Sema Uysal
- Subjects
Working hours ,Centrifuge ,Temperature control ,Chromatography ,Chemistry ,Biochemistry (medical) ,Clinical Biochemistry ,Thyroid panel ,Analytical chemistry ,Molecular Biology ,Biochemistry ,Total protein - Abstract
Objectives: The study demonstrates a gel problem sourced from the internal heat of centrifuges without temperature control. It was planned to investigate the internal temperatures of temperature controlled centrifuges and the centrifuges without temperature control and the effect of centrifuge temperature on enzymes and thyroid panel. Design and Methods: Internal heat of the centrifuges were monitored in the most busy working hours. Blood samples were obtained from 42 patients to two separate biochemistry tubes. One sample of the same patient was centrifuged with a temperature-controlled centrifuge (Group 1) and the other was centrifuged with a centrifuge without temperature control (Group 2). AST, ALT, GGT, LDH, total protein, albumin, TSH, fT3 and fT4 levels were determined in the same run of the analyzer. Results: ALT and TSH values in Group 2 were significantly lower, fT4 values were significantly higher than Group 1. Conclusions: Laboratories should be aware of a possible internal heat production in centrifuges without temperature control and gel tubes should be centrifuged in temperature controlled centrifuges especially in centrifuge units with high working load.
- Published
- 2013
39. Poly(vinyl alcohol)/polyethyleneimine (PVA/PEI) blended monolithic cryogel columns for the depletion of haemoglobin from human blood
- Author
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Fatma Yılmaz, Adil Denizli, Ilgım Göktürk, and Ali Derazshamshir
- Subjects
Vinyl alcohol ,Aqueous solution ,Chromatography ,Human blood ,Process Chemistry and Technology ,General Chemical Engineering ,010401 analytical chemistry ,Filtration and Separation ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,Adsorption ,chemistry ,Human plasma ,Ionic strength ,Chelation ,0210 nano-technology ,Nuclear chemistry - Abstract
We have synthesized PVA/PEI monolithic cryogel columns chelated with Cu2+ ions as a model adsorbent, which is capable of binding haemoglobin (Hb) from human blood. The goal of this study is to perform the depletion of Hb via a single and easy process to be useful in proteomic studies. PVA/PEI-Cu2+ cryogel columns were subjected to adsorption studies of Hb from both aqueous solution and human plasma to evaluate the extent of interaction between cryogel columns and Hb. The effects of experimental parameters, such as pH, Hb equilibrium concentration, adsorption time, temperature, and ionic strength, on Hb adsorption capacity were investigated.
- Published
- 2016
40. Preparation of cryogel columns for depletion of hemoglobin from human blood
- Author
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Gözde Baydemir, Adil Denizli, Fatma Yılmaz, Nilay Bereli, Ali Derazshamshir, Sabire Yazıcı Fen Edebiyat Fakültesi, BAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümü, and Yılmaz, Fatma
- Subjects
Materials science ,Base (chemistry) ,Iminodiacetic acid ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,02 engineering and technology ,Methacrylate ,Hemolysis ,01 natural sciences ,Matrix (chemical analysis) ,Metal Chelate Affinity Sorbents ,Metal Chelate Sffinity Sorbents ,Hemoglobins ,chemistry.chemical_compound ,Hemoglobin Depletion ,Humans ,Chelation ,Epichlorohydrin ,Polyhydroxyethyl Methacrylate ,chemistry.chemical_classification ,Chromatography ,Imino Acids ,010401 analytical chemistry ,General Medicine ,021001 nanoscience & nanotechnology ,PHEMA Cryogel ,0104 chemical sciences ,chemistry ,Covalent bond ,IDA ,Hemoglobin ,0210 nano-technology ,Cryogels ,Biotechnology - Abstract
WOS: 000376136500005, PubMed: 26772759, In this study, we aimed to prepare the metal chelate affinity cryogels for the hemoglobin (Hb) depletion. Poly(2-hydroxyethyl methacrylate) (PHEMA) cryogels were selected as base matrix because of their blood compatibility, osmotic, chemical, and mechanical stability. Cryogels are also useful when working with the viscous samples such as blood, because of their interconnected macroporous structure. Iminodiacetic acid (IDA), the chelating agent, was covalently coupled with PHEMA cryogels after activation with the epichlorohydrin and then the Ni(II) ions were chelated to the IDA-bound cryogels. The depletion of the Hb from hemolysate was shown by SDS-PAGE.
