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In order to effectively control petroleum hydrocarbon pollution by immobilisation technology, it is necessary to understanding the degradation pathways of petroleum hydrocarbon in immobilised petroleum-degradation bacteria system. However, the adsorption in degradation process is rarely studied. In this study, adsorption process of petroleum hydrocarbon was synergistically studied by means of the surface properties, adsorption thermodynamics and molecular simulation. The results indicate that the immobilised petroleum-degradation bacteria have many holes for the bacteria to adsorb. The diesel adsorption by immobilised petroleum-degradation bacteria is a spontaneous, entropy-increasing and endothermic process. Diesel is first adsorbed to the surface of immobilised petroleum-degradation bacteria through hydrogen bonding, and then is biodegraded. This study provided substantial knowledge of immobilised technology in controlling petroleum hydrocarbon pollution.

The nucleosome remodeling factor (NURF) alters chromatin accessibility through interactions with its largest subunit,the bromodomain PHD finger transcription factor BPTF. BPTF is overexpressed in several cancers and is an emerging anticancer target. Targeting the BPTF bromodomain presents a potential strategy for its inhibition and the evaluation of its functional significance; however, inhibitor development for BPTF has lagged behind those of other bromodomains. Here we describe the development of pyridazinone-based BPTF inhibitors. The lead compound, BZ1, possesses a high potency (Kd = 6.3 nM) and >350-fold selectivity over BET bromodomains. We identify an acidic triad in the binding pocket to guide future designs. We show that our inhibitors sensitize 4T1 breast cancer cells to doxorubicin but not BPTF knockdown cells, suggesting a specificity to BPTF. Given the high potency and good physicochemical properties of these inhibitors, we anticipate that they will be useful starting points for chemical tool development to explore the biological roles of BPTF.

Phase change materials are functional materials that have the potential to cool asphalt pavement. This study focuses on the design of a stearic acid/palmitic acid binary eutectic phase change material (SA/PA-PCM) and its utilization in asphalt binder by conducting a series of tests. The results show that the dislocation density, micro-strain, and crystallinity of SA/PA-PCM increase by approximately 432%, 140%, 6%, respectively, which means that it can have a more stable crystal structure. Therefore, the SA/PA-PCM has a higher phase transition enthalpy (226.9 J/g) and a better phase transition temperature (54.8 °C) than traditional materials. Moreover, no chemical reaction is detected in the eutectic and modified asphalt binder processes. Crystalline SA/PA-PCM has the potential to increase the anti-deforming capability of the binder, whereas liquid SA/PA-PCM suppresses the rheological behaviors. The decomposition temperature of SA/PA-PCM (approximately 200 °C) is higher than that of two kinds of individual fatty acids. Meanwhile, it is still above the traditional hot-mix asphalt pavement construction temperatures. Thus, we concluded that SA/PA-PCM has exceptional phase change characteristics and is applicable for use in cooling asphalt binder. Nevertheless, encapsulating technologies must be adopted to prevent the deleterious impact of the liquefiable phase change materials on the binder.

Although a plethora of gene carriers have been developed for potential gene therapy, imageable stimuli-responsive gene vectors with fast access to the nucleus, high biocompatibility, and transfecti...

Kinesin-like calmodulin-binding protein (KCBP) is a unique kinesin with half kinesin and half myosin, with kinesin motor domain at C-terminus and myosin tail homology region 4 (MyTH4) and band 4.1, ezrin, radixin, moesin (FERM) domains at N-terminus. The special structure endows KCBP multi-intracellular functions, including cell division, trichome morphogenesis in plants, and flagellar function in algae. However, little is known about the molecular mechanism underlying these functions. Here, we identified a molecular chaperone Hsp90 as a novel binding partner with KCBP in Dunaliella salina using a yeast two-hybrid screen. Further analysis showed that Hsp90 interacted with both the N-terminal and C-terminal of DsKCBP. Since Hsp90 was involved in the stability and proteolytic turnover of numerous proteins, whether Hsp90 regulated the degradation of DsKCBP was investigated. Our results showed that both Hsp90 and DsKCBP presented in the purified proteasome, and the interaction of DsKCBP-Hsp90 was inhibited upon Hsp90 inhibitor geldanamycin treatment. The level of DsKCBP proteins was diminished remarkably indicating that the disassociation of DsKCBP from Hsp90 accelerated the degradation of the former. Furthermore, immunofluorescence results showed that the localization of DsKCBP at basal body and flagella was disappeared by Hsp90 inhibition. The increased mRNA level of DsKCBP during flagellar assembly was not obvious by geldanamycin treatment. These data provided evidence that Hsp90 protected DsKCBP from degradation by proteasome and was involved in the role of DsKCBP in flagellar assembly.

Soluble methane monooxygenase (sMMO) is a multicomponent metalloenzyme capable of catalyzing the fissure of the C-H bond of methane and the insertion of one atom of oxygen from O2 to yield methanol. Efficient multiple-turnover catalysis occurs only in the presence of all three sMMO protein components: hydroxylase (MMOH), reductase (MMOR), and regulatory protein (MMOB). The complex series of sMMO protein component interactions that regulate the formation and decay of sMMO reaction cycle intermediates is not fully understood. Here, the two tryptophan residues in MMOB and the single tryptophan residue in MMOR are converted to 5-fluorotryptophan (5FW) by expression in defined media containing 5-fluoroindole. In addition, the mechanistically significant N-terminal region of MMOB is 19F-labeled by reaction of the K15C variant with 3-bromo-1,1,1-trifluoroacetone (BTFA). The 5FW and BTFA modifications cause minimal structural perturbation, allowing detailed studies of the interactions with sMMOH using 19F NMR. Resonances from the 275 kDa complexes of sMMOH with 5FW-MMOB and BTFA-K15C-5FW-MMOB are readily detected at 5 μM labeled protein concentration. This approach shows directly that MMOR and MMOB competitively bind to sMMOH with similar KD values, independent of the oxidation state of the sMMOH diiron cluster. These findings suggest a new model for regulation in which the dynamic equilibration of MMOR and MMOB with sMMOH allows a transient formation of key reactive complexes that irreversibly pull the reaction cycle forward. The slow kinetics of exchange of the sMMOH:MMOB complex is proposed to prevent MMOR-mediated reductive quenching of the high-valent reaction cycle intermediate Q before it can react with methane.

