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16 results on '"Orestis Faklaris"'

1. Ultrastructural and dynamic studies of the endosomal compartment in Down syndrome

Author

Alexandra Botté, Jeanne Lainé, Xavier Heiligenstein, Marie-Claude Potier, Graça Raposo, Gaëlle Fontaine, Isabelle Rivals, Anne-Sophie Rebillat, Dean Nizetic, Jack-Christophe Cossec, Etienne Morel, Laura Xicota, Pollyanna Goh, Orestis Faklaris, Amal Kasri, Institut du Cerveau et de la Moëlle Epinière = Brain and Spine Institute (ICM), Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Biologie Cellulaire et Cancer, Institut Curie [Paris]-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL), Equipe de Statistique Appliquée (UMRS 1158) (ESA), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Barts and the London Medical School, Queen Mary University of London (QMUL), Institut Jacques Monod (IJM (UMR_7592)), Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS), Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut Jérôme Lejeune, Nanyang Technological University [Singapour], Lee Kong Chian School of Medicine (LKCMedicine), Gestionnaire, Hal Sorbonne Université, Institut du Cerveau = Paris Brain Institute (ICM), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Sorbonne Université (SU)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Neurophysiologie Respiratoire Expérimentale et Clinique (UMRS 1158), Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)

Subjects
Tissue Fixation, Endosome, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Down syndrome, Induced Pluripotent Stem Cells, Endosomes, Immunofluorescence, Endocytosis, lcsh:RC346-429, Pathology and Forensic Medicine, law.invention, EEA1, Mice, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Microscopy, Electron, Transmission, law, Confocal microscopy, Early endosomes, medicine, Electron microscopy, Animals, Humans, Medicine [Science], Alzheimer’s Disease, Cholinergic neuron, Super-resolution microscopy, lcsh:Neurology. Diseases of the nervous system, 030304 developmental biology, 0303 health sciences, Microscopy, Confocal, medicine.diagnostic_test, Chemistry, Research, [SDV.NEU.NB] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Fibroblasts, Alzheimer's disease, Vitrification, Cell biology, Ultrastructure, Neurology (clinical), Down Syndrome, Electron microscope, Alzheimer’s disease, 030217 neurology & neurosurgery
Abstract

Enlarged early endosomes have been visualized in Alzheimer's disease (AD) and Down syndrome (DS) using conventional confocal microscopy at a resolution corresponding to endosomal size (hundreds of nm). In order to overtake the diffraction limit, we used super-resolution structured illumination microscopy (SR-SIM) and transmission electron microscopies (TEM) to analyze the early endosomal compartment in DS.By immunofluorescence and confocal microscopy, we confirmed that the volume of Early Endosome Antigen 1 (EEA1)-positive puncta was 13-19% larger in fibroblasts and iPSC-derived neurons from individuals with DS, and in basal forebrain cholinergic neurons (BFCN) of the Ts65Dn mice modelling DS. However, EEA1-positive structures imaged by TEM or SR-SIM after chemical fixation had a normal size but appeared clustered. In order to disentangle these discrepancies, we imaged optimally preserved High Pressure Freezing (HPF)-vitrified DS fibroblasts by TEM and found that early endosomes were 75% denser but remained normal-sized.RNA sequencing of DS and euploid fibroblasts revealed a subgroup of differentially-expressed genes related to cargo sorting at multivesicular bodies (MVBs). We thus studied the dynamics of endocytosis, recycling and MVB-dependent degradation in DS fibroblasts. We found no change in endocytosis, increased recycling and delayed degradation, suggesting a "traffic jam" in the endosomal compartment.Finally, we show that the phosphoinositide PI (3) P, involved in early endosome fusion, is decreased in DS fibroblasts, unveiling a new mechanism for endosomal dysfunctions in DS and a target for pharmacotherapy. National Research Foundation (NRF) Published version Marie-Claude Potier was supported by Fondation Vaincre Alzheimer (fellowship to AB), Fondation Jérôme Lejeune and Agence Nationale de laRecherche (ANR-10-IAIHU-06). Dean Nizetic was funded by Wellcome Trust (098330/Z/12/Z), NationalResearch Foundation Singapore (NMRC/CS-IRG/1438/2015), the WellcomeTrust Collaborative Award in Science 217199/Z/19/Z, and by the “Research Cooperability“ Programme of the Croatian Science Foundation (PZS-2019-02-4277). France-BioImaging infrastructure was supported by the French National Research Agency (ANR-10-INBS-04)..