- Published
- 2016
41. Serum and Liver Tissue Bio-Element Levels, and Antioxidant Enzyme Activities in Carbon Tetrachloride-Induced Hepatotoxicity: Protective Effects of Royal Jelly
- Author
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Mustafa Cemek, Fatma Yılmaz, Ahmet Ayaz, Fatih Aymelek, Mehmet Emin Buyukokuroglu, and Ahmet Büyükben
- Subjects
Male ,Antioxidant ,food.ingredient ,medicine.medical_treatment ,Medicine (miscellaneous) ,Antioxidant potential ,Pharmacology ,Protective Agents ,Antioxidants ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,food ,Liver tissue ,Royal jelly ,medicine ,Animals ,Humans ,Liver damage ,Carbon Tetrachloride ,Vital organ ,chemistry.chemical_classification ,Nutrition and Dietetics ,Chemistry ,Liver Diseases ,Fatty Acids ,Enzymes ,Rats ,Enzyme ,Liver ,Biochemistry ,Carbon tetrachloride ,Blood Chemical Analysis - Abstract
The liver is a vital organ, and its function is generally impaired by chemicals. Some natural compounds have a protective role against liver diseases such as royal jelly (RJ). To our knowledge, there are no data available on the effect of RJ therapy on the levels of bio-element metabolisms and antioxidant enzyme activities in the carbon tetrachloride (CCl(4))-induced liver damage. Therefore, in the present study, we have investigated the role of RJ therapy in the trace and major elements and antioxidant enzymes in CCl(4)-induced hepatotoxicity in rats. Antioxidant enzyme activities decreased in the CCl(4)-treated group more than they did in the sham and RJ-administered groups. Many bio-element levels were also reduced in only the CCl(4)-treated group. This showed that the depletion of trace elements was related to erythrocyte antioxidant enzyme activities. RJ administration clearly increased the trace and major element levels and antioxidant enzyme activities in RJ groups. RJ may be used as functional foods because of their naturally high antioxidant potential and rich element content.
- Published
- 2012
42. Antibody purification from human plasma by metal-chelated affinity membranes
- Author
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Adil Denizli, Fatma Yılmaz, Handan Yavuz, Canan Armutcu, Nilay Bereli, BAİBÜ, Gerede Meslek Yüksekokulu, Kimya Ve Kimyasal İşleme Teknolojileri Bölümü, and Yılmaz, Fatma
- Subjects
Immunoglobulin-G ,inorganic chemicals ,Polymers and Plastics ,Surface Properties ,Metal ions in aqueous solution ,Chromatography, Affinity ,Immunoglobulin G ,Polymerization ,PHEMA ,Metal ,Affinity Membranes ,Adsorption ,Polyamines ,Materials Chemistry ,Humans ,Histidine ,Chelation ,Sulfhydryl Compounds ,Polyhydroxyethyl Methacrylate ,Chelating Agents ,Chromatography ,biology ,Elution ,Chemistry ,Membranes, Artificial ,General Chemistry ,Surfaces, Coatings and Films ,Membrane ,Metals ,visual_art ,visual_art.visual_art_medium ,biology.protein ,IMAC - Abstract
WOS:000298086500032 The aim of this study is to investigate in detail the feasibility of poly(2-hydroxyethyl methacrylate-N-methacryloyl-(L)-histidine methyl ester), PHEMAH membranes for purification of immunoglobulin G (IgG) from human plasma. PHEMAH membranes were prepared by photo-polymerization technique. Then, Zn2+, Ni2+, Co2+, and Cu2+ ions were chelated directly on the PHEMAH membranes. Elemental analysis assay was performed to determine the nitrogen content and polymerized MAH was calculated as 168.5 mu mol/g. The nonspecific IgG adsorption onto the plain PHEMA membranes was negligible (about 0.25 mg/mL). A remarkable increase in the IgG adsorption capacities were achieved from human plasma with PHEMAH membranes (up to 68.4 mg/mL). Further increase was observed with the metal-chelated PHEMAH membranes (up to 118 mg/mL). The metal-chelate affinity membranes allowed the one-step separation of IgG from human plasma. The binding range of metal ions for surface histidines from human plasma followed the order: Cu2+ > Ni2+ > Zn2+ > Co2+. Adsorbed IgG was eluted using 250 mM EDTA with a purity of 94.1%. IgG molecules could be repeatedly adsorbed and eluted with the metal-chelated PHEMAH membranes without noticeable loss in their IgG adsorption capacity. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 123: 3476-3484, 2012
- Published
- 2011
43. Bio-Element Status in Children With Acute Rheumatic Fever: Before Treatment and After Clinical Improvement