By referring to the successful application of a cold-resistant plasticizer in the plastic industry, dioctyl adipate (DOA) plasticizer was selected to improve the low-temperature performance of asph...

Replication initiator proteins (Reps) from the HUH-endonuclease superfamily process specific single-stranded DNA (ssDNA) sequences to initiate rolling circle/hairpin replication in viruses, such as crop ravaging geminiviruses and human disease causing parvoviruses. In biotechnology contexts, Reps are the basis for HUH-tag bioconjugation and a critical adeno-associated virus genome integration tool. We solved the first co-crystal structures of Reps complexed to ssDNA, revealing a key motif for conferring sequence specificity and for anchoring a bent DNA architecture. In combination, we developed a deep sequencing cleavage assay, termed HUH-seq, to interrogate subtleties in Rep specificity and demonstrate how differences can be exploited for multiplexed HUH-tagging. Together, our insights allowed engineering of only four amino acids in a Rep chimera to predictably alter sequence specificity. These results have important implications for modulating viral infections, developing Rep-based genomic integration tools, and enabling massively parallel HUH-tag barcoding and bioconjugation applications.

The challenge for fully exerting the excellent nature of graphene oxide (GO) within polymer composites was to realize its uniform dispersion and strong interfacial bonding in the polymer matrix thr...

Dendrimers are well-defined, highly branched macromolecules that have been widely applied in the fields of catalysis, sensing, and biomedicine. Here, we present a novel multifunctional photochromic dendrimer fabricated through grafting azobenzene units onto dendrimers, which not only enables controlled switching of adhesives and effective repair of coating scratches but also realizes high-performance solar energy storage and on-demand heat release. The switchable adhesives and healable coatings of azobenzene-containing dendrimers are attributed to the reversible solid-to-liquid transitions because trans-isomers and cis-isomers have different glass transition temperatures. The adhesion strengths increase significantly with the increase in dendrimer generations, wherein the adhesion strength of fifth-generation photochromic dendrimers (G5-Azo) can reach up to 1.62 MPa, five times higher than that of pristine azobenzenes. The solar energy storage and heat release of dendrimer solar thermal fuels, the isomers of which possess different chemical energies, can be also enhanced remarkably with the amplification of azobenzene groups on dendrimers. The storage energy density of G5-Azo can reach 59 W h kg-1, which is much higher than that of pristine azobenzenes (36 W h kg-1). The G5-Azo fuels exhibit a 5.2 °C temperature difference between cis-isomers and trans-isomers. These findings provide a new perspective and tremendously attractive avenue for the fabrication of photoswitchable adhesives and coatings and solar thermal fuels with dendrimer structures.

Catalyst-free synthesis in three kinds of isocyanate-blocked imidazole compound was successfully achieved by chemically connecting 2-ethyl-4-methylimidazole (2E4MI) with hexamethylene diisocyanate (HDI), trimethylhexamethylene diisocyanate (TMHDI) or 4,4′-diphenylmethane diisocyanate (MDI), which were tested as the latent hardeners for epoxy (EP) resins. The chemical structures of the synthesized HDI-2E4MI, TMHDI-2E4MI and MDI-2E4MI were identified with Fourier transform infrared spectroscope (FTIR), 1H and 13C nuclear magnetic resonance (1H NMR and 13C NMR). Based on characterizations including thermogravimetry/differential scanning calorimetry (TG/DSC) simultaneous testing and hot-stage FTIR, it was found that both HDI-2E4MI and TMHDI-2E4MI exhibited two-step deblocking behavior, whereas the deblocking process of MDI-2E4MI was completed only via one step. Curing kinetics of HDI-2E4MI/EP, TMHDI-2E4MI/EP and MDI-2E4MI/EP systems were then studied by non-isothermal DSC using Kissinger model. The results revealed that MDI-2E4MI/EP system could be cured via a two-step reaction with the highest curing rate due to the high reactivity of MDI-2E4MI, which was much different from the four-step curing behaviors of both HDI-2E4MI/EP and TMHDI-2E4MI/EP systems. Compared to the reference 2E4MI, noticeably, the three synthesized hardeners showed the outstanding latent features with lower curing activity and longer shelf life and the EP systems cured by the hardeners exhibited desirable mechanical properties and thermal stability. This study provided a feasible approach to synthesize high-performance imidazole-based latent hardener for wide practical applications in a greenway via the blocking treatment through tailoring the isocyanate structure.

The crystal structure of a bacteriophage T4 early gene product, Spackle, was determined by native sulfur single-wavelength anomalous diffraction (SAD) phasing using synchrotron radiation and was refined to 1.52 Å resolution. The structure shows that Spackle consists of a bundle of five α-helices, forming a relatively flat disc-like overall shape. Although Spackle forms a dimer in the crystal, size-exclusion chromatography with multi-angle light scattering shows that it is monomeric in solution. Mass spectrometry confirms that purified mature Spackle lacks the amino-terminal signal peptide and contains an intramolecular disulfide bond, consistent with its proposed role in the periplasm of T4 phage-infected Escherichia coli cells. The surface electrostatic potential of Spackle shows a strikingly bipolar charge distribution, suggesting a possible mode of membrane association and inhibition of the tail lysozyme activity in T4 bacteriophage superinfection exclusion.

The metalloenzyme soluble methane monooxygenase (sMMO) consists of hydroxylase (sMMOH), regulatory (MMOB), and reductase components. When sMMOH forms a complex with MMOB, the rate constants are greatly increased for the sequential access of O2, protons, and CH4 to an oxygen-bridged diferrous metal cluster located in the buried active site. Here, we report high-resolution X-ray crystal structures of the diferric and diferrous states of both sMMOH and the sMMOH:MMOB complex using the components from Methylosinus trichosporium OB3b. These structures are analyzed for O2 access routes enhanced when the complex forms. Previously reported, lower-resolution structures of the sMMOH:MMOB complex from the sMMO of Methylococcus capsulatus Bath revealed a series of cavities through sMMOH postulated to serve as the O2 conduit. This potential role is evaluated in greater detail using the current structures. Additionally, a search for other potential O2 conduits in the M. trichosporium OB3b sMMOH:MMOB complex revealed a narrow molecular tunnel, termed the W308-tunnel. This tunnel is sized appropriately for O2 and traverses the sMMOH-MMOB interface before accessing the active site. The kinetics of reaction of O2 with the diferrous sMMOH:MMOB complex in solution show that use of the MMOB V41R variant decreases the rate constant for O2 binding >25000-fold without altering the component affinity. The location of Val41 near the entrance to the W308-tunnel is consistent with the tunnel serving as the primary route for the transfer of O2 into the active site. Accordingly, the crystal structures show that formation of the diferrous sMMOH:MMOB complex restricts access through the chain of cavities while opening the W308-tunnel.

