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37 results on '"The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS)"'

1. Horizontal buried channels in monocrystalline silicon

Author

B. Le Pioufle, P. Guil, D. Gaudin, Olivier Bonnaud, Matthieu Denoual, O. De Sagazan, Kervella, Katell, Institut d'Électronique et des Technologies du numéRique (IETR), Université de Nantes (UN)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS), Nantes Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), electronique, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Laboratoire de mécanique des fluides (LMF), École Centrale de Nantes (ECN)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS), Université de Nantes (UN)-Université de Rennes 1 (UR1), and The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Fabrication, Materials science, Silicon, Annealing (metallurgy), Microfluidics, chemistry.chemical_element, Hardware_PERFORMANCEANDRELIABILITY, 02 engineering and technology, Integrated circuit, Epitaxy, 01 natural sciences, law.invention, Application-specific integrated circuit, law, 0103 physical sciences, Hardware_INTEGRATEDCIRCUITS, Electronic engineering, Electrical and Electronic Engineering, ComputingMilieux_MISCELLANEOUS, 010302 applied physics, Microelectromechanical systems, business.industry, 021001 nanoscience & nanotechnology, Condensed Matter Physics, Electronic, Optical and Magnetic Materials, chemistry, Hardware and Architecture, Optoelectronics, 0210 nano-technology, business
Abstract

This paper reports horizontal buried channels in monocristalline silicon. Those channels are a few microns in width and height and several hundred microns long. The main applications for those buried channels are microfluidic networks for MEMS devices and cooling systems for integrated circuits. Their fabrication is based on integrated circuit standard processes such as selective monocristalline epitaxial growth and high temperature annealing. The major interest of the method is its compatibility with integrated circuit manufacturing technology.

Published
2006
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2. EGF repeats of epidermal growth factor‑like domain 7 promote endothelial cell activation and tumor escape from the immune system

Author

Sébastien Pinte, Chantal Havet, Gaëlle Villain, Suzanne Delfortrie, Fabrice Soncin, Virginie Mattot, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Mécanismes de tumorigenèse et thérapies ciblées, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Institut Pasteur de Lille, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
EGF Family of Proteins, Cancer Research, EGF-like domain, T cell, Down-Regulation, [SDV.CAN]Life Sciences [q-bio]/Cancer, Natural killer cell, Mice, 03 medical and health sciences, 0302 clinical medicine, Epidermal growth factor, medicine, Animals, ComputingMilieux_MISCELLANEOUS, Cell Proliferation, 030304 developmental biology, Mice, Inbred BALB C, 0303 health sciences, Cell adhesion molecule, Cell growth, Chemistry, Calcium-Binding Proteins, Endothelial Cells, General Medicine, Cell biology, Endothelial stem cell, Disease Models, Animal, medicine.anatomical_structure, Oncology, Immune System, 030220 oncology & carcinogenesis, Female, Tumor Escape, EGFL7, Endothelium, Vascular
Abstract

International audience; The tumor blood vessel endothelium forms a barrier that must be crossed by circulating immune cells in order for them to reach and kill cancer cells. Epidermal growth factor‑like domain 7 (Egfl7) represses this immune infiltration by lowering the expression levels of leukocyte adhesion receptors on the surface of endothelial cells. However, the protein domains involved in these properties are not completely understood. Egfl7 is structurally composed of the predicted EMI‑, EGF‑ and C‑terminal domains. The present study aimed to investigate the roles of these different domains in tumor development by designing retroviruses coding for deletion mutants and then infecting 4T1 breast cancer cell populations, which consequently overexpressed the variants. By performing in vitro soft‑agar assays, it was found that Egfl7 and its deletion variants did not affect cell proliferation or anchorage‑independent growth. When 4T1 cells expressing either the wild‑type Egfl7 protein or Egfl7 domain variants were implanted in mice, Egfl7 expression markedly promoted tumor development and deletion of the EGF repeats decreased the tumor growth rate. By contrast, deleting any other domain displayed no significant effect on tumor development. The overexpression of Egfl7 also decreased T cell and natural killer cell infiltration in tumors, as determined by immunofluorescence staining of tumor sections, whereas deletion of the EGF repeats inhibited this effect. Reverse transcription‑quantitative PCR analysis of the mechanisms involved revealed that deleting the EGF repeats partially restored the expression levels of vascular cell adhesion molecule 1 and E‑selectin, which were suppressed by overexpression of Egfl7 in endothelial cells in vitro. This resulted in a higher number of lymphocytes bound to HUVEC expressing Egfl7‑ΔEGF compared with HUVEC expressing wild‑type Egfl7, as assessed by fluorescent‑THP‑1 adhesion assays onto endothelial cells. Overall, the present study demonstrated that the EGF repeats may participate in the protumoral and anti‑inflammatory effects of Egfl7.

Published
2021
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3. Direct measurement of the mechanical properties of a chromatin analog and the epigenetic effects of para-sulphonato-calix[4]arene

Author

Anthony W. Coleman, Yannick Tauran, Gregoire Perret, Hiroyuki Fujita, Laurent Jalabert, Momoko Kumemura, Mehmet C. Tarhan, Dominique Collard, Florent Perret, Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Thermodynamique des solutions et des polymères (TSP), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS), Centre de Recherche Universitaire Lorrain d'Histoire (CRULH), Université de Lorraine (UL), and The University of Tokyo (UTokyo)

Subjects
0301 basic medicine, Optical Tweezers, [SDV]Life Sciences [q-bio], lcsh:Medicine, Microscopy, Atomic Force, Proof of Concept Study, Article, Histones, 03 medical and health sciences, Methyllysine, chemistry.chemical_compound, 0302 clinical medicine, Nucleosome, Animals, Chromosomes, Artificial, Epigenetics, lcsh:Science, Regulation of gene expression, Multidisciplinary, biology, Chemistry, lcsh:R, [CHIM.MATE]Chemical Sciences/Material chemistry, Bacteriophage lambda, Chromatin, 3. Good health, Cell biology, Nucleosomes, 030104 developmental biology, Histone, DNA, Viral, biology.protein, Nucleic Acid Conformation, Cattle, lcsh:Q, Histone deacetylase, 030217 neurology & neurosurgery, DNA
Abstract

International audience; By means of Silicon Nano Tweezers (SNTs) the effects on the mechanical properties of λ-phage DNA during interaction with calf thymus nucleosome to form an artificial chromatin analog were measured. At a concentration of 100 nM, a nucleosome solution induced a strong stiffening effect on DNA (1.1 N m −1). This can be compared to the effects of the histone proteins, H1, H2A, H3 where no changes in the mechanical properties of DNA were observed and the complex of the H3/H4 proteins where a smaller increase in the stiffness is observed (0.2 N m −1). Para-sulphonato-calix[4]arene, SC4, known for epigenetic activity by interacting specifically with the lysine groups of histone proteins, was studied for its effect on an artificial chromatin. Using a microfluidic SNT device, SC4 was titrated against the artificial chromatin, at a concentration of 1 mM in SC4 a considerable increase in stiffness, 15 N m −1 , was observed. Simultaneously optical microscopy showed a physical change in the DNA structure between the tips of the SNT device. Electronic and Atomic Force microscopy confirmed this structural rearrangement. Negative control experiments confirmed that these mechanical and physical effects were induced neither by the acidity of SC4 nor through nonspecific interactions of SC4 on DNA. With the recent and better knowledge on how gene regulation occurs and after intense debate in the academic community 1 , epigenetics has been commonly defined as "the study of changes in gene function that are mitoti-cally and/or meiotically heritable and that do not entail a change in DNA sequence" 2. Over the past years, epigenetics has been shown to play a central role in many different key functions at the cellular level, including differentiation, replication, and gene transcription 3. In a larger context, its contribution in physiological disorders has been revealed in cardio vascular illnesses, mental disorders and various cancers 4. Despite its inheritable character, epigenetics paradoxically possesses a reversible nature 5 that can be illustrated through its two main covalent modifications either directly on DNA with the methylation of cytosine nucleic bases or indirectly with various post translational modifications on proteins interacting with DNA such as histone proteins or other proteins called writers, erasers or readers 6. New therapeutic treatments are expected to target and reverse these modifications by inactivating the enzymes responsible for the deregulation of specific genes 7. Some inhibitors have been already approved by FDA, such as Azacitidine and Decitabine as DNA methyltrans-ferase inhibitors for the treatment of myelodysplastic syndrome 8 and Vorinostat and Romidepsin as inhibitors of histone deacetylase for the treatment of peripheral T-cell lymphoma 9. A new class of DNA artificial binders that directly block the methyllysine reading functions of reader enzymes in charge of conveying the methylation signal downstream has recently arisen as promising epigenetic drugs 10 .

Published
2019
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4. Self-Deformable Flexible Mems Tweezer Made of Poly (Vinylidene Fluoride) /Ionic Liquid Gel with Electrical Measurement Capability

Author

Akio Higo, Guilhem Larrieu, Yoshiho Ikeuchi, Takafumi Yamaguchi, Agnes Tixier-Mita, Yoshio Mita, Timothée Levi, Ken Saito, Kei Misumi, Gilgueng Hwang, Naoto Usami, Atsushi Toyokura, Shimpei Ono, Graduate School of Engineering [The Univ of Tokyo] (UTokyo), The University of Tokyo (UTokyo), Central Research Institute of Electrical Power Industry, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Centre de Nanosciences et de Nanotechnologies (C2N), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Équipe Matériaux et Procédés pour la Nanoélectronique (LAAS-MPN), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), Nihon University, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, and Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Cantilever, Fabrication, Micromechanical devices, Silver, electrical observation, Ionic bonding, Self-deformable cantilever, Bending, chemistry.chemical_compound, soft gripping, [SPI]Engineering Sciences [physics], flexible actuator, Composite material, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Electrodes, ionic liquid, Microelectromechanical systems, PVDF-TrFE, Wiring, silver nanowire, Liquids, chemistry, Electrode, Ionic liquid, Solvents, Actuators, Voltage
Abstract

We demonstrated a self-bending flexible tweezer capable of simultaneous mechanical handling and electrical measurement. A soft cantilever of $2\ \text{mm} \times 8\ \text{mm} \times 75\ \mu \mathrm{m}$ bent by itself, and electrical signal observation through touched surface was successful. The working voltage (1.5 V) was the lowest, and normalized strain was one of the highest values as compared to world's similar devices, to our best knowledge. Unique fabrication process of flexible cantilever with novel ionic polymer-metal composites (IPMCs) was developed. The material was poly (vinylidene fluoride-co-trifluoroethylene) (PVDF-TrFE) and ionic liquid (IL) by use of acetone solvent, and silver nanowire for electrodes. The IL of 50 wt% gave the largest 7 mm bending displacement at + 1.5 V DC . The cantilever with the above actuator touched on a gold test surface, then electrical observation of alternating voltage of 700 mV (1 kHz and 4 kHz) was confirmed.