- Author
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Ahmet Büyükben, Fatih Aymelek, Mehmet Emin Buyukokuroglu, Murat Doğan, Derya Yıldırım, Fatih Sanli, Fatma Yılmaz, Mustafa Cemek, and Abdurrahman Üner
- Subjects
Male ,medicine.medical_specialty ,Adolescent ,chemistry.chemical_element ,Calcium ,Gastroenterology ,Pathogenesis ,Electrolytes ,Human health ,Internal medicine ,medicine ,Humans ,Child ,Cadmium ,business.industry ,Case-control study ,Acute rheumatic fever ,Elements ,Trace Elements ,Multisystem disease ,chemistry ,Case-Control Studies ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Female ,Rheumatic Fever ,Cardiology and Cardiovascular Medicine ,business ,Selenium - Abstract
Acute rheumatic fever (ARF) is an autoimmune multisystem disease. Bio-elements are required in different quantities by an organism to maintain its physiologic function. Monitoring the status of bio-elements is critical in human health. This study aimed to determine possible changes in levels of bio-elements in children with ARF before and after treatment. Levels of trace and major elements in children with ARF were investigated. The study included 33 children with ARF (17 boys and 16 girls) and 20 healthy control children (11 boys and 9 girls). The ages ranged from 5 to 16 years (mean 11.4 +/- A 3.82 years) in the study group and from 6 to 15 years (mean, 10.7 +/- A 3.22 years) in the control group. Trace and major element concentrations (total of 14 elements) in the serum were measured by inductively coupled plasma-optical emission spectroscopy. Before treatment, the levels of the major elements potassium (K) and magnesium (Mg) in children with ARF were higher than in the control group, whereas the calcium (Ca) level was lower. Before treatment, the levels of trace elements iron (Fe), selenium (Se), zinc (Zn), aluminum (Al), and barium (Ba) were lower, whereas the copper (Cu), beryllium (Be), cadmium (Cd), chromium (Cr), gallium (Ga), and strontium (Sr) levels were higher in the serum of the patients with ARF than in the control patients. The major findings show that the homeostasis of some trace and major elements were altered in the children with ARF and that these alterations may be a contributing factor in the pathogenesis of this disease.
- Published
- 2010
44. Supermacroporous hydrophobic affinity cryogels for protein chromatography
- Author
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Adil Denizli, Fatma Yılmaz, Nilay Bereli, and Handan Yavuz
- Subjects
Environmental Engineering ,Chromatography ,Aqueous solution ,Biomedical Engineering ,Bioengineering ,Methacryloyl chloride ,Hydrophobic effect ,chemistry.chemical_compound ,Adsorption ,chemistry ,Protein purification ,Lysozyme ,Ethylene glycol ,Biotechnology ,Egg white - Abstract
N -Methacryloyl- l -tryptophan (MATrp) containing poly(2-hydroxyethyl methacrylate) based supermacroporous cryogel [PHEMATrp] was prepared for lysozyme purification form chicken egg white. MATrp was synthesized by reacting methacryloyl chloride with l -tryptophan methyl ester and provided hydrophobic functionality to the cryogel. PHEMATrp cryogel with 60–100 μm pore size was obtained by free radical polymerization of HEMA and MATrp having a specific surface area of 50 m 2 /g. PHEMATrp cryogel was characterized by swelling studies, FTIR and SEM. The equilibrium swelling ratios of the cryogels were 7.18 g H 2 O/g for PHEMA and 6.99 g H 2 O/g for PHEMATrp. Lysozyme adsorption experiments were investigated under different conditions in continuous system (i.e., medium pH, flow-rate, protein concentration, temperature, salt type). Lysozyme adsorption capacity of PHEMA and PHEMATrp cryogels from aqueous solutions was estimated as 2.9 and 46.8 mg/g (0.49 and 7.85 mg/mL), respectively. Lysozyme molecules were desorbed with 0.5 M ethylene glycol solution with 91% recovery. It was observed that PHEMATrp cryogel can be used without significant decrease in lysozyme adsorption capacity after five adsorption–desorption cycles. PHEMATrp cryogel was used for the purification of lysozyme from chicken egg white. Purity of lysozyme was estimated by SDS-PAGE. Possible denaturation of purified lysozyme was checked with fluorimetric measurements. Specific activity of the purified lysozyme was found as 43,140 U/mg using Micrococcus lysodeikticus as substrate.
