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β-cyclodextrin (β-CD) immobilization of a chitosan derivate on C6-OH (CTS-6-CD) with good solubility was prepared through a novel method. During the process the amino group of the chitosan was protected by phthalic anhydride and deprotected in the hydrazine hydrate solution. β-CD was immobilized onto the C6 position of chitosan by a nucleophilic displacement reaction. The prepared derivate can be dissolved in hydrazine hydrate (v:v = 1:1), ethylenediamine/ethanol (v:v = 1:1), 1% aqueous acetic acid, DMSO and DMAc. The application of the 6-OH cyclodextrin-immobilized chitosan derivatives in an electrochemical biosensor was examined in a preliminarily manner.

The reaction of CuBr2 with 1,10-phen-H2O (1,10-phen = 1,10-phenanthroline) gave two compounds: CuBr2(C12H8N2) and Cu3Br3(C12H8N2)2. Their structures have been characterized by single-crystal X-ray diffraction analysis, elemental analyses, thermogravimetric analyses (TGA) and measurement of variable temperature magnetic susceptibility. Crystal data for CuBr2(C12-H8N2): monoclinic, space group P21/n, a = 0.9977(3) nm, b = 0.65138(14) nm, c = 1.8207(4) nm, β = 91.624(18)°, V = 1.1828(5) nm3, Z = 2. Crystal data for Cu3Br3(C12H8N2)2: monoclinic, space group C2/c, a = 1.00167(11) nm, b = 1.4523(4) nm, c = 1.6295(3) nm, β = 94.386(14)°, V = 2.3635(8) nm3, Z = 3.

Biosensor detecting techniques have attracted much attention in the content determination of H2O2, which has been used illegally as a food additive. An electrochemical biosensing membrane for the detection of H2O2 was developed with C6-OH of chitosan immobilized cyclodextrin derivates (6-CD–CTS), which possessed a high cyclodextrin loading capacity (2.12 × 10−4 mol/g), as the carrier. The biosensor was prepared through the inclusion of ferrocene as the electron mediator in a hydrophobic cavity of cyclodextrin and crosslinking catalase (CAT) to 2-NH2 of 6-CD–CTS. The ferrocene-included complex was evaluated by ultraviolet–visible spectrophotometry and thermogravimetric analysis. Its electrochemical behavior was also studied. The impact of the reaction conditions on the CAT immobilization capacity was evaluated. When previous membrane was used to detect the concentration of H2O2 (CH2O2), we found that the catalysis of CAT and the signal amplification of ferrocene had a major impact on the cyclic voltammograms. The optimal working pH of the modified electrode was 7.0. The peak current (I) had a linear relationship with the H2O2 concentration (CH2O2) in the range 1.0 × 10−4 to 1.0 × 10−3 mol/L. The linear regression equation was I = 0.00475CH2O2 − 0.03025. The detection limit was 10−6 mol/L. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41499.