1. Mechanism by which Eucommia ulmoides leaves Regulate Nonalcoholic fatty liver disease based on system pharmacology
- Author
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Zhong-Yuan Guo, Ping Wang, Bingdi Cui, Zhi-Min Wang, Shaojia Liang, Lian-He Yang, Chengfu Su, Man Gong, Mengzhe Fan, Li-Ping Dai, and Xiao-Qian Liu
- Subjects
ved/biology.organism_classification_rank.species ,Blood lipids ,Eucommia ulmoides ,Pharmacology ,Network Pharmacology ,Chloroquine ,Non-alcoholic Fatty Liver Disease ,Diabetes mellitus ,Drug Discovery ,Nonalcoholic fatty liver disease ,medicine ,Autophagy ,Humans ,Medicine, Chinese Traditional ,ved/biology ,Chemistry ,Plant Extracts ,Eucommiaceae ,Fatty liver ,Hep G2 Cells ,medicine.disease ,Lipid Metabolism ,In vitro ,Molecular Docking Simulation ,PPAR gamma ,Plant Leaves ,medicine.drug ,Drugs, Chinese Herbal - Abstract
Ethnopharmacological relevance Eucommia ulmoides (E. ulmoides) leaves are included in the Chinese Pharmacopoeia, and are traditionally used to treat hypertension, obesity, diabetes, and other diseases. Numerous pharmacological studies have shown that E. ulmoides has a good effect on lowering blood lipids and can improve obesity and nonalcoholic fatty liver. Aim To study the mechanism of E. ulmoides leaves in regulating nonalcoholic fatty liver disease by combining prediction and validation. Methods Using network pharmacology, and molecular docking to predict E. ulmoides in regulating the action mechanism and potential active ingredients of nonalcoholic fatty liver, large hole adsorption resin enrichment active sites, in vitro experiments were performed to verify its fat-lowering effect and mechanism. Results The major components of E. ulmoides leaves exhibited good combination with lipid metabolism-regulating core proteins, particularly flavonoids. EUL 50 significantly reduced lipid accumulation, and increased PPARγ. Compared with the control group, the autophagy level increased after the administration of EUL 50. PPARγ decreased significantly after the addition of chloroquine (CQ, autophagy inhibitor). Conclusion The active ingredients in E. ulmoides leaves regulating nonalcoholic fatty liver disease are mainly flavonoids and phenolics. EUL 50 may play a role in lowering lipids by regulating PPARγ expression through inducing autophagy.
- Published
- 2021