Biodegradation characteristics of polycaprolactone (PCL) films with three different molecular weights after being degraded by Candida antarctica lipase were investigated. The changes of the PCL films before and after degradation were characterized. The results showed that the degradation process of PCL films could be roughly divided into two phrases: a rapid degradation phase and a slow degradation phase. Molecular weights of PCL films had no influence on the performance of lipase; however, increasing molecular weight caused the numbers of pores on the degraded PCL films to increase, and the pores became deeper as the degradation time increased. The results also showed that the crystallinity of the films decreased with increasing degradation time, and both the crystalline and the amorphous regions of the films were simultaneously degraded. The thermal stability of the films also gradually decreased with the increase of degradation time. Despite the above changes, the degradation did not cause the chemical composition and structure of PCL to change, and PCL remained the main component.

Poly(butylene succinate) (PBS) and poly(lactic acid) (PLA) were melt-blended and formed into a film by hot press forming. The film was selectively degraded by cutinase and proteinase K to form a porous material. The porous materials were characterized with respect to their pore morphology, pore size, porosity and hydrophilicity. The porous materials were investigated in vitro degradation and in vivo compatibility. The results show that the pore size of the prepared porous materials could be controlled by the proportion of PBS and the degradation time. When the PBS composition of PBS/PLA blends was changed from 40 wt% to 50 wt%, the mean pore diameter of the porous materials significantly increased from 6.91 µm to 120 µm, the porosity improved from 81.52% to 96.90%, and the contact angle decreased from 81.08° to 46.56°. In vitro degradation suggests that the PBS-based porous materials have a good corrosion resistance but the PLA-based porous materials have degradability in simulated body fluid. Subcutaneous implantation of the porous materials did not cause intense inflammatory response, which revealed good compatibility. The results of hematoxylin and eosin and Masson's trichrome staining assays demonstrated that the porous materials promote chondrocyte production. Porous materials have great potential in preparing implants for tissue engineering applications.

Aim To observe the effect of inhibiting long non-coding RNA (lncRNA) metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) on diabetic neurodegeneration. Methods Thirty-six 8-week-old C57BL/6 mice were randomly divided into normal control, diabetic control, diabetic scrambled small interfering RNAs (siRNAs) and diabetic MALAT1-siRNA groups. After diabetic induction with streptozocin intraperitoneally-injection, the diabetic MALAT1-siRNA group was intravitreally injected with 1 µL 20 µmol/L MALAT1 siRNA, and the diabetic scrambled siRNA group was injected with the same amount of scrambled siRNA. Electroretinography was performed to examine photoreceptor functions 16wk after diabetes induction. MALAT1 expression was detected via real time polymerase chain reaction. Cone morphological changes were examined using immunofluorescence. Rod morphological changes were examined by determining outer nuclear layer (ONL) thickness. Results The upregulation of retinal MALAT1 expression was detected in the diabetic control mice, while MALAT1 expression in the diabetic MALAT1-siRNA mice was decreased by 91.48% compared to diabetic control mice. The diabetic MALAT1-siRNA and diabetic control mice showed lower a-wave and b-wave amplitudes than did the normal control mice in scotopic and photopic electroretinogram, while the diabetic MALAT1-siRNA mice showed higher amplitudes than diabetic control mice. Morphological examination revealed that ONL thickness in the diabetic MALAT1-siRNA and diabetic control mice was lower than normal control mice. However, ONL thickness was greater in the diabetic MALAT1-siRNA mice than diabetic control mice. Moreover, the diabetic control mice performed a sparser cone cell arrangement and shorter outer segment morphology than diabetic MALAT1-siRNA mice. Conclusion Inhibiting retinal MALAT1 results in mitigative effects on the retinal photoreceptors, thus alleviating diabetic neurodegeneration.

Poly(ε-caprolactone) (PCL) particles were melt-pressed into films using a hot press and then subjected to degradation by lipase from Candida antarctica and cutinase from Fusarium solani, respectively. The differences in weight loss, degradation modes, thermal stability, and crystallization were investigated after degradation by two kinds of enzymes. The result showed that mass loss of PCL films degraded by lipase was higher than that degraded by cutinase at the same enzyme concentrations. The degradation mode of PCL films is layered for cutinase degradation and penetrated for lipase degradation. Crystallinity of PCL had no obvious decrease after degradation by cutinase, but it markedly decreased after lipase-degradation. PCL films occurred one-step decomposition during heating and the cutinase-degraded products had similar thermal stability. Whereas the thermal stability of lipase-degraded PCL decreased significantly and the weight loss of the PCL occurred in several steps with increasing lipase hydrolysis time.

Fowlpox virus resolvase (Fpr) is an endonuclease that cleaves a broad range of branched DNA structures, including the Holliday junction (HJ), with little sequence-specificity. To better understand the mechanisms underlying its relaxed substrate specificity, we determined the crystal structures of Fpr and that in a novel complex with HJ at 3.1-Å resolution. In the Fpr-HJ complex, two Fpr dimers use several distinct regions to interact with different DNA structural motifs, showing versatility in DNA-binding. Biochemical and solution NMR data support the existence of non-canonical modes of HJ interaction in solution. The binding of Fpr to various DNA motifs are mediated by its flat DNA-binding surface, which is centered on a short loop spanning K61 to I72 and flanked by longer α-helices at the outer edges, and basic side grooves near the dimer interface. Replacing the Fpr loop K61~I72 with a longer loop from Thermus thermophilus RuvC (E71~A87) endows Fpr with an enhanced selectivity toward HJ cleavage but with a target sequence preference distinct from that of RuvC, highlighting a unique role of this loop region in Fpr-HJ interaction. Our work helps explain the broad substrate selectivity of Fpr and suggests a possible mode of its association with poxvirus hairpin telomeres.