Published
2021
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5. ENGINEERING MICROSCALE BIOMIMETIC HYDROSCAFFOLD FOR DYNAMIC THREE-DIMENSIONAL MODELING OF PANCREATIC CANCER

Author

Elodie Vandenhaute, Nathalie Maubon, Audrey Vincent, Joseph de Saxce, Zied Souguir, Lucie Dercourt, Vincent Senez, Frédérick de Miollis, Charles Poiraud, Isabelle Van Seuningen, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 (CANTHER), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), HCS-Pharma Loos [Lille] (HCS-PL), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Centre de Recherche pour la Conservation des Collections (CRCC), Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - IEMN (Univ. Lille, CNRS, Centrale Lille, Junia, UPHF) (IEMN - UMR 8520), Ecole Centrale de Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Laboratoire de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), Laboratoire d'Études Rurales (LER), Université Lumière - Lyon 2 (UL2)-Isara, HCS Pharma, Lille, France, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U1172 Inserm - U837 (JPArc), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Lille Nord de France (COMUE)-Université de Lille, Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Bio-Micro-Electro-Mechanical Systems - IEMN (BIOMEMS - IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), and SENEZ, Vincent

Subjects
[PHYS]Physics [physics], BIOMIMESYS, Tumor microenvironment, organ-on-a-chip, Materials science, [SPI] Engineering Sciences [physics], hyaluronan hydroscaffold, Cancer, Pancreatic cancer, medicine.disease, Interstitial fluid flow, 3. Good health, FOLFIRINOX, chemistry.chemical_compound, [SPI]Engineering Sciences [physics], chemistry, Hyaluronic acid, medicine, Cancer research, Hepatic stellate cell, tumor microenvironment, Folfirinox Protocol, Microscale chemistry, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; Pancreatic cancer (PC) is a deadly cancer for which no diagnostic or prevention plan currently exists since this cancer is asymptomatic in its early development. The very specific and complex tumor microenvironment of PC is responsible for the unsuccessful delivery of therapeutics molecules to tumor cells. For high throughput testing of new molecules, there is a crucial need for more accurate preclinical model. For the first time, we integrated in a microfluidic system 5 major features of PC microenvironment: i) type-I collagen (CI), ii) hyaluronic acid (HA), iii) pancreatic stellate cells (SC), iv) interstitial fluid flow and v) nutrient and oxygen gradients. We showed long 3D culture (30 days). Furthermore, we implemented, thanks to the perfusion capability, the actual FOLFIRINOX protocol to assess the IC50 and we showed that dynamic conditions induce increased chemoresistance compared to 3D static.

Published
2021
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6. Assessing the impact of ambient fabrication temperature on the performance of planar CH3NH3PbI3 perovskite solar cells

Author

Lionel Hirsch, Dario M. Bassani, Sylvain Chambon, Zuzanna Molenda, Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences Moléculaires (ISM), and Université Montesquieu - Bordeaux 4-Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Fabrication, Vapor pressure, business.industry, Band gap, Chemistry, Energy conversion efficiency, 02 engineering and technology, Electroluminescence, 010402 general chemistry, 021001 nanoscience & nanotechnology, 7. Clean energy, 01 natural sciences, 0104 chemical sciences, law.invention, [SPI.MAT]Engineering Sciences [physics]/Materials, Inorganic Chemistry, law, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, Optoelectronics, Crystallization, 0210 nano-technology, Absorption (electromagnetic radiation), business, Perovskite (structure)
Abstract

International audience; The ambient fabrication temperature inside the glove box is found to play an important role in determining the overall power conversion efficiency in solar cells based on CH3NH3PbI3 (MAPI) hybrid organic-inorganic perovskites. We find that a variation of the ambient temperature of only 10°C during the fabrication process has a crucial impact on both the reproducibility and the efficiency of the devices. Atomic Force Microscopy, XRD, UV-Vis absorption and electroluminescence have been carried out to investigate the origin of this behavior. We conclude that the partial vapor pressure variation of the solvent impacts the crystallization process inducing an increase density of traps within the bandgap of the perovskite.

Published
2021
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7. Microwell-based pancreas-on-chip model enhances genes expression and functionality of rat islets of Langerhans

Author

Yannick Tauran, Cécile Legallais, Mathieu Danoy, Marie Shinohara, Teru Okitsu, Amal Essaouiba, Yasuyuki Sakai, Eric Leclerc, Rachid Jellali, Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo), Biomécanique et génie biomédical (BIM), and Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, endocrine system, endocrine system diseases, Cell Survival, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Gene Expression, Biochemistry, Glucagon, Models, Biological, Tissue Culture Techniques, 03 medical and health sciences, Islets of Langerhans, 0302 clinical medicine, Endocrinology, Downregulation and upregulation, Insulin Secretion, Microchip Analytical Procedures, medicine, Glucose homeostasis, Animals, Insulin, Rats, Wistar, Molecular Biology, Pancreas, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, biology, Tissue Scaffolds, Chemistry, Pancreatic islets, Glucagon secretion, Cell biology, Rats, Insulin receptor, medicine.anatomical_structure, Glucose, 030220 oncology & carcinogenesis, biology.protein
Abstract

Organ-on-chip technology is a promising tool for investigating physiological in vitro responses in drug screening development, and in advanced disease models. Within this framework, we investigated the behavior of rat islets of Langerhans in an organ-on-chip model. The islets were trapped by sedimentation in a biochip with a microstructure based on microwells, and perfused for 5 days of culture. The live/dead assay confirmed the high viability of the islets in the biochip cultures. The microfluidic culture leads to upregulation of mRNA levels of important pancreatic islet genes: Ins1, App, Insr, Gcgr, Reg3a and Neurod. Furthermore, insulin and glucagon secretion were higher in the biochips compared to the Petri conditions after 5 days of culture. We also confirmed glucose-induced insulin secretion in biochips via high and low glucose stimulations leading to high/low insulin secretion. The high responsiveness of the pancreatic islets to glucagon-like peptide 1 (GLP-1) stimulation in the biochips was reflected by the upregulation of mRNA levels of Gcgr, Reg3a, Neurog3, Ins1, Ins2, Stt and Glp-1r and by increased insulin secretion. The results obtained highlighted the functionality of the islets in the biochips and illustrated the potential of our pancreas-on-chip model for future pancreatic disease modeling and anti-diabetic drugs screening.

Published
2020
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8. Characterization of liver zonation‐like transcriptomic patterns in HLCs derived from hiPSCs in a microfluidic biochip environment

Author

Taketomo Kido, Eric Leclerc, Atsushi Miyajima, Benedikt Scheidecker, Stéphane Poulain, Sachi Kato, Charles Plessy, Mathieu Danoy, Myriam Lereau-Bernier, Yasuyuki Sakai, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Centre de Ressources Biologiques - [Nancy] (CRB Nancy), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), RIKEN Center for Life Science Technologies (RIKEN CLST), RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), The University of Tokyo (UTokyo), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Biomécanique et génie biomédical (BIM), Centre National de la Recherche Scientifique (CNRS), Leclerc, Eric, Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I

Subjects
[SDV]Life Sciences [q-bio], Cellular detoxification, Induced Pluripotent Stem Cells, SMAD, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, Mesenchymal–epithelial transition, Humans, Epithelial–mesenchymal transition, Transcription factor, Cells, Cultured, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Tissue Engineering, Chemistry, Wnt signaling pathway, Cell Differentiation, Microfluidic Analytical Techniques, Cell biology, Nanostructures, [SDV] Life Sciences [q-bio], Liver, 030220 oncology & carcinogenesis, Hepatocytes, Biotechnology
Abstract

The liver zonation is an important phenomenon characterized by a gradient of several functions along the liver acinus. However, this gradient remains difficult to reproduce in in-vitro conditions, making the obtention of an in-vitro method to recapitulate the liver zonation a challenging issue. In this study, we evaluated the spatial evolution of the transcriptome profile of human induced pluripotent stem cells (hiPSCs) differentiated toward hepatocytes-like cells (HLCs) phenotype in a microfluidic biochip environment. Cells collected at the inlet of the biochip, where the oxygen concentration is higher, were identified by the expression of genes involved in metabolic pathways related to cellular reorganization and cell proliferation. Cells collected in the middle and at the outlet of the biochips, where oxygen concentrations are lower, were characterized by the upregulation of genes involved in cellular detoxification processes (CYP450), PPAR signaling or arginine biosynthesis. A subset of 16 transcription factors (TFs) was extracted and identified as upstream regulators to HNF1A and PPARA. These TFs are also known as regulators to target genes engaged in the Wnt/βcatenin pathway, in the TGFβ/BMP/SMAD signaling, in the transition between epithelial mesenchymal transition (EMT) and mesenchymal epithelial transition (MET), in the homeostasis of lipids, bile acids and carbohydrates homeostasis, in drug metabolism, in the estrogen processing and in the oxidative stress response. Overall, the analysis allowed to confirm a partial zonation-like pattern in hiPSCs-derived HLCs in the microfluidic biochip environment. These results provide important insights into the reproduction of liver zonation in-vitro for a better understanding of the phenomenon.

Published
2020
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9. Integration of metabolomic and transcriptomic profiles of hiPSCs-derived hepatocytes in a microfluidic environment

Author

Taketomo Kido, Yasuyuki Sakai, Rachid Jellali, Charles Plessy, Françoise Gilard, Stéphane Poulain, Mathieu Danoy, Eric Leclerc, Atsushi Miyajima, Sachi Kato, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), RIKEN Center for Life Science Technologies, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo, Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Centre de Ressources Biologiques - [Nancy] (CRB Nancy), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), RIKEN Center for Life Science Technologies (RIKEN CLST), RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), The University of Tokyo (UTokyo), ANR-16-RHUS-0005,iLite,iLite(2016), and Institut National de la Recherche Agronomique (INRA)-Université Paris-Sud - Paris 11 (UP11)-Université Paris Diderot - Paris 7 (UPD7)-Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0303 health sciences, Environmental Engineering, Chemistry, [SDV]Life Sciences [q-bio], Biomedical Engineering, Bioengineering, Pentose phosphate pathway, Fatty acid beta-oxidation, 01 natural sciences, Cell biology, Transcriptome, Spermidine, 03 medical and health sciences, chemistry.chemical_compound, Metabolomics, In vivo, 010608 biotechnology, Glycolysis, Biochip, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Biotechnology
Abstract

International audience; The differentiation of human induced pluripotent stem cells (hiPSCs) into functional hepatocytes has the potential to solve the shortage of human primary liver cells and would be of use in drug screening. In this frame, we developed a hiPSCs maturation strategy in microfluidic biochips, using a liver-on-chip approach, a promising technology mimicking in vivo physiology. Hepato-like tissues differentiated in biochips presented advanced liver features, including ALB and CYP3A4 expressing cells. The metabolomics and transcriptomics profiles of hepato-likes cells differentiated either in biochips or Petri dishes were integrated to compare their functionalities. The multi-omics analysis revealed 41 metabolites and 302 genes differentially expressed. Overall, biochip environment lead to higher degree of hepatic differentiation demonstrated by an increase in the metabolic production of lipids, fatty acids and biliary acids, which was confirmed at the transcriptome level by the modulation of expression for genes involved in related signaling pathways. This observation was correlated with higher production of fructose in biochips, together with down-regulation of genes engaged in glycolysis. In parallel, increased activity of the Krebs cycle, pentose phosphate shuffle, and fatty acid beta oxidation was observed in tissues cultured in Petri. Besides, the modulation of nitrogen metabolism was observed in transcriptomic data and confirmed by an intense production of glutamine, putrescine and creatinine and by the higher consumption of spermidine measured in Petri.

