1. Single nucleotide polymorphism scanning and expression analysis of ACSL1 from different duck breeds
- Author
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Yang Zhang, Hongliang Zhang, Guobin Chang, Hao Zhang, Zhixiu Wang, Qianqian Song, Guohong Chen, Xiangxiang Li, and Qi Xu
- Subjects
Food Animals ,Chemistry ,Expression analysis ,Gene expression ,Animal Science and Zoology ,Lipid deposition ,Single-nucleotide polymorphism ,Molecular biology ,Deposition (chemistry) ,Gene - Abstract
Accumulating studies have indicated that the long-chain fatty acyl-CoA1 (ACSL1) gene is related to fat deposition and meat quality in mammals. However, few studies have investigated the relationship between ACSL1 and lipid deposition in ducks. To examine this, we assessed the physicochemical property, homologous alignment, and phylogenetic analyses of the ACSL1 amino acid sequence using bioinformatics tools. The analysis indicated that the ACSL1 amino acid sequence varies in animals, and the duck ACSL1 protein is most closely related to that of chicken. Two single nucleotide polymorphism (SNP) sites were identified at 1749 and 1905 bp of the coding region of ACSL1 by sequencing. Quantitative real-time PCR and western blotting were used to measure mRNA and protein levels in abdominal fat, breast muscle, and liver tissue of Pekin duck (BD) and Cherry Valley duck (CD). mRNA and protein expression were significantly higher in BD than in CD in abdominal fat and liver tissue (P
- Published
- 2022
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