1,903 results on '"Jin Yang"'
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2. Circular RNA HIPK2 Promotes A1 Astrocyte Activation after Spinal Cord Injury through Autophagy and Endoplasmic Reticulum Stress by Modulating miR-124-3p-Mediated Smad2 Repression
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Jin Yang, Junjie Dong, Haotian Li, Zhiqiang Gong, Bing Wang, Kaili Du, Chunqiang Zhang, Hangchuan Bi, Junfei Wang, Xinpeng Tian, and Lingqiang Chen
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Chemistry ,QD1-999 - Published
- 2023
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3. Construction of porphyrinic manganese-organic frameworks based on structural regulation for electrochemical determination of nitrobenzene in water and vegetable samples
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Li Wang, Mengjie Zhang, Yuanyuan Li, Xiumei Chen, Hao Qin, Jin Yang, Suhua Fan, and Hai Wu
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metal-organic frameworks ,nitrobenzene ,electrochemical sensor ,organic pollutants ,structural regulation ,Chemistry ,QD1-999 - Abstract
Nitrobenzene (NB) is one of the major organic pollutants that has seriously endangered human health and the environment even in trace amounts. Therefore, it is of great significance to detect trace NB efficiently and sensitively. Herein, a porphyrinic metal-organic framework (MOF) of Mn-PCN-222 (PCN, porous coordination network) was first synthesized by the coordination between Zr6 cluster and tetrakis (4-carboxyphenyl)-porphyrin-Mn (Ⅲ) (MnTCPPCl) ligand. To regulate its structure and the electrochemical properties, a phenyl group was inserted in each branched chain of TCPP to form the TCBPP organic ligand. Then, we used Zr6 clusters and manganese metalloporphyrin (MnTCBPPCl) to synthesize a new porphyrin-based MOF (Mn-CPM-99, CPM, crystalline porous material). Due to the extended chains of TCPP, the rod-shaped structure of Mn-PCN-222 was switched to concave quadrangular bipyramid of Mn-CPM-99. Mn-CPM-99 exhibited higher porosity, larger specific surface area, better electrochemical performances than those of Mn-PCN-222. By using modular assembly technique, Mn-CPM-99 film was sequentially assembled on the surface of indium-tin-oxide (ITO) to prepare an electrochemical sensor (Mn-CPM-99/ITO). The proposed sensor showed excellent electrochemical reduction of NB and displayed three linear response ranges in the wide concentration ranges. The obtained low limit of detection (LOD, 1.3 nM), high sensitivity and selectivity, and good reproducibility of the sensor for NB detection fully illustrate that Mn-CPM-99 is an excellent candidate for electrochemical sensor interface material. Moreover, the sensor was successfully applied to the detection of NB in lake water and vegetable samples showing satisfactory recovery of 98.9%–101.8%.
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- 2024
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4. Effect of Hole on Oil Well Cement and Failure Mechanism: Application for Oil and Gas Wells
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Zhiqiang Wu, Guangai Wu, Xuesong Xing, Jin Yang, Shujie Liu, Hao Xu, and Xiaowei Cheng
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Chemistry ,QD1-999 - Published
- 2022
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5. Experimental Study of the Impact of Chlorine Dioxide on the Permeability of High-Rank Contaminated Coal Reservoirs
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Zhou Zhang, Pengbo Zhou, Lifang Zhao, Jin Yang, Min Zhou, and Baoan Xian
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Chemistry ,QD1-999 - Published
- 2021
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6. Identification and Biological Validation of a Chemokine/Chemokine Receptor-Based Risk Model for Predicting Immunotherapeutic Response and Prognosis in Head and Neck Squamous Cell Carcinoma
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Ye Wang, Shimeng Wang, Houshang Wang, Jin Yang, and Hongmei Zhou
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head and neck squamous cell carcinoma ,chemokine and chemokine receptor ,risk model ,tumor microenvironment ,immunotherapy ,prognosis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Over 80% of head and neck squamous cell carcinoma (HNSCC) patients failed to respond to immunotherapy, which can likely be attributed to the tumor microenvironment (TME) remolding mediated by chemokines/chemokine receptors (C/CR). This study aimed to establish a C/CR-based risk model for better immunotherapeutic responses and prognosis. After assessing the characteristic patterns of the C/CR cluster from the TCGA-HNSCC cohort, a six-gene C/CR-based risk model was developed to stratify patients by LASSO Cox analysis. The screened genes were multidimensionally validated by RT-qPCR, scRNA-seq, and protein data. A total of 30.4% of patients in the low-risk group had better responses to anti-PD-L1 immunotherapy. A Kaplan–Meier analysis showed that patients in the low-risk group had longer overall survival. A time-dependent receiver operating characteristic curve and Cox analyses indicated that risk score served as an independent predictive indicator. The robustness of the immunotherapy response and prognosis prediction was also validated in independent external datasets. Additionally, the TME landscape revealed that the low-risk group was immune activated. Furthermore, the cell communication analysis on the scRNA-seq dataset revealed that cancer-associated fibroblasts were the main communicators within the C/CR ligand–receptor network of TME. Collectively, The C/CR-based risk model simultaneously predicted immunotherapeutic response and prognosis, potentially optimizing personalized therapeutic strategies of HNSCC.
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- 2023
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7. Synthesis of Acryl Group-Modified Adsorption Resins and Their Adsorption Properties for Matrine and Oxymatrine in Aqueous Solutions
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Tao Sun, Hui Li, Xiaoli Ma, Jianbao Ding, Jianlong Ma, Bolin Gong, and Jin Yang
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Chemistry ,QD1-999 - Published
- 2020
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8. New Magnetically Assembled Electrode Consisting of Magnetic Activated Carbon Particles and Ti/Sb-SnO2 for a More Flexible and Cost-Effective Electrochemical Oxidation Wastewater Treatment
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Fanxi Zhang, Dan Shao, Changan Yang, Hao Xu, Jin Yang, Lei Feng, Sizhe Wang, Yong Li, Xiaohua Jia, and Haojie Song
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anode ,metal oxide ,electrolysis ,refractory pollutant ,carbon ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
Magnetic activated carbon particles (Fe3O4/active carbon composites) as auxiliary electrodes (AEs) were fixed on the surface of Ti/Sb-SnO2 foil by a NdFeB magnet to form a new magnetically assembled electrode (MAE). Characterizations including cyclic voltammetry, Tafel analysis, and electrochemical impedance spectroscopy were carried out. The electrochemical oxidation performances of the new MAE towards different simulated wastewaters (azo dye acid red G, phenol, and lignosulfonate) were also studied. Series of the electrochemical properties of MAE were found to be varied with the loading amounts of AEs. The electrochemical area as well as the number of active sites increased significantly with the AEs loading, and the charge transfer was also facilitated by these AEs. Target pollutants’ removal of all simulated wastewaters were found to be enhanced when loading appropriate amounts of AEs. The accumulation of intermediate products was also determined by the AEs loading amount. This new MAE may provide a landscape of a more cost-effective and flexible electrochemical oxidation wastewater treatment (EOWT).
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- 2022
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9. Recent Developments in Activated Carbon Catalysts Based on Pore Size Regulation in the Application of Catalytic Ozonation
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Jin Yang, Liya Fu, Fachao Wu, Xingxing Chen, Changyong Wu, and Qibao Wang
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activated carbon ,catalytic ozonation ,mass transfer ,pore structure ,external specific surface area ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
Due to its highly developed pore structure and large specific surface area, activated carbon is often used as a catalyst or catalyst carrier in catalytic ozonation. Although the pore structure of activated carbon plays a significant role in the treatment of wastewater and the mass transfer of ozone molecules, the effect is complicated and unclear. Because different application scenarios require catalysts with different pore structures, catalysts with appropriate pore structure characteristics should be developed. In this review, we systematically summarized the current adjustment methods for the pore structure of activated carbon, including raw material, carbonization, activation, modification, and loading. Then, based on the brief introduction of the application of activated carbon in catalytic ozonation, the effects of pore structure on catalytic ozonation and mass transfer are reviewed. Furthermore, we proposed that the effect of pore structure is mainly to provide catalytic active sites, promote free radical generation, and reduce mass transfer resistance. Therefore, large external surface area and reasonable pore size distribution are conducive to catalytic ozonation and mass transfer.