High-fidelity replication of the large RNA genome of coronaviruses (CoVs) is mediated by a 3'-to-5' exoribonuclease (ExoN) in nonstructural protein 14 (nsp14), which excises nucleotides including antiviral drugs misincorporated by the low-fidelity viral RNA-dependent RNA polymerase (RdRp) and has also been implicated in viral RNA recombination and resistance to innate immunity. Here, we determined a 1.6-Å resolution crystal structure of severe acute respiratory syndrome CoV 2 (SARS-CoV-2) ExoN in complex with its essential cofactor, nsp10. The structure shows a highly basic and concave surface flanking the active site, comprising several Lys residues of nsp14 and the N-terminal amino group of nsp10. Modeling suggests that this basic patch binds to the template strand of double-stranded RNA substrates to position the 3' end of the nascent strand in the ExoN active site, which is corroborated by mutational and computational analyses. We also show that the ExoN activity can rescue a stalled RNA primer poisoned with sofosbuvir and allow RdRp to continue its extension in the presence of the chain-terminating drug, biochemically recapitulating proofreading in SARS-CoV-2 replication. Molecular dynamics simulations further show remarkable flexibility of multidomain nsp14 and suggest that nsp10 stabilizes ExoN for substrate RNA binding to support its exonuclease activity. Our high-resolution structure of the SARS-CoV-2 ExoN-nsp10 complex serves as a platform for future development of anticoronaviral drugs or strategies to attenuate the viral virulence.

Background. Retinal vein occlusion (RVO) is the second most common vision-threatening retinal vascular disease. Intravitreal dexamethasone implant has been applied to treat macular edema secondary to RVO (RVO-ME). However, the alteration of morphologic features detected with optical coherence tomography angiography (OCTA) has not been fully studied in RVO-ME patients before and after the treatment. Objective. This study is aimed at identifying potential therapeutic targets in RVO with integrative bioinformatic analysis and compares the OCTA characteristics alterations in patients with RVO-ME receiving injection of dexamethasone intravitreal implant. Methods. Bioinformatic analysis was analyzed in GSE101398 dataset from the Gene Expression Omnibus database. Multiple functional enrichment analyses were performed, and protein-protein interaction network was constructed to visualize the key node genes. Eleven eyes with RVO-ME were examined with OCTA before and after intravitreal dexamethasone implant. The OCTA parameters, including macular thickness, vessel density, foveal avascular zone parameters, the number of hyperreflective foci (HRF), area of cystoid edema, and subretinal fluid (SRF), were compared. The correlation was analyzed between best-corrected visual acuity (BCVA) and OCTA parameters. Results. A total of 79 differentially expressed genes were identified. Functional enrichment analyses revealed the enriched inflammatory events in RVO. In RVO-ME, Pearson correlation revealed that baseline BCVA was positively correlated with the area of SRF and central macular thickness, while no correlation was detected between baseline BCVA and HRF number or the area of cystoid edema. The visual acuity improved, and the central macular thickness was decreased after intravitreal dexamethasone implant injection. Besides, the number of HRF, the area of cystoid edema, and SRF were significantly alleviated after dexamethasone intravitreal injection. Conclusion. Retinal inflammation plays a crucial role in RVO pathogenesis. The imaging biomarkers of RVO including Müller glial intracellular edema, and retinal pigment epithelium dysfunction, could be assessed in OCTA and attenuated by intravitreal dexamethasone implant effectively.

The corrosion characteristics of 12Cr2W2Mn Ferritic/martensitic (F/M) steel before and after Au-ions irradiation in static lead–bismuth eutectic (LBE) have been investigated at 400 °C for 100 h. The results show that the samples without irradiation have many unequal size Fe3O4 particles and little Cr-rich oxide after LBE corrosion test, and the thickness of corrosion layer is 4.4 μm. The irradiation samples display plenty compact triangular (Fe,Cr)3O4 and some Cr-rich oxide, and the thickness of corrosion layer is 4.4 μm with some cracks. Au-ions irradiation enahnced the LBE corrosion of F/M steel, which could be attributed to the irradiation defects provide diffusion path of O atoms in liquid LBE. A schematic model was presented to understand the effect of irradiation damage on LBE corrosion behavior of F/M steel.

The plastic flow behaviors of FCC polycrystalline aluminum after pre-cyclic tension-compression deformation are mainly investigated in tension–torsion stress space by the physically based crystal plasticity model introducing a back-stress. A global finite element model (GFEM) constructed of sufficient grains was established to simulate the same-size thin-walled tube specimen constrained and loaded as the experiments of yield surfaces. The computational results showed that the shape of subsequent yield surfaces and the plastic flow directions directly depended on the given offset strain levels and the applied re-loading paths under different pre-cyclic deformations. The angle deviation between the plastic flow direction and the theoretical orthogonal direction further indicated that there was a large difference between them in the inverse pre-straining direction, but the difference was negligible in the pre-straining direction. From the influence of the anisotropic evolution of the subsequent yield surfaces on plastic flow, we found that the plastic normality rule followed the smooth yield locus, conversely, the significant non-associated flow was attributed to the distorted yield locus. Furthermore, it was also demonstrated that the anisotropic evolution and the plastic flow trend of the subsequent yield surfaces obtained by experiments can be better reproduced by the crystal plasticity model.

Viruses use a plethora of mechanisms to evade immune responses. A new example is neutralization of the nuclear DNA cytosine deaminase APOBEC3B by the Epstein-Barr virus (EBV) ribonucleotide reductase subunit BORF2. Cryo-EM studies of APOBEC3B-BORF2 complexes reveal a large >1000 Å2 binding surface comprised of multiple structural elements from each protein, which effectively blocks the APOBEC3B active site from accessing single-stranded DNA substrates. Evolutionary optimization is suggested by unique insertions in BORF2 absent from other ribonucleotide reductases and preferential binding to APOBEC3B relative to the highly related APOBEC3A and APOBEC3G enzymes. An atomic understanding of this novel pathogen-host interaction may contribute to the development of drugs that block the interaction and liberate the natural antiviral activity of APOBEC3B.One-Sentence SummaryThese studies show how a conserved viral nucleotide metabolism protein is repurposed to inhibit a potent antiviral factor.