Published
2020

2. RNA labelling in live plants reveals single cell transcriptional dynamics: application to phosphate signaling

Author

Edouard Bertrand, Laura Cuyas, Marie-Christine Thibaud, Eric Maréchal, Nathalie Pochon, Sahar Hani, Florian Mueller, Laurent Nussaume, Hélène Javot, Rémy Merret, James Whelan, Pascale David, Orestis Faklaris, and David Secco

Subjects
chemistry.chemical_compound, medicine.anatomical_structure, Chemistry, Labelling, Dynamics (mechanics), Cell, medicine, RNA, Phosphate, Cell biology
Abstract

Plants are sessile organisms constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest generation RNA imaging system and combined it with microfluidics to visualize transcriptional regulation in living Arabidopsis plants. This enabled quantitative measurements of the transcriptional activity of single loci in single cells, real time and changing environmental conditions. Using phosphate responsive genes as model, we found that active genes displayed high transcription initiation rates (∼3s) and frequently clustered together in endoreplicated cells. We observed gene bursting and large allelic differences in single cells, revealing that at steady-state, intrinsic noise dominated extrinsic variations. Moreover, we established that transcriptional repression triggered in roots by phosphate, a crucial macronutrient limiting plant development, occurred with unexpected fast kinetics (∼minutes) and striking heterogeneity between neighboring cells. Access to single cell RNA polymerase II dynamics within live plants will benefit future studies of signaling processes.

Published
2021

3. Embryogenesis of the peristaltic reflex

Author

Cécile Jacques, Lucas Langlois, Chloé Guedj, Nicolas Chevalier, Orestis Faklaris, Nicolas Dacher, Matière et Systèmes Complexes (MSC (UMR_7057)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), and Institut Jacques Monod (IJM (UMR_7592))

Subjects
0301 basic medicine, Embryo, Nonmammalian, Physiology, [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Myogenic contraction, Chick Embryo, Tetrodotoxin, Enteric Nervous System, 03 medical and health sciences, symbols.namesake, Mice, 0302 clinical medicine, Reflex, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Premovement neuronal activity, Animals, ComputingMilieux_MISCELLANEOUS, Migrating motor complex, Zebrafish, Peristalsis, Chemistry, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Smooth muscle contraction, Embryo, Mammalian, Interstitial cell of Cajal, Cell biology, Intestines, 030104 developmental biology, embryonic structures, symbols, Enteric nervous system, Nitric Oxide Synthase, Gastrointestinal Motility, 030217 neurology & neurosurgery, Neuroscience, Muscle Contraction
Abstract

Key points Neurogenic gut movements start after longitudinal smooth muscle differentiation in three species (mouse, zebrafish, chicken), and at E16 in the chicken embryo. The first activity of the chicken enteric nervous system is dominated by inhibitory neurons. The embryonic enteric nervous system electromechanically couples circular and longitudinal spontaneous myogenic contractions, thereby producing a new, rostro-caudally directed bolus transport pattern: the migrating motor complex. The response of the embryonic gut to mechanical stimulation evolves from a symmetric, myogenic response at E12, to a neurally mediated, polarized, descending inhibitory, 'law of the intestine'-like response at E16. High resolution, whole-mount 3D reconstructions are presented of the enteric nervous system of the chicken embryo at the neural-control stage E16 with the iDISCO+ tissue clarification technique. Abstract Gut motility is a complex transport phenomenon involving smooth muscle, enteric neurons, glia and interstitial cells of Cajal. Because these different cells differentiate and become active at different times during embryo development, studying the ontogenesis of motility offers a unique opportunity to 'time-reverse-engineer' the peristaltic reflex. Working on chicken embryo intestinal explants in vitro, we found by spatio-temporal mapping and signal processing of diameter and position changes that motility follows a characteristic sequence of increasing complexity: (1) myogenic circular smooth muscle contractions from E6 to E12 that propagate as waves along the intestine, (2) overlapping and independent, myogenic, low-frequency, bulk longitudinal smooth muscle contractions around E14, and (3) tetrodotoxin-sensitive coupling of longitudinal and circular contractions by the enteric nervous system as from E16. Inhibition of nitric oxide synthase neurons shows that the coupling consists in nitric oxide-mediated relaxation of circular smooth muscle when the longitudinal muscle layer is contracted. This mechanosensitive coupling gives rise to a directional, cyclical, propagating bolus transport pattern: the migrating motor complex. We further reveal a transition to a polarized, descending, inhibitory reflex response to mechanical stimulation after neuronal activity sets in at E16. This asymmetric response is the elementary mechanism responsible for peristaltic transport. We finally present unique high-resolution 3D reconstructions of the chicken enteric nervous system at the neural-control stage based on confocal imaging of iDISCO+ clarified tissues. Our study shows that the enteric nervous system gives rise to new peristaltic transport patterns during development by coupling spontaneous circular and longitudinal smooth muscle contraction waves.