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- 2022
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10. Microemulsion Synthesis of Nanosized Calcium Sulfate Hemihydrate and Its Morphology Control by Different Surfactants
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Shun Chen, Yan Xu, Xingyang He, Ying Su, Jin Yang, Wei Chen, and Hongbo Tan
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Chemistry ,QD1-999 - Published
- 2019
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11. Self-Assembled Monolayer Enables Slurry-Coating of Li Anode
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Tuo Kang, Yalong Wang, Feng Guo, Chenghao Liu, Jianghui Zhao, Jin Yang, Hongzhen Lin, Yejun Qiu, Yanbin Shen, Wei Lu, and Liwei Chen
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Chemistry ,QD1-999 - Published
- 2019
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12. Double Branch Attention Block for Discriminative Representation of Siamese Trackers
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Jiaqi Xi, Jin Yang, Xiaodong Chen, Yi Wang, and Huaiyu Cai
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object tracking ,Siamese framework ,self-attention mechanism ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Siamese trackers have achieved a good balance between accuracy and efficiency in generic object tracking. However, background distractors cause side effects to the discriminative representation of the target. To suppress the sensitivity of trackers to background distractors, we propose a Double Branch Attention (DBA) block and a Siamese tracker equipped with the DBA block named DBA-Siam. First, the DBA block concatenates channels of multiple layers from two branches of the Siamese framework to obtain rich feature representation. Second, the channel attention is applied to the two concatenated feature blocks to enhance the robust features selectively, thus enhancing the ability to distinguish the target from the complex background. Finally, the DBA block collects the contextual relevance between the Siamese branches and adaptively encodes it into the feature weight of the detection branch for information compensation. Ablation experiments show that the proposed block can enhance the discriminative representation of the target and significantly improve the tracking performance. Results on two popular benchmarks show that DBA-Siam performs favorably against its counterparts. Compared with the advanced algorithm CSTNet, DBA-Siam improves the EAO by 18.9% on VOT2016.
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- 2022
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13. Stand on the Same Side Against Covid – 19 Clinical Management of Covid-19
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Lorenzo Corbetta, Leonardo M. Fabbri, Shiyue Li, Jing Li, Bin Cao, Jin Yang, Giancarlo Agnelli, Michela Giustozzi, Alberto Mantovani, Luigi Camporota, Semra Bilaçeroğlu, and Adrian Rendon
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Covid-19 ,Clinical Management ,LOTUS China Trial ,Lopinavir ,Ritonavir ,Wuhan ,History (General) and history of Europe ,Chemistry ,QD1-999 - Abstract
This document is the direct transcription of a Webinar organized by Prof. L. Corbetta of the University of Florence on May 29th, 2020.
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- 2020
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14. Fundamental and Clinical Applications of Materials Based on Cancer-Associated Fibroblasts in Cancers
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Jingtian Mu, Shengtao Gao, Jin Yang, Fanglong Wu, and Hongmei Zhou
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cancer-associated fibroblasts ,cancer ,biochip ,three-dimensional co-cultivation ,nanomaterials ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Cancer stromal cells play a role in promoting tumor relapse and therapeutic resistance. Therefore, the current treatment paradigms for cancers are usually insufficient to eradicate cancer cells, and anti-cancer therapeutic strategies targeting stromal cells have been developed. Cancer-associated fibroblasts (CAFs) are perpetually activated fibroblasts in the tumor stroma. CAFs are the most abundant and highly heterogeneous stromal cells, and they are critically involved in cancer occurrence and progression. These effects are due to their various roles in the remodeling of the extracellular matrix, maintenance of cancer stemness, modulation of tumor metabolism, and promotion of therapy resistance. Recently, biomaterials and nanomaterials based on CAFs have been increasingly developed to perform gene or protein expression analysis, three-dimensional (3D) co-cultivation, and targeted drug delivery in cancer treatment. In this review, we systematically summarize the current research to fully understand the relevant materials and their functional diversity in CAFs, and we highlight the potential clinical applications of CAFs-oriented biomaterials and nanomaterials in anti-cancer therapy.
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- 2021
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15. Recent Development in Sludge Biochar-Based Catalysts for Advanced Oxidation Processes of Wastewater
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Xingxing Chen, Liya Fu, Yin Yu, Changyong Wu, Min Li, Xiaoguang Jin, Jin Yang, Panxin Wang, and Ying Chen
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sludge ,catalyst ,wastewater treatment ,advanced oxidation processes ,application ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
Sewage sludge as waste of the wastewater treatment process contains toxic substances, and its conversion into sludge biochar-based catalysts is a promising strategy that merges the merits of waste reutilization and environmental cleanup. This study aims to systematically recapitulate the published articles on the development of sludge biochar-based catalysts in different advanced oxidation processes of wastewater, including sulfate-based system, Fenton-like systems, photocatalysis, and ozonation systems. Due to abundant functional groups, metal phases and unique structures, sludge biochar-based catalysts exhibit excellent catalytic behavior for decontamination in advanced oxidation systems. In particular, the combination of sludge and pollutant dopants manifests a synergistic effect. The catalytic mechanisms of as-prepared catalysts in these systems are also investigated. Furthermore, initial solution pH, catalyst dosage, reaction temperature, and coexisting anions have a vital role in advanced oxidation processes, and these parameters are systematically summarized. In summary, this study could provide relatively comprehensive and up-to-date messages for the application of sludge biochar-based catalysts in the advanced oxidation processes of wastewater treatment.
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- 2021
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16. Cross Subaperture Averaging Generalized Sidelobe Canceler Beamforming Applied to Medical Ultrasound Imaging
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Jin Yang, Jiake Li, Xiaodong Chen, Jiaqi Xi, Huaiyu Cai, and Yi Wang
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GSC-CROSS ,adaptive beamforming ,minimum variance ,covariance matrix ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
For adaptive ultrasound imaging, a reliable estimation of the covariance matrix has a decisive influence on the performance of beamformers. In this paper, we propose a new cross subaperture averaging generalized sidelobe canceler approach (GSC-CROSS) for medical ultrasound imaging, which uses the cross-covariance matrix instead of the traditional covariance matrix estimation. By using the more stable and accurate estimation of the covariance matrix, GSC-CROSS performs well in both lateral resolution and contrast. Experiments are conducted based on the simulated echo data of scattering points and a cyst target. Beamforming responses of scattering points show that GSC-CROSS can improve the lateral resolution by 76.9%, 68.8%, and 17.1% compared with delay-and-sum (DS), synthetic aperture (SA), and the traditional generalized sidelobe canceler (GSC), respectively. Also, imaging of the cyst target shows that compared with DS, SA, and GSC, the contrast increases by 101%, 32.6%, and 63.5%, respectively. Finally, the actual echo data collected from a medical ultrasonic imaging system is applied to reconstruct the image. Results show that the proposed method has a good performance on lateral resolution and contrast. Both the simulated and experimental data demonstrate the effectiveness of the proposed method.
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- 2021
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17. Experimental Investigation and Molecular Dynamics Simulation on the Anti-Adhesion Behavior of Alkanethiols on Nickel Insert in Micro Injection Molding
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Can Weng, Jiachen Chen, Jin Yang, Mingyong Zhou, and Bingyan Jiang
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anti-adhesion ,self-assembled monolayers ,molecular dynamics simulation ,micro injection molding ,Chemistry ,QD1-999 - Abstract
Due to the adhesion between the polymer melt and nickel (Ni) mold insert in the micro injection molding process, deformation defects frequently occur when the microstructures are demolded from the insert. In this study, self-assembled alkanethiols were applied to modify the surface of Ni mold insert to reduce its surface energy. Experimental trials were undertaken to explore the effect of alkanethiols coating on the replication quality. After that, molecular dynamics (MD) simulation was then used to investigate the adhesion behavior between the self-assembled coating and polypropylene (PP) by establishing three different types of alkanethiol material. The interaction energy, the potential energy change and radial distribution function were calculated to study the anti-adhesion mechanism. Experimental results show that all the three coatings can effectively decrease the adhesion and therefore promote the replication fidelity. It is demonstrated in MD simulation that the adhesion mainly comes from the van der Waals (vdW) force at the interface. The arrangement of sulfur atom on the Ni surface results in different absorbing behaviors. Compared with that of the PP–Ni interface, the interfacial energy and adhesion work after surface treatment is significantly reduced.
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- 2021
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18. Wet process of phosphoric acid purification by solvent extraction using tri-n-butyl phosphate and cyclohexanol mixtures
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Li Xing, Li Jun, Jin Yang, Chen Ming, Feng Dongya, and Guo Yunhai
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mixing extractant ,equilibrium phase diagram ,extracting mechanism ,reciprocating plate column ,Chemistry ,QD1-999 - Abstract
Solvent extraction is an efficient, economical and widely-used technology for the purification of wet process phosphoric acid (WPA). This present work focused on the development of a solvent extraction system, TC, representing tri-n-butyl phosphate (TBP) and cyclohexanol mixtures. The equilibrium phase diagram of the system H3PO4−H2O−TC at 298.2 K and atmospheric pressure was obtained. The effects of extraction time, phosphoric acid concentration, extractant concentration, temperature and phase volume ratio on extraction efficiency were studied. The extracted complexes were estimated to be 1.8H3PO4 2TC. The extraction process of H3PO4 was exothermic and the enthalpy change, ΔH, was obtained. The solvent mixtures had a high efficiency for phosphoric acid purification via multi-stage counter-current extraction from the industrial WPA in a reciprocating plate extraction column with an H3PO4 extraction yield of 85.08 % at 313.2 K. The stability, extraction efficiency and recycling capability of TC for H3PO4 extraction were also estimated.