Combating the COVID-19 pandemic requires potent and low-cost therapeutics. We identified a series of single-domain antibodies (i.e., nanobody), Nanosota-1, from a camelid nanobody phage display library. Structural data showed that Nanosota-1 bound to the oft-hidden receptor-binding domain (RBD) of SARS-CoV-2 spike protein, blocking viral receptor angiotensin-converting enzyme 2 (ACE2). The lead drug candidate possessing an Fc tag (Nanosota-1C-Fc) bound to SARS-CoV-2 RBD ~3000 times more tightly than ACE2 did and inhibited SARS-CoV-2 pseudovirus ~160 times more efficiently than ACE2 did. Administered at a single dose, Nanosota-1C-Fc demonstrated preventive and therapeutic efficacy against live SARS-CoV-2 infection in both hamster and mouse models. Unlike conventional antibodies, Nanosota-1C-Fc was produced at high yields in bacteria and had exceptional thermostability. Pharmacokinetic analysis of Nanosota-1C-Fc documented an excellent in vivo stability and a high tissue bioavailability. As effective and inexpensive drug candidates, Nanosota-1 may contribute to the battle against COVID-19.

The bromodomain and extra terminal (BET) protein family recognizes acetylated lysines within histones and transcription factors using two N-terminal bromodomains, D1 and D2. The protein–protein interactions between BET bromodomains, acetylated histones, and transcription factors are therapeutic targets for BET-related diseases, including inflammatory disease and cancer. Prior work demonstrated that methylated-1,2,3-triazoles are suitable N-acetyl lysine mimetics for BET inhibition. Here we describe a structure–activity relationship study of triazole-based inhibitors that improve affinity, D1 selectivity, and microsomal stability. These outcomes were accomplished by targeting a nonconserved residue, Asp144 and a conserved residue, Met149, on BRD4 D1. The lead inhibitors DW34 and 26 have a BRD4 D1 K(d) of 12 and 6.4 nM, respectively. Cellular activity was demonstrated through suppression of c-Myc expression in MM.1S cells and downregulation of IL-8 in TNF-α-stimulated A549 cells. These data indicate that DW34 and 26 are new leads to investigate the anticancer and anti-inflammatory activity of BET proteins.

As many as five members of the APOBEC3 family of DNA cytosine deaminases are capable of inhibiting HIV-1 replication by deaminating viral cDNA cytosines and interfering with reverse transcription. HIV-1 counteracts restriction with the virally encoded Vif protein, which forms a hybrid ubiquitin ligase complex that directly binds APOBEC3 enzymes and targets them for proteasomal degradation. APOBEC3H (A3H) is unique among family members by dimerization through cellular and viral duplex RNA species. RNA binding is required for localization of A3H to the cytoplasmic compartment, for efficient packaging into nascent HIV-1 particles and ultimately for effective virus restriction activity. Here we compared wild-type human A3H and RNA binding–defective mutants to ask whether RNA may be a factor in the functional interaction with HIV-1 Vif. We used structural modeling, immunoblotting, live cell imaging, and split green fluorescence protein (GFP) reconstitution approaches to assess the capability of HIV-1 Vif to promote the degradation of wild-type A3H in comparison to RNA binding–defective mutants. The results combined to show that RNA is not strictly required for Vif-mediated degradation of A3H, and that RNA and Vif are likely to bind this single-domain DNA cytosine deaminase on physically distinct surfaces. However, a subset of the results also indicated that the A3H degradation process may be affected by A3H protein structure, subcellular localization, and differences in the constellation of A3H interaction partners, suggesting additional factors may also influence the fate and functionality of this host-pathogen interaction.

Background T lymphocytes play an important role in contact hypersensitivity. This study aims to explore the immunosuppressive activity of SBF-1, an analog of saponin OSW-1, against T lymphocytes in vitro and in vivo. Methods Proliferation of T lymphocytes from lymph nodes of mice was determined by MTT assay. Flow cytometry analysis was performed to assess T cell activation and apoptosis. Levels of cytokines were determined by PCR and ELISA. BALB/c mice were sensitized and challenged with picryl chloride and thickness of left and right ears were measured. Results SBF-1 effectively inhibited T lymphocytes proliferation induced by concanavalin A (Con A) or anti-CD3 plus anti-CD28 at a very low dose (10 nM) but exhibited little toxicity in non-activated T lymphocytes at concentrations up to 10 μM. In addition, SBF-1 inhibited the expression of CD25 and CD69, as well as he phosphorylation of AKT in Con A-activated T cells. SBF-1 also induced apoptosis of activated T cells. In addition, SBF-1 also downregulated the induction of the T cell cytokines, IL-2 and IFN-γ in a dose-dependent manner. Furthermore, SBF-1 significantly suppressed ear swelling and inflammation in a mouse model of picryl chloride-induced contact hypersensitivity. Conclusions Our findings suggest that SBF-1 has an unique immunosuppressive activity both in vitro and in vivo mainly through inhibiting T cell proliferation and activation. Its mechanism appears to be related to the blockage of AKT signaling pathway.

The amount and biodegradability of oil-degrading bacteria in seawater should be augmented to improve the biodegradation of marine petroleum hydrocarbons. This study investigated the degradation of ...

Development of energy storage materials with high energy density to fulfill the demand of next generation batteries is a blistering topic under immense debate. The Li–O2 battery is attracting much ...

The microstructure of the materials always has a great impact on their mechanical and thermal properties, and hence on the thermal shock resistance. It is, therefore, of great concern to investigate the correlation between the microstructure, mechanical/thermal properties, and thermal shock resistance of K-doped tungsten (W K) alloys that have recently generated considerable research interest in nuclear fusion materials community. Thus, in this study, a series of highly dense K-doped tungsten alloys with close grain size values (within a narrow range around 3.5 μm) but different K bubble characteristics, i.e., average bubble sizes (33.7 nm → 82.1 nm), were fabricated through controlling the K content (46/82/108/122/144 ppm). The thermal conductivity decreased, and the fracture toughness increased, with the increased K content, while the W K samples with K of 82 ppm presented the highest tensile strength and thermal shock resistance. The correlation between the microstructure, mechanical/thermal properties, and thermal shock resistance of K-doped tungsten alloys are then discussed. These investigations can help to design highly thermal shock resistant materials with high strength and toughness.