Published
2019

4. Post-production modifications of murine mesenchymal stem cell (mMSC) derived extracellular vesicles (EVs) and impact on their cellular interaction

Author

Sarah Le Saux, Simon George, Orestis Faklaris, Patrick Bron, Hanna Aarrass, Jean Armengaud, Justine Bertrand-Michel, Jean-Marie Devoisselle, Emeric Dubois, Philippe Legrand, Joséphine Lai-Kee-Him, Marie Morille, Guylaine Miotello, Joël Chopineau, Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Centre de Biochimie Structurale [Montpellier] (CBS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biochimie des Systèmes Perturbés (LBSP), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire Innovations technologiques pour la Détection et le Diagnostic (LI2D), Service de Pharmacologie et Immunoanalyse (SPI), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Médicaments et Technologies pour la Santé (MTS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MetaToul Lipidomics, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-MetaboHUB-MetaToul, MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Université de Toulouse (UT)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-BioCampus (BCM), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), BioCampus (BCM), Université de Nîmes (UNIMES), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plateau MetaToul-LIPIDOMIQUE = MetaToul-Lipidomics, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-MetaToul-MetaboHUB, Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Génopole Toulouse Midi-Pyrénées [Auzeville] (GENOTOUL), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-MetaboHUB-MetaToul, Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Ecole Nationale Vétérinaire de Toulouse (ENVT), CCSD, Accord Elsevier, Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM)-MetaToul-MetaboHUB, and Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1)-Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1)

Subjects
[SDV]Life Sciences [q-bio], Endocytic cycle, Biophysics, Bioengineering, Endocytosis Pathway, 02 engineering and technology, Biomaterials, Extracellular Vesicles, Mice, 03 medical and health sciences, Drug Delivery Systems, Caveolae, Animals, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Liposome, Chemistry, Vesicle, Mesenchymal stem cell, Mesenchymal Stem Cells, 021001 nanoscience & nanotechnology, Microvesicles, Cell biology, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics, [SDV] Life Sciences [q-bio], Freeze Drying, Mechanics of Materials, Liposomes, Drug delivery, Ceramics and Composites, 0210 nano-technology
Abstract

International audience; In regards to their key role in intercellular communication, extracellular vesicles (EVs) have a strong potential as bio-inspired drug delivery systems (DDS). With the aim of circumventing some of their well-known issues (production yield, drug loading yield, pharmacokinetics), we specifically focused on switching the biological vision of these entities to a more physico-chemical one, and to consider and fine-tune EVs as synthetic vectors. To allow a rational use, we first performed a full physico-chemical (size, concentration, surface charge, cryoTEM), biochemical (western blot, proteomics, lipidomics, transcriptomics) and biological (cell internalisation) characterisation of murine mesenchymal stem cell (mMSC)-derived EVs. A stability study based on evaluating the colloidal behaviour of obtained vesicles was performed in order to identify optimal storage conditions. We evidenced the interest of using EVs instead of liposomes, in regards to target cell internalisation efficiency. EVs were shown to be internalised through a caveolae and cholesterol-dependent pathway, following a different endocytic route than liposomes. Then, we characterised the effect of physical methods scarcely investigated with EVs (extrusion through 50 nm membranes, freeze-drying, sonication) on EV size, concentration, structure and cell internalisation properties. Our extensive characterisation of the effect of these physical processes highlights their promise as loading methods to make EVs efficient delivery vehicles.