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- 2017
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19. Genome-Wide Survey and Expression Analysis of the KT/HAK/KUP Family in Brassica napus and Its Potential Roles in the Response to K+ Deficiency
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Jie Zhou, Hong-Jun Zhou, Ping Chen, Lan-Lan Zhang, Jia-Tian Zhu, Peng-Feng Li, Jin Yang, Yun-Zhuo Ke, Yong-Hong Zhou, Jia-Na Li, and Hai Du
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Brassica napus ,potassium transporter ,KT/HAK/KUP family ,evolution ,expression analysis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The KT/HAK/KUP (HAK) family is the largest potassium (K+) transporter family in plants, which plays key roles in K+ uptake and homeostasis, stress resistance, and root and embryo development. However, the HAK family has not yet been characterized in Brassica napus. In this study, 40 putative B. napus HAK genes (BnaHAKs) are identified and divided into four groups (Groups I–III and V) on the basis of phylogenetic analysis. Gene structure analysis revealed 10 conserved intron insertion sites across different groups. Collinearity analysis demonstrated that both allopolyploidization and small-scale duplication events contributed to the large expansion of BnaHAKs. Transcription factor (TF)-binding network construction, cis-element analysis, and microRNA prediction revealed that the expression of BnaHAKs is regulated by multiple factors. Analysis of RNA-sequencing data further revealed extensive expression profiles of the BnaHAKs in groups II, III, and V, with limited expression in group I. Compared with group I, most of the BnaHAKs in groups II, III, and V were more upregulated by hormone induction based on RNA-sequencing data. Reverse transcription-quantitative polymerase reaction analysis revealed that the expression of eight BnaHAKs of groups I and V was markedly upregulated under K+-deficiency treatment. Collectively, our results provide valuable information and key candidate genes for further functional studies of BnaHAKs.
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- 2020
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20. Rational Regulation of Surface Free Radicals on TiO2 Nanotube Arrays via Ag2O–AgBiO3 towards Enhanced Selective Photoelectrochemical Detection
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Yajun Pang, Hao Chen, Jin Yang, Bo Wang, Zhenyu Yang, Jun Lv, Zhenghui Pan, Guangqing Xu, Zhehong Shen, and Yucheng Wu
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TiO2 nanotube arrays ,AgBiO3 ,precipitation ,high selectivity ,photoelectrochemical sensors ,organics detection ,Chemistry ,QD1-999 - Abstract
Due to integrated advances in photoelectrochemical (PEC) functionalities for environment detection applications, one-dimensional (1D) TiO2 nanostructures provide a new strategy (PEC sensors) towards organics detection in wastewater. However, the unidealized selectivity to the oxidation of water and organics limits the PEC detection performance. Herein, we designed a ternary photoanode consisting of Ag2O–AgBiO3/TiO2 nanotube arrays (NTAs) to solve this issue by using a facile one-step precipitation reaction. High oxidation capacity for organics is achieved by regulating the surface free radicals properly through the heterostructure formed between the interface of TiO2 and AgBiO3. More importantly, as a trap for electron capture, Ag2O in this ternary system could not only further improve the separation efficiency of charge carriers, but also capture electrons transferred to the TiO2 conduction band, thus reducing the electrons transferred to the external circuit and the corresponding background photocurrent when detecting organics. As a result, the reconstructed TiO2 NTAs decrease their photocurrent response to water and enhance their response to organics, thus presenting lower oxidation activity to water and higher activity to organics, that is, highly selective oxidation characteristics. This work provides more insights into the impact of charge transfer and surface free radicals on developing promising and efficient PEC sensors for organics.
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- 2020
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21. MiR-581/SMAD7 Axis Contributes to Colorectal Cancer Metastasis: A Bioinformatic and Experimental Validation-Based Study
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Xiaojuan Zhao, Shuzhen Liu, Bianbian Yan, Jin Yang, and Erfei Chen
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colorectal cancer ,microRNA ,bioinformatics ,signaling pathway ,metastasis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Metastasis is a well-known poor prognostic factor and primary cause of mortality in patients with colorectal cancer (CRC). Recently, with the progress of high through-put sequencing, aberrantly expressed non-coding RNAs (ncRNAs) were found to participate in the initiation and development of cancer. However, the mechanisms of ncRNA-mediated regulation of metastasis in CRC remain largely unknown. In this study, we systematically analyzed the expression network of microRNAs (miRNAs) and genes in CRC metastasis using bioinformatics, and discovered that the miR-581/SMAD7 axis could be a potential factor that drives CRC metastasis. A dual luciferase report assay and protein analysis confirmed the binding relationship between miR-581 and SMAD7. Further functional assays revealed that miR-581 inhibition could suppress cell proliferation and induce apoptosis in SW480 cells. Up-regulation or down-regulation of miR-581 could both affect cell invasion capacity and modulate epithelial to mesenchymal transition (EMT) via a SMAD7/TGFβ signaling pathway. In conclusion, our findings elucidated that miR-581/SMAD7 could be essential for CRC metastasis, and may serve as a potential therapeutic target for CRC patients.
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- 2020
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22. Preparation of Gd-Doped TiO2 Nanotube Arrays by Anodization Method and Its Photocatalytic Activity for Methyl Orange Degradation
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Rui Li, Jin Yang, Shiyin Xu, Yushun Zhou, Xuan Wang, Hailong Peng, and Jun Du
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gd-doped tio2 ,nanotube arrays ,anodization ,photocatalysis ,hydrophilicity ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
Gd-doped TiO2 nanotube arrays with 3D ordered and high specific surface (176 m2/g) area are successfully prepared on a Ti foil surface via an anodizing method. The characterizations of Gd-doped TiO2 nanotube arrays are carried out using X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy (XPS), energy dispersive X-ray spectrometer (EDX), optical contact angle measurer, and ultraviolet (UV) fluorescence spectrophotometer, respectively. The results reveal that the Gd-doped TiO2 nanotube arrays form on the Ti foil surface with a length of about 50.5 μm and 100 nm in diameter, and the growth direction grown along the (101) direction of anatase crystal. Under the optimized reaction conditions (60 V, 10 h, 0.01mol/L Gd3+), the crystal phase of Gd-doped TiO2 show an excellent nanotube structure, possess a beneficial photocatalytic performance for methyl orange (MO) (Degradation rate was 95.8%) and an excellent hydrophilic property (The optical contact angle was 4.9°). After doping with Gd, the hydrophilic and photocatalytic properties of the TiO2 nanotubes are further improved.
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- 2020
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23. Global Survey and Expressions of the Phosphate Transporter Gene Families in Brassica napus and Their Roles in Phosphorus Response
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Jin Yang, Jie Zhou, Hong-Jun Zhou, Mang-Mang Wang, Ming-Ming Liu, Yun-Zhuo Ke, Peng-Feng Li, Jia-Na Li, and Hai Du
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brassica napus l. ,pht gene family ,phylogenetic analysis ,expression analysis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Phosphate (Pi) transporters play critical roles in Pi acquisition and homeostasis. However, currently little is known about these genes in oil crops. In this study, we aimed to characterize the five Pi transporter gene families (PHT1-5) in allotetraploid Brassica napus. We identified and characterized 81 putative PHT genes in B. napus (BnaPHTs), including 45 genes in PHT1 family (BnaPHT1s), four BnaPHT2s, 10 BnaPHT3s, 13 BnaPHT4s and nine BnaPHT5s. Phylogenetic analyses showed that the largest PHT1 family could be divided into two groups (Group I and II), while PHT4 may be classified into five, Groups I-V. Gene structure analysis revealed that the exon-intron pattern was conservative within the same family or group. The sequence characteristics of these five families were quite different, which may contribute to their functional divergence. Transcription factor (TF) binding network analyses identified many potential TF binding sites in the promoter regions of candidates, implying their possible regulating patterns. Collinearity analysis demonstrated that most BnaPHTs were derived from an allopolyploidization event (~40.7%) between Brassica rapa and Brassica oleracea ancestors, and small-scale segmental duplication events (~39.5%) in the descendant. RNA-Seq analyses proved that many BnaPHTs were preferentially expressed in leaf and flower tissues. The expression profiles of most colinearity-pairs in B. napus are highly correlated, implying functional redundancy, while a few pairs may have undergone neo-functionalization or sub-functionalization during evolution. The expression levels of many BnaPHTs tend to be up-regulated by different hormones inductions, especially for IAA, ABA and 6-BA treatments. qRT-PCR assay demonstrated that six BnaPHT1s (BnaPHT1.11, BnaPHT1.14, BnaPHT1.20, BnaPHT1.35, BnaPHT1.41, BnaPHT1.44) were significantly up-regulated under low- and/or rich- Pi conditions in B. napus roots. This work analyzes the evolution and expression of the PHT family in Brassica napus, which will help further research on their role in Pi transport.