Published
2020
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10. Spatiotemporal control of DNA-based chemical reaction network via electrochemical activation in microfluidics

Author

Guillaume Gines, Yannick Rondelez, Yannick Coffinier, Alexis Vlandas, Ievgen Kurylo, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Gulliver (UMR 7083), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Bio-Micro-Electro-Mechanical Systems - IEMN (BIOMEMS - IEMN), I.K. would like to thank the CNRS and the region Haut de France for financial support. A.V. would like to thank the region Haut de France for support for funding the NanoTrami (Project Emergent)., and Renatech Network

Subjects
0301 basic medicine, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Dynamical systems theory, Computer science, Distributed computing, Microfluidics, lcsh:Medicine, Molecular systems, Electrochemistry, Network topology, Chemical reaction, Article, law.invention, Autocatalysis, 03 medical and health sciences, chemistry.chemical_compound, DNA computing, law, Microfluidic channel, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, lcsh:Science, Electrodes, Multidisciplinary, lcsh:R, DNA, Electrochemical Techniques, 030104 developmental biology, chemistry, Scalability, Key (cryptography), lcsh:Q, Gold, Unconventional computing, Biosensor
Abstract

In recent years, DNA computing frameworks have been developed to create dynamical systems which can be used for information processing. These emerging synthetic biochemistry tools can be leveraged to gain a better understanding of fundamental biology but can also be implemented in biosensors and unconventional computing. Most of the efforts so far have focused on changing the topologies of DNA molecular networks or scaling them up. Several issues have thus received little attention and remain to be solved to turn them into real life technologies. In particular, the ability to easily interact in real-time with them is a key requirement. The previous attempts to achieve this aim have used microfluidic approaches, such as valves, which are cumbersome. We show that electrochemical triggering using DNA-grafted micro-fabricated gold electrodes can be used to give instructions to these molecular systems. We demonstrate how this approach can be used to release at specific times and locations DNA- based instructions. In particular, we trigger reaction-diffusion autocatalytic fronts in microfluidic channels. While limited by the stability of the Au-S bond, this easy to implement, versatile and scalable technique can be used in any biology laboratory to provide new ways to interact with any DNA-based computing framework.

Published
2018
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11. An interphase microfluidic culture system for the study of ex vivo intestinal tissue

Author

Sadia Benamrouz Vanneste, Colette Creusy, Eric Viscogliosi, Vincent Senez, Anthony Treizeibré, Gabriela Certad, Martha Baydoun, Baptiste Delaire, Anthony Mouray, Jérôme Follet, Lucie Dercourt, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Institut Supérieur d'Agriculture de Lille (ISA), Université catholique de Lille (UCL), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Bio-Micro-Electro-Mechanical Systems - IEMN (BIOMEMS - IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Yncréa Hauts-de-France, Smart and Sustainable Cities (SCC), Université Catholique de Lille - Faculté de gestion, économie et sciences (FGES), Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Institut Catholique de Lille (ICL), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Groupement des Hôpitaux de l'Institut Catholique de Lille (GHICL), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Groupe Hospitalier de l'Institut Catholique de Lille (GHICL), Réseau International des Instituts Pasteur (RIIP), This research was funded by grants from the 'Les Hauts de France' region, the 'Métropole Européenne de Lille' city, the Catholic University of Lille, the Pasteur Institute of Lille, the Centre National de la Recherche Scientifique (CNRS), The Institute for Research on Cancer in Lille (IRCL), the LIMMS/SMMIL-E research program and the University of Lille., Renatech Network, IEMN, Collection, Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), YNCREA Hauts France, Partenaires INRAE, Unité de Recherche Smart and Sustainable Cities [Lille], and Faculté de gestion, économie et sciences [UCL, Lille] (FGES)

Subjects
[SPI] Engineering Sciences [physics], lcsh:Mechanical engineering and machinery, [SDV]Life Sciences [q-bio], Microfluidics, 02 engineering and technology, ex vivo microfluidic tissue culture, Article, 03 medical and health sciences, [SPI]Engineering Sciences [physics], Biopsy, medicine, lcsh:TJ1-1570, Electrical and Electronic Engineering, intestinal tract model, 030304 developmental biology, 0303 health sciences, Microchannel, medicine.diagnostic_test, long term viability maintenance, Chemistry, Mechanical Engineering, 021001 nanoscience & nanotechnology, Control and Systems Engineering, organotypic tissue model, Interphase, Full thickness, 0210 nano-technology, Perfusion, Ex vivo, Biomedical engineering, Explant culture
Abstract

International audience; Ex vivo explant culture models offer unique properties to study complex mechanisms underlying tissue growth, renewal, and disease. A major weakness is the short viability depending on the biopsy origin and preparation protocol. We describe an interphase microfluidic culture system to cultivate full thickness murine colon explants which keeps morphological structures of the tissue up to 192 h. The system was composed of a central well on top of a porous membrane supported by a microchannel structure. The microfluidic perfusion allowed bathing the serosal side while preventing immersion of the villi. After eight days, up to 33% of the samples displayed no histological abnormalities. Numerical simulation of the transport of oxygen and glucose provided technical solutions to improve the functionality of the microdevice.

Published
2020
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12. 3D micro fractal pipettes for capillary based robotic liquid handling

Author

Gilgueng Hwang, Beom Joon Kim, A. Harouri, Yoshio Mita, Dominique Decanini, Centre de Nanosciences et de Nanotechnologies (C2N), Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), and The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS)

Subjects
010302 applied physics, chemistry.chemical_classification, Pressure drop, Materials science, Microchannel, Capillary action, Pipette, Nanotechnology, Polymer, Substrate (printing), 01 natural sciences, 010305 fluids & plasmas, [SPI.AUTO]Engineering Sciences [physics]/Automatic, Fractal, chemistry, 0103 physical sciences, Porosity, Instrumentation
Abstract

International audience; Miniaturized and mobile liquid handling devices are essential elements to biological or clinical applications. This will innovate the conventional liquid handling methods such as manual or automated pipetting systems. Here, we propose the micro fractal pipette as the candidate device for this objective. It is made of epoxy polymer and printed by innovative 3D nanoprinting technology based on two-photon absorption polymerization with sub-micrometer resolution. We demonstrated the efficient liquid handling performance by using the micro fractal pipette between the source droplet and the target hydrogel substrate. This is due to the high porosity (78%) and the 8.5 times larger cavity surface area compared to the full pyramid. The biomimetic inner cavity microchannel networks contribute to the low pressure drop. The proposed micro fractal pipette could also innovate the versatile and miniaturized liquid handling system, promising to various biological or clinical applications

Published
2020
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13. Analysis of the transcription factors and their regulatory roles during a step-by-step differentiation of induced pluripotent stem cells into hepatocyte-like cells

Author

Bertrand-David Segard, Sachi Kato, Atsushi Miyajima, Marie Shinohara, Charles Plessy, Taketomo Kido, Mathieu Danoy, Stéphane Poulain, Myriam Lereau-Bernier, Eric Leclerc, Yannick Tauran, Yasuyuki Sakai, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), RIKEN Center for Life Science Technologies (RIKEN CLST), RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), RIKEN Center for Integrative Medical Sciences [Yokohama] (RIKEN IMS), Center for International Research on Integrative Biomedical Systems [University of Tokyo] (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo (UTokyo)-The University of Tokyo (UTokyo), Laboratory of Stem Cell Therapy Center for Experimental Medicine, and The University of Tokyo (UTokyo)-Institute of Medical Sciences

Subjects
[SDV]Life Sciences [q-bio], Induced Pluripotent Stem Cells, Fluorescent Antibody Technique, Biochemistry, Models, Biological, Transcriptome, Extracellular matrix, 03 medical and health sciences, 0302 clinical medicine, Genetics, Humans, Gene Regulatory Networks, RNA, Messenger, Induced pluripotent stem cell, Molecular Biology, Transcription factor, Cells, Cultured, 030304 developmental biology, Regulation of gene expression, 0303 health sciences, Chemistry, Wnt signaling pathway, Computational Biology, Gene Expression Regulation, Developmental, Promoter, Cell Differentiation, Cell cycle, Immunohistochemistry, Cell biology, Gene Ontology, Organ Specificity, 030220 oncology & carcinogenesis, Hepatocytes, Biomarkers, Transcription Factors
Abstract

International audience; We investigated the human induced pluripotent stem cells (hiPSCs) during a sequential in vitro step-by-step differentiation into hepatocyte-like cells (HLCs) using nanoCAGE, a method for promoters, transcription factors, and transcriptome analysis. Specific gene clusters reflected the different steps of the hepatic differentiation. The proliferation step was characterized by a typical cell cycle and DNA replication. The hepatic endoderm and the HLC steps were marked by a common signature including cell interactions with extracellular matrix (ECM), lipoproteins and hepatic biomarkers (such as albumin and alpha-fetoprotein). The specific HLC profile was characterized by important transcription factors such as HIF1A, JUN, MAF, KLF6, BMP4 and with a larger expression of genes related to Wnt signaling, extracellular matrix, lipid metabolism, urea cycle, drugs, and solute transporters. HLC profile was also characterized by the activation of upstream regulators such as HNF1A, MEIS2, NFIX, WRNIP1, SP4, TAL1. Their regulatory networks highlighted HNF4a as a bridge and linked them to important processes such as EMT–MET transitions, ECM remodeling and liver development pathways (HNF3, PPARA signaling, iron metabolism) along the different steps of differentiation.

