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Chronic stress can have an adverse effect on behavior changes and damage to the pyramidal neurons of the brain. Pyramidal neurons of the brain play an important role in awareness, decision making, cognitive function, spatial memory formation and working memory. The effects of chlorogenic acid (CGA) found in arabica coffee can affect the improvement of cognitive function, working memory, and histology of brain pyramidal neurons. The content of chlorogenic acid (CGA) in coffee has active compounds that can maintain the number of brain pyramidal neurons due to stress. The aim of this study was to determine the effect of arabica coffee extract on the average number of brain pyramidal neurons exposed to UCS stress. The research method used was a post-test experimental study with controlled group design. A total of 24 adult zebrafish (3 months old) were divided into 4 groups. Four groups (KN, KP, K25, K50) were acclimatized (1 week) first, for groups K25 and K50 were given arabica coffee extract as much as 25mg / dl and 50 mg / dl for 4 days before inducing unpredictable chronic stress (UCS). The results of the study proved that the extra ethanol group of arabica coffee at a dose of 50 mg / dl could maintain the number of pyramidal neurons in zebrafish treated with modified UCS stress (P

Spent coffee grounds are one of the most interesting waste streams of coffee industry containing antioxidants, whose extraction may represent an innovative solution for the reduction of the environmental burden of this waste, simultaneously providing high added value compounds. The aim of this work was to valorize SCG (Coffea arabica L.) using a non-conventional technique (high pressure and temperature extraction), by providing pivotal information and experimental data essential for the design of a profitable and green process. A 3-level factorial design was firstly employed to verify the combined effects of ethanol concentration in water, used as solvent, and temperature on total polyphenol yield and antiradical power of the extract. Desirability method was used to estimate the optimal conditions (ethanol 50% (v/v) as solvent at 150 °C), allowing the recovery of remarkable amount of bioactive compounds (30.4 ± 1.7 mg/100 mL of chlorogenic acid and 31.7 ± 2.9 mg/100 mL of caffeine). Moreover, Peleg's model was successfully applied to describe extraction kinetic, showing that an extraction time of 60 min is sufficient to produce extracts with high extraction yields (43 ± 0.8 mg caffeic acid equivalents/g dried biomass) and remarkable antiradical properties (734 ± 11 μg trolox equivalents/g dried biomass).

El objetivo fue establecer un protocolo para la desinfección y el establecimiento de microesquejes de café Coffea arabica Var. Caturra Rojo provenientes de campo, como etapa previa para la multiplicación in vitro. El diseño utilizado fue el diseño en bloques completos al azar (DBCA), los tratamientos para desinfección fueron t1, t2 y t3; mientras que, en la fase de establecimiento fueron t1, t2, t3, t4 y t5. Se encontró que el protocolo de desinfección que mejor funcionó fue t3 que utilizó benomilo y gentamicina por 10 minutos, luego el uso de hipoclorito de sodio, lejía, a 1,3 % por 30 minutos, en base a que favoreció una mayor sobrevivencia y menor fenolización de los explantes. El mejor medio de establecimiento fue el t5 que tuvo como ingredientes MS más BAP a 0,5 mg l-1.

Coffea arabica sediment, a popular agro-by-product from the coffee juice processing industry, contains many phytochemicals, and it is often discarded as waste. The antioxidant and anti-tyrosinase activities of an ethanol extract and its active fractions obtained from Coffea arabica sediment was studied. Additionally, whiteleg shrimp (Litopenaeus vannamei) was treated with the extract and fractions, and the quality during refrigerated storage was evaluated. The most active fraction (P