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- 2020
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24. In-Depth Understanding of Camellia oleifera Self-Incompatibility by Comparative Transcriptome, Proteome and Metabolome
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Junqin Zhou, Mengqi Lu, Shushu Yu, Yiyao Liu, Jin Yang, and Xiaofeng Tan
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oil-tea ,camellia oleifera ,mitogen-activated protein kinase ,plant hormone ,pollen tube death ,programmed cell death ,ubiquitination ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Oil-tea tree (Camellia oleifera) is the most important edible oil tree species in China with late-acting self-incompatibility (LSI) properties. The mechanism of LSI is uncertain, which seriously hinders the research on its genetic characteristics, construction of genetic map, selection of cross breeding parents and cultivar arrangement. To gain insights into the LSI mechanism, we performed cytological, transcriptomic, proteomic and metabolomic studies on self- and cross-pollinated pistils. The studies identified 166,591 transcripts, 6851 proteins and 6455 metabolites. Transcriptomic analysis revealed 1197 differentially expressed transcripts between self- and cross-pollinated pistils and 47 programmed cell death (PCD)-control transcripts. Trend analysis by Pearson correlation categorized nine trend graphs linked to 226 differentially expressed proteins and 38 differentially expressed metabolites. Functional enrichment analysis revealed that the LSI was closely associated with PCD-related genes, mitogen-activated protein kinase (MAPK) signaling pathway, plant hormone signal transduction, ATP-binding cassette (ABC) transporters and ubiquitin-mediated proteolysis. These particular trends in transcripts, proteins and metabolites suggested the involvement of PCD in LSI. The results provide a solid genetic foundation for elucidating the regulatory network of PCD-mediated self-incompatibility in C. oleifera.
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- 2020
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25. Circulating Long Noncoding RNA as a Potential Target for Prostate Cancer
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Yin-Jie Su, Jin Yu, Ya-Qin Huang, and Jin Yang
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long noncoding RNA ,prostate cancer ,biomarker ,therapeutic target ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Prostate cancer is considered the second most common visceral malignancy in men in Western countries. Its emergence is largely due to the coordination of a malignant network, and long noncoding RNA has been recently demonstrated to play a critical role in prostate carcinogenesis. The aberrant expression of long noncoding RNA in prostate cancer patients is strongly associated with diagnosis, risk stratification and carcinogenesis, information that provides new insight into the complicated intracellular milieu of prostate cancer. This review focuses mainly on literature evidence for the role of long noncoding RNA in prostate cancer, which may suggest novel strategies for its prognosis, diagnosis and clinical treatment.
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- 2015
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26. Preparation and Evaluation of Human-Murine Chimeric Antibody against Protective Antigen of Bacillus anthracis
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Lina Hao, Feng Zheng, Siping Xiong, Dan Hu, Heng Lv, Qi Tang, Jin Yang, Zhenqing Feng, Changjun Wang, and Jin Zhu
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chimeric Fab antibody ,anthrax ,PA ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The aim of this research is to develop a human/murine chimeric Fab antibody which neutralizes the anthrax toxin, protective antigen (PA). The chimeric Fab was constructed using variable regions of murine anti-PA monoclonal antibody in combination with constant regions of human IgG. The chimeric PA6-Fab was expressed in E. coli. BL21 and evaluated by ELISA and co-immunoprecipitation- mass spectra. The potency of PA6-Fab to neutralize LeTx was examined in J774A.1 cell viability in vitro and in Fisher 344 rats in vivo. The PA6-Fab did not have domain similarity corresponding to the current anti PA mAbs, but specifically bound to anthrax PA at an affinity of 1.76 nM, and was able to neutralize LeTx in vitro and protected 56.9% cells at 20 μg/mL against anthrax LeTx. One hundred μg PA6-Fab could neutralize 300 μg LeTx in vivo. The PA6-Fab has potential as a therapeutic mAb for treatment of anthrax.
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- 2014
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27. Indium Tin-Oxide Wrapped 3D rGO and TiO2 Composites: Development, Characterization, and Enhancing Photocatalytic Activity for Methylene Blue
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Cheng Gong, Shiyin Xu, Peng Xiao, Feifan Liu, Yunhui Xu, Jin Yang, Rui Li, Xuan Wang, Jun Du, and Hailong Peng
- Subjects
ito-rgo and tio2 ,methylene blue ,photocatalysis ,adsorption ,mechanisms ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
A hybrid material of indium tin-oxide (ITO) wrapped titanium dioxide and reduced graphene oxide (ITO-rGO and TiO2) was prepared using a facile hydrothermal technique. TiO2 nanorods were in situ grown on the surface of rGO (rGO and TiO2), and which was then assembled onto ITO substrate layer by layer with formation of a 3D structure. ITO-rGO and TiO2 exhibit low charge transfer resistance at the electrode-electrolyte interface and have good photoresponsive ability. Methylene blue (MB) can be effectively adsorbed and enriched onto ITO-rGO and TiO2 surface. The adsorption kinetics and thermodynamics of ITO-rGO and TiO2 were evaluated, showing that the exothermic and entropy-driven reaction were the main thermodynamic processes, and the Langmuir isotherm was the ideal model for adsorption fitting. Meanwhile, ITO greatly improved degradation of rGO and TiO2 because electrons can be collected by ITO before recombination and MB can easily enter into the 3D structure of rGO and TiO2. The highest photodegradation rate of MB reached 93.40% for ITO-rGO and TiO2 at pH 9. Additionally, ITO-rGO and TiO2 successfully solved the problems of being difficult to recycle and causing secondary pollution of traditional TiO2 catalysts. Therefore, ITO-rGO and TiO2 may be a potential photocatalyst for degrading organic pollutants in water.
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- 2019
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28. Evolutionary and Comparative Expression Analyses of TCP Transcription Factor Gene Family in Land Plants
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Ming-Ming Liu, Mang-Mang Wang, Jin Yang, Jing Wen, Peng-Cheng Guo, Yun-Wen Wu, Yun-Zhuo Ke, Peng-Feng Li, Jia-Na Li, and Hai Du
- Subjects
TCP transcription factor ,phylogenetic analysis ,origin ,evolution ,interaction network ,expression profile analysis ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The plant-specific Teosinte-branched 1/Cycloidea/Proliferating (TCP) transcription factor genes are involved in plants’ development, hormonal pathways, and stress response but their evolutionary history is uncertain. The genome-wide analysis performed here for 47 plant species revealed 535 TCP candidates in terrestrial plants and none in aquatic plants, and that TCP family genes originated early in the history of land plants. Phylogenetic analysis divided the candidate genes into Classes I and II, and Class II was further divided into CYCLOIDEA (CYC) and CINCINNATA (CIN) clades; CYC is more recent and originated from CIN in angiosperms. Protein architecture, intron pattern, and sequence characteristics were conserved in each class or clade supporting this classification. The two classes significantly expanded through whole-genome duplication during evolution. Expression analysis revealed the conserved expression of TCP genes from lower to higher plants. The expression patterns of Class I and CIN genes in different stages of the same tissue revealed their function in plant development and their opposite effects in the same biological process. Interaction network analysis showed that TCP proteins tend to form protein complexes, and their interaction networks were conserved during evolution. These results contribute to further functional studies on TCP family genes.
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- 2019
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29. Highly Hydrophilic TiO2 Nanotubes Network by Alkaline Hydrothermal Method for Photocatalysis Degradation of Methyl Orange
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Jin Yang, Jun Du, Xiuyun Li, Yilin Liu, Chang Jiang, Wenqian Qi, Kai Zhang, Cheng Gong, Rui Li, Mei Luo, and Hailong Peng
- Subjects
titanium dioxide ,nanotubes network ,photocatalysis ,alkaline hydrothermal method ,hydrophilicity ,Chemistry ,QD1-999 - Abstract
High-density and highly cross-coated anatase TiO2 nanotubes networks have been successfully prepared on the surface of Ti foil by alkaline hydrothermal using NaOH and Ti foil as the precursors. The nanotubes networks were analyzed using X-ray diffraction (XRD), energy dispersive X-ray spectrometer (EDX), transmission electron microscope (TEM), scanning electron microscopy (SEM), optical contact angle tester, and ultraviolet (UV) fluorescence spectrophotometer, respectively. The results showed that the nanotubes network with diameters of 30–50 nm were obtained on the Ti foil surface. The morphology of the nanotubes network possessed the three-dimensional network structure, The TiO2 nanotubes network grew along the (101) direction of the tetragonal anatase crystal. The morphology and crystal phase of the TiO2 nanotubes network were better at the conditions of NaOH concentration 7–10 mol/L and temperature 160–170 °C. The best contact angle of TiO2 nanotubes network after UV-light irradition was only 5.1 ± 2.9°. Under the irradiation of mercury lamp, the nanotubes network exhibited excellent photocatalytic performance and the degradation ratio of methyl orange solution reached to 80.00 ± 2.33%. Thus, the anatase TiO2 nanotubes network has great potential in applications for pollution photocatalytic degradation.