Published
2019
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14. Investigation of the hepatic development in the coculture of hiPSCs-derived LSECs and HLCs in a fluidic microenvironment

Author

Rachid Jellali, Morgane Le Gall, Françoise Gilard, Mathieu Danoy, Bertrand Gakière, Yukio Kato, Atsushi Miyajima, Eric Leclerc, Stéphane Poulain, Hiroshi Arakawa, Johanna Bruce, Marjorie Leduc, Yannick Tauran, Yuta Koui, Taketomo Kido, Yasuyuki Sakai, Charles Plessy, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), RIKEN Center for Life Science Technologies (RIKEN CLST), RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Plateforme protéomique 3P5 [Institut Cochin] (3P5), Institut Cochin (IC UM3 (UMR 8104 / U1016)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Kanazawa University (KU), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ANR-16-RHUS-0005,iLite,iLite(2016)

Subjects
Cell type, Angiogenesis, [SDV]Life Sciences [q-bio], Cell, Biomedical Engineering, Biophysics, Bioengineering, Biomaterials, Transcriptome, 03 medical and health sciences, 0302 clinical medicine, Medical technology, medicine, R855-855.5, Induced pluripotent stem cell, 030304 developmental biology, 0303 health sciences, Chemistry, Liver cell, GATA3, Articles, In vitro, Cell biology, medicine.anatomical_structure, 030220 oncology & carcinogenesis, TP248.13-248.65, Biotechnology
Abstract

International audience; Interactions between the different liver cell types are critical to the maintenance or induction of their function in vitro. In this work, human-induced Pluripotent Stem Cells (hiPSCs)-derived Liver Sinusoidal Endothelial Cells (LSECs) and Hepatocytes-Like Cells (HLCs) were cultured and matured in a microfluidic environment. Both cell populations were differentiated in Petri dishes, detached, and inoculated in microfluidic biochips. In cocultures of both cell types, the tissue has exhibited a higher production of albumin (3.19 vs 5.31 μg/mL/106 cells in monocultures and cocultures) as well as a higher inducibility CYP450 over monocultures of HLCs. Tubular-like structures composed of LSECs and positive for the endothelial marker PECAM1, as well as a tissue more largely expressing Stabilin-2 were detected in cocultures only. In contrast, monocultures exhibited no network and less specific endothelial markers. The transcriptomic analysis did not reveal a marked difference between the profiles of both culture conditions. Nevertheless, the analysis allowed us to highlight different upstream regulators in cocultures (SP1, EBF1, and GATA3) and monocultures (PML, MECP2, and NRF1). In cocultures, the multi-omics dataset after 14 days of maturation in biochips has shown the activation of signaling related to hepatic maturation, angiogenesis, and tissue repair. In this condition, inflammatory signaling was also found to be reduced when compared to monocultures as illustrated by the activation of NFKB and by the detection of several cytokines involved in tissue injury in the latter. Finally, the extracted biological processes were discussed regarding the future development of a new generation of human in vitro hepatic models.

Published
2021
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15. EGFL7 regulates sprouting angiogenesis and endothelial integrity in a human blood vessel model

Author

Yukiko T. Matsunaga, Joris Pauty, Ryo Usuba, Fabrice Soncin, SONCIN, Fabrice, Center for International Research on Integrative Biomedical Systems [University of Tokyo] (CIBiS), Institute of Industrial Science (IIS), The University of Tokyo (UTokyo)-The University of Tokyo (UTokyo), The University of Tokyo (UTokyo), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Université de Lille, Mécanismes de tumorigenèse et thérapies ciblées, Institut Pasteur de Lille, and Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)

Subjects
Vascular Endothelial Growth Factor A, EGF Family of Proteins, Biophysics, Notch signaling pathway, Neovascularization, Physiologic, Bioengineering, 02 engineering and technology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], In vitro 3D model, Biomaterials, Adherens junction, Capillary Permeability, 03 medical and health sciences, Human Umbilical Vein Endothelial Cells, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Humans, Vascular permeability, Microvessel, Barrier function, 030304 developmental biology, Sprouting angiogenesis, 0303 health sciences, Gene knockdown, Chemistry, Calcium-Binding Proteins, Endothelial Cells, Vascular endothelial growth factor (VEGF), 021001 nanoscience & nanotechnology, Cell biology, Mechanics of Materials, Gene Knockdown Techniques, Microvessels, Ceramics and Composites, Endothelial cell junction, EGFL7, Angiogenesis, 0210 nano-technology, Epidermal growth factor-like domain 7 (EGFL7), Filopodia
Abstract

International audience; Elucidating the mechanisms underlying sprouting angiogenesis and permeability should enable the development of more effective therapies for various diseases, including retinopathy, cancer, and other vascular disorders. We focused on epidermal growth factor-like domain 7 (EGFL7) which plays an important role in NOTCH signaling and in the organization of angiogenic sprouts. We developed an EGFL7-knockdown in vitro microvessel model and investigated the effect of EGFL7 at a tissue level. We found EGFL7 knockdown suppressed VEGF-A-induced sprouting angiogenesis accompanied by an overproduction of endothelial filopodia and reduced collagen IV deposition at the basal side of endothelial cells. We also observed impaired barrier function which reflected an inflammatory condition. Furthermore, our results showed that proper formation of adherens junctions and phosphorylation of VE-cadherin was disturbed. In conclusion, by using a 3D microvessel model we identified novel roles for EGFL7 in endothelial function during sprouting angiogenesis.

Published
2019
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16. Elucidating the mechanism of the considerable mechanical stiffening of DNA induced by the couple Zn2+/Calix[4]arene-1,3-O-diphosphorous acid

Author

Dominique Collard, Laurent Jalabert, Didier Leonard, Mickael Desbrosses, Nicolas Lafitte, Yannick Tauran, Beom Joon Kim, Laurent Mollet, Jean Baptiste Gerves, Mehmet C. Tarhan, Ikjoo Byun, Florent Perret, Christelle Goutaudier, Anthony W. Coleman, Hiroyuki Fujita, Momoko Kumemura, Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Physique-IEMN (PHYSIQUE-IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo), Thermodynamique des solutions et des polymères (TSP), Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Centre National de la Recherche Scientifique (CNRS), Surfaces, Institut des Sciences Analytiques (ISA), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Physique - IEMN (PHYSIQUE - IEMN), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL)

Subjects
inorganic chemicals, Silicon, Phosphorous Acids, [SDV]Life Sciences [q-bio], chemistry.chemical_element, lcsh:Medicine, Zinc, 010402 general chemistry, 01 natural sciences, Article, Polymerization, chemistry.chemical_compound, Molecular level, lcsh:Science, Multidisciplinary, 010405 organic chemistry, Chemistry, Hydrogen bond, lcsh:R, Hydrogen Bonding, DNA, [CHIM.MATE]Chemical Sciences/Material chemistry, Phosphate, 3. Good health, 0104 chemical sciences, Stiffening, Crystallography, Zinc Compounds, lcsh:Q, Calixarenes
Abstract

International audience; The couple Calix[4]arene-1,3-O-diphosphorous acid (C4diP) and zinc ions (Zn 2+) acts as a synergistic DNA binder. Silicon NanoTweezer (SNT) measurements show an increase in the mechanical stiffness of DNA bundles by a factor of >150, at Zn 2+ to C4diP ratios above 8, as compared to Zinc alone whereas C4diP alone decreases the stiffness of DNA. Electroanalytical measurements using 3D printed devices demonstrate a progression of events in the assembly of C4diP on DNA promoted by zinc ions. A mechanism at the molecular level can be deduced in which C4diP initially coordinates to DNA by phosphate-phosphate hydrogen bonds or in the presence of Zn 2+ by Zn 2+ bridging coordination of the phosphate groups. Then, at high ratios of Zn 2+ to C4diP, interdigitated dimerization of C4diP is followed by cross coordination of DNA strands through Zn 2+ /C4diP inter-strand interaction. The sum of these interactions leads to strong stiffening of the DNA bundles and increased inter-strand binding. DNA is one of the key bio-polymers in life, encoding the information required to build organisms. Defects in DNA can occur either via natural mutations or through external damage 1. Such defects are the cause of a vast range of diseases, from Huntington's disease to cancer, although some mutations may be beneficial 2. In recent years, the rise of artificial nanostructures based on DNA often coupled to metal ions, has been pioneered by Seeman 3. Artificial DNA binders present a great interest in the biomedical field 4. They can be used in various ways, such as the transportation of DNA through the cell membrane in transfection or gene therapy 5 for blocking a specific DNA sequence to avoid the transcription of a specific gene 6 or arresting DNA replication in cancer treatment 7. The calix[n]arenes, are well known for their ability to interact with a wide range of biomolecules including amino acids, nucleotides, surfactants, proteins and DNA 8 , leading to a wide panoply of biological applications 9. Cationic calix[n]arene derivatives have been reported to be efficient DNA binders 10. However, such derivatives present several drawbacks in biology, including toxicity due to the destabilization of cellular membranes possessing negative potential or non-specific electrostatic aggregation by negatively charged DNA 11. Protein ligands of DNA (finger zinc proteins, transcription factors, nucleases) interact mainly through conjoint coordination

Published
2018
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17. Self-identification algorithm for zeolite-based thermal capacity gas sensor

Author

Hussein Awala, S. Inoue, A. Mita-Tixier, Yoshio Mita, Didier Robbes, Svetlana Mintova, Maxime Harnois, Corentin Jorel, Mathieu Pouliquen, C. Radu, O. De Sagazan, Eric Lebrasseur, Julien Grand, Yuki Okamoto, Kentaro Yamada, Matthieu Denoual, Laboratoire d'automatique de Caen (LAC), École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU), Equipe Electronique - Laboratoire GREYC - UMR6072, Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Laboratoire catalyse et spectrochimie (LCS), Normandie Université (NU)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Université de Caen Normandie (UNICAEN), Institut d'Électronique et des Technologies du numéRique (IETR), Nantes Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo), Institut d'Electronique et de Télécommunications de Rennes ( IETR ), Université de Nantes ( UN ) -Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Institut National des Sciences Appliquées - Rennes ( INSA Rennes ) -CentraleSupélec-Centre National de la Recherche Scientifique ( CNRS ), Denain Anzin Minéraux, National Institute for Plasma and Radiation Physics ( NILPRP ), Centre de recherche, Groupe de Recherche en Informatique, Image, Automatique et Instrumentation de Caen ( GREYC ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Ecole Nationale Supérieure d'Ingénieurs de Caen ( ENSICAEN ), Normandie Université ( NU ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire catalyse et spectrochimie ( LCS ), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies ( FEMTO-ST ), Université de Technologie de Belfort-Montbeliard ( UTBM ) -Ecole Nationale Supérieure de Mécanique et des Microtechniques ( ENSMM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Institute for Material Research ( IMR ), Tohoku University [Sendai], Institute for Solid State Physics, The University of Tokyo, Université de Nantes (UN)-Université de Rennes 1 (UR1), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Nantes (UN)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), and intelligente Semiconductor Microsystem Laboratory (iSML)

Subjects
Materials science, Silicon, chemistry.chemical_element, zeolites, 02 engineering and technology, engineering.material, 01 natural sciences, Heat capacity, thermal capacity gas sensor, 010309 optics, Condensed Matter::Materials Science, Coating, 0103 physical sciences, Thermal, [ SPI ] Engineering Sciences [physics], system identification algorithm, Electrical and Electronic Engineering, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Zeolite, ComputingMilieux_MISCELLANEOUS, [ PHYS ] Physics [physics], Atmospheric pressure, Extraction (chemistry), 021001 nanoscience & nanotechnology, Condensed Matter Physics, Microstructure, Electronic, Optical and Magnetic Materials, chemistry, Hardware and Architecture, engineering, 0210 nano-technology, Algorithm
Abstract

International audience; We demonstrate a new operation mode of thermal gas sensor based on thermal capacity extraction with identification algorithm. The system is a silicon microstructure covered with zeolites operated at constant temperature while stimulated by heat pseudo-random sequence. The proposed detection principle is demonstrated at room temperature and atmospheric pressure through the detection of gas water molecules with an hydrophilic FAU-type zeolite coating. The identification algorithm is a continuous-time closed-loop identification algorithm based on the instrumental variable principle.