BACKGROUND: Coffee arabica gayo leaf extract (Coffea arabica L.) contains polyphenols (chlorogenic acid), flavonoids, saponins, tannins, and steroids which improved PI3K serum levels in rats type 2 diabetic rat. AIM: The purpose of the study was to determine the effect of Coffee Arabica Gayo Leaf Extract (C. arabica L.) on increased PI3K serum levels in type-2 diabetic rat (Rattus norvegicus). METHODS: Thirty-five male Wistar rats with type 2 diabetic induced a combination of feeding a high-fat diet for 5 weeks and intraperitoneal injection of low dose streptozotocin (30 mg/kg). The diabetic rats were randomly divided into seven groups which consist of group without type 2 diabetic, group without type 2 diabetic with dose 250 mg/kg/day Coffee Arabica Gayo Leaf Extract, group with type 2 diabetic without intervention, group with type 2 diabetic with metformin, and group with type 2 diabetic with Coffee Arabica Gayo Leaf Extract doses 150, 200, and 250 mg/kg/day. The extract was administered orally for 30 days. Subsequently, PI3K serum levels were measure by Sandwich Elisa. RESULTS: There were significantly higher PI3K serum levels in the treatment groups than in the control groups (p = 0.037) after giving Coffee Arabica Gayo Leaf Extract 200 mg/kg/day and 250 mg/kg/day to rats for 30 days. CONCLUSION: These data suggest that Coffee Arabica Gayo Leaf Extract can improve PI3K serum levels in a rat model type 2 diabetic.

Arabica coffee seeds contain a composition of Caffeine, Chlorogenic acid, Flavonoids, and Trigonelline. The purpose of this study is to test the antibacterial of Arabica coffee seed extract against bacterium Enterococcus faecalis at concentrations of 1,5625%, 3,125%, 6,25%, 12,5%, 25%, 50% and 100%. The extraction method using maceration with solvent 96%. Antibacterial activity test was done by using the Kirby and Bauer diffusion test method. The results showed that Arabica coffee seed extract could provide inhibition starting from 3,125% with an average diameter of inhibition zone of 1,16 mm to the largest concentration of 100% with an average zone diameter of inhibition of 14,6 mm. At the same time, the average diameter of the inhibitory zone of antibiotic ampicillin at a concentration of 1% as a control (+) is 24,6 mm. The results showed that the greater concentration, the greater the inhibitory zones are formed. Keywords: Coffea arabica, Enterococcus faecalis, antibacterial, inhibition zone

BACKGROUND: Diabetes mellitus (DM) continues to increase with currently more than 463 million people in the world live with DM. One of the causes of Type 2 DM (T2DM) is insulin resistance. Determining laboratory-based tests for early identification of T2DM is important. One of the tests developed was the detection of homeostatic model assessment – insulin resistance (HOMA-IR) levels. AIM: Using chlorogenic acid found in coffee leaves as antidiabetic agent for alternative treatments in DM, this research is conducted to understand the effect of Coffee Arabica Gayo Leaf Extract (Coffea arabica L.) in decreasing HOMA-IR levels in T2DM rats. MATERIALS AND METHODS: Thirty-five male Wistar rats (Rattus norvegicus) had T2DM induced using a combination of feeding a high-fat diet for 5 weeks followed by multiple intraperitoneal injections of low-dose streptozotocin (30 mg/kgBW), divided into 7 groups (2 groups that did not receive treatment [K1 and K3] and 5 groups that received treatment [K2, K4, K5, K6, K7]). The extract was administered with dosage 150, 200, and 250 mg/kgBW/day through a nasogastric tube for 30 days. Then, the HOMA-IR value will be obtained by multiplying fasting plasma insulin by fasting plasma glucose, then divide by a constant of 22.5. RESULTS: The study showed a significant difference before and after treatment, p value was < 0.05, which a decrease HOMA-IR levels (p = 0.002) after given Coffee Arabica Gayo Leaf Extract at 200 and 250 mg/kgBW/day to Wistar rats for 30 days. The decrease HOMA-IR levels are greater than The T2DM group that received metformin and group without treatment. CONCLUSION: Coffee Arabica Gayo Leaf Extract can significantly decrease HOMA-IR levels at a dose of 200 and 250 mg/kgBW. The decrease of HOMA-IR levels is greater than The T2DM group that received metformin and group without treatment.