- Published
- 2019
- Full Text
- View/download PDF
30. Interface Growth and Void Formation in Sn/Cu and Sn0.7Cu/Cu Systems
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Jieshi Chen, Yongzhi Zhang, Zhishui Yu, Peilei Zhang, Wanqin Zhao, Jin Yang, and Di Wu
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Sn/Cu solder joint ,intermetallic compounds ,void ,electroplated Cu ,Cu addition ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
In this work, the effects of electroplated Cu (EP Cu) and Cu addition (0.7%) in Sn solder on the intermetallic compounds (IMCs) growth and void formation were clarified by comparison with solder joints comprising of high purity Cu (HP Cu) substrate and pure Sn solder. After aging processes, a new IMC, Cu3Sn, was formed at the interface, in addition to Cu6Sn5 formed in the as-soldered joints. The EP Cu and Cu addition (0.7%) both had limited effects on the total IMCs thickness. However, the effects varied on the growth behaviors of different IMCs. Comparing to the void-free interface between Sn and HP Cu, a large number of voids were observed at the Cu3Sn/Cu interface in Sn/EP Cu joints. The formation of these voids may be induced by the impurities and fine grain, which were introduced during the electroplating process. The addition of Cu suppressed the inter-diffusion of Cu and Sn at the interface. Consequently, the growth of the Cu3Sn layer and formation of voids were suppressed.
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- 2018
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31. Transformation of 2-Butene into Propene on WO3/MCM-48: Metathesis and Isomerization of n-Butene
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Derun Hua, Zheng Zhou, Qianqian Hua, Jian Li, Xinning Lu, Yongrong Xie, Hong Xiao, Mei Li, and Jin Yang
- Subjects
MCM-48 mesoporous ,olefin metathesis ,propene ,WO3 ,Chemical technology ,TP1-1185 ,Chemistry ,QD1-999 - Abstract
The metathesis of 2-butene (Trans and Cis) to propene was investigated over W-based catalysts. Thermodynamic calculations for metathesis and isomerization were carried out at various temperatures to test the reactions. The results showed that the WO3/MCM-48 catalyst had good catalytic activity. The metathesis activity depended on the acidity of the catalyst and the dispersity of the WO3 on the supports. High temperatures promoted the isomerization of 2-butene to 1-butene. According to thermodynamic analysis, however, this is adverse to the metathesis reaction, making it important to determine an appropriate reaction temperature.
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- 2018
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32. Quantitative Structure-Activity Relationship Studies on Indenoisoquinoline Topoisomerase I Inhibitors as Anticancer Agents in Human Renal Cell Carcinoma Cell Line SN12C
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Jin Yang, Shengchao Tian, Fang Yuan, Yang Liu, Yi Zhang, Pinghua Sun, Bo Song, Zhiwen Chen, and Yi Zhi
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CoMFA ,CoMSIA ,QSAR ,indenoisoquinoline ,Top1 inhibitors ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Topoisomerase I is important for DNA replication and cell division, making it an attractive drug target for anticancer therapy. A series of indenoisoquinolines displaying potent Top1 inhibitory activity in human renal cell carcinoma cell line SN12C were selected to establish 3D-QSAR models using CoMFA and CoMSIA methods. Internal and external cross-validation techniques were investigated, as well as some measures taken, including region focusing, bootstrapping and the “leave-group-out” cross-validation method. The satisfactory CoMFA model predicted a q2 value of 0.659 and an r2 value of 0.949, indicating that electrostatic and steric properties play a significant role in potency. The best CoMSIA model, based on a combination of steric, electrostatic and H-bond acceptor descriptors, predicted a q2 value of 0.523 and an r2 value of 0.902. The models were graphically interpreted by contour plots which provided insight into the structural requirements for increasing the activity of a compound, providing a solid basis for future rational design of more active anticancer agents.
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- 2012
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33. One-Step Acidic Hydrothermal Preparation of Dendritic Rutile TiO2 Nanorods for Photocatalytic Performance
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Cheng Gong, Jun Du, Xiuyun Li, Zhenjie Yu, Jiansong Ma, Wenqian Qi, Kai Zhang, Jin Yang, Mei Luo, and Hailong Peng
- Subjects
titanium dioxide ,nanorods ,photocatalysis ,acidic hydrothermal method ,hydrophilicity ,Chemistry ,QD1-999 - Abstract
Three-dimensional and dendritic rutile TiO2 nanorods were successfully fabricated on a Ti foil surface using a one-step acidic hydrothermal method. The TiO2 nanorods were characterized using X-ray diffraction (XRD), energy dispersive X-ray spectrometry (EDX), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and optical contact angle testing. The results showed that the nanorods with diameters of 100–500 nm and lengths of 100 nm to 1 μm were obtained on the Ti foil surface. The length and density of the TiO2 nanorods were perfect at the conditions of HCl concentration 0.5 mol/L, temperature 220 °C, and reaction time 12 h. The TiO2 nanorods formed parallel to the consumption of Ti and grew along the (110) direction having a tetragonal rutile crystal. The morphology of the nanorods possessed a three-dimensional structure. The contact angle of the nanorods was only 13 ± 3.1°. Meanwhile, the photocatalytic activities of the TiO2 nanorods were carried out using ultraviolet fluorescence spectrophotometry for the methyl orange detection, and the degradation was found to be about 71.00% ± 2.43%. Thus, TiO2 nanorods can be developed by a one-step acidic hydrothermal method using Ti foil simultaneously as the substrate with a TiO2 source; the TiO2 nanorods exhibited photocatalytic performance while being environment-friendly.
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- 2018
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34. Diode Laser Welding/Brazing of Aluminum Alloy to Steel Using a Nickel Coating
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Jin Yang, Jieshi Chen, Wanqin Zhao, Peilei Zhang, Zhishui Yu, Yulong Li, Zhi Zeng, and Norman Zhou
- Subjects
laser welding/brazing ,aluminum alloy ,Ni coating ,interfacial microstructure ,intermetallic compounds ,mechanical properties ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Joining Al alloy to steel is of great interest for application in the automotive industry. Although a vast number of studies have been conducted to join Al to steel, the joining of Al to steel is still challenging due to the formation of brittle Fe–Al intermetallic compounds. In this work, the microstructure and mechanical properties of the dissimilar Al/steel joints with and without a nickel coating are comparatively investigated. A homogenous reaction layer composed of FeZn10 and Fe2Al5 is formed at the interface in the joints without Ni coating, and the joint facture load is only 743 N. To prevent the formation of brittle Fe2Al5, Ni electroplated coating is applied onto a steel surface. It has been shown that a nonhomogeneous reaction layer is observed at the interfacial region: Ni5Zn21 is formed at the direct irradiation zone, while Al3Ni is formed at the fusion zone root. The microhardness of the interfacial layer is reduced, which leads to the improvement of the joint mechanical properties. The average fracture load of the Al/Ni-coated steel joints reaches 930 N. In all of the cases, failure occurs at the Ni coating/fusion zone interface.
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- 2018
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35. Mesoporous poly(ionic liquid)s with dual active sites for highly efficient CO2 conversion
- Author
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Hong Yanzhen, Zepeng Zeng, Yanan Xu, Su Yuzhong, Li Peng, Yawen Fu, Jin Yang, Wendy L. Queen, Wang Yanliang, Shuliang Yang, Yaqiang Xie, Jun Li, Hongtao Wang, and Abdul-Rauf Ibrahim
- Subjects
Renewable Energy, Sustainability and the Environment ,Metalation ,Substrate (chemistry) ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Chemical reaction ,0104 chemical sciences ,Catalysis ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Ionic liquid ,Chemical stability ,Epichlorohydrin ,0210 nano-technology ,Mesoporous material - Abstract
Atmospheric CO2 concentrations are soaring due to the continued use of fossil fuels in energy production, an anthropogenic activity that is playing a leading role in global warming. Thus, research aimed at the capture and conversion of CO2 into value-added products, such as cyclic carbonates, is booming. While CO2 is an abundant, cheap, non-toxic, and readily accessible C1 feedstock, its thermodynamic stability necessitates the development of highly efficient catalysts that are able to promote chemical reactions under mild conditions. In this work, a novel mesoporous poly(ionic liquid) with dual active sites was synthesized through a facile method that involves co-polymerization, post-synthetic metalation, and supercritical CO2 drying. Due to a high density of nucleophilic and electrophilic sites, the as-prepared poly(ionic liquid), denoted as P2D-4BrBQA-Zn, offers excellent performance in a CO2 cycloaddition reaction using epichlorohydrin as the substrate (98.9% conversion and 96.9% selectivity). Moreover the reaction is carried out under mild, solvent-free, and additive-free conditions. Notably, P2D-4BrBQA-Zn also efficiently promotes the conversion of various other epoxide substrates into cyclic carbonates. Overall, the catalyst is found to have excellent substrate compatibility, stability, and recyclability.
- Published
- 2023
36. New Staudinger Strategy Enabled N -Acyl Phosphinamidites Synthesis
- Author
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Shang-Dong Yang, Jin Yang, Gang-Wei Wang, and Yuan-Yuan Zhu
- Subjects
Chemistry ,Stereochemistry ,lipids (amino acids, peptides, and proteins) ,General Chemistry ,Bond cleavage - Abstract
A new Staudinger strategy entails a unique C–P bond cleavage, instead of N–P bond cleavage, during the construction of N-acyl phosphinamidites by using acyl-phosphine substrates. The reaction is pe...
