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The redistribution of soil and associated organic matter across landscape represents a major perturbation to the carbon cycle because the established colluvial soils change the levels of C mineralization and sequestration. In this study, two profiles of a colluvial soil 4 m deep were analyzed to test whether its two layers produced by erosion differ from the organic layer of the original soil and if the microbial characteristics correspond to the soil properties. The structure of microbial communities was assessed by both quantitative PCR and Illumina amplicon sequencing. Microbial activities were determined by hydrolytical enzymes. The bacterial community structure was correlated with vertical gradients of soil chemical properties. The dominating bacterial phyla were the same along the whole profile but their relative abundance changed. The upper horizon determined by tillage and reaching to approx. 75 cm had highest values of dissolved organic carbon, P and K and was characterized by Proteobacteria and Bacteroidetes . Also, the activities of hydrolytical enzymes occurred mostly there. The second horizon of deposited soil reaching to approx. 250 cm was characterized by Acidobacteria and Gemmatimonadetes . The lowest horizon of buried Chernozem was characterized by increased soil organic carbon, manganese, iron and sulfate and characterized by Nitrospirae and Rubrobacteria . The community analysis indicated that chemolithotrophic processes might be important in these buried horizons so the decomposition may be slower and residence times for these deep carbon pools longer than in the original upper horizons. In these colluvial systems, erosion could lead to soil organic C stabilization.

Mitochondrial calcium-independent phospholipase A2γ (iPLA2γ) belongs to a family of enzymes that participate in cellular signaling by simultaneously producing free fatty acids and lysophospholipids. We have shown previously that the activity of iPLA2γ is increased following the addition of oxidants, such as tert-butyl hydroperoxide (tBHP) or H2O2. We have also shown that iPLA2γ-liberated fatty acids induce H+ transport mediated by mitochondrial uncoupling proteins and mitochondrial adenine nucleotide translocase (ANT) in heart, lung and spleen mitochondria, leading to a decrease in the mitochondrial protonmotive force and subsequent decrease in mitochondrial superoxide production [1,2]. Here we tested the hypothesis that iPLA2γ serves similar antioxidant function in brain. Using brain mitochondria isolated from wild-type (WT) mice, we found that low micromolar concentrations of tBHP induced increase in respiration and a parallel decrease in mitochondrial superoxide and H2O2 production. These effects of tBHP were fully prevented by bovine serum albumin and inhibited by R-bromoenol lactone, a selective inhibitor of iPLA2γ, and were absent in brain mitochondria isolated from iPLA2γ-KO mice. Moreover, the tBHP-induced effects were fully inhibited by carboxyatractyloside, indicating the participation of ANT. In addition, we observed decrease in mRNA levels of selected antioxidant enzymes and increase in lipid peroxidation products in brain tissues homogenates of iPLA2γ-KO mice compared to the WT controls. Our results support the hypothesis of an antioxidant role of iPLA2γ in brain, consistent with iPLA2γ-dependent, ANT-mediated attenuation of mitochondrial superoxide production. Supported by LTAUSA17174