Somatic embryogenesis in Coffea spp. is important for mass production and genetic engineering. Coffee is an exceptional case, as somatic embryogenesis can be induced by applying a cytokinin such as 2iP as the only plant growth regulator. 1-(2-(2-Hydroxyethyl)phenyl)-3-(3-(trifluoromethoxy)phenyl)urea (3TFM-2HE) and 1-(3-bromo-5-(trifluoromethoxy)phenyl)-3-(2-(2-hydroxyethyl)phenyl)urea (3TFM,5Br-2HE) are two newly designed cytokinin oxidase/dehydrogenase inhibitors (CKX) from the diphenylurea group. We used a Coffea arabica leaf disks bioassay to demonstrate the indirect somatic embryo induction potential of these compounds. The leaf disks were incubated on modified Murashige and Skoog (MS) semi-solid medium in which 3TFM-2HE or 3TFM,5Br-2HE were combined with N6-(2-isopentenyl)adenine (2iP). Although these compounds do not possess intrinsic cytokinin activity, they enhanced the activity of 2iP, resulting in direct somatic embryogenesis after seven weeks. The best results were obtained with 1 µM 3TFM-2HE and 5 or 10 µM 2iP. Maturation of somatic embryos into fully developed plants took place on medium supplemented with 0.5 µM kinetin and the somatic embryos developed true leaves and a root system.

The current work assessed the phytochemical contents of Arabica whole coffee cherry (WCC) and its two commercially available extracts: a minimum 40% chlorogenic acid (CGA; WCCE-1) and 70% caffeine (WCCE-2). Mass spectrometry analyses tentatively identified 219 phytochemicals in the three coffee samples, which is, so far, the largest number of identifications in a single study. A new group of CGA derivative namely caffeoylvaleroylquinic acid (CVQA) was identified in the three samples. Moreover, three 5-hydroxytryptamide derivatives (C20-5HT, C22-5HT, and C24-5HT) were identified in WCC but not in the extracts. Two groups of atractyligenin derivatives (carboxyatractyligenin and noncarboxyatractyligenin) were identified in the three samples. Furthermore, our results show that both extracts retained a large number of phenolic and other potentially bioactive compounds that were naturally present in whole coffee cherry (WCC).

В данной статье исследуются показатели общего содержания фенолов, флавоноидов, антирадикальной активности по методу DPPH, восстанавливающей силы по методу FRAP для экстрактов зёрен чёрного обжаренного кофе Арабика (Coffea arabica) и зелёного кофе Робуста (Coffea canephora). Исследовались экстракты, полученные с использованием различных видов растворителей: вода, смесь вода-этанол (96%) (75% об. /25% об.), смесь вода-этанол (50% об. /50% об.), смесь вода-этанол (96%) (25% об. /75% об.) и вода-этанол (96%). Определен тип растворителя, позволяющий максимально полно экстрагировать антиоксидантные вещества из кофе. Максимальные значения общего содержания фенольных веществ обнаружены в экстракте кофе Робуста на основе системы растворителей вода-этанол (75/25), для экстрактов чёрного кофе максимальные значения получены при его экстрагировании водой. Наивысшие показатели общего содержания флавоноидов установлены для экстрактов кофе Робуста и Арабика на основе воды. Низкая антирадикальная активность обнаружена в кофе Арабика и Робуста при соотношении растворителей вода-этанол (25/75). Наибольший показатель восстанавливающей силы обнаружен в экстракте кофе Робуста на основе воды, для получения высоких показателей восстанавливающей силы в экстрактах кофе Арабика в качестве растворителя рекомендуется использовать этанол. Таким образом, можно рекомендовать систему растворителей вода 50% - этанол 50% для получения экстрактов из зёрен чёрного обжаренного кофе Арабика (Coffea arabica) и зелёного кофе Робуста (Coffea canephora), обладающих способностью тормозить окислительный стресс в организме человека.

