1. D-Pinitol treatment induced the apoptosis in human leukemia MOLT-4 cells by improved apoptotic signaling pathway
- Author
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Xiaoli Gao, Keqian Shi, Weiwei Tan, Vishnu Priya Veeraraghavan, Surapaneni Krishna Mohan, Song Jin, Xuezhong Gu, Qi Wang, Xiangmei Yao, and Yanmei Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,Apoptosis ,01 natural sciences ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Western blot ,medicine ,MTT assay ,lcsh:QH301-705.5 ,chemistry.chemical_classification ,Reactive oxygen species ,Leukemia ,medicine.diagnostic_test ,Acridine orange ,MOLT-4 cells ,medicine.disease ,Molecular biology ,030104 developmental biology ,chemistry ,lcsh:Biology (General) ,Caspases ,Cancer cell ,D-pinitol ,Apoptotic signaling pathway ,General Agricultural and Biological Sciences ,010606 plant biology & botany - Abstract
Cancer is still remain as a global burden with the 18.1 million and 9.6 million new cases and mortlities, respectively estimated globally. Leukemia may arise at all ages varied from the infants to elders. In this exploration, we planned to evaluate the antiproliferative effect of D-pinitol on human leukemia MOLT-4 cells. Anticancer potential of D-pinitol was examined using MTT assay. Reactive oxygen species (ROS) generation was studied by fluorescence microscopic method using DCFH-DA staining. Apoptotic morphological alterations were determined by dual staining (acridine orange and ethidium bromide). Western blot and ELISA methods were employed to study apoptotic protein expression. D-pinitol treatment significantly induced cytotoxicity in human leukemia MOLT-4 cells. We observed that D-pinitol induces the generation of ROS in MOLT-4 cells. Further, we noticed that D-pinitol significantly induced apoptosis in a dosage dependent manner. Moreover, western blot and ELISA based analysis revealed that D-pinitol elevated the Bax, Caspase-3, Caspase-9 and attenuated the Bcl-2 expression in leukemic cancer cell. Our findings suggest that D-pinitol treatment induces the apoptosis in human leukemic cells by generating intracellular ROS and modulating apoptotic protein expression.
- Published
- 2020