Published
2018
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18. The solid-state structures of organic salts formed by calix[4]arene dihydroxyphosphonic acid with nucleic bases cations: adeninium, cytosinium, guaninium and uracilium

Author

Catherine Journet-Gautier, Adina N. Lazar, Florent Perret, Yannick Tauran, Beom Joon Kim, Anthony W. Coleman, Kinga Suwińska, Alda Navaza, Dominique Collard, Aleksander Shkurenko, Division of Physical Sciences and Engineering (KAUST) (KAUST), King Abdullah University of Science and Technology (KAUST), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physicochimie biomoléculaire et cellulaire (LPBC), Université Paris 13 (UP13)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Cardinal Stefan Wyszynski University in Warsaw, and Kazan Federal University (KFU)

Subjects
chemistry.chemical_classification, Base (chemistry), 010405 organic chemistry, Chemistry, [SDV]Life Sciences [q-bio], Solid-state, General Chemistry, Crystal structure, [CHIM.MATE]Chemical Sciences/Material chemistry, 010402 general chemistry, 01 natural sciences, Small molecule, 0104 chemical sciences, Crystallography, Biological property, Nucleic acid, [CHIM.CRIS]Chemical Sciences/Cristallography, Molecule, [CHIM.COOR]Chemical Sciences/Coordination chemistry, X ray analysis, ComputingMilieux_MISCELLANEOUS
Abstract

Calix[4]arene dihydroxyphosphonic acid has been demonstrated to possess an interesting range of biological properties, including atypical anti-cancer activity. The robustness of calix[4]arene dihydroxyphosphonic acid and its ubiquitous dimeric motif offers perspectives for pre-defined solid state complexation with small molecules. In the current article we describe co-crystals (organic salts) of calix[4]arene dihydroxyphosphonic acid with four nucleic base cations: adeninium, cytosinium, guaninium and uracilium. A number of characteristic interactions between the components in the four co-crystals are pointed out also using the Hirshfeld surface analysis. All the four co-crystals are based on layers of calix[4]arene dimers, alternating with layers of nucleic acid molecules. Two of the reported crystal structures (cytosinium and guaninium) are 1D channel-type structures, while the two others (adeninium and uracilium) represent 2D channel-type structures. In three out of four reported structures, interactions between the cations of nucleic bases are present generating 1D chains of cations. A constant motif is that the nucleic base is present in a type of cavity formed by one aromatic ring and a phosphonic acid moiety.

Published
2018
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19. Massively parallel and multiparameter titration of biochemical assays with droplet microfluidics

Author

Yannick Rondelez, Jean-François Bartolo, Valérie Taly, Nicolas Bredeche, Shu Okumura, Elia Henry, Teruo Fujii, Rémi Sieskind, Nathanael Aubert-Kato, Alexandre Baccouche, Anthony J. Genot, TALY, Valerie, Retour Post-Doctorant - Nano Puce digitial a ADN - - DigiNANO2013 - ANR-13-PDOC-0001 - PDOC - VALID, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Earth-Life Science Institute [Tokyo] (ELSI), Tokyo Institute of Technology [Tokyo] (TITECH), Institute of Industrial Science (IIS), The University of Tokyo (UTokyo), Gulliver (UMR 7083), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Department of Information Science [Tokyo, Japan], Ochanomizu University, Institut supérieur technologique Montplaisir (ISTM), Conception et application de molécules bioactives (CAMB), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Médecine Personnalisée, Pharmacogénomique, Optimisation Thérapeutique (MEPPOT - U1147), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Equipe labellisée Ligue contre le Cancer, Y.R. acknowledges support from the Japan Society for the Promotion of Science (JSPS) through a Grant-in-Aid for Scientific Research on Innovative Areas ‘Synthetic Biology for Comprehension of Biomolecular Networks’ (project number 23119001). A.J.G. acknowledges support from the ANR (ANR-13-PDOC-0001) and the JSPS (postdoctoral fellowship).J.-F.B. was supported by a PhD fellowship from Region Alsace. N.B. acknowledges support from the PHC Sakura program (project number 34171WG). A.B. and N.A.-K. were supported by the ELSI Origins Network (EON), which is supported by a grant from the John Templeton Foundation., ANR-13-PDOC-0001,DigiNANO,Nano Puce digitial a ADN(2013), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo, Gulliver, ESPCI ParisTech-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Equipe Labellisée par la Ligue Nationale contre le Cancer, and LNCC

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV]Life Sciences [q-bio], Microfluidics, 02 engineering and technology, Bioinformatics, Barcode, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, law.invention, Chemical kinetics, Surface-Active Agents, law, Fluorescence microscope, Image Processing, Computer-Assisted, Massively parallel, Fluorescent Dyes, Chemistry, 010401 analytical chemistry, Titrimetry, Equipment Design, Microfluidic Analytical Techniques, 021001 nanoscience & nanotechnology, Fluorescence, 0104 chemical sciences, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV] Life Sciences [q-bio], Reagent, Titration, Biological Assay, 0210 nano-technology, Biological system
Abstract

International audience; Biochemical systems in which multiple components take part in a given reaction are of increasing interest. Because the interactions between these different components are complex and difficult to predict from basic reaction kinetics, it is important to test for the effect of variations in the concentration for each reagent in a combinatorial manner. For example, in PCR, an increase in the concentration of primers initially increases template amplification, but large amounts of primers result in primer-dimer by-products that inhibit the amplification of the template. Manual titration of biochemical mixtures rapidly becomes costly and laborious, forcing scientists to settle for suboptimal concentrations. Here we present a droplet-based microfluidics platform for mapping of the concentration space of up to three reaction components followed by detection with a fluorescent readout. The concentration of each reaction component is read through its internal standard (barcode), which is fluorescent but chemically orthogonal. We describe in detail the workflow, which comprises the following: (i) production of the microfluidics chips, (ii) preparation of the biochemical mixes, (iii) their mixing and compartmentalization into water-in-oil emulsion droplets via microfluidics, (iv) incubation and imaging of the fluorescent barcode and reporter signals by fluorescence microscopy and (v) image processing and data analysis. We also provide recommendations for choosing the appropriate fluorescent markers, programming the pressure profiles and analyzing the generated data. Overall, this platform allows a researcher with a few weeks of training to acquire ∼10,000 data points (in a 1D, 2D or 3D concentration space) over the course of a day from as little as 100-1,000 μl of reaction mix.

Published
2017
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20. Zeolite-based Thermal Mass Gas Sensor with Self-Identification Algorithm

Author

Didier Robbes, Hussein Awala, Kentaro Yamada, Eric Lebrasseur, Julien Grand, C. Radu, S. Inoue, O. De Sagazan, Maxime Harnois, Yoshio Mita, M. Denoual, Corentin Jorel, Mathieu Pouliquen, Svetlana Mintova, Yuki Okamoto, A. Mita-Tixier, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Equipe Electronique - Laboratoire GREYC - UMR6072, Groupe de Recherche en Informatique, Image et Instrumentation de Caen (GREYC), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Equipe Automatique - Laboratoire GREYC - UMR6072, Laboratoire catalyse et spectrochimie (LCS), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut d'Électronique et des Technologies du numéRique (IETR), Université de Nantes (UN)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo), French and Japanese bilateral exchange program PRC JSPS-CNRSNormandy C2-MTM Project, Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU), Normandie Université (NU)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Université de Caen Normandie (UNICAEN), Nantes Université (NU)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Denoual, Matthieu, and Université de Nantes (UN)-Université de Rennes 1 (UR1)

Subjects
Materials science, Silicon, Atmospheric pressure, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Extraction (chemistry), Analytical chemistry, chemistry.chemical_element, 02 engineering and technology, engineering.material, 021001 nanoscience & nanotechnology, Microstructure, 01 natural sciences, 010309 optics, Condensed Matter::Materials Science, chemistry, Coating, 0103 physical sciences, Thermal, engineering, Thermal mass, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, 0210 nano-technology, Zeolite, Algorithm, ComputingMilieux_MISCELLANEOUS
Abstract

We demonstrate a new operation mode of thermal gas sensor based on thermal mass extraction with identification algorithm. The system is a silicon microstructure covered with zeolites operated at constant temperature while stimulated by heat pseudo-random sequence. The proposed detection principle is demonstrated at room temperature and atmospheric pressure through the detection of gas water molecules with an hydrophilic FAU-type zeolite coating.