Published
2020

5. MEIOB and SPATA22 resemble RPA subunits and interact with the RPA complex to promote meiotic recombination

Author

Xavier Veaute, Gabriel Livera, Carole Saintomé, Orestis Faklaris, Duquenne C, Martini E, Devanshi Jain, Didier Busso, Ribeiro J, Scott Keeney, Raphael Guerois, Pauline Dupaigne, and Petrillo C

Subjects
0303 health sciences, biology, DNA repair, Chemistry, Protein subunit, 030302 biochemistry & molecular biology, RAD51, Helicase, complex mixtures, Cell biology, Chromosome segregation, enzymes and coenzymes (carbohydrates), 03 medical and health sciences, Meiosis, biology.protein, DMC1, Homologous recombination, 030304 developmental biology
Abstract

Homologous recombination is a conserved DNA repair process mandatory for chromosome segregation during meiosis. RPA, a ubiquitous complex essential to recombination, is thought to play a similar role during mitotic and meiotic recombination. MEIOB, a meiosis-specific factor with unknown molecular function, ressembles a RPA subunit. Here we usein vivoapproaches to show that in mouse spermatocytes, DMC1 and RAD51 appear to be normally loaded in the absence of MEIOB but are prematurely lost from unrepaired recombination sites. This loss correlates with an accumulation of the BLM helicase on meiotic chromosomes. We also show that MEIOB alters the immunodetection of RPA subunits at meiotic recombination sites. Using electron microscopy and purified proteins, we demonstrate that the MEIOB-SPATA22 complex associates with and modifies the conformation of RPA-coated ssDNA. Finally, we identify structural homology between MEIOB, SPATA22 and RPA subunits, and show that MEIOB and SPATA22 interact through C-terminal OB-fold containing domains (OBCDs) like RPA subunits. Moreover, MEIOB and SPATA22 cooperate to interact with RPA through their OBCDs. Our results suggest that MEIOB, SPATA22 and RPA work together to ensure proper processing of meiotic recombination intermediates.

Published
2018

6. Ultrabright and Fluorogenic Probes for Multicolor Imaging and Tracking of Lipid Droplets in Cells and Tissues

Author

Tkhe Kyong Fam, Thierry Galli, Andrey S. Klymchenko, Pichandi Ashokkumar, Lydia Danglot, Mayeul Collot, Orestis Faklaris, Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Biophotonique et Pharmacologie (CNRS UMR 7213), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biophotonique et Pharmacologie - UMR 7213 (LBP), Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))

Subjects
Models, Molecular, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Indoles, multicolor imaging, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Biochemistry, Catalysis, KB Cells, chemistry.chemical_compound, Mice, Colloid and Surface Chemistry, Lipid droplet, Microscopy, Organelle, Moiety, Animals, Humans, Merocyanine, Benzopyrans, Fluorescent Dyes, tissue imaging, communication, Chemistry, Optical Imaging, Nile red, dioxaborine, General Chemistry, Lipid Droplets, 021001 nanoscience & nanotechnology, Fluorescence, Two-Photon Excitation imaging, 0104 chemical sciences, Mice, Inbred C57BL, Adipose Tissue, Liver, Microscopy, Fluorescence, Biophysics, 0210 nano-technology, Intracellular
Abstract

Lipid droplets (LDs) are intracellular lipid-rich organelles that regulate the storage of neutral lipids and were recently found to be involved in many physiological processes, metabolic disorders, and diseases including obesity, diabetes, and cancers. Herein we present a family of new fluorogenic merocyanine fluorophores based on an indolenine moiety and a dioxaborine barbiturate derivative. These so-called StatoMerocyanines (SMCy) fluoresce from yellow to the near-infrared (NIR) in oil with an impressive fluorescence enhancement compared to aqueous media. Additionally, SMCy display remarkably high molar extinction coefficients (up to 390 000 M–1 cm–1) and high quantum yield values (up to 100%). All the members of this new family specifically stain the LDs in live cells with very low background noise. Unlike Nile Red, a well-known lipid droplet marker, SMCy dyes possess narrow absorption and emission bands in the visible, thus allowing multicolor imaging. SMCy proved to be compatible with fixation and le...