The purpose of the research was to evaluate the teratogenic effect caused by theprovision of Arabica coffee solution with variations of 0.36 ml, 0.72 ml and 1.08 ml withpositive control of caffeine at a dose of 300 mg / kg bw to rats during organogenesis. Themeasurement parameters in this study are the appearance of reproduction, externalmalformations and scalal malformations. The results in this study found that theadministration of Arabica coffee solution with a volume of 1.08 ml and caffeine 300 mg /kg bb caused an abnormality in the reproductive appearance of weight loss and body length,whereas for extrenal malformation and scalal malforation no abnormalities were found ineach administration of the solution Arabica coffee and caffeine.

Coffea arabica (Arabica) and C. canephora (Robusta) are the most popular coffee species in the world, accounting for 99% of overall consumption of coffee beans. Arabica generally possesses better coffee quality than Robusta partly due its higher sucrose content. The flavor and taste (FT) of coffee, as important aspects of coffee quality, are mainly affected by the content of caffeine, phenolic acid and terpenoid compounds, which use sucrose as an important precursor. However, how sucrose affects the coffee FT remains unclear. In this study, coffee beans at different developmental stages from Arabica #161 (A161, high-sucrose variety) and Robusta #6 (R6, low-sucrose variety) were sampled to perform transcriptomic analysis. Most differentially expressed genes (DEGs) between them were enriched in sucrose-related metabolisms and the FT-related metabolism processes: caffeine biosynthesis, phenylpropanoid and flavonoid pathway, terpenoid metabolism. Thirty-four candidate DEGs probably contributed to the higher content of sucrose, anthocyanin and linalool in A161, and higher content of caffeine and carotenoid in R6. Generally, sucrose-related metabolisms were strongly associated with FT-related substance accumulation. The content of sucrose and its influence on the downstream secondary metabolism probably play important roles in the FT quality of coffee beans. Our results provide efficient targets for investigation regarding the influence of sucrose on FT quality of coffee beans.

Coffee has a significant economic, social, and cultural impact on Brazilian society, generating jobs for thousands of Brazilians. Good management practices such as weed control have direct and indirect benefits on coffee yield and quality. Currently, there is an increase in the infestation rate of Digitaria insularis in coffee plantations due to chemical resistance to glyphosate. In the literature, the study of the combination of glyphosate with different herbicides has been investigated, aiming at improving the efficiency of the control of Digitaria insularis. The objective of this work was to evaluate the efficiency of control of the Digitaria insularis in a coffee farm using a combination of glyphosate with clethodim and phenoxaprope-p-ethyl. Trials were conducted in a commercial coffee farm in southern Minas Gerais in a field with a two-year-old cultivar IAC Catuai 144. The experiment was conducted under a completely randomized design with five treatments and four replications, totaling 20 experimental plots. Thirty and ninety days after application, the plant numbers were counted and the visual analysis with the use of drones and the digital camera. The results show that the use of glyphosate alone was not efficient in the power of Digitaria insularis. The results show that the herbicide mixtures were efficient in the control. Key words: Chemical control; Herbaceous; Weeds; Mixtures; Active ingredients; Coffea arabica.