- Published
- 2022
37. Sphingosine-1-phosphate, a novel TREM2 ligand, promotes microglial phagocytosis to protect against ischemic brain injury
- Author
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Jin Yang, Juan Ji, Zhen-Yu Cai, Yu-Qin Sun, Hong Cheng, Xiu-Lan Sun, Wei Guo, Teng-Fei Xue, Xin-Xin Huang, and Ruo-Bing Guo
- Subjects
Apolipoprotein E ,Microglia ,Chemistry ,TREM2 ,Phagocytosis ,Ligand (biochemistry) ,Cell biology ,chemistry.chemical_compound ,medicine.anatomical_structure ,LDL receptor ,medicine ,lipids (amino acids, peptides, and proteins) ,Sphingosine-1-phosphate ,General Pharmacology, Toxicology and Pharmaceutics ,Receptor - Abstract
The mechanism of sphingosine-1-phosphate (S1P)-mediated phagocytosis remains unknown. Here, we found that S1P or FTY720 (an analog of S1P) promoted microglial phagocytosis in stroke independent of S1PRs. First, we used computer simulation of molecular docking to predict that S1P might be a ligand for triggering receptor expressed on myeloid cells 2 (TREM2). Next, microscale thermophoresis (MST), surface plasmon resonance (SPR) and liquid chromatography–tandem mass spectrometry (LC–MS/MS) were performed to reveal that S1P was a novel TREM2 ligand. Then, we confirmed the pro-phagocytosis of S1P targeting in Trem2-Dap12 transfected CHO cells and TREM2 knockdown microglia. Point mutation analysis showed that D104 was the critical binding residue. Trem2–/– mice were used to demonstrate the role of S1P-induced phagocytosis targeting on TREM2 in protecting against ischemic brain injury. Finally, further studies revealed that apolipoprotein E (APOE) loaded with S1P was released by microglia and bound to apoptotic neurons via LDL receptor related protein 1B (LRP1B) and thereby induced microglia to phagocytose apoptotic neurons. Overall, the present work reveals for the first time that S1P acts as a novel endogenous ligand of TREM2 to effectively promote microglial phagocytosis. Our findings provide a new lead compound for developing immunomodulator targeting on TREM2.
- Published
- 2022
38. Electrochemical regioselective synthesis of N-substituted/unsubstituted 4-selanylisoquinolin-1(2H)-ones
- Author
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Zhuo-Tao Zhang, Zhi-Lin Wu, Jin-Yang Chen, Xian-Zhi Tian, Wen-Tao Ouyang, and Wei-Min He
- Subjects
Chemistry ,Regioselectivity ,General Chemistry ,Electrochemistry ,Combinatorial chemistry - Abstract
A novel and efficient electro-chemical initiated radical strategy was developed for the preparation of both N-substituted and N-unsubstituted 4-selanylisoquinolin-1(2H)-ones through selenylation of isoquinolin-1(2H)-ones with organodiselenides under chemical oxidant-, additive-free and ambient conditions.
- Published
- 2022
39. Effect of organic alkali on hydration of GGBS-FA blended cementitious material activated by sodium carbonate
- Author
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Junjie Zhang, Nie Kangjun, Huiyong Guo, Jin Yang, Xiufeng Deng, Xingyang He, Yingbin Wang, and Hongbo Tan
- Subjects
Cement ,Materials science ,Process Chemistry and Technology ,Triisopropanolamine ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Compressive strength ,chemistry ,Ground granulated blast-furnace slag ,Materials Chemistry ,Ceramics and Composites ,Pozzolanic reaction ,Cementitious ,Leaching (metallurgy) ,Sodium carbonate ,Nuclear chemistry - Abstract
Sodium carbonate (SC) activated ground granulated blast-furnace slag (GGBS) and fly ash (FA) is a potential substitute of traditional cement with ultra-low carbon footprint. However, its hydration rate and strength growth are limited, owing to the slow leaching of ions during the activation of weak base. In this work, 60% wet-grinded GGBS and 40% FA were blended as the binder, and two organic alkalis, i.e. triethanolamine (TEA) and triisopropanolamine (TIPA), were adopted to facilitate the ions dissolution and pozzolanic reaction of SC activated GGBS-FA blended cementitious material (SCSF). The compressive strength was tested and the hydration kinetics was studied by hydration heat and chemical shrinkage. Besides, ions leaching behavior was characterized by ICP; hydrates and microstructure were also detected by XRD, TG-DTG and SEM. Results indicated that the addition of TEA or TIPA significantly promoted the ions dissolution. 0.05% TEA increased the concentration of leached Ca2+, Al3+ and Fe3+ by 36%, 33% and 1545%, respectively. This solubilizing effect was also found in TIPA. Moreover, these two chemicals could promote the formation of hydrates, such as C-S(A)-H gel, hydrotalcite, calcite and aragonite, especially at the early period; these also activated the hydration process of SCSF effectively, and the compressive strength of the mortar reached above 40.0 MPa at 7 d and 48.0 MPa at 28 d. The carbon emission of the designed system is 85.9% and 55.9% lower than that of PC and strong base activated GGBS system, respectively.
- Published
- 2022
40. Promote the conductivity of solid polymer electrolyte at room temperature by constructing a dual range ionic conduction path
- Author
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Ruiyang Li, Zhiqiang Chen, Haiming Hua, Peng Zhang, Jin Yang, Jinbao Zhao, and Yuejing Zeng
- Subjects
chemistry.chemical_classification ,Materials science ,Energy Engineering and Power Technology ,Ionic bonding ,02 engineering and technology ,Polymer ,Electrolyte ,Conductivity ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Thermal conduction ,01 natural sciences ,0104 chemical sciences ,Amorphous solid ,Crystal ,Fuel Technology ,chemistry ,Chemical engineering ,Electrochemistry ,Ionic conductivity ,0210 nano-technology ,Energy (miscellaneous) - Abstract
Poly(ethylene oxide) (PEO) is a classic matrix model for solid polymer electrolyte which can not only dissociate lithium-ions (Li+), but also can conduct Li+ through segmental motion in long-range. However, the crystal aggregation state of PEO restricts the conduction of Li+ especially at room temperature. In this work, an amorphous polymer electrolyte with ethylene oxide (EO) and propylene oxide (PO) block structure (B-PEG@DMC) synthesized by the transesterification is firstly obtained, showing an ionic conductivity value of 1.1 × 10−5 S/cm at room temperature (25 °C). According to the molecular dynamics (MD) simulation, the PO segments would lead to an inconsecutive and hampered conduction of Li+, which is not beneficial to the short range conduction of Li+. Thus the effect of transformation of aggregation state on the improvement of ionic conductivity is not enough, it is necessary to further consider the different coupled behaviours of EO and PO segments with Li+. In this way, we blend this amorphous polymer (B-PEG@DMC) with PEO to obtain a dual range ionic conductive solid polymer electrolyte (D-SPE) with further improved ionic conductivity promoted by constructing a dual range fast ionic conduction, which eventually shows a further improved ionic conductivity value of 2.3 × 10−5 S/cm at room temperature.
- Published
- 2022
41. Honeycomb-like carbon with doping of a transition-metal and nitrogen for highly efficient zinc–air battery and zinc-ion battery
- Author
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Pingyu Wan, Mingfei Shao, Qian Huang, Xiao Jin Yang, Ao Xie, Yu Chen, Shuxian Zhuang, Jinpeng Zhang, Xin You, Yongmei Chen, and Yang Tang
- Subjects
Battery (electricity) ,Materials science ,Renewable Energy, Sustainability and the Environment ,Energy Engineering and Power Technology ,chemistry.chemical_element ,Cathode ,Energy storage ,law.invention ,Anode ,Fuel Technology ,chemistry ,Chemical engineering ,Zinc–air battery ,law ,Carbon ,Voltage ,Power density - Abstract
The aqueous Zinc-base batteries such as the Zn-air battery and the Zn-ion battery, featured with low-cost, high safety, high specific capacity and environmental-friendliness, have attracted intensive attention for energy storage. For high performance Zinc-base batteries, the electrocatalysts with good conductivity, convenient mass transfer path and adequate accessible active sites are demanded. Herein, we have fabricated transition-metal/nitrogen co-doped honeycomb-like carbon materials with hierarchical porous structure through a freeze-drying and one-step pyrolysis method. The as-prepared FeZnN-C are used as cathode materials of Zinc-air battery, the Zinc-air battery displays high peak power density of 257 mW cm-2, superior discharging voltage platform around 1.36 V and large specific capacity of 785 mAh gZn-1. Meanwhile, the prepared Zn/ZnN-C was used as anode of a Zinc-ion battery which exhibits great cycle stability and high charging/discharge rate. Our work provides a novel and facile method to construct highly efficient electrocatalysts with hierarchical macro-meso-micro-porous structure and multi-active sites for application in the aqueous Zinc-base batteries.