Poly(butylene succinate) (PBS) was hydrolyzed by two different enzymes: cutinase from Fusarium sp. and lipase from Candida antarctica (Lipozyme CALB). The differences between the PBS residues degraded over time were investigated with respect to their morphology, crystallinity and chemical structures. The degradation mechanism of PBS films using the two enzymes was further investigated by establishing a model. The results show that the PBS weight loss when degraded by cutinase was higher than when degraded by Lipozyme CALB under the same conditions. The PBS degradation mechanisms by cutinase and Lipozyme CALB occur by surface erosion and bulk erosion, respectively. Scanning electron microscopy shows that the diameter of pits formed after degradation by cutinase was approximately 3 times larger than those formed by Lipozyme CALB. The change in crystallinity is related to the type of enzyme used during the degradation process. Following Lipozyme CALB degradation, the degree of PBS crystallinity decreased with increasing time, leading to increased weight loss of the PBS films. Conversely, the PBS crystallinity was nearly unchanged following cutinase degradation. Powder X-ray diffraction indicates that the crystalline structure does not change during enzymatic hydrolysis. Fourier transform infrared spectroscopy confirms that the resulting structure of the degraded PBS is similar when treated with cutinase and Lipozyme CALB. Mass-spectrometric analysis suggested an endo-type action mode for both the enzymes.

The X-ray crystallographic structure of the mature form of subtilisin E-S7 (SES7) at 1.90 A resolution is reported here. Structural comparisons between the previously reported propeptide-subtilisin E complex (1SCJ) and our mature form subtilisin E-S7 (6O44) provide insight into active site adjustments involved in catalysis and specificity. To further investigate the protease substrate selectivity mechanism, we used SES7 to hydrolyze skim milk and analyzed the hydrolysates by LC-MS for peptide identification. The cleavage pattern suggests a high preference for proline at substrate P2 position. The results based on the peptide analysis are consistent with our structural observations, which is instrumental in future protein engineering by rational design. Furthermore, the ACE-inhibitor and NLN-inhibitor activity of the hydrolysates were determined to assess the utility of SES7 for further industrial applications; IC50-ACE = 67 ± 0.92 μg/mL and IC50-NLN = 263 ± 13 μg/mL.

Rechargeable non-aqueous Li-O2 battery is regarded as one of the most promising energy-storage technologies on account of its high energy density. It is believed that the rational design of three-dimensional (3D) architecture for catalyst is a key factor for the remarkable performance. Metal-organic frameworks (MOFs) derived materials possess excellent architecture, which is beneficial for Li-O2 batteries. In this work, ZIF-67 is used as precursor template and calcinated under different temperature to produce Co3O4 crystals. When the anneal treatment is under 350 °C, the derived Co3O4 nanocage holds the most complete skeleton, which provides better charge transfer ability as well as O2 and Li+ diffusion. Meanwhile, the Co3O4 nanocage owns more oxygen vacancies, offering more active sites. With the synergistic effect of nanocage structure and active sites, the Co3O4 nanocage stably delivers a large specific capacity of 15,500 mAh·g-1 as well as a long cycle-life of 132 cycles at limited discharge capacity of 1,000 mAh·g-1 under discharge/charge current density of 0.5 A·g-1.

W-K-TiC alloys with different titanium carbide concentrations (0.05, 0.1, 0.25, 0.5, 1, 2) wt.% were fabricated through Mechanical Alloying and Spark Plasma Sintering. The effects of the addition of nano-scaled TiC particles on the relative density, Vickers micro-hardness, microstructure, crystal information, thermal shock resistance, and tensile strength were investigated. It is revealed that the doped TiC nano-particles located at the grain boundaries. The relative density and Vickers micro-hardness of W-K-TiC alloys was enhanced with TiC addition and the highest Vickers micro-hardness is 731.55. As the TiC addition increased from 0.05 to 2 wt%, the room-temperature tensile strength raised from 141 to 353 MPa. The grain size of the W-K-TiC alloys decreased sharply from 2.56 μm to 330 nm with the enhanced TiC doping. The resistance to thermal shock damage of W-K-TiC alloys was improved slightly with the increased TiC addition. Keywords: Potassium-doped tungsten, Titanium carbide, Spark plasma sintering, Tensile strength, Thermal shock

At present, improvement in the degradation effect of diesel-degrading bacteria by combining them with surfactant-producing bacteria is an important way to efficiently repair the ecology of oil-cont...

Electrostimulated hydrolysis acidification (eHA) has been used as an efficient biological pre-treatment of refractory industrial wastewater. However, the effects of electrostimulation on the function and assembly of planktonic anaerobic sludge microbial communities are poorly understood. Using 16S rRNA gene and metagenomic sequencing, we investigated planktonic sludge microbial community structure, composition, function, assembly, and microbial interactions in response to electrostimulation. Compared with a conventional hydrolysis acidification (HA) reactor, the planktonic sludge microbial communities selected by electrostimulation promoted biotransformation of the azo dye Alizarin Yellow R. The taxonomic and functional structure and composition were significantly shifted upon electrostimulation with azo dyes degraders (e.g. Acinetobacter and Dechloromonas) and electroactive bacteria (e.g. Pseudomonas) being enriched. More microbial interactions between fermenters and decolorizing and electroactive bacteria, as well as fewer interactions between different fermenters evolved in the eHA microbial communities. Moreover, the decolorizing bacteria were linked to the higher abundance of genes encoding for azo- and nitro-reductases and redox mediator (e.g. ubiquinone) biosynthesis involved in the transformation of azo dye. Microbial community assembly was more driven by deterministic processes upon electrostimulation. This study offers new insights into the effects of electrostimulation on planktonic sludge microbial community function and assembly, and provides a promising strategy for the manipulation of anaerobic sludge microbiomes in HA engineering systems.

Despite conserved catalytic integration mechanisms, retroviral intasomes composed of integrase (IN) and viral DNA possess diverse structures with variable numbers of IN subunits. To investigate intasome assembly mechanisms, we employed the Rous sarcoma virus (RSV) IN dimer that assembles a precursor tetrameric structure in transit to the mature octameric intasome. We determined the structure of RSV octameric intasome stabilized by a HIV-1 IN strand transfer inhibitor using single particle cryo-electron microscopy. The structure revealed significant flexibility of the two non-catalytic distal IN dimers along with previously unrecognized movement of the conserved intasome core, suggesting ordered conformational transitions between intermediates that may be important to capture the target DNA. Single amino acid substitutions within the IN C-terminal domain affected intasome assembly and function in vitro and infectivity of pseudotyped RSV virions. Unexpectedly, 17 C-terminal amino acids of IN were dispensable for virus infection despite regulating the transition of the tetrameric intasome to the octameric form in vitro. We speculate that this region may regulate the binding of highly flexible distal IN dimers to the intasome core to form the octameric complex. Our studies reveal key steps in the assembly of RSV intasomes., Pandey, Bera, Shi et al. report the cryo-electron microscopy structure of the Rous sarcoma virus octameric intasome complex stabilized by a HIV-1 integrase strand transfer inhibitor. This new structure highlights the intrinsic flexibility of the distal integrase subunits and suggests that ordered conformational transitions occur within the conserved intasome core during the assembly process.