Published
2017

21. Size and Flexibility Define the Inhibition of the H3N2 Influenza Endonuclease Enzyme by Calix[n]arenes

Author

Anthony W. Coleman, José P. Cerón-Carrasco, Horacio Pérez-Sánchez, Yannick Tauran, Moez Rhimi, Dominique Collard, Beom Joon Kim, Florent Perret, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), UCAM, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo), Institut Supérieur de l'Electronique et du Numérique - Lille (ISEN-Lille), Institut supérieur de l'électronique et du numérique (ISEN), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), The University of Tokyo-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), and The University of Tokyo

Subjects
0301 basic medicine, Microbiology (medical), h3n2 virus, Stereochemistry, viruses, enzyme inhibitors, [SDV]Life Sciences [q-bio], anti-viral activity, calix[n]arene, complex mixtures, 01 natural sciences, Biochemistry, Microbiology, Article, endonuclease, Catalysis, 03 medical and health sciences, Endonuclease, molecular docking, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Pharmacology (medical), General Pharmacology, Toxicology and Pharmaceutics, Binding site, IC50, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, biology, 010405 organic chemistry, Chemistry, lcsh:RM1-950, virus diseases, [CHIM.MATE]Chemical Sciences/Material chemistry, respiratory tract diseases, 0104 chemical sciences, 3. Good health, lcsh:Therapeutics. Pharmacology, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, 030104 developmental biology, Infectious Diseases, Enzyme, biology.protein
Abstract

Inhibition of H3N2 influenza PA endonuclease activity by a panel of anionic calix[n]arenes and &beta, cyclodextrin sulfate has been studied. The joint experimental and theoretical results reveal that the larger, more flexible and highly water-soluble sulfonato-calix[n]arenes have high inhibitory activity, with para-sulfonato-calix[8]arene, SC8, having an IC50 value of 6.4 &mu, M. Molecular docking calculations show the SC8 can interact at both the polyanion binding site and also the catalytic site of H3N2 influenza PA endonuclease.

Published
2019
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22. Anionic Calixarene-Capped Silver Nanoparticles Show Species-Dependent Binding to Serum Albumins

Author

Arnaud Brioude, Beom Joon Kim, Anthony W. Coleman, Yannick Tauran, Florent Perret, Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
Swine, Metal Nanoparticles, Pharmaceutical Science, 01 natural sciences, Silver nanoparticle, [SPI.MAT]Engineering Sciences [physics]/Materials, Analytical Chemistry, Drug Discovery, Calixarene, Bovine serum albumin, ComputingMilieux_MISCELLANEOUS, biology, Chemistry, Serum Albumin, Bovine, [CHIM.MATE]Chemical Sciences/Material chemistry, Human serum albumin, 3. Good health, Chemistry (miscellaneous), [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Molecular Medicine, Protein Binding, medicine.drug, calixarenes, Silver, Serum albumin, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, Article, Fluorescence spectroscopy, lcsh:QD241-441, species dependence, Species Specificity, lcsh:Organic chemistry, medicine, Animals, Humans, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Physical and Theoretical Chemistry, Sheep, Chromatography, Quenching (fluorescence), 010405 organic chemistry, Organic Chemistry, Albumin, 0104 chemical sciences, molecular recognition, serum albumins, biology.protein, Cattle, Nuclear chemistry
Abstract

The anionic calixarenes para-sulphonatocalix[4]arene and 1,3-di-O-phosphonatocalix[ 4]arene, have been used to cap silver nanoparticles. The binding of these functional particles with regard to various serum albumins (bovine serum albumin, human serum albumin, porcine serum albumin and sheep serum albumin) has been studied by variable temperature fluorescence spectroscopy. The quenching of the fluorescence of the proteins was shown to vary as a function of the anionic calixarene capping molecule and also as a function of the origin of the serum albumin. It is thus possible to discriminate between the different species.

Published
2013
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23. High-resolution mapping of bifurcations in nonlinear biochemical circuits

Author

Teruo Fujii, Jean-François Bartolo, Valérie Taly, R. Sieskind, Alexandre Baccouche, Nicolas Bredeche, Nathanael Aubert-Kato, Anthony J. Genot, Yannick Rondelez, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Équipe Nano Ingénierie et Intégration des Systèmes (LAAS-N2IS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées, Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Gulliver (UMR 7083), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Ochanomizu University, Earth-Life Science Institute [Tokyo] (ELSI), Tokyo Institute of Technology [Tokyo] (TITECH), Institut des Systèmes Intelligents et de Robotique (ISIR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), AMAC, Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Conception et application de molécules bioactives (CAMB), Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Médecine Personnalisée, Pharmacogénomique, Optimisation Thérapeutique (MEPPOT - U1147), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Universitaires des Saints-Pères, TALY, Valerie, Université Toulouse Capitole (UT Capitole), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse Capitole (UT Capitole), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), and Université de Toulouse (UT)

Subjects
0301 basic medicine, DNA Replication, Models, Molecular, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Bistability, Biochemical Phenomena, General Chemical Engineering, Computation, [SDV]Life Sciences [q-bio], Topology, Models, Biological, law.invention, 03 medical and health sciences, DNA computing, law, Biological Clocks, Nanotechnology, Gene Regulatory Networks, Digital electronics, Analogue electronics, Chemistry, business.industry, General Chemistry, DNA, Inverse problem, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV] Life Sciences [q-bio], Nonlinear system, 030104 developmental biology, Nonlinear Dynamics, Synthetic Biology, business, Curse of dimensionality
Abstract

International audience; Analog molecular circuits can exploit the nonlinear nature of biochemical reaction networks to compute low-precision outputs with fewer resources than digital circuits. This analog computation is similar to that employed by gene-regulation networks. Although digital systems have a tractable link between structure and function, the nonlinear and continuous nature of analog circuits yields an intricate functional landscape, which makes their design counter-intuitive, their characterization laborious and their analysis delicate. Here, using droplet-based microfluidics, we map with high resolution and dimensionality the bifurcation diagrams of two synthetic, out-of-equilibrium and nonlinear programs: a bistable DNA switch and a predator-prey DNA oscillator. The diagrams delineate where function is optimal, dynamics bifurcates and models fail. Inverse problem solving on these large-scale data sets indicates interference from enzymatic coupling. Additionally, data mining exposes the presence of rare, stochastically bursting oscillators near deterministic bifurcations.

Published
2016
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24. Synthesis and materialization of a reaction-diffusion French flag pattern

Author

Adrian Zambrano, Anton S. Zadorin, André Estevez-Torres, Yannick Rondelez, Georg Urtel, Guillaume Gines, Jean-Christophe Galas, Vadim Dilhas, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601), Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Laboratoire Kastler Brossel (LKB (Jussieu)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Jean Perrin (LJP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo-Centre National de la Recherche Scientifique (CNRS), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et de Biologie Moléculaire et Cellulaire ( IGBMC ), Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques ( LCBPT - UMR 8601 ), Université Paris Descartes - Paris 5 ( UPD5 ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratory for Integrated Micro Mechatronics Systems ( LIMMS ), The University of Tokyo-Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Kastler Brossel ( LKB (Jussieu) ), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris ( FRDPENS ), Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Paris ( ENS Paris ) -Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Paris ( ENS Paris ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Jean Perrin ( LJP ), and Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS )

Subjects
0301 basic medicine, Bistability, General Chemical Engineering, Pattern formation, FOS: Physical sciences, Nanotechnology, Pattern Formation and Solitons (nlin.PS), Synthetic analogue, 03 medical and health sciences, Reaction–diffusion system, Physics - Biological Physics, Chemistry, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, Soft materials, Nonlinear Sciences - Pattern Formation and Solitons, Nonlinear Sciences - Adaptation and Self-Organizing Systems, [CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry, 030104 developmental biology, Biological Physics (physics.bio-ph), [ CHIM.MATE ] Chemical Sciences/Material chemistry, [ CHIM.THEO ] Chemical Sciences/Theoretical and/or physical chemistry, [ PHYS.COND.CM-SCM ] Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft], Biological system, Protein concentration, [PHYS.COND.CM-SCM]Physics [physics]/Condensed Matter [cond-mat]/Soft Condensed Matter [cond-mat.soft], Adaptation and Self-Organizing Systems (nlin.AO), Morphogen, Flag (geometry)
Abstract

During embryo development, patterns of protein concentration appear in response to morphogen gradients. These patterns provide spatial and chemical information that directs the fate of the underlying cells. Here, we emulate this process within non-living matter and demonstrate the autonomous structuration of a synthetic material. Firstly, we use DNA-based reaction networks to synthesize a French flag, an archetypal pattern composed of three chemically-distinct zones with sharp borders whose synthetic analogue has remained elusive. A bistable network within a shallow concentration gradient creates an immobile, sharp and long-lasting concentration front through a reaction-diffusion mechanism. The combination of two bistable circuits generates a French flag pattern whose 'phenotype' can be reprogrammed by network mutation. Secondly, these concentration patterns control the macroscopic organization of DNA-decorated particles, inducing a French flag pattern of colloidal aggregation. This experimental framework could be used to test reaction-diffusion models and fabricate soft materials following an autonomous developmental program., Comment: Accepted version after embargo. Fig 4 now show materialization of a French flag pattern. Supplementary materials have been substantially improved

Published
2016
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25. Bio-Applications of Calix[n]arene Capped Silver Nanoparticles

Author

Anthony W. Coleman, Beom Joon Kim, Yannick Tauran, Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Center for International Research on MicroMechatronics (CIRMM), and The University of Tokyo (UTokyo)-IIS, Institute of Industrial Sciences

Subjects
Materials science, Silver, [SDV]Life Sciences [q-bio], Biomedical Engineering, Nanoparticle, Metal Nanoparticles, Bioengineering, Nanotechnology, Biocompatible Materials, Silver nanoparticle, Calixarene, Molecule, General Materials Science, Antiviral, Metal nanoparticles, Therapeutically Agents, chemistry.chemical_classification, Biomolecules, Biomolecule, Optical Biosensors, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, Condensed Matter Physics, Biocompatible material, Antibacterial, chemistry, Calixarenes, Reactive Oxygen Species, Silver Nanoparticles, Biosensor, Calix[n]arenes
Abstract

International audience; During the 10 last years, there has been a growing interest in calix[n]arene capped silver nanopar-ticles for their uses in biosensing and much more recently for their intrinsic therapeutic properties. Cost effective, portable and ultra-sensitive analytical tools are one of the major expectations of silver nanoparticles capped with calix[n]arenes. Their uses for detecting a wide range of hazardous molecules and biological compounds by different physical approaches (optical or electrical) are reviewed in depth here. A second part deals with their biological activities. These hybrid nanopar-ticles have been shown to possess antibacterial properties, and to reach antiviral and anti-cancer targets.

Published
2015
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26. Large negatively charged organic host molecules as inhibitors of endonuclease enzymes

Author

Yannick Tauran, Anthony W. Coleman, Beom Joon Kim, Christophe Anjard, Moez Rhimi, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo, Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, UMI 2820, Inst Ind Sci, CIRMM, Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Universite of Lyon 1, LIMMS, Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo), Études des Structures, des Processus d’Adaptation et des Changements de l’Espace (ESPACE), Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Avignon Université (AU)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Center for Internationnal Research on Micronano Mechatronics (CIRMM), and Y.T. thanks the Universite of Lyon 1 and LIMMS for financial aid.