- Published
- 2009
45. Pseudospecific magnetic affinity beads for immunoglobulin-G depletion from human serum
- Author
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Adil Denizli, Fatma Yılmaz, Lokman Uzun, Serpil Özkara, and Sinan Akgöl
- Subjects
Chromatography ,Polymers and Plastics ,biology ,Chemistry ,Elution ,General Chemistry ,Immunoglobulin G ,Surfaces, Coatings and Films ,MOPS ,chemistry.chemical_compound ,Adsorption ,Affinity chromatography ,Ionic strength ,Materials Chemistry ,biology.protein ,Suspension polymerization ,Ethylene glycol - Abstract
Magnetic poly(ethylene glycol dimethacrylate-N-methacryloyl-(L)-histidine methyl ester) [m-poly(EGDMA-MAH)] beads were prepared by suspension polymerization for the affinity depletion of immunoglobulin-G (IgG) from human serum in a batch system. Elemental analysis of the magnetic beads for nitrogen was estimated as 70 μmol MAH/g polymer. IgG adsorption onto the m-poly(EGDMA) was negligible. Higher adsorption value (up to 46.8 mg/g) was obtained in which the m-poly(EGDMA-MAH) beads were used. IgG adsorption capacity of the magnetic beads increased with an increase in the concentration of IgG. The maximum IgG adsorption was observed at pH 6.5 for MOPS buffer. IgG molecules could be repeatedly adsorbed and eluted with these adsorbents, without noticeable loss in their IgG adsorption capacity. Adsorption capacity decreased for both increasing salt concentration and temperature. In this study, we show that m-poly(EGDMA-MAH) beads (wherein IgG molecules bind directly with the matrix) can be used directly for affinity depletion without further modification. Higher adsorption value was obtained from human serum (up to 85.7 mg/g). The elution results demonstrated that the adsorption of IgG to the adsorbent was reversible. The depletion efficiencies for IgG were above 85% for all studied concentrations. Eluted portion was analyzed for testing the IgG removal efficiency by two dimensional gel electrophoresis. Eluted proteins include mainly IgG, and a small number of nonalbumin proteins such as apolipoprotein A1, serotransferrin, haptoglobulin, and α1-antitrypsin. IgA was not identified in eluted fraction. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007
- Published
- 2007
46. Comparison of Improvacuter™ tubes with BD Vacutainer™ tubes for various hormones in the aspects of stability and influence of gel separators
- Author
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Canan Topcuoglu, Cevdet Züngün, Elif Guney Boru, and Fatma Yılmaz
- Subjects
Immunoassay ,Blood Specimen Collection ,Analyte ,Chromatography ,Chemistry ,Biochemistry (medical) ,Clinical Biochemistry ,Becton dickinson ,General Medicine ,Hormone assay ,Laboratory results ,Healthy Volunteers ,Hormones ,Blood Preservation ,Humans ,Blood Collection Tube ,Vacutainer ,Gels ,Blood Chemical Analysis - Abstract
BACKGROUND Validation of blood collection tubes are important to determine the role of different collection tubes which influence the assurance of laboratory results. We compared two different tubes (Improvacuter™ and Becton Dickinson [BD] Vacutainer™) and investigated the effect of gel and storage time in comparison with each other. METHODS We compared the results of nine immunoassays performed on UniCel® DxI 800 using blood samples collected in BD Vacutainer SST II Advance tubes, Improvacuter Gel and Clot Activator tubes, BD Vacutainer Clot Activator tubes and Improvacuter tubes. Analytes were measured in all tubes on 3 consecutive days to study the effect of long-term storage. Evaluation of clinical significance was performed based on total allowable error. RESULTS Estradiol and testosterone concentrations obtained from Improvacuter Gel and Clot Activator tubes and BD Vacutainer SST II Advance tubes remained below the lower limits of analytical range for the same analytes while they were within the limits in BD Vacutainer Clot Activator tubes and Improvacuter tubes. Statistical significance of stability was not clinically significant for the hormone parameters we tested in all four tubes. CONCLUSIONS Gel containing tubes (both BD and Improve) gave comparable results with the tubes which do not contain gel except for estradiol and testosterone. The use of gel containing tubes for estradiol and testosterone are not recommended on UniCel® DxI 800 according to our results. The change in the analyte concentrations over 48 h remained within the TEA limits for the studied analytes. Improve tubes gave similar results to BD tubes.