Genetic coffee breeding is aimed at increasing yield associated with tolerance or resistance to biotic and abiotic factors, besides providing a better beverage quality and supplying the demand for bigger beans. The efficiency in photosynthetic activity can limit produce and diversify genotypes, mainly under adverse environmental conditions. Consequently, the importance of selection of Coffea arabica L. regarding these characteristics stands out. Therefore, the objective of this paper was to measure the physiological characterization and yield of Coffea arabica L. progenies with large beans “Big Coffee VL”. Twelve productive progenies were selected and classified according to fruit size (“small”, “medium” and “large”), which were: S14, S23, S34, S36, M4, M5, M14, M20, L10, L12, L17 and L31. Net photosynthetic rate (A), stomatal conductance (gs), transpiration rate (E), water use efficiency (WUE), internal carbon (Ci), intercellular CO2 concentration in the mesophyll were evaluated on the current external CO2 concentration (Ci/Ca), besides vapor pressure deficit (VPD), leaf temperature (Tleaf), indirect determination of the relative levels of chlorophylls a, b and total, in addition to bean yield in two crops. The Tocher grouping resulted in the formation of 4 groups, and progenies M4, L10 and S34 remained in isolated groups. Progeny L10 stood out for higher mean values of A, gs and Ci; the opposite behavior was observed in progeny S34. Progeny M4 is noteworthy as the one with the highest yield in the two years considered, in addition to presenting high photosynthetic rate and chlorophyll indexes. It is concluded that the progenies of “Big Coffee VL” show variability for physiological parameters and productivity. Progenies S14, M4 and L10 stood out, and S14 was more efficient in the use of water; M4 was the most productive and L10 stood out in terms of gas exchange. Key words: Chlorophyll; Coffea arabica L.; Gas exchange; Genetic breeding.

Phenolic compounds, such as chlorogenic acid, caffeine, sugars, vitamins, minerals and volatile substances are major compounds of coffee (Coffea ssp.). They perform distinctive biological functions and are classified as nutraceuticals and insecticides. In at least one phenological stage, coffee leaves are more likely to be used for commercial purposes. The objective of this study was to analyze leaf area, content of chlorophyll a, b and total, caffeine, chlorogenic acid (5-CQA), sugars and macro and micronutrients in arabica coffee leaves (Coffea arabica) var. Caturra rojo grafted on robusta coffee (Coffea canephora) var. Romex during an annual cycle. The quantification of chlorogenic acids, caffeine and reducing sugars was analyzed by chromatography (HPLC). Nutrients were quantified by atomic absorption spectrophotometry and we performed one-way factorial designed ANDEVA and Tukey means comparison test. Sampling of 10 trees was done completely at random, and the samples in chromatographic analyses were run in triplicate. The largest values occurred as follows: leaf area in the blooming and fruiting stage; chlorophyll a, b and total during the vegetative stage; caffeine in the blooming stage; 5-CQA was obtained during the vegetative stage, in which we observed the highest concentrations of glucose and fructose. The concentration of macronutrients, except Mg, was higher during the fruiting stage, while Fe and Zn were higher during blooming and Mn and B in fruiting. Macro and micronutrients accumulated during the fruiting stage. Coffee leaves may be both functional and nutraceutical during the vegetative and fruiting stages.

The establishment of new coffee plantations requires vigorous and healthy seedlings that guarantee good growth and high yields; hence the importance of studying the effect of bio-stimulant substances in the early stages of the crop's ontogeny. In this sense, the combined effect of five concentrations of salicylic acid (0; 0.0125; 0.025; 0.05; 0.1 and 1 mM) and different times of imbibition of the seeds (1, 2, 3 and 4 h), on the germination and initial growth of coffee plants (Coffea arabica L.) variety Costa Rica 95 was studied. The experimental design was completely randomized with a bifactorial arrangement, with 24 treatments and 20 repetitions. The percentage and germination rate, height and diameter of the hypocotyl were evaluated. The results showed that the concentrations (0.0125; 0.025 and 0.05 mM) and imbibition times of the seeds of 3 and 4 h in salicylic acid promoted an early germination and a positive effect on the height and diameter of the hypocotyl. The findings show that salicylic acid applied in low concentrations can be used to accelerate the germination of coffee seeds and induce the initial growth of coffee plantlets, while it could be considered as a viable option for coffee producers, both for its easy application and the safety of the product.