Subjects
Stereochemistry, organic, [SDV]Life Sciences [q-bio], 010402 general chemistry, 01 natural sciences, Catalysis, 03 medical and health sciences, Endonuclease, Materials Chemistry, Deoxyribonuclease I, Humans, Molecule, molecules, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Enzyme Inhibitors, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Range (particle radiation), Binding Sites, biology, Chemistry, Host (biology), beta-Cyclodextrins, Metals and Alloys, DNA, General Chemistry, Endonucleases, 0104 chemical sciences, 3. Good health, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Enzyme, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Ceramics and Composites, biology.protein, Nanoparticles, Calixarenes
Abstract

International audience; Three large negatively charged organic host molecules; β-cyclodextrin sulphate, para-sulphonato-calix[6]arene and para-sulphonato-calix[8]arene have been shown to be effective inhibitors of endonuclease in the low micromolar range, additionally para-sulphonato-calix[8]arene is a partial inhibitor of rhDNase I.

Published
2014
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27. Molecular Recognition and Transport of Active Pharmaceutical Ingredients on Anionic Calix[4]arene-Capped Silver Nanoparticles

Author

Kinga Suwinska, Florent Perret, Maxime Boulet, Yannick Tauran, Anthony W. Coleman, Beom Joon Kim, Cyrielle Chassain-Nely, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Multimatériaux et Interfaces (LMI), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Cardinal Stefan Wyszynski University in Warsaw, Polish Academy of Sciences (PAN), Center for International Research on MicroMechatronics (CIRMM), and The University of Tokyo (UTokyo)-IIS, Institute of Industrial Sciences

Subjects
Article Subject, [SDV]Life Sciences [q-bio], [CHIM.THER]Chemical Sciences/Medicinal Chemistry, 010402 general chemistry, 01 natural sciences, Silver nanoparticle, lcsh:Chemistry, chemistry.chemical_compound, Molecular recognition, Polymer chemistry, medicine, Organic chemistry, [CHIM.COOR]Chemical Sciences/Coordination chemistry, Carboxylate, Surface plasmon resonance, ComputingMilieux_MISCELLANEOUS, Active ingredient, 010405 organic chemistry, Chemistry, Chloramphenicol, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, Phosphonate, 0104 chemical sciences, Gentamicin Sulfate, lcsh:QD1-999, medicine.drug
Abstract

A series of six anionic calix[4]arenes, having sulphonate, carboxylate, or phosphonate functions at either the para-aromatic position or the phenolic face were used to cap silver nanoparticles. Their molecular recognition properties were studied with regard to three active pharmaceutical ingredients, chlorhexidine, chloramphenicol, and. gentamycin sulfate. Of these APIs chlorhexidine is known to form cocrystals with the anionic calix[4]arenes, gentamicin sulfate is an aminoglycosidic antibiotic, and chloramphenicol is a neutral antibiotic. As expected the former two APIs show clear complexation behavior as demonstrated by shifts in the visible spectra whereas the last shows no modification in the wavelength of the plasmon resonance of the silver nanoparticles.

Published
2013
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28. Discriminatory antibacterial effects of calix[n]arene capped silver nanoparticles with regard to Gram positive and Gram negative bacteria

Author

Anthony W. Coleman, Héla Mkaouar, Florent Perret, Samira Boudebbouze, Moez Rhimi, Beom Joon Kim, Arnaud Brioude, Emmanuelle Maguin, Alexandrine Garnier, Yannick Tauran, Microbiota Interaction with Human and Animal (MIHA), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Institut National de la Recherche Agronomique (INRA)-AgroParisTech, AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire de Chimie - UMR5182 (LC), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Institut de Chimie du CNRS (INC), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Silver, Gram-negative bacteria, Stereochemistry, Gram-positive bacteria, [SDV]Life Sciences [q-bio], Metal Nanoparticles, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, Gram-Positive Bacteria, 010402 general chemistry, 01 natural sciences, Medicinal chemistry, Catalysis, Silver nanoparticle, [SPI.MAT]Engineering Sciences [physics]/Materials, Gram-Negative Bacteria, Materials Chemistry, Molecule, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Alkyl, ComputingMilieux_MISCELLANEOUS, Gram, chemistry.chemical_classification, Molecular Structure, biology, 010405 organic chemistry, Metals and Alloys, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, biology.organism_classification, ACIDS, Anti-Bacterial Agents, 3. Good health, 0104 chemical sciences, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, chemistry, Ceramics and Composites, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Calixarenes, NOBLE-METAL NANOPARTICLES
Abstract

International audience; Silver nanoparticles capped with nine different sulphonated calix[n] arenes were tested for their anti-bacterial effects against B. subtilis and E. coli at an apparent concentration of 100 nM in calix[n] arene. The results show the para-sulphonato-calix[n] arenes are active against Gram positive bacteria and the derivatives having sulphonate groups at both para and alkyl terminal positions are active against Gram negative bacteria. The calix[6] arene derivative with only O-alkyl sulphonate groups shows bactericidal activity.

Published
2013
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29. Calix-arene silver nanoparticles interactions with surfactants are charge, size and critical micellar concentration dependent

Author

Arnaud Brioude, Alessandro Cumbo, Yannick Tauran, Florent Perret, Patrick Shahgaldian, Anthony W. Coleman, Beom Joon Kim, Imed Montasser, Laboratoire des Multimatériaux et Interfaces (LMI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), School of Life Sciences [Muttenz], University of Applied Sciences Northwestern Switzerland (FHNW), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie - UMR5182 (LC), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Institut de Chimie du CNRS (INC), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL)

Subjects
Inorganic chemistry, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, 010402 general chemistry, 01 natural sciences, Micelle, Catalysis, Silver nanoparticle, [SPI.MAT]Engineering Sciences [physics]/Materials, Polymer chemistry, Materials Chemistry, Molecule, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, ComputingMilieux_MISCELLANEOUS, 010405 organic chemistry, Chemistry, Metals and Alloys, Cationic polymerization, General Chemistry, [CHIM.MATE]Chemical Sciences/Material chemistry, 0104 chemical sciences, 3. Good health, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Critical micelle concentration, Ceramics and Composites, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Absorption (chemistry)
Abstract

The interactions of silver nanoparticles capped by various calix[n]arenes bearing sulphonate groups at the para and/or phenolic faces with cationic, neutral and anionic surfactants have been studied. Changes in the plasmonic absorption show that only the calix[4]arene derivatives sulphonated at the para-position interact and then only with cationic surfactants. The interactions follow the CMC values of the surfactants either as simple molecules or mixed micelles.

Published
2012
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30. Atomistic simulation of plasticity in silicon nanowires

Author

Hiroyuki Fujita, Dominique Collard, Fabrizio Cleri, Tadashi Ishida, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), The University of Tokyo (UTokyo), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), and The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS)

Subjects
010302 applied physics, Materials science, Physics and Astronomy (miscellaneous), Silicon, Nanowire, chemistry.chemical_element, 02 engineering and technology, Plasticity, 021001 nanoscience & nanotechnology, Microstructure, 01 natural sciences, Amorphous solid, [PHYS.MECA.MEMA]Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph], Crystallography, chemistry, 0103 physical sciences, Crystallite, Deformation (engineering), Composite material, 0210 nano-technology, Necking
Abstract

We study the tensile deformation of polycrystalline Si nanowires by means of molecular dynamics simulations. The initial microstructure is composed by a network of nanocrystals glued together by a thin layer of amorphous material. Atomistic simulations could clearly identify liquidlike flow in the constrained amorphous Si as the responsible for the observed elongation. After this first stage of nearly constant-stress flow, a necking instability sets in, eventually leading to fracture, at the point when the nanowire diameter becomes comparable to the size of the nanocrystals.

Published
2010
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31. SURFACE-TEMPERATURE CONTROL OF SILICON NANOWIRES IN DRY AND LIQUID CONDITIONS

Author

Sho Akiyama, Jacques Fattaccioli, Peter Löw, Nobuyuki Takama, Y. T. Cheng, Christian Bergaud, Beom Joon Kim, Institute of Industrial Science (IIS), The University of Tokyo (UTokyo), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)

Subjects
Temperature control, Materials science, Silicon, Calibration curve, Nanowire, Analytical chemistry, chemistry.chemical_element, Temperature measurement, Rhodamine, chemistry.chemical_compound, chemistry, Rhodamine B, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Joule heating
Abstract

In this paper we present the results of surface temperature control of silicon nanowires by using fluorescent thermometry at the nanometer scale. Rhodamine B is one of the stable fluorescent molecules, which rely on the characteristic of temperature-dependent change in fluorescent intensity, and it was used for nano-scale surface temperature sensing interface. The resistive heating on Si nanowires was carried out with applying voltage potential of 6 ~ 12 V. Surface-temperature measurement was performed by converting the changes in fluorescent intensity with calibration curve of Rhodamine B. The temperature at the central line along nanowires increasing from 30 degrees to 35~70 degrees was observed.

Published
2009
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32. Lipid Bilayer Microarray for Parallel Recording of Transmembrane Ion Currents

Author

Hiroyuki Noji, Hiroaki Suzuki, Shoji Takeuchi, Bruno Le Pioufle, Kazuhito V. Tabata, Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institute of Industrial Sciences (IIS), The University of Tokyo (UTokyo), and Osaka University [Osaka]

Subjects
Optics and Photonics, Polymers, Lipid Bilayers, Analytical chemistry, 02 engineering and technology, Xylenes, 010402 general chemistry, 01 natural sciences, Ion Channels, Analytical Chemistry, Ion, chemistry.chemical_compound, Parylene, Alamethicin, Lipid bilayer, Electrodes, Electric Conductivity, Gramicidin, Membranes, Artificial, Ion current, Microarray Analysis, 021001 nanoscience & nanotechnology, Transmembrane protein, 0104 chemical sciences, Membrane, chemistry, Microscopy, Electron, Scanning, Biophysics, 0210 nano-technology, Ion Channel Gating
Abstract

International audience; This paper describes a multiwell biochip for simultaneous parallel recording of ion current through transmembrane pores reconstituted in planar lipid bilayer arrays. Use of a thin poly(p-xylylene) (parylene) film having micrometer-sized apertures (φ = 15−50 μm, t = 20 μm) led to formation of highly stable bilayer lipid membranes (BLMs) for incorporation of transmembrane pores; thus, a large number of BLMs could be arrayed without any skillful technique. We optically confirmed the simultaneous formation of BLMs in a 5 × 5 matrix, and in our durability test, the BLM lasted more than 15 h. Simultaneous parallel recording of alamethicin and gramicidin transmembrane pores in multiple contiguous recording sites demonstrated the feasibility of high-throughput screening of transmembrane ion currents in artificial lipid bilayers.