- Published
- 2015
47. Akut civa maruziyeti olan çocuklarda renal fonksiyonların farklı glomerüler fitrasyon hızıformülleriyle değerlendirilmesi
- Author
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Ceylan Bal, Hüseyin Çelik, Nihal Uğuz, Oya Torun Güngör, Fatma Yılmaz, Engin Tutkun, Sedat Abusoglu, Ömer Hınç Yılmaz, Metin Yildirimkaya, Selçuk Üniversitesi, and Kırşehir Ahi Evran Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri, Tıbbi Biyokimya ABD
- Subjects
glomerular filtration rate ,medicine.medical_specialty ,business.industry ,Biochemistry (medical) ,Clinical Biochemistry ,creatinine ,Urology ,Renal function ,chemistry.chemical_element ,Mercury ,urologic and male genital diseases ,Biochemistry ,female genital diseases and pregnancy complications ,Mercury (element) ,chemistry ,cystatin C ,Acute exposure ,medicine ,Biyokimya ve Moleküler Biyoloji ,business ,Molecular Biology ,reproductive and urinary physiology ,acute renal damage - Abstract
Amaç: Çocuklarda akut civa maruziyetine bağlı hızlı glomerüler fitrasyon hızı azalmalarında sistatin C düzeyinin böbrek fonksiyonlarını değerlendirme açısından kreatinine üstün olup olmadığını belirlemeyi amaçladık. Ayrıca kreatinin ve/veya sistatin C kullanılan 8 ayrı glomerular filtrasyon hızı hesaplama formülünü birbiriyle karşılaştırdık.Metod: Civa maruziyeti yaşayan 39 çocuğun serum üre, kreatinin ve sistatin C düzeyleri ölçüldü. Glomerular filtrasyon hızı 8 farklı formülle hesaplandı. Hasta grubu civa seviyelerine göre üç subgruba bölündü.Bulgular: Hastaların sistatin C ve civa düzeyleri kontrol grubundan anlamlı olarak farklı bulundu (p0.001). Kreatinin ve üre açısından iki grup arasında anlamlı bir fark bulunmadı (p0.913, p0.236). Serum kreatinini ve boy kullanılarak veya bunlara ilaveten üre değerleri kullanılarak yapılan GFR hesaplamalarında hasta ve kontrol grupları arasında anlamlı bir fark yokken (sırasıyla, p0.069, p0.559, p0.424, p0.945 ), yalnızca sistatin C veya buna ek olarak kreatinin, üre ve boyun kullanıldığı GFR hesaplamalarında hasta ve kontrol grupları arasında anlamlı bir fark vardı (sırasıyla, p0.001, p0.001, p0.042, p0.001). Subgrup analizinde sistatin C sonuçları ile sistatin C kullanılarak hesaplanan dört GFR hesaplamasından üç tanesine ait sonuçlar kontrol grubunda subgruplara göre farklı bulunurken subgruplar arasında farklı bulunmadı.Sonuç: Akut maruziyetlere bağlı hızlı glomerüler fitrasyon hızı azalmalarında sistatin C düzeyi böbrek fonksiyonlarını değerlendirme açısından kreatinine üstündür. Sistatin C kullanılarak oluşturulan formüller glomerüler filtrasyon hızını belirlemede kreatinin ve boy uzunluğu kullanılarak oluşturulan formüllere kıyasla daha iyi sonuç vermektedir., Objective: Our aim was to determine whether cystatin C level has a superiority to creatinine to assess kidney functions in rapid decreases of glomerular filtration rate due to acute mercury exposure in children. Eight different glomerular filtration rate calculation formulas which have been used creatinine and/or cystatin C were also compared.Methods: Serum urea, creatinine and cystatin C values of 39 mercury exposed children were measured. Glomerular filtration rates were calculated by eight different formulas. Patient group was divided into three subgroups according to mercury levels.Results: Cystatin C and mercury levels of the patients were found significantly different from control group (p
- Published
- 2015
48. Antibody Purification from Human Plasma by Metal-Chelated Affinity Membranes
- Author
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Nilay Bereli, Fatma Yılmaz, Adil Denizli, Canan Armutcu, and Handan Yavuz