Epidemiological and experimental studies have found that the consumption of regular coffee has various health benefits. In the present study, we investigated the effect of decaffeinated coffee extracts on the inflammation process and found that aqueous extracts of green and roasted coffee have anti-inflammatory activity. The anti-inflammatory and antioxidant effects of aqueous extract of decaffeinated coffee (AD) and ethanol extract of decaffeinated coffee (ED) were evaluated in animal models using a DPPH radical scavenging test. In the formalin test, the extracts reduced licking activity in both late phases. The inhibitory values of oedema 3 h post-carrageenan indicated an anti-inflammatory effect. In the paw pressure test, the animals treated with extracts exhibited a reduction in the withdrawal threshold. These results provide evidence for the anti-inflammatory and antioxidant properties of decaffeinated coffee extracts that can be attributed to the potential of compounds present in coffee.

La giberelina es conocida como una fitohormona que participa activamente en el control de la floración de varias especies. En el presente estudio se evaluó la aplicación exógena de ácido giberélico (0.1% y 0.5%) a 2959 plantas de Coffea arabica L., variedad Castillo cultivar Rosario; con el objetivo de establecer la relación existente entre la floración del café y el número de entrenudos inducidos en la rama principal media y superior mediante la aplicación foliar de la hormona. El proyecto se desarrolló en la Finca “El Limón” del Municipio de Buriticá (Antioquia) durante los meses de agosto y febrero. Independientemente de la concentración de fitohormona aplicada, como resultado de la aplicación exógena se encontró un aumento en el número de entrenudos y en la producción de brotes florales. Finalmente determinar el análisis de significancia, los datos obtenidos en los diferentes parámetros fueron analizados mediante la prueba estadística T-Student del 95%.

Growing water restrictions associated with climate changes constitute daunting challenges to crop performance. This study unveils the impacts of moderate (MWD) or severe (SWD) water deficit, and their interaction with air [CO2], on the photosynthetic apparatus of Coffea canephora cv. Conilon Clone 153 (CL153) and C. arabica cv. Icatu. Seven year-old potted plants grown under 380 (aCO2) or 700μLL−1 (eCO2) [CO2] gradually reached predawn water potentials between −1.6 to −2.1MPa (MWD), and below −3.5MPa (SWD). Under drought, stomata closure was chiefly related to ABA rise. Increasing drought severity progressively affected gas exchange and fluorescence parameters in both genotypes, with non-stomatal limitations becoming gradually dominating, especially regarding the photochemical and biochemical components of CL153 SWD plants. In contrast, Icatu plants were highly tolerant to SWD, with minor, if any, negative impacts on the potential photosynthetic functioning and components (e.g., Amax, Fv/Fm, electron carriers, photosystems (PSs) and RuBisCO activities). Besides, drought-stressed Icatu plants displayed increased abundance of a large set of proteins associated with the photosynthetic apparatus (photosystems, light harvesting complexes, cyclic electron flow, RuBisCO activase) regardless of [CO2]. Single eCO2 did not promote stomatal and photosynthetic down-regulation in both genotypes. Instead, eCO2 increased photosynthetic performance, moderately reinforced photochemical (PSs activity, electron carriers) and biochemical (RuBisCO, Ru5PK) components, whereas photoprotective mechanisms and protein abundance remained mostly unaffected. In both genotypes, under MWD, eCO2 superimposition delayed stress severity and promoted photosynthetic functioning with lower energy dissipation and PSII impacts, whereas stomatal closure was decoupled from increases in ABA. In SWD plants most impacts on the photosynthetic performance were reduced by eCO2, especially in the moderately drought affected CL153 genotype, although maintaining RuBisCO as the most sensitive component, deserving special breeder’s attention to improve coffee sustainability under future climate scenarios.

The application of glyphosate to coffee crops can cause injuries to plants. Fertiactyl® foliar fertilizer reduces injuries when mixed with glyphosate; however, it is important to establish which me...