Published
2018

7. Fluorescent-Based Strategies to Investigate G Protein-Coupled Receptors: Evolution of the Techniques to a Better Understanding

Author

Amandine Falco, Julie Perroy, Thierry Durroux, Elise Goyet, Jurriaan M. Zwier, Bernard Mouillac, Orestis Faklaris, Joyce Heuninck, Jean-Philippe Pin, Laboratoire de Photonique Quantique et Moléculaire (LPQM), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Physiologie & médecine expérimentale du Cœur et des Muscles [U 1046] (PhyMedExp), Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Montpellier (UM), Cisbio Bioassays, Institut de Génomique Fonctionnelle (IGF), and Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Chemistry, G protein, Energy transfer, Computational biology, Physiological responses, Protein–protein interaction, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Identification (biology), [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Signal transduction, Receptor, 030217 neurology & neurosurgery, ComputingMilieux_MISCELLANEOUS, G protein-coupled receptor
Abstract

G protein-coupled receptors are key proteins in the regulation of most of the physiological responses. Their conformations are generally oscillating between inactive and active forms leading to the activation of no, a few, or many signaling pathways. Although receptors can spontaneously adopt these various conformations, their interactions with ligands, other G protein-coupled receptors, or intracellular proteins (G proteins, arrestins, etc.) can stabilize one of these conformations, leading to specific cellular responses. The identification of the partners interacting with the G protein-coupled receptors and the dynamics of these interactions is therefore crucial to fully understand receptor functioning. Although it is crucial, it remains nevertheless ambitious and difficult to achieve this goal. In the last two decades, various technical strategies have been developed to investigate molecular complexes and their dynamics. In this review, we will focus on recent technological breakthroughs in fluorescent-based techniques and their impact on the understanding of G protein-coupled receptor functioning. We will give particular attention to resonance energy transfer-based strategies, their advantages, and drawbacks and to other microscopy based techniques which are efficient to investigate stability, mobility, and dynamics of molecular complexes at the cell surface.

Published
2017

8. Fluorescent ligands to investigate GPCR binding properties and oligomerization

Author

Bernard Mouillac, Thierry Durroux, Jean-Philippe Pin, Orestis Faklaris, Amadine Falco, Martin Cottet, and Eric Trinquet

Subjects
Ligand, Plasma protein binding, Ligands, Biochemistry, Oxytocin receptor, Fluorescence, Receptors, G-Protein-Coupled, chemistry.chemical_compound, Biopolymers, Förster resonance energy transfer, chemistry, Fluorescence Resonance Energy Transfer, Fluorescein, Receptor, Fluorescent Dyes, Protein Binding, G protein-coupled receptor
Abstract

Fluorescent ligands for GPCRs (G-protein-coupled receptors) have been synthesized for a long time but their use was usually restricted to receptor localization in the cell by fluorescent imaging microscopy. During the last two decades, the emergence of new fluorescence-based strategies and the concomitant development of fluorescent measurement apparatus have dramatically widened the use of fluorescent ligands. Among the various strategies, TR (time-resolved)-FRET (fluorescence resonance energy transfer) approaches exhibit an interesting potential to study GPCR interactions with various partners. We have derived various sets of ligands that target different GPCRs with fluorophores, which are compatible with TR-FRET strategies. Fluorescent ligands labelled either with a fluorescent donor (such as europium or terbium cryptate) or with a fluorescent acceptor (such as fluorescein, dy647 or Alexa Fluor® 647), for example, kept high affinities for their cognate receptors. These ligands turn out to be interesting tools to develop FRET-based binding assays. We also used these fluorescent ligands to analyse GPCR oligomerization by measuring FRET between ligands bound to receptor dimers. In contrast with FRET strategies, on the basis of receptor labelling, the ligand-based approach we developed is fully compatible with the study of wild-type receptors and therefore with receptors expressed in native tissues. Therefore, by using fluorescent analogues of oxytocin, we demonstrated the existence of oxytocin receptor dimers in the mammary gland of lactating rats.