- Subjects
Metal ,Tandem affinity purification ,chemistry.chemical_compound ,Membrane ,Affinity chromatography ,Chemistry ,Ligand ,Metal ions in aqueous solution ,visual_art ,visual_art.visual_art_medium ,Chelation ,(Hydroxyethyl)methacrylate ,Nuclear chemistry - Abstract
Immobilized metal ion affinity chromatography (IMAC) has been used for purification of proteins. IMAC introduces a new approach for selectively interacting biomolecules on the basis of their affinities for metal ions. The separation is based on different binding abilities of the proteins to the chelated metal ions on support. Here, N-methacryloyl-(L)-histidine methyl ester (MAH) is used as the metal-chelating ligand. Poly(hydroxyethyl methacrylate) Poly(HEMA) based membranes were prepared by photo-polymerization technique. Then, Zn(2+), Ni(2+), Co(2+), and Cu(2+) ions were chelated directly on the poly(HEMA-MAH) membranes for purification of immunoglobulin G (IgG) from human plasma.
- Published
- 2015
49. Effect of povidone iodine on thyroid functions and urine iodine levels in caesarean operations
- Author
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Gulsen Yilmaz, Jale Karakaya, Fatma Yılmaz, Huseyin Tugrul Celik, Ugur Hamurcu, and Rahime Bedir Findik
- Subjects
Adult ,Thyroid Hormones ,medicine.medical_specialty ,Urine iodine ,Group ii ,Thyroid Gland ,chemistry.chemical_element ,Thyroid Function Tests ,Administration, Cutaneous ,Iodine ,Excretion ,Pregnancy ,Internal medicine ,medicine ,Humans ,Povidone-Iodine ,Cesarean Section ,business.industry ,Thyroid ,Infant, Newborn ,Obstetrics and Gynecology ,Patient Outcome Assessment ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Thyroid hormones ,Pediatrics, Perinatology and Child Health ,Anti-Infective Agents, Local ,Female ,business ,Biomarkers - Abstract
In the present study, the effects of povidone iodine (PI) used during Caesarean operations on maternal thyroid hormones and urine iodine levels in the infant and the mother were investigated.Twenty-seven patients were allocated to the PI group I (Gr I), and 28 to the non-PI group (Group II). Maternal preoperative and 24th-h postoperative free T3 (fT3), freeT4 (fT4), TSH, and urine iodine levels were determined, as well as infant urine iodine values.In both groups, fT3 levels before and after the operation were found to be reduced (p 0.01, p = 0.01, respectively). However, the decrease in fT3 was approximately 1.5-fold higher in Gr I than in Gr II. TSH levels increased significantly in Gr I (p = 0.005). The urine iodine levels of the mothers increased 25% in Gr I, but only 2% in Gr II.The absorption of PI by the skin was found to lead to changes in fT3, TSH, and urine iodine excretion values in the mothers. The findings of this study of Caesarean operations, which have a short duration, might be a signal to be careful of thyroid hormone effects in operations lasting longer.
- Published
- 2013
50. The effect of centrifugation on three urine protein assays: benzethonium chloride, benzalkonium chloride and pyrogallol red
- Author
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Hatice Sürer, Gulsen Yilmaz, Fatma Yılmaz, and Tuba Özgün
- Subjects
Chromatography ,Chemistry ,Benzethonium chloride ,Pyrogallol red ,Urine total protein ,Biochemistry (medical) ,Clinical Biochemistry ,Proteins ,Centrifugation ,General Medicine ,Urine ,Pyrogallol ,Proteinuria ,Benzalkonium chloride ,chemistry.chemical_compound ,Urinary Tract Infections ,Benzethonium ,medicine ,Humans ,Artifacts ,Benzalkonium Compounds ,Urine Specimen Collection ,medicine.drug - Published
- 2014
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