Cold-stressed leaves possess an elevated risk of photodamage due to an inefficient photosynthetic apparatus during winter conditions. Recovery of cold-stressed coffee seedlings treated with different concentrations of nitrogen applied as foliar urea sprays [control (0), 5, 10, 20, and 40 mM], was investigated under optimum growth chamber conditions (25/20°C) for three months. Concentrations of nitrogen and photosynthetic pigments in the leaves increased with increasing concentration of the foliar sprays up to 20 mM. This was accompanied with a recovery of the photosynthetic apparatus and increased net carbon assimilation rate. In addition, 10 and 20 mM-treated plants also had the highest maximal efficiency of PSII compared to their lower or higher nitrogen concentration treated counterparts. Conversely, these plants contained lower concentrations of 5-caffeoylquinic acid, mangiferin, trigonelline, and caffeine than control plants. We concluded therefore that foliar sprays of the appropriate nitrogen concentration were adequate for recuperating the photosynthetic apparatus and improved the photosynthetic performance of the cold-stressed coffee seedlings.

Bondowoso district is the predominant supplier of coffee beans, and also known as Republik Kopi. However, there was still insufficient data about the chemical characteristics of coffee from Bondowoso. This research has main aims to characterize the chemical characteristic of Coffea Arabica L. from Bondowoso, and determine the impact of traditional fermentation on them. Coffee beans were naturally-fermented through soaking in water for less than 12 hours. Unfermented coffee beans were used as a control. Both unfermented and fermented coffee beans were subjected to chemical analysis. Results show that total nitrogen and lipid contents of both fermented and unfermented beans decreased after fermentation into 0.19%±0.03 and 10.03%±0.14, respectively. LCMS analysis of coffee beans revealed that the majority of amino acid contents in fermented beans were higher than that found in unfermented beans, while caffeine and derivatives to be lower in fermented coffee beans than in unfermented beans. One exception for this was 7-methylxanthine, which was found only in fermented beans. Other metabolites, including procyanidines A and B, were found to decrease through fermentation. Interestingly, 3-flavanol was found only in fermented coffee beans. This research results would benefit on improving the quality of coffee through fermentation step.

As seed chemical composition may lead to different responses during drying and other post-harvest operations, the objective of this study was to determine the effect of drying rate (slow and rapid) and the concentration and composition of lipids in the embryo and endosperm tissues of Coffea arabica seeds on desiccation tolerance/sensitivity. The total concentration of lipids and the composition of fatty acids were evaluated in isolated embryos and endosperms, and in whole (intact) seeds. Embryos had a higher total lipid concentration (23%) than endosperm tissue (8%). Linoleic acid was the predominant fatty acid, with concentrations of 48% in the endosperm and 34% in the embryo tissues. Although rapid or slow drying did not change the fatty acid profile in the embryos or endosperms, the palmitic and linoleic acid concentrations varied significantly between the embryo and endosperm. The predominance of linoleic acid in the endosperm supports the hypothesis that the endosperm tissue may be an important source of damage to the seed and may advance seed deterioration. This indicates that unsaturated fatty acids may have a greater effect on sensitivity to desiccation than the percentage of total lipids. Made available in DSpace on 2020-05-16T04:38:13Z (GMT). No. of bitstreams: 1 SST-47-3-Article-19-018-Guimara771es.pdf: 4828865 bytes, checksum: 04cfe8ec64baa282ec593ea86a4975c0 (MD5) Previous issue date: 2020