- Published
- 2022
42. Lycopene ameliorates atrazine-induced pyroptosis in spleen by suppressing the Ox-mtDNA/Nlrp3 inflammasome pathway
- Author
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Xue-Nan Li, Shi-Yong Zhu, Jian-Ying Guo, Xue-Yan Dai, Jin-Yang Li, and Jin-Long Li
- Subjects
Inflammasomes ,Spleen ,medicine.disease_cause ,DNA, Mitochondrial ,chemistry.chemical_compound ,Lycopene ,NLR Family, Pyrin Domain-Containing 3 Protein ,Pyroptosis ,medicine ,Extracellular ,Humans ,Atrazine ,integumentary system ,Chemistry ,Inflammasome ,General Medicine ,Cell biology ,medicine.anatomical_structure ,IRF1 ,Oxidative stress ,Food Science ,medicine.drug - Abstract
Nlrp3 is a vital integration point of diverse extracellular stimuli and cellular stress. However, inappropriate activation of Nlrp3 results in progression of autoinflammatory and metabolic disorders. Atrazine, using widely in agricultural sector, is a toxic environmental factor for human. Here, this study found that atrazine could induce oxidative stress and the expression of Nfkb and IRF1 in spleen, promoting the ox-mtDNA formation. And production and release of ox-mtDNA stimulated the Nlrp3 inflammasome. Lastly, atrazine induced the pyroptosis in spleen mediating the activation of Nlrp3 inflammasome. Additionally, lycopene, a kind of carotenoid, is natural bioactive component in fruits and vegetables, which is applied towards reducing oxidative stress. It was found that lycopene could ameliorate the pyroptosis induced by atrazine via inhibition of the ox-mtDNA production. The results also provided evidence that lycopene had the potential role in prevention Nlrp3 inflammasome activation by depleting the ox-mtDNA.
- Published
- 2022
43. Layered Ag-graphene films synthesized by Gamma ray irradiation for stable lithium metal anodes in carbonate-based electrolytes
- Author
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Jiaxiang Liu, Nanbiao Pei, Huiyang Li, Peng Zhang, Jin Yang, Jinbao Zhao, Haoshen Ma, and Zhipeng Wen
- Subjects
Battery (electricity) ,Materials science ,Graphene ,Nucleation ,Energy Engineering and Power Technology ,chemistry.chemical_element ,02 engineering and technology ,Electrolyte ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Anode ,law.invention ,Fuel Technology ,Chemical engineering ,chemistry ,law ,Plating ,Electrochemistry ,Lithium ,0210 nano-technology ,Faraday efficiency ,Energy (miscellaneous) - Abstract
Lithium metal batteries are considered as high energy density battery systems with very promising prospects and have been widely studied. However, The uncontrollable plating/stripping behavior, infinite volume change and dendrites formation of lithium metal anode restrict the application. The uncontrolled nucleation of lithium caused by the nonuniform multi-physical field distributions, can lead to the undesirable lithium deposition. Herein, a graphene composite uniformly loaded with Ag nano-particles (Ag NPs) is prepared through a facile Gamma ray irradiation method and assembled into self-supported film with layered structure (Ag-rGO film). When such film is used as a lithium metal anode host, the uncontrolled deposition is converted into a highly nucleation-induced process. On one hand, the Ag NPs distributed between the interlayers of graphene can preferentially induce lithium nucleation and enable uniform deposition morphology of lithium between interlayers. On the other hand, the stable layered graphene structure can accommodate volume change, stabilize the interface between anode and electrolyte and inhibit dendrites formation. Therefore, the layered Ag-rGO film as anode host can reach a high Coulombic efficiency over 93.3% for 200 cycle (786 h) at a current density of 1 mA cm−2 for 2 mAh cm−2 in carbonate-based electrolyte. This work proposes a facile Gamma ray irradiation method to prepare metal/3D-skeleton structure as lithium anode host and demonstrates the potential to regulate the lithium metal deposition behaviors via manipulating the distribution of lithiophilic metal (e.g. Ag) in 3D frameworks. This may offer a practicable thinking for the subsequent design of the lithium metal anode.
- Published
- 2022
44. A Novel Method to Detect and Monitor Retinal Vasculitis Using Swept-Source OCT Angiography
- Author
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Janet L. Davis, Jin Yang, Yingying Shi, Thomas A. Albini, Giovanni Gregori, Philip J. Rosenfeld, and Jila Noori
- Subjects
Fovea Centralis ,medicine.medical_specialty ,Retinal thickening ,Fundus Oculi ,Population ,Visual Acuity ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Oct angiography ,Ophthalmology ,medicine ,Humans ,Fluorescein Angiography ,education ,Retrospective Studies ,030304 developmental biology ,Retinal Vasculitis ,0303 health sciences ,education.field_of_study ,Retina ,medicine.diagnostic_test ,Retinal vasculitis ,business.industry ,Retinal Vessels ,Retinal ,medicine.disease ,Fluorescein angiography ,medicine.anatomical_structure ,chemistry ,030221 ophthalmology & optometry ,Vasculitis ,business ,Tomography, Optical Coherence - Abstract
Purpose To introduce a novel method for assessment of retinal vasculitis using swept-source OCT angiography (SS-OCTA). Design Retrospective case series. Participants Patients with retinal vasculitis. Methods The subjects were identified among the clinic population and imaged with 12 × 12-mm SS-OCTA scans centered on the fovea. A custom retina segmentation superimposed the color retinal thickness map on a modified en face flow scan. Findings from en face flow scans were correlated with localized perivascular retinal thickening on B-scans. Results from SS-OCTA were compared with fluorescein angiography (FA) to examine the proportion of perivascular thickening to retinal vascular leakage or staining. Results Twenty-one patients with retinal vasculitis underwent same-day FA and SS-OCTA. Visible retinal vascular leakage/staining on FA corresponded to increased perivascular retinal thickness on SS-OCTA in 17 patients. Five patients had a second examination with same-day FA and SS-OCTA after treatment of the vasculitis. Three of those 5 patients showed improved retinal vascular leakage/staining on post-treatment FA and decreased perivascular retinal thickness on SS-OCTA scans. Conclusions Swept-source OCT angiography detects structural retinal thickening secondary to inflammatory retinal vascular leakage. Further studies are required to confirm whether SS-OCTA may serve as a semiquantitative alternative to FA to diagnose and monitor the response to treatment in patients with retinal vasculitis.
- Published
- 2021
45. Simultaneous Determination of 6 Antiretroviral Drugs in Human Hair Using an LC-ESI+-MS/MS Method: Application to Adherence Assessment
- Author
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Shan Qiao, Zhiyong Shen, Liuxi Chu, Shuaifeng Liu, Haoran Yang, Huihua Deng, Xiaoming Li, Yuejiao Zhou, Wei Wang, Yan Wu, Quan Zhang, and Jin Yang
- Subjects
Efavirenz ,Nevirapine ,HIV Infections ,Pharmacology ,Tandem mass spectrometry ,Article ,chemistry.chemical_compound ,Zidovudine ,Tandem Mass Spectrometry ,Humans ,Medicine ,Pharmacology (medical) ,Chromatography, High Pressure Liquid ,business.industry ,Selected reaction monitoring ,Reproducibility of Results ,Lamivudine ,Lopinavir ,Pharmaceutical Preparations ,chemistry ,Ritonavir ,business ,Chromatography, Liquid ,Hair ,medicine.drug - Abstract
BACKGROUND: The determination of antiretroviral drugs in hair is receiving considerable research interest to assess long-term adherence to antiretroviral therapy (ART). Currently in China, lamivudine, zidovudine, nevirapine, efavirenz, ritonavir, and lopinavir are combined as first- and second-line free therapy regimens and are recommended for people living with human immunodeficiency virus (HIV) (PLWH). Simultaneous determination of the six antiretroviral drugs in human hair is important for accurately and widely assessing long-term adherence in Chinese PLWH receiving different ART regimens. METHODS: Six drugs were extracted from 10-mg hair samples incubated in methanol for 16 h at 37 °C and then analyzed by liquid chromatography with tandem mass spectrometry (LC-MS/MS) using a mobile phase of 95% methanol, with an electrospray ionization (ESI) source in multiple reaction monitoring and positive mode. RESULTS: The LC-ESI(+)-MS/MS method exhibited a linear range (R(2) > 0.99) within 6–5000, 10–5000, 6–50000, 12–50000, 8–5000, and 8–12500 pg/mg for lamivudine, zidovudine, nevirapine, efavirenz, ritonavir, and lopinavir. For all six drugs, the limits of quantification ranged between 6 and 12 pg/mg. The intra-day and inter-day coefficients of variation were within 15%, and the recoveries ranged from 91.1% to 113.7%. Furthermore, the other validation parameters (i.e., selectivity, matrix effect, stability, and carryover) met the acceptance criteria stipulated by guidelines of the United States Food and Drug Administration and European Medicines Agency. Significant intergroup differences were observed between high- and low-adherence groups, with high inter-correlations in the hair content of the six drugs. CONCLUSIONS: The developed method demonstrated good reliability, to comprehensively and accurately assess adherence in PLWH receiving different ART regimens.