Spontaneous deamination of DNA cytosine and adenine into uracil and hypoxanthine, respectively, causes C to T and A to G transition mutations if left unrepaired. Endonuclease Q (EndoQ) initiates the repair of these premutagenic DNA lesions in prokaryotes by cleaving the phosphodiester backbone 5′ of either uracil or hypoxanthine bases or an apurinic/apyrimidinic (AP) lesion generated by the excision of these damaged bases. To understand how EndoQ achieves selectivity toward these structurally diverse substrates without cleaving undamaged DNA, we determined the crystal structures of Pyrococcus furiosus EndoQ bound to DNA substrates containing uracil, hypoxanthine, or an AP lesion. The structures show that substrate engagement by EndoQ depends both on a highly distorted conformation of the DNA backbone, in which the target nucleotide is extruded out of the helix, and direct hydrogen bonds with the deaminated bases. A concerted swing motion of the zinc-binding and C-terminal helical domains of EndoQ toward its catalytic domain allows the enzyme to clamp down on a sharply bent DNA substrate, shaping a deep active-site pocket that accommodates the extruded deaminated base. Within this pocket, uracil and hypoxanthine bases interact with distinct sets of amino acid residues, with positioning mediated by an essential magnesium ion. The EndoQ–DNA complex structures reveal a unique mode of damaged DNA recognition and provide mechanistic insights into the initial step of DNA damage repair by the alternative excision repair pathway. Furthermore, we demonstrate that the unique activity of EndoQ is useful for studying DNA deamination and repair in mammalian systems.

A bacterial cell infected with T4 phage rapidly establishes resistance against further infections by the same or closely related T-even-type bacteriophages – a phenomenon called superinfection exclusion. Here we show that one of the T4 early gene products and a periplasmic protein, Spackle, forms a stoichiometric complex with the lysozyme domain of T4 tail spike protein gp5 and potently inhibits its activity. Crystal structure of the Spackle-gp5 lysozyme complex shows that Spackle binds to a horseshoe-shaped basic patch surrounding the oligosaccharide-binding cleft and induces an allosteric conformational change of the active site. In contrast, Spackle does not appreciably inhibit the lysozyme activity of cytoplasmic T4 endolysin responsible for cell lysis to release progeny phage particles at the final step of the lytic cycle. Our work reveals a unique mode of inhibition for lysozymes, a widespread class of enzymes in biology, and provides a mechanistic understanding of the T4 bacteriophage superinfection exclusion., Ke Shi et al. perform a structural and biochemical characterization of the complex formed between Spackle, an early gene product of T4 and the gp5 lysozyme located in the tail of T4, thereby revealing a new mode of inhibition of the well-known lysozyme enzyme. This study provides structural insight into the phenomenon of superinfection exclusion, phenomenon by which bacterial cells infected with a T4 phage become resistant to secondary infections by the same or closely related phages.

Fluorescence pharmacokinetics can analyze the absorption, distribution, metabolism and other pharmacokinetic processes of fluorescence agents in biological tissues over time, which can provide more specific and quantitative physiological and pathological information for the evaluation of organ function. This paper is devoted to studying pharmacokinetics of indocyanine green (ICG) in healthy mice and mice with acute alcoholic liver injury based on a home-made dynamic diffuse fluorescence tomography system that possesses high sensitivity and large dynamic measurement range on account of digital lock-in-photon-counting technique. In this study, four-week-old Kunming mice were randomly divided into experimental and control groups. The time-varying distribution of ICG in mice was obtained by diffuse fluorescence tomography reconstruction, and the pharmacokinetic parameters were further extracted from the ICG concentration-time curve. The results showed that the dynamic diffuse fluorescence tomography system successfully captured the ICG metabolism process in mouse liver, and the ICG excretion rate demonstrated an obvious difference between healthy mice and the mice with acute alcoholic liver injury.

Bio-based and low-cost hybrid polyvinyl alcohol (PVA) and gelatin (Gel) hydrophilic macromolecular complex coated microspheres were prepared based on one-pot process, characterized, and applied as novel sorbent materials for the purification of trace aminoglycosides from complex matrices. PVA acts as a "rigid" component in the hybrid complex to enhance its mechanical properties, while Gel's "flexible" role is to improve the swelling properties of the hybrid complex in water. It is shown that hybrid PVA/Gel-functionalized sorbents are more efficient than the respective PVA or Gel sorbents since the presence of Gel increases the material selectivity for aminoglycosides, which is due to the specific interactions occurring between the targets and amino acid residues in the hybrid materials. Under the optimum conditions, material preparation and pretreatment processes were entirely carried out in single water system without toxic organic solvent. The detection limit (LOD) of spectinomycin, kanamycin, streptomycin and dihydrostreptomycin in honey were 0.811, 0.303, 0.168, 0.045 μg⋅kg-1 respectively. Linearity was obtained in the range of 20 to 2000 ug⋅kg-1, relative recovery yield up to 84.1-111.7% were obtained and matrix effect of all four aminoglycosides was within 100.8-107.6%. Intra-day and inter-day precision under four spiking levels (5, 200, 500 and 1000 ug⋅kg-1) were less than 10.9% (n=6) and 13.6% (n=3) respectively. In addition, the sorbents exhibited excellent reusability even after six recycles. This work demonstrates the potential of bio-based and low-cost hybrid polymer extraction platforms as promising bonded phase alternatives, in which eco-friendly and natural-based polymers can be used to improve the material selectivity and are conducive to the realization of "green chemistry".

Bromodomain and extra-terminal (BET) family proteins, BRD2-4 and T, are important drug targets; however, the biological functions of each bromodomain remain ill-defined. Chemical probes that selectively inhibit a single BET bromodomain are lacking, although pan inhibitors of the first (D1), and second (D2), bromodomain are known. Here, we develop selective BET D1 inhibitors with preferred binding to BRD4 D1. In competitive inhibition assays, we show that our lead compound is 9-33 fold selective for BRD4 D1 over the other BET bromodomains. X-ray crystallography supports a role for the selectivity based on reorganization of a non-conserved lysine and displacement of an additional structured water in the BRD4 D1 binding site relative to our prior lead. Whereas pan-D1 inhibitors displace BRD4 from MYC enhancers, BRD4 D1 inhibition in MM.1S cells is insufficient for stopping Myc expression and may lead to its upregulation. Future analysis of BRD4 D1 gene regulation may shed light on differential BET bromodomain functions.