Published
2008
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33. Microfluidic Devices for Ultra Wide Band Electromagnetic Spectroscopy of Single Cell

Author

E. Lennon, Vincent Senez, Takatoki Yamamoto, Yasuyuki Sakai, A. Treizebre, Nour-Eddine Bourzgui, Bertrand Bocquet, Tahsin Akalin, Teruo Fujii, Steve Arscott, V. Mille, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Electrical Physics Department, Central Research Institute of Electric Power Industry, Institute of Industrial Science, The University of Tokyo (UTokyo), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), and The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Silicon, Microfluidics, chemistry.chemical_element, 02 engineering and technology, Low frequency, 01 natural sciences, 7. Clean energy, Modeling and simulation, [SPI]Engineering Sciences [physics], Planar, Labeling, Transmission line, Frequency measurement, 0103 physical sciences, Electronic engineering, Dielectric substrates, Spectroscopy, Electrodes, ComputingMilieux_MISCELLANEOUS, 010302 applied physics, business.industry, Electromagnetic devices, Performance analysis, 021001 nanoscience & nanotechnology, Dielectric spectroscopy, chemistry, Ultra wideband technology, Optoelectronics, 0210 nano-technology, business, Electrochemical impedance spectroscopy, Hafnium
Abstract

International audience; By performing dielectric spectroscopy across a wide spectrum of frequencies (DC-THz), single-cell analysis could be performed without labeling process. That is why we are developing different micro structures, either planar/3D electrodes or transmission lines, and microfluidic devices using BIOMEMS technologies. In our devices, silicon or glass substrates are combined with polymeric materials and gold patterns. For low frequency (LF) applications, PDMS has been chosen while for high frequency (HF) structures, the PPTMDSO is preferred. We are designing the devices thanks to modeling and simulation using either COMSOL (LF) or CST (HF) softwares. Different designs have already been fabricated and are presented in this paper. Finally, we have performed single cell measurements for the low frequency range (

Published
2006
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34. Study of osteoblastic cells in a microfluidic environment

Author

Eric Leclerc, Pierre Layrolle, B. David, Teruo Fujii, Laurent Griscom, Bruno LePioufle, C. Legallaisa, Biomécanique et génie biomédical (BIM), Centre National de la Recherche Scientifique (CNRS), Laboratoire d'ingénierie osteo-articulaire et dentaire (LIOAD), Université de Nantes (UN)-IFR26-Institut National de la Santé et de la Recherche Médicale (INSERM), Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS), Bio-MIcroSystèmes et BioSensors (SATIE-BIOMIS), Systèmes d'Information et d'Analyse Multi-Echelles (SIAME), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Centre National de la Recherche Scientifique (CNRS)-Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Physiopathologie des Adaptations Nutritionnelles (PhAN), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre de Recherche sur les Matériaux d'Intérêt Biologique, Université de Nantes (UN)-UPRES EA 2159, Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), Le Pioufle, Bruno, Université Paris-Seine-Université Paris-Seine-Conservatoire National des Arts et Métiers [CNAM] (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS), Systèmes d'Information et d'Analyse Multi-Echelles (SATIE-SIAME), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Cachan (ENS Cachan)-Université Paris-Sud - Paris 11 (UP11)-Institut Français des Sciences et Technologies des Transports, de l'Aménagement et des Réseaux (IFSTTAR)-École normale supérieure - Rennes (ENS Rennes)-Université de Cergy Pontoise (UCP), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS)-Systèmes et Applications des Technologies de l'Information et de l'Energie (SATIE), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Nantes (UN), and David, Bertrand

Subjects
MESH: 3T3 Cells, [SDV]Life Sciences [q-bio], Microfluidics, Cell Culture Techniques, MESH: Equipment Failure Analysis, 02 engineering and technology, Mechanotransduction, Cellular, MESH: Tissue Engineering, MESH: Microfluidics, Mice, chemistry.chemical_compound, MESH: Bioreactors, Bioreactors, Bone cell, MESH: Animals, Mechanotransduction, ComputingMilieux_MISCELLANEOUS, MESH: Cell Size, 0303 health sciences, Osteoblast, 3T3 Cells, Equipment Design, 021001 nanoscience & nanotechnology, [SDV] Life Sciences [q-bio], medicine.anatomical_structure, MESH: Cell Survival, Mechanics of Materials, 0210 nano-technology, Materials science, Cell Survival, [SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Biophysics, Bioengineering, 3T3 cells, Biomaterials, 03 medical and health sciences, MESH: Cell Proliferation, medicine, Animals, Viability assay, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, [SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials, MESH: Mice, Cell Proliferation, Cell Size, 030304 developmental biology, MESH: Osteoblasts, MESH: Cell Culture Techniques, Osteoblasts, Tissue Engineering, Polydimethylsiloxane, MESH: Mechanotransduction, Cellular, In vitro, [SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials, Equipment Failure Analysis, chemistry, Ceramics and Composites, Ex vivo, Biomedical engineering, MESH: Equipment Design
Abstract

International audience; Bone tissue engineering consists of culturing osteoblastic cells onto synthetic three-dimensional (3D) porous scaffolds. The organization of bone cells into 3D scaffolds is crucial for ex vivo tissue formation. Diffusional rates of nutrients could be greatly improved by perfusing media through the 3D microporous scaffolds. However, bone cells cultured in vitro are responsive to a variety of different mechanical signals including fluid flow and shear stresses. In this work, we attempt to study osteoblastic cells behaviour cultured within microdevices allowing continuous and homogenous feeding of cells. We have fabricated polydimethylsiloxane PDMS microdevices with a 3D microstructured channel network. Mouse calvarial osteoblastic cells MC3T3-E1 were seeded at 2x10(6)cells/ml and cultured into the microdevices under flow rates of 0, 5, 35 microl/min. Cells attached and proliferated well in the designed microdevices. Cell viability was found around 85% up to 1 to 2 weeks for shear stress value under 5 mPa. The alkaline phosphatase (ALP) activity was enhanced 3- and 7.5-fold inside the microdevices under static and dynamic flow of 5 microl/min as compared to flat static cultures in PDMS coated Petri dishes. Therefore, osteoblastic cells could be successfully cultured inside the microdevices under dynamic conditions and their ALP activity was enhanced. These results are promising for bone cell growth and differentiation as well as future tissue regeneration using larger 3D microfluidic microdevices.

Published
2006
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35. Determination of the d31 piezoelectric coefficient of PbZrxTi1−xO3 thin films using multilayer buckled micromembranes

Author

Liviu Nicu, Caroline Soyer, Cédric Ayela, Eric Cattan, Christian Bergaud, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

Subjects
010302 applied physics, Materials science, Piezoelectric coefficient, Silicon, General Physics and Astronomy, chemistry.chemical_element, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, Piezoelectricity, Interferometry, chemistry, Buckling, Electrical resistivity and conductivity, 0103 physical sciences, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Thin film, Composite material, 0210 nano-technology, ComputingMilieux_MISCELLANEOUS, Microscale chemistry
Abstract

International audience; The aim of this paper consists in the determination of the piezoelectric transverse coefficient d31 of PbZrxTi1-xO3 (PZT) thin films integrated in dedicated multilayer silicon-based micromembranes exhibiting an initial buckled profile. An analytical model specific to this configuration was built and used for the calculation of d31 starting with the static profiles of the microfabricated devices determined by means of a double-beam interferometer. The influence of dc voltage and buckling effects on the d31 piezoelectric coefficient at the microscale were investigated, and high values were obtained, from 30to75pm/V, within a hysteresyslike cycle. These results demonstrated the good electrical behavior of PZT thin films at the microscale with a low influence of buckling effects and determined optimal operation conditions for high values of d31.

Published
2006
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36. Microscopic agents programmed by DNA circuits

Author

Anton S. Zadorin, André Estevez-Torres, Yannick Rondelez, Jean-Christophe Galas, Teruo Fujii, Guillaume Gines, Laboratory for Integrated Micro Mechatronics Systems (LIMMS), The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS), Gulliver (UMR 7083), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Jean Perrin (LJP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), European Project: 647275,H2020,ERC-2014-CoG,ProFF(2015), Centre National de la Recherche Scientifique (CNRS)-The University of Tokyo (UTokyo), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)

Subjects
0301 basic medicine, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], Computer science, Biomedical Engineering, Bioengineering, Nanotechnology, DNA, Condensed Matter Physics, Atomic and Molecular Physics, and Optics, Chemical Dynamics, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, Models, Chemical, Distributed decision, Nucleic acid, General Materials Science, Electrical and Electronic Engineering, [SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics, Biological system, [NLIN.NLIN-AO]Nonlinear Sciences [physics]/Adaptation and Self-Organizing Systems [nlin.AO], Electronic circuit, Stable state
Abstract

International audience; Information stored in synthetic nucleic acids sequences can be used in vitro to create complex reaction networks with precisely programmed chemical dynamics. Here, we scale up this approach to program networks of microscopic particles (agents) dispersed in an enzymatic solution. Agents may possess multiple stable states, thus maintaining a memory and communicate by emitting various orthogonal chemical signals, while also sensing the behaviour of neighbouring agents. Using this approach, we can produce collective behaviours involving thousands of agents, for example retrieving information over long distances or creating spatial patterns. Our systems recapitulate some fundamental mechanisms of distributed decision making and morphogenesis among living organisms and could find applications in cases where many individual clues need to be combined to reach a decision, for example in molecular diagnostics.

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37. Silicon nanotweezers with a microfluidic cavity for the real time characterization of DNA damage under therapeutic radiation beams

Author

Gregoire Perret, Eric Lartigau, Fabrizio Cleri, Hiroyuki Fujita, Nicolas Lafitte, Momoko Kumemura, Laurent Jalabert, Hervé Guillou, Po-Tsun Chiang, Thomas Lacornerie, Teruo Fujii, Dominique Collard, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Laboratory for Integrated Micro Mechatronics Systems (LIMMS), and The University of Tokyo (UTokyo)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Silicon, Materials science, DNA damage, Microfluidics, chemistry.chemical_element, Nanotechnology, 02 engineering and technology, Radiation, Ionizing radiation, 03 medical and health sciences, Micromanipulation, 030304 developmental biology, [PHYS]Physics [physics], 0303 health sciences, X-Rays, technology, industry, and agriculture, DNA, Micro-Electrical-Mechanical Systems, Microfluidic Analytical Techniques, 021001 nanoscience & nanotechnology, Radiation pressure, chemistry, Bundle, 0210 nano-technology, Beam (structure), DNA Damage
Abstract

We report the biomechanical characterization of λ-DNA bundle exposed to a therapeutic radiation beam by silicon Nanotweezers. The micromechanical device endures the harsh environment of radiation beams, and still retains molecular-level detection accuracy. The real-time DNA bundle degradation is observed in terms of biomechanical stiffness and viscosity reduction, both in air and in solution. These results pave the way for both fundamental and clinical studies of DNA degradation mechanisms under ionizing radiation for improved tumor treatment.

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