Published
2013

9. Photoluminescent nanodiamonds: Comparison of the photoluminescence saturation properties of the NV color center and a cyanine dye at the single emitter level, and study of the color center concentration under different preparation conditions

Author

Jacques Botsoa, François Treussart, Thierry Sauvage, Jean-François Roch, and Orestis Faklaris

Subjects
Materials science, Photoluminescence, Synthetic diamond, Mechanical Engineering, Nanoparticle, Diamond, Nanotechnology, General Chemistry, engineering.material, Fluorescence, Electronic, Optical and Magnetic Materials, law.invention, chemistry.chemical_compound, chemistry, Nanocrystal, law, Materials Chemistry, engineering, Electrical and Electronic Engineering, Cyanine, Nanodiamond
Abstract

Nitrogen-Vacancy (NV) color centers in diamond nanoparticles (NanoDiamonds, NDs) have a great number of applications, in particular in quantum information processing and as cellular fluorescent labels. In this work we compare the photoluminescence properties of a single NV color center embedded in a 30 nm diameter nanodiamond to that of a single organic fluorescent molecule. We also study the photoluminescence properties of NDs prepared under different conditions in order to find the optimal parameters to achieve a high fluorescence yield, which is still an open question in the case of nanocrystals compared to bulk diamond. The results of this study are of interest for the applications of small and bright diamond nanoparticles in nanophotonics and biology.

Published
2010

10. Multicolor time-resolved Förster resonance energy transfer microscopy reveals the impact of GPCR oligomerization on internalization processes

Author

Amandine Falco, Michel Laget, Bernard Mouillac, Thierry Durroux, Orestis Faklaris, Eric Trinquet, Brice Villier, Jean-Philippe Pin, Martin Cottet, and Jurriaan M. Zwier

Subjects
Receptors, Vasopressin, media_common.quotation_subject, CHO Cells, Biochemistry, Oligomer, Time-Lapse Imaging, Receptors, G-Protein-Coupled, chemistry.chemical_compound, Molecular dynamics, Cricetulus, Cricetinae, Microscopy, Genetics, Fluorescence Resonance Energy Transfer, Animals, Humans, Internalization, Molecular Biology, media_common, G protein-coupled receptor, HEK 293 cells, Reproducibility of Results, Endocytosis, Cell biology, Molecular network, Förster resonance energy transfer, HEK293 Cells, chemistry, Microscopy, Fluorescence, Protein Multimerization, Biotechnology
Abstract

Identifying the interacting partners and the dynamics of the molecular networks constitutes the key point in understanding cellular processes. Different methods often based on energy transfer strategies have been developed to examine the molecular dynamics of protein complexes. However, these methods suffer a couple of drawbacks: a single complex can be studied at a time, and its localization and tracking cannot generally be investigated. Here, we report a multicolor time-resolved Forster resonance energy transfer microscopy method that allows the identification of up to 3 different complexes simultaneously, their localization in cells, and their tracking after activation. Using this technique, we studied GPCR oligomerization and internalization in human embryonic kidney 293 cells. We definitively show that receptors can internalize as oligomers and that receptor coexpression deeply impacts oligomer internalization processes.

Published
2014

11. New Fluorescent Strategies Shine Light on the Evolving Concept of GPCR Oligomerization

Author

Thierry Durroux, Jean-Philippe Pin, Martin Cottet, Orestis Faklaris, and Eric Trinquet

Subjects
Förster resonance energy transfer, Single-particle tracking, Chemistry, Artificial systems, Fluorescent protein, Context (language use), Nanotechnology, Computational biology, Fluorescence, G protein-coupled receptor
Abstract

GPCR oligomerization has been a matter of intense research these last years. FRET and BRET methods have paved the way to a generalized concept of potential GPCR oligomerization in artificial systems (transfected cell lines). More recently, the use of fluorescent ligands compatible with time-resolved FRET studies has opened the possibility of GPCR oligomerization study in their native context and brought evidence of their existence. Furthermore, recent applications of original fluorescence techniques are unveiling new information on the dynamics that govern these complexes and are changing the way we see GPCR oligomeric structures.

Published
2012

12. Superresolution imaging of albumin-conjugated fluorescent nanodiamonds in cells by stimulated emission depletion

Author

Huan-Cheng Chang, Yung Kuo, Jui-Hung Hsu, Be-Ming Chang, Yan-Kai Tzeng, and Orestis Faklaris

Subjects
Microscopy, Confocal, Chemistry, Albumin, STED microscopy, Nanoparticle, Nanotechnology, Serum Albumin, Bovine, General Medicine, General Chemistry, Conjugated system, Endocytosis, Fluorescence, Catalysis, Nanodiamonds, Microscopy, Fluorescence, Lactalbumin, Animals, Humans, Cattle, Stimulated emission, Nanodiamond, Fluorescent Dyes, HeLa Cells
Published
2010