Background Hepatocellular carcinoma (HCC) is the most common malignant tumor of the adult liver, exhibiting rapid progression and poor prognosis. Chlorogenic acid (CGA), a polyphenol, has several biological activities, including the suppression of liver cancer cell invasion and metastasis. Increased levels or alterations in the function of DNMT1 are associated with the inactivation of tumor suppressor genes. However, the CGA-affected DNMT1 expression mediated mechanism is still unclear. Methods The human hepatocellular carcinoma (HCC) HepG2 cells were treated with a positive control drug (5-AZA) or varying doses of CGA. DNA methyltransferase 1 (DNMT1) protein levels and other relevant proteins were evaluated using Western blotting and immunocytochemistry. Cell-cycle analysis was performed by flow cytometry-based PI staining, and cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The transwell invasion and wound healing assays were used to evaluate cell migration and invasion. In vivo proliferation of the HCC cells was detected. We investigated the expression of DNMT1, p53, p21, p-ERK, MMP-2, and MMP-9 in tumors using immunohistochemical analysis. Results Our results showed that CGA inhibited the proliferation, colony formation, invasion, and metastasis of HepG2 cells both in vitro and in vivo by down-regulating DNMT1 protein expression, which enhanced p53 and p21 activity, and resulting in a significant reduction in cell proliferation and metastasis. Moreover, CGA inactivated ERK1/2 and reduced MMP-2 and MMP-9 expression in HepG2 cells. Conclusions CGA can suppress liver cancer cell proliferation, invasion, and metastasis through several pathways. CGA could serve as a candidate chemopreventive agent for HCC.

The practicable application of microwave irradiation (MWI) on surface modification of carbon-based materials was greatly limited by severe arc discharging, since the arc discharging always appeared as an enemy to worsen and even damage these materials due to its strong etching function. Herein, the synthesized zinc-5,10,15,20-tetra(4-aminophenyl) porphyrin (ZnTAPc) was properly introduced to tame the arc discharging to become a useful friend by accepting the electrons excited from carbon fiber (CF) during MWI in H2O. By controlling MWI time, morphology transformation of ZnTAPc from microspheres (Monomer) to rice-like microparticle (J-type aggregation) could be tailored on ZnTAPc modified CF (MCF). The uniform dispersed layer of rice-like ZnTAPc showed strong chemical bonding and π-π conjugation with CF, which contributed to hydrophobic surface construction and obvious increase in interfacial strength of MCF-10/EP compared to CF-Commercial/EP. In particular, compared to CF-Commerical and CF-Commerical/EP, the hexane absorption capacity of MCF-10 was increased by 59.6 %, and the interfacial shearing strength (IFSS) of MCF-10/EP was increased by 86.6 %. It was showed that the tamed arc discharging was a kind of carbon material modification method with great potential in the field of composite materials and hydrophobic materials.

Poly(butylene succinate) (PBS), poly(butylene adipate) (PBA), and poly(butylene suberate) (PBSub) were synthesized and enzymatic hydrolysis was performed in the presence of cutinase. The residues degraded at different times were characterized with respect to their molecular, thermal, crystalline, and chemical structures. The results showed that the degradation behavior of the three polyesters highly depends on their CH2/CO ratio, morphology, and thermal characteristics. The three polyesters were nearly degraded after 24 h, and the degradation rates was in the order of PBSub > PBA > PBS. Gel permeation chromatography and end-group analysis confirmed that the mechanism of enzymatic hydrolysis changed from endo-type to exo-type. The analyses of differential scanning calorimetry, powder X-ray diffraction, and Fourier transform infrared spectroscopy indicated that the cutinase could simultaneously degrade the crystalline and amorphous regions of polyester films.

Summary: Double homeobox (DUX) transcription factors are unique to eutherian mammals. DUX4 regulates expression of repetitive elements during early embryogenesis, but misexpression of DUX4 causes facioscapulohumeral muscular dystrophy (FSHD) and translocations overexpressing the DUX4 double homeodomain cause B cell leukemia. Here, we report the crystal structure of the tandem homeodomains of DUX4 bound to DNA. The homeodomains bind DNA in a head-to-head fashion, with the linker making anchoring DNA minor-groove interactions and unique protein contacts. Remarkably, despite being tandem duplicates, the DUX4 homeodomains recognize different core sequences. This results from an arginine-to-glutamate mutation, unique to primates, causing alternative positioning of a key arginine side chain in the recognition helix. Mutational studies demonstrate that this primate-specific change is responsible for the divergence in sequence recognition that likely drove coevolution of embryonically regulated repeats in primates. Our work provides a framework for understanding the endogenous function of DUX4 and its role in FSHD and cancer. : Lee et al. determine the crystal structure of the facioscapulohumeral muscular dystrophy and cancer-associated transcription factor DUX4, bound to DNA. The structure gives insight into how the double homeodomain of DUX4, which is related by duplication of an ancestral homeodomain, has evolved different sequence specificities, uniquely in the primate lineage. Keywords: double homeobox, DUX, tandem homeodomains, facioscapulohumeral muscular dystrophy, FSHD, B cell leukemia, retrovirus-like elements, cleavage stage development, DNA sequence readout, crystal structure

Solid-state lithium batteries require the high energy density, easy processing and flexibility of electrolyte membranes. However, the low-temperature ionic conductivity and high interfacial impedance are still hindering the further application. Therefore, we developed the solution pouring method by using lithium bis(oxalate)borate (LiBOB) to prepare the PEO/LiBOB/LLZTO solid electrolyte for room-temperature solid-state lithium battery. Compared with the traditional LiPF6 as a lithium additive, the solid-state electrolytes with LiBOB as additive hold higher ionic conductivity and electrochemical window. Moreover, the fabricated solid-state LiFePO4|Li batteries own higher specific capacity and excellent cycle performance at room temperature (165.9 mAh g−1 with capacity retention of 84.6% after 100 cycles). The large volume of LiBOB could effectively decrease the crystallization state of PEO to improve the conductivity of Li+ ion by the increase in the amorphous region in the whole polymer electrolyte system. This study offers a feasible strategy of preparing solid electrolytes for room-temperature solid-state lithium battery with high performance.