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Background: The most used denture base is heat-cured acrylic resin. It can causes denture stomatitis. The action taken to prevent denture stomatitis is by cleaning the denture. Chemical denture cleansers can reduce surface hardness, increase the degree of surface roughness and may cause discoloration of acrylic resin surfaces. Alternative natural denture cleanser is used to minimize this effects. The natural denture cleanser is using 12,5% Basil leave and 25% Mauli banana stem extracts which are known to have antifungal effectiveness against Candida albicans. The combination of these two extracts is expected to have better effectiveness against Candida albicans without changing the nature of the acrylic resin base in surface roughness, hardness and color. Objective: This study aimed to analyze the comparison in surface hardness, surface roughness, and discoloration of a heat-cured acrylic resin which immersed in 12.5% Basil leave and 25% Mauli banana stem extracts with alkaline peroxide and distilled water. Methods: This study was a true experimental study with pre and posttest with control group design. Study on surface hardness used 24 cylindrical type heat-cured acrylic resin samples with a diameter of 30 mm and 5 mm thickness. Study on surface roughness used 24 acrylic resin rectangular 65 x 10 x 3.3 mm. Study on discoloration used 24 acrylic resin sized 15mm in diameter x 2mm thick; n=8/group. The study used 3 treatment groups: 12.5% Basil leaves and 25% Mauli banana stem extracts, alkaline peroxide, and distilled water. Hardness testing was measured using Vickers Hardness Testing and surface roughness measurement was performed using surface roughness tester. The value of discoloration was measured before and after the immersion for five days with the CIELab system using digital analysis tools. Result: The mean of the decrease in surface hardness value of heat-cured acrylic resin which was immersed in extract solution, alkaline peroxide, distilled water are 1.40, 1.38, and 0.60 respectively. The mean results of surface roughness increase in 12,5% Basil leave and 25% Mauli banana stem extracts, alkaline peroxide, and distilled water group were 0.0613 µm, 0.0663 µm, and 0.0425 µm respectively. Statistical tests on discoloration showed significant differences (p

Background: Aggressive periodontitis is a disease that is mostly suffered by young patients with minimal local factors. The prevalence of this disease is approximately 8% in Indonesia, where Aggregatibacter actinomycetemcomitans bacteria are presented as dominant bacteria in this disease. Aggregatibacter actinomycetemcomitans is a gram-negative bacterium that is sensitive to flavonoids. Ramania leaves are natural substances which contain flavonoids. Flavonoids in ramania leaves can be applied as an antibacterial substance. Objectives: The objectives of this research are to determine and analyze the minimum inhibitory concentration and the minimum bactericidal concentration of flavonoids extract from ramania leaves at the concentration of 0.1%, 0.3%, and 0.5%, and analyze the antibacterial effectiveness on Aggregatibacter actinomycetemcomitans. Methods: True experimental design with pre and post-test with control group design was applied in this study. Antibacterial test using broth and agar dilution method was performed in a total of 5 treatment groups with 6 repetitions. Results: All exract at 0.1%, 0.3% and 0.5% concentration demonstrated the ability to inhibit bacterial growth, and the minimum bactericidal concentration was found at 0.5% concentration. Result test for One way ANNOVA on minimum inhibitory concentration and Kruskal wallis test on minimum bactericidal concentration exhibited significant value of less than 0.05 presenting significant differences in some groups. Conclusion: Minimum inhibitory concentration of ramania leaves flavonoid extract is effective on Aggregatibacter actinomycetemcomitans at the concentration of 0.1% and minimum bactericidal concentration of ramania leaves flavonoid extract is more effective at the concentration of 0.5%. Keywords: Aggregatibacter Actinomycetemcomitans, Aggressive Periodontitis, Dilution Method, Flavonoids, Ramania Leaves.

Background : Stimulation of inflammatory cells such as polymorphonuclear cells (PMN) will initiate to release free radicals in destroying bacteria. Excessive free radicals can damage cells in the body. Antioxidants as one of the body's defense systems will neutralize existing free radicals. Chitosan from Haruan scales in addition to being antimicrobial turned out to also be antioxidants. Objective: To know the antioxidant potential of chitosan from haruan (Channa striata) scales. Method s : This study is a pure experimental (true experimental design) with a post-test only with control group design using two treatment groups, with quantitative tests to calculate the amount of antioxidant activity of chitosan from fish scales using DPPH radical reduction method. The first treatment is 4, 6, 8 and 10 ppm standard ascorbic acid concentration with DPPH solution as positive control, and the second treatment is 200, 250, 300 and 350 ppm chitosan from Haruan scales concentration with DPPH solution. Results: The results showed that chitosan from Haruan fish scales have antioxidant activity with 50.513% percentage of inhibition at a maximum concentration of 350 ppm with an IC50 value of 356.98 ppm. The results of the independent T-test showed that there was a significant difference between the inhibition percentage of chitosan from haruan fish scales and the inhibition percentage of ascorbic acid (p = 0,000) (p