13. Comparison of the photoluminescence properties of semiconductor quantum dots and non-blinking diamond nanoparticles. Observation of the diffusion of diamond nanoparticles in living cells

Author

Jean-Paul Boudou, Damien Garrot, Patrick A. Curmi, Thierry Sauvage, Orestis Faklaris, Fran c cois Treussart, Vandana Joshi, Laboratoire de Photonique Quantique et Moléculaire (LPQM), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec-École normale supérieure - Cachan (ENS Cachan), Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de mécanique des sols, structures et matériaux (MSSMat), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec, Conditions Extrêmes et Matériaux : Haute Température et Irradiation (CEMHTI), Centre National de la Recherche Scientifique (CNRS)-Université d'Orléans (UO), Financement européen: 'Nano4Drugs' (contrat LSHB-2005-CT-019102), Financement région 'Ile de France'(C'Nano IdF): projet 'Biodiam'., NaDia (contrat ANR-2007-PNANO-045),NaDia (contrat ANR-2007-PNANO-045), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-07-NANO-0045,NADIA,Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant(2007), CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Université d'Orléans (UO)-Centre National de la Recherche Scientifique (CNRS), Treussart, François, and Nanosciences et nanotechnologies - Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant - - NADIA2007 - ANR-07-NANO-0045 - NANO - VALID

Subjects
[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics], Photoluminescence, Materials science, Fluorophore, FOS: Physical sciences, Nanoparticle, quantum dots, 02 engineering and technology, 010402 general chemistry, photostability, 01 natural sciences, chemistry.chemical_compound, [PHYS.QPHY]Physics [physics]/Quantum Physics [quant-ph], Molecule, [PHYS.QPHY] Physics [physics]/Quantum Physics [quant-ph], Condensed Matter - Materials Science, Quantum Physics, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], business.industry, Materials Science (cond-mat.mtrl-sci), 021001 nanoscience & nanotechnology, Photobleaching, [PHYS.COND.CM-MS] Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Atomic and Molecular Physics, and Optics, 0104 chemical sciences, chemistry, Quantum dot, nanodiamonds, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Optoelectronics, Particle, biomarker, photoluminescence, Quantum Physics (quant-ph), 0210 nano-technology, business, Excitation, Optics (physics.optics), Physics - Optics
Abstract

International audience; Long-term observations of photoluminescence at the single-molecule level were until recently very difficult, due to the photobleachingof organic fluorophore molecules. Although inorganic semiconductor nanocrystals can overcome this difficulty showing very low photobleachingyield, they suffer from photoblinking. A new marker has been recently introduced, relying on diamond nanoparticles containingphotoluminescent color centers. In this work we compare the photoluminescence of single quantum dots (QDs) to the one of nanodiamondscontaining a single-color center. Contrary to other markers, photoluminescent nanodiamonds present a perfect photostability andno photoblinking. At saturation of their excitation, nanodiamonds photoluminescence intensity with a single NV-color center is only threetimes smaller than one of the single QDs. Moreover, the bright and stable photoluminescence of nanodiamonds allows wide field observationsof single nanoparticles motion. We demonstrate the possibility of recording the trajectory of such single particle in culture cells.

Published
2009

14. Photoluminescent diamond nanoparticles for cell labeling: study of the uptake mechanism in mammalian cells

Author

Alain Thorel, Theano Irinopoulou, Hugues A. Girard, Jean-Paul Boudou, Céline Gesset, Patrick A. Curmi, François Treussart, Patrick Tauc, Mohamed Senour, Jean-Charles Arnault, Orestis Faklaris, Vandana Joshi, Faklaris, Orestis, Nanosciences et nanotechnologies - Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant - - NADIA2007 - ANR-07-NANO-0045 - NANO - VALID, Laboratoire de Photonique Quantique et Moléculaire (LPQM), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut du Fer à Moulin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biologie et de Pharmacologie Appliquée (LBPA), École normale supérieure - Cachan (ENS Cachan)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Intégration des Systèmes et des Technologies (LIST (CEA)), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Centre des Matériaux (CDM), Mines Paris - PSL (École nationale supérieure des mines de Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), 'Nano4Drugs' (contract LSHB-2005-CT-019102), ANR-07-NANO-0045,NADIA,Nanocristaux de diamant, photoluminescence et croissance de couches ultraminces de diamant(2007), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec-École normale supérieure - Cachan (ENS Cachan), Laboratoire d'Intégration des Systèmes et des Technologies (LIST), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Centre des Matériaux (MAT), Centre National de la Recherche Scientifique (CNRS)-PSL Research University (PSL)-MINES ParisTech - École nationale supérieure des mines de Paris, 'NaDia' (contract ANR-2007-PNANO-045),'NaDia' (contract ANR-2007-PNANO-045), and MINES ParisTech - École nationale supérieure des mines de Paris