- Published
- 2021
46. Predicting Model of Sound Absorbing Properties of Cellulose-based Porous Materials Prepared by Foam Forming
- Author
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Jin Yang, Xiwen Wang, and Kaihui Dong
- Subjects
chemistry.chemical_compound ,geography ,geography.geographical_feature_category ,Materials science ,chemistry ,Materials Science (miscellaneous) ,Composite material ,Cellulose ,Porous medium ,Sound (geography) - Published
- 2021
47. Relaxing Effect of Novel Cosmetic Ingredient using Lactobacillus gasseri HDB1102 on Skin Problems Caused by Particulate Matter
- Author
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Seo-Jin Yang, Kyung-Min Kim, Ji-Won Song, and Seung-Hun Lee
- Subjects
Cosmetic ingredient ,biology ,Chemistry ,Food science ,Particulates ,Lactobacillus gasseri ,biology.organism_classification - Abstract
In this study, we developed Dermabiotics HDB1102 using Lactobacillus gasseri HDB1102 to relieve skin irritation caused by particulate matter (PM). L. gasseri HDB1102 was provided from cell bank and identified by 16S ribosomal RNA gene sequencing. Dermabiotics HDB1102 was manufactured by heating, centrifuging, and filtering culture medium of L. gasseri HDB1102. When 0-2.5%(v/v) Dermabiotics HDB1102 was treated, cytotoxicity on normal human epidermal keratinocytes (NHEKs) and human fibroblast was not observed by using MTT assay. The mRNA expression levels of cytochrome P450 1A1 (CYP1A1), interleukin (IL)-1β, and IL-8 on Dermabiotics HDB1102 treated cells decreased compared to PM-treated cells. Conversely, the mRNA expressions of aquaporin-3 (AQP-3), CD-44, and collagen type 1 (COL-1) on Dermabiotics HDB1102 treated cells were dose-dependent higher than those of non-treated cells. These results indicated that Dermabiotics HDB1102 have anti-inflammatory, moisturizing, and anti-wrinkle effects and could be used as a potential cosmetic ingredient to alleviate skin symptoms caused by PM.
- Published
- 2021
48. Fabrication of carbon dots for sequential on–off-on determination of Fe3+ and S2− in solid-phase sensing and anti-counterfeit printing
- Author
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Jin Yang, Zheng Yang, Huaqi Zhao, Haiyan Bai, Mengyao She, Yulong Li, Yiting Ma, Xilang Jin, and Hongwei Zhou
- Subjects
Detection limit ,Fabrication ,Materials science ,Filter paper ,Quantum yield ,chemistry.chemical_element ,Nanotechnology ,Biochemistry ,Fluorescence ,Analytical Chemistry ,chemistry ,Phase (matter) ,Biological imaging ,Carbon - Abstract
Glutathione and 2-aminopyridine are used as carbon sources to prepare carbon dots (CDs) by a one-step hydrothermal reaction. The results show that the average particle diameter of CDs is 8.64 nm with uniform size distribution and the fluorescence quantum yield is 13.62%. We further demonstrate that novel CDs possess highly selective sensing of Fe3+ from 0.2 to 200 μM with a low detection limit (0.194 μM). Meanwhile, the fluorescence of CDs can be repeated many times by the addition of S2−. Moreover, the CDs are used for biological imaging of living cells with well cell penetrability and low toxicity. Furthermore, it is successfully applied for anti-counterfeiting and information encryption. More interestingly, it can be doped with hydrogel and filter paper to prepare solid-phase sensors exhibiting high sensitivity and fast response, demonstrating their tremendous potential for the simple, rapid, and low-cost monitoring of Fe3+ and S2−.
- Published
- 2021
49. Construction of nanofibrous scaffolds with interconnected perfusable microchannel networks for engineering of vascularized bone tissue
- Author
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Jin Yang, Haibo Du, Jiani Gu, Mengru Geng, Weizhong Wang, Atta ur Rehman Khan, Chuanglong He, Zhou Sha, Qianqian Zhang, and Xiaojun Zhou
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Scaffold ,Materials science ,Angiogenesis ,QH301-705.5 ,0206 medical engineering ,Biomedical Engineering ,02 engineering and technology ,Umbilical vein ,Article ,Biomaterials ,chemistry.chemical_compound ,Biology (General) ,Bone regeneration ,Materials of engineering and construction. Mechanics of materials ,Microchannel ,Vascularization ,3D printing ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,Vascular endothelial growth factor ,Chorioallantoic membrane ,Microchannel networks ,chemistry ,Microangiography ,TA401-492 ,0210 nano-technology ,Biotechnology ,Biomedical engineering - Abstract
Vascularization and bone regeneration are two closely related processes during bone reconstruction. A three-dimensional (3D) scaffold with porous architecture provides a suitable microenvironment for vascular growth and bone formation. Here, we present a simple and general strategy to construct a nanofibrous poly(l-lactide)/poly(ε-caprolactone) (PLLA/PCL) scaffold with interconnected perfusable microchannel networks (IPMs) based on 3D printing technology by combining the phase separation and sacrificial template methods. The regular and customizable microchannel patterns within the scaffolds (spacings: 0.4 mm, 0.5 mm, and 0.6 mm; diameters: 0.8 mm, 1 mm, and 1.2 mm) were made to investigate the effect of microchannel structure on angiogenesis and osteogenesis. The results of subcutaneous embedding experiment showed that 0.5/0.8-IPMs (spacing/diameter = 0.5/0.8) and 0.5/1-IPMs (spacing/diameter = 0.5/1) scaffolds exhibited more vascular network formation as compared with other counterparts. After loading with vascular endothelial growth factor (VEGF), VEGF@IPMs-0.5/0.8 scaffold prompted better human umbilical vein endothelial cells (HUVECs) migration and neo-blood vessel formation, as determined by Transwell migration, scratch wound healing, and chorioallantoic membrane (CAM) assays. Furthermore, the microangiography and rat cranial bone defects experiments demonstrated that VEGF@IPMs-0.5/0.8 scaffold exhibited better performance in vascular network formation and new bone formation compared to VEGF@IPMs-0.5/1 scaffold. In summary, our results suggested that the microchannel structure within the scaffolds could be tailored by an adjustable caramel-based template strategy, and the combination of interconnected perfusion microchannel networks and angiogenic factors could significantly enhance vascularization and bone regeneration., Graphical abstract Schematic diagram of nanofibrous scaffolds with interconnected perfusable microchannel networks for engineering of vascularized bone tissue.Image 1, Highlights • 3D-printed sacrificial templates are used to construct the scaffold with interconnected perfusable microchannel networks. • The microchannel structure within scaffolds can be tailored by changing the template specifications. • The introduction of VEGF in the microchannel of scaffold promotes the vascular network formation. • Microchannel structure and angiogenic factor within scaffold significantly enhance vascularization and bone regeneration.
- Published
- 2021
50. Expression profiles of tyrosine metabolic pathway genes and functional analysis of <scp>DOPA</scp> decarboxylase in puparium tanning of Bactrocera dorsalis ( <scp>Hendel</scp> )
- Author
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Jin-Jun Wang, Er-Hu Chen, Qiu-Li Hou, Wei Dou, and Pei-Jin Yang
- Subjects
chemistry.chemical_classification ,Aromatic L-amino acid decarboxylase ,biology ,Tephritidae ,fungi ,General Medicine ,biology.organism_classification ,Bactrocera dorsalis ,Enzyme assay ,Dihydroxyphenylalanine ,Metabolic pathway ,Enzyme ,Biochemistry ,chemistry ,Insect Science ,Dopa Decarboxylase ,biology.protein ,Animals ,Insect Proteins ,Tyrosine ,Cuticle pigmentation ,Agronomy and Crop Science ,Metabolic Networks and Pathways ,Pupariation - Abstract
Background Tanning is an important physiological process that play the critical roles in affecting cuticle pigmentation and sclerotization. Previous studies have showed that insect cuticle tanning was closely associated with tyrosine metabolism pathway that consisted of a series of enzymes. Results In this study, a total of 24 tyrosine metabolism pathway genes were identified in the oriental fruit fly Bactrocera dorsalis (Hendel) genome. Then, the gene expression profiles throughout 15 developmental stages of B. dorsalis were established on the basis of our previous RNA-seq data, and we found 13 enzyme genes could be involved in the process of pupariation. Accordingly, a predicted tyrosine-mediated tanning pathway during the pupariation of B. dorsalis was obtained, and a critical enzyme 3,4-dihydroxyphenylalanine (DOPA) decarboxylase (DDC) was selected to explore its possible functions in the formation of puparium. Firstly, the RT-qPCR confirmed that BdDDC had epidermis-specific expression pattern, and it was also highly expressed during the larval metamorphosis of B. dorsalis. Subsequently, the dysfunction of BdDDC by feeding 5-day-old larvae with DDC inhibitor (L-α-Methyl-DOPA) could lead to (i) the significant decreasing of BdDDC enzyme activity and dopamine concentration; (ii) the defects in puparium pigmentation; (iii) the impairment of morphology and less thickness of puparium; (iv) the lower pupal weight and obstacle to eclosion. Conclusion The present study provided us a potential tyrosine metabolic pathway that was responsible for insect tanning during the pupariation, and the BdDDC enzyme has been proved to act the crucial roles in larval-pupal tanning of B. dorsalis. This article is protected by copyright. All rights reserved.
- Published
- 2021
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