3 results on '"Verma, Sandeep"'
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2. Enhanced growth and cardenolides production in Digitalis purpurea under the influence of different LED exposures in the plant factory
- Author
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Byoung Ryong Jeong, Sandeep Verma, Seung Jae Hwang, Saikat Gantait, BAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü, and Verma, Sandeep Kumar
- Subjects
0106 biological sciences ,0301 basic medicine ,Stomatal conductance ,Light ,Plant Development ,lcsh:Medicine ,Photosynthesis ,Growth and Cardenolides Production ,01 natural sciences ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Biomass ,lcsh:Science ,Digitalis ,Multidisciplinary ,biology ,Chemistry ,Digitalis Purpurea ,lcsh:R ,RuBisCO ,Digitalis purpurea ,Plant factory ,Agriculture ,Enhanced growth ,Environment, Controlled ,biology.organism_classification ,Trichome ,LED Exposures ,Cardenolides ,Horticulture ,030104 developmental biology ,Anthocyanin ,biology.protein ,lcsh:Q ,010606 plant biology & botany - Abstract
In this report, we have investigated the influence of different light qualities on Digitalis purpurea under a controlled environment. For this purpose, red (R), blue (B), fluorescent lamp (FL, control), along with combined red and blue (R:B) LEDs were used. Interestingly, the plant growth parameters such as number of leaf, longest root, width of leaf, width of stomata, width of trichome, leaf area, leaf or root fresh weight (FW), weight (DW) as well as length of trichome were maximum under R:B (8:2), and significantly larger than control plants. The stomatal conductance or anthocyanin was maximum under B LED than those under FL, however the photosynthesis rate was greater under FL. RuBisCO activity was maximum under R:B (1:1) LEDs while the quantity of the UV absorbing substances was highest under R LED than under FL. The maximum amount of cardenolides were obtained from leaf tissue under R:B (2:8) LED than those under FL. The R:B LEDs light was suitable for Digitalis plant growth, development, micro- and macro-elements, as well as cardenolides accumulation in the plant factory system. The adaptation of the growth strategy developed in this study would be useful for the production of optimized secondary metabolites in Digitalis spp.
- Published
- 2018
3. Endemik tıbbi tür Digitalis Lamarckii Ivan.’ın kotiledon yaprağı segmentlerinden indirekt somatik embriyo ve sürgün oluşumu
- Author
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Sandeep Verma, Ekrem Gürel, Bahtiyar Buhara Yücesan, Songül Gürel, BAİBÜ, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Verma, Sandeep Kumar, Yücesan, Bahtiyar Buhara, and Gürel, Ekrem
- Subjects
Somatic embryogenesis ,Physiology ,Organogenesis ,Biology ,Bodur Yüksükotu ,Microbiology ,Kallus Oluşumu ,Kotiledon Yaprak Segmentleri ,chemistry.chemical_compound ,Murashige and Skoog medium ,Shoot Organogenesis ,Callus Formation ,Dwarf Foxglove ,Botany ,Genetics ,Cardenolide ,Sürgün Organogenesisi ,Molecular Biology ,Cotyledonary Leaf Segments ,Somatik Embriyogenesis ,Embryo ,Cell Biology ,Somatic Embryogenesis ,Key words: Digitalis lamarckii Ivan.,dwarf foxglove,cotyledonary leaf segments,callus formation,somatic embryogenesis,shoot organogenesis ,chemistry ,Callus ,Digitalis Lamarckii Ivan ,Shoot ,General Agricultural and Biological Sciences ,Explant culture - Abstract
This study describes, for the first time, an in vitro protocol for the development of somatic embryos and shoots from a callus derived from cotyledonary leaf segments excised from 3-week-old in vitro-germinated seedlings of Digitalis lamarckii Ivan. (dwarf foxglove), an endemic medicinal species of Turkey. The embryogenic callus was induced on Murashige and Skoog (MS) medium containing 0.54 μM α-naphthalene acetic acid (NAA) and 2.22 μM 6-benzylaminopurine (BAP). Somatic embryos developed readily when the embryogenic callus was transferred to MS medium containing BAP (4.44 or 8.87 μM) alone or BAP combined with NAA (1.34, 2.69, or 5.37 μM). The most effective hormonal combination for somatic embryogenesis was 1.34 μM NAA and 8.87 μM BAP, which produced a mean of 37.0 embryos per cotyledonary leaf explant. An organogenic callus was induced on MS medium containing 2.69 μM NAA and 2.22 μM BAP. Shoot development was observed when the organogenic callus was transferred to MS medium containing different concentrations of BAP alone (2.22, 4.44, or 8.87 μM) or BAP combined with NAA (1.34 or 2.69 μM). The highest mean number of shoots (5.67 shoots per explant) was obtained when the medium contained 8.87 μM BAP and 2.69 μM NAA. The regenerated shoots were readily rooted on MS medium containing 1.0, 2.5, or 5 μM indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA). Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. Over 90% of the regenerants survived the hardening process. The protocol described could be useful for germplasm conservation, commercial cultivation, genetic improvement, and cardenolide production studies in D. lamarckii. Türkiye’nin endemik tıbbi türlerinden biri olan Digitalis lamarckii Ivan. (bodur yüksükotu)’nın in vitro koşullarda çimlendirilmiş üç haftalık fidelerinden izole edilen kotiledon yaprağı segmentlerinden elde edilen kallustan, in vitro somatik embriyo ve sürgün oluşumu protokolu ilk defa bu çalışmada tanımlanmıştır. Embriyonik kallus, 0,54 μM α-naftalen asetik asit (NAA) ile 2,22 μM 6-benzilaminopürin (BAP) içeren Murashige ve Skoog (MS) ortamında elde edilmiş, ve BAP’yi (4,44 veya 8,87 μM) tek başına veya NAA (1,34, 2,69 veya 5,37 μM) ile kombinasyon halinde içeren MS ortamında kültüre alındığında, somatik embriyolar kolaylıkla gelişmişlerdir. Embriyo rejenerasyonu için en etkili hormonal kombinasyon, 1,34 μM NAA ile 8,87 μM BAP olmuş ve kotiledon yaprak eksplantı başına ortalama 37,0 embriyo elde edilmiştir. Organogenik kallus, 2,69 μM NAA ve 2,22 μM BAP içeren MS ortamında elde edilmiş, ve BAP’yi (2,22, 4,44 veya 8,87 μM) tek başına veya NAA (1,34 veya 2,69 μM) ile kombinasyon halinde içeren MS ortamında kültüre alındığında, sürgün oluşumu gözlenmiştir. En yüksek ortalama sürgün verimi (eksplant başına 5,67 sürgün), 8,87 μM BAP ve 2,69 μM NAA içeren ortamda elde edilmiştir. Sürgünler, indol-3-asetik asit (IAA) veya NAA (1,0, 2,5 veya 5,0 μM) içeren MS ortamında kolaylıkla köklendirilmiştir. Rejenere edilen bitkiler, saksılara aktarılarak, büyümeye ve olgunlaşmaya bırakılmışlardır. Rejenere edilen bitkilerin tamamına yakını, dış koşullara alıştırma sürecini, canlılıklarını koruyarak tamamlamışlardır. Tanımlanan bu protocol, D. lamarckii’nin germplasm depolanması, ticari üretim, genetik iyileştirme ve kardenolit üretimi çalışmalarına katkı sağlayabilecektir.
- Published
- 2011
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