1. A guanylurea-functionalized conjugated polymer enables RNA interference in ex vivo human airway epithelium
- Author
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Ping Wang, Md. Salauddin Ahmed, Rajib Kumar Dutta, Xuerong Li, Prakash Manandhar, Hooman Torabi, Srinivasan Chinnapaiyan, Alfonso Barrios, Hoshang J. Unwalla, and Joong Ho Moon
- Subjects
Polymers ,Respiratory Mucosa ,Conjugated system ,010402 general chemistry ,Guanidines ,01 natural sciences ,Histone Deacetylases ,Catalysis ,RNA interference ,Materials Chemistry ,medicine ,Humans ,Urea ,RNA, Small Interfering ,Gene ,Cells, Cultured ,Drug Carriers ,Gene knockdown ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Metals and Alloys ,Epithelial Cells ,General Chemistry ,respiratory system ,Mucus ,Epithelium ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Cell biology ,medicine.anatomical_structure ,Gene Knockdown Techniques ,Ceramics and Composites ,Surface modification ,RNA Interference ,Ex vivo - Abstract
We demonstrate a successful target gene knockdown in ex vivo normal human bronchial epithelium (NHBE) cells covered with mucus layers using the guanylurea functionalization technique modulating the chemical environment at the positive charge of a gene carrier.
- Published
- 2019