Subjects
[PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics], Macromolecular Substances, Surface Properties, Molecular Conformation, FOS: Physical sciences, General Physics and Astronomy, Nanoparticle, 02 engineering and technology, 010402 general chemistry, Endocytosis, 01 natural sciences, Clathrin, diamond, Materials Testing, Humans, Nanotechnology, endocytosis, General Materials Science, Particle Size, chemistry.chemical_classification, [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], Staining and Labeling, biology, Vesicle, Biomolecule, General Engineering, Colocalization, biolabel, 021001 nanoscience & nanotechnology, Nanostructures, 0104 chemical sciences, Endocytic vesicle, chemistry, Transmission electron microscopy, Luminescent Measurements, biology.protein, Biophysics, nanoparticles, photoluminescence, Crystallization, 0210 nano-technology, Optics (physics.optics), Physics - Optics, HeLa Cells
Abstract

International audience; Diamond nanoparticles (nanodiamonds) have been recently proposed as new labels for cellular imaging. For small nanodiamonds (size

Published
2009

15. Diamond nanoparticles as photoluminescent nanoprobes

Author

Thierry Sauvage, Orestis Faklaris, Jean-Paul Boudou, Vandana Joshi, Patrick A. Curmi, François Treussart, Serge Huant, Aurélien Cuche, Jean-François Roch, Yannick Sonnefraud, Laboratoire de Photonique Quantique et Moléculaire (LPQM), Centre National de la Recherche Scientifique (CNRS)-CentraleSupélec-École normale supérieure - Cachan (ENS Cachan), NOF - Nano-Optique et Forces, Institut Néel (NEEL), Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), Centre d'Études et de Recherches par Irradiation (CERI), Centre National de la Recherche Scientifique (CNRS), Structure et activité des biomolécules normales et pathologiques (SABNP), Université d'Évry-Val-d'Essonne (UEVE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Biogéochimie et écologie des milieux continentaux (Bioemco), Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Paris (ENS Paris), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Nano-Optique et Forces (NOF), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS), Nano-Optique et Forces (NEEL - NOF), École normale supérieure - Paris (ENS-PSL), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Pierre et Marie Curie - Paris 6 (UPMC)-AgroParisTech-Centre National de la Recherche Scientifique (CNRS)

Subjects
Photoluminescence, General Physics and Astronomy, markers, Nanotechnology, 02 engineering and technology, Living cell, engineering.material, 01 natural sciences, law.invention, color centers, Optical microscope, diamond, law, near-field optics, 0103 physical sciences, 010302 applied physics, Physics, chemistry.chemical_classification, Biomolecule, Near-field optics, Diamond, 021001 nanoscience & nanotechnology, chemistry, [PHYS.COND.CM-GEN]Physics [physics]/Condensed Matter [cond-mat]/Other [cond-mat.other], engineering, Diamond nanoparticles, 0210 nano-technology
Abstract

Proceedings of COLOQ'10 - 10ème COlloque sur les Lasers et l'Optique Quantique, Grenoble, July 2007; We present preliminary results showing the potential of diamond nanoparticles with size

Published
2007

16. Diamond nanoparticles as photoluminescent nanoprobes for biology

Author

Alexandra Elli, Thierry Sauvage, François Treussart, Jean-Paul Boudou, Orestis Faklaris, Vandana Joshi, Jean-François Roch, and Patrick A. Curmi

Subjects
chemistry.chemical_classification, Materials science, Photoluminescence, chemistry, Biomolecule, engineering, Diamond, Diamond nanoparticles, Nanotechnology, Living cell, engineering.material
Abstract

The present work reports our attempt in developing diamond nanoparticles with size

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