Diabetes mellitus is an excessive level of blood sugar concentration disease. One of the compounds that can moderate the blood sugar level is chlorogenic acid that mostly found in green coffee. This study aims to determine the extract activity of Arabica (Coffea arabica L.) green coffee in decreasing the blood sugar level of mice (Mus musculus) that induced by alloxan monohydrate. The method is True Experimental Laboratories with the analysis of chlorogenic acid levels using a UV-Vis spectrophotometer. The research design is Post Test Control Group Design, using 24 mices that divided into six treatment groups. The groups consist of negative control (distilled water), Dose I (100 mg/kg bw), Dose II (200 mg/kg bw), Dose III (300 mg/kg bw), Dose IV (400 mg/kg bw), and positive control (metformin). Blood sugar levels of the mice is measured at the intervals of 1st day, 7th day, 21st day. The data is analyzed using the SPSS program with normality tests (Kolmogorov-Smirnov), homogeneity tests (Levene Test), and parametric statistical tests (One-Way ANOVA). The data results was normally distributed and homogeneous (P> 0.05) with several pairs of data in the treatment group were significantly different (P> 0.05). The extract of Arabica (Coffea arabica L.) green coffee contained 4.235% chlorogenic acid, could reduced the hyperglycemia mice blood sugar levels by the dose of 10.72% (Dose I); 19.85% (Dose II); 27.20% (Dose III); and 31.60% (Dose IV) with dose IV as the effective dose.

Coffee is one of the oldest and most consumed beverages. The raw material for obtaining a good quality coffee drink is the grain. Bioactive compounds and organic acids in coffee beans are reflections of a series of attributes that, together, confer to the coffee peculiar flavor and aroma. This study aimed to identify trigonelline, chlorogenic acid, caffeine and organic acids in green coffee beans, and to validate the chromatographic method using High-Performance Liquid Chromatography (HPLC). Coffea arabica green coffee beans were used in this experiment. Trigonelline, chlorogenic acid, caffeine, and organic acids were identified and quantified by HPLC. Selectivity, linearity, limit of detection, limit of quantification, precision, and accuracy were used to validate the methods. Three bioactive compounds (trigonelline, chlorogenic acid, and caffeine) and seven organic acids (oxalic, citric, malic, quinic, succinic, lactic, and acetic acids) were quantified in the samples. The mean values for trigonelline, chlorogenic acid (5-ACQ), and caffeine ranged from 1.28 to 1.66, 3.47 to 4.73 and 1.26 to 1.59 g 100g-1, respectively. The values of organic acids, as well as the phenolic compounds, agree with those reported by the literature for green coffee beans of C. arabica. The validation parameters allowed the method to be considered linear, exact, and precise. Therefore, it resulted in a method reliable for studies of the composition of food matrix or quality control of green coffee beans.

Coffee quality is a complex trait involving sensory and bean characteristics as well as biochemical contents. The objective of this study was to assess the major factors influencing the quality of wild Arabica coffee (Coffea arabica L.) in the natural coffee forests of southwest and southeast Ethiopia. Results revealed that both natural (soil, aspect, elevation, climate, geographic location) and human factors (cherry harvesting/ handing, theft, forest management) considerably influenced the quality of wild Arabica coffee. The soil factor affected every component of coffee quality (cup quality, bean characteristics and biochemical contents). The cup quality of coffee varied with soil properties, especially with available P and soil texture. The bean size distribution was also affected by soil properties; there was significant positive relationship between soil pH, sand or Mn and the proportion of bold beans (retained on screen 17). Soil organic matter, total N and sand content were inversely correlated with caffeine content, but available P and clay content were positively correlated with caffeine. Increase in elevation led to increase in bean size up to the elevation of about 1600 m above sea level, but thereafter no more increase in bean size (hump-shaped relationship, not monotonic). Bean size increased with increase in longitude, but it decreased with increase in latitude. Cup quality was also significantly influenced by coffee harvesting and handling, but its influence was not noticed on bean size and biochemical contents. Coffee quality is therefore the resultant of an interaction of different natural and human factors prevailing in the respective area. Key words: Arabica coffee, bean size, biochemical content, cup quality, environment, management/handling.

FAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Sensory analysis is an important tool to control and improve the quality of food products. This study aimed to evaluate the sensory attributes and consumer preference of coffee beverages prepared from roasted ground coffee (Coffea arabica) of different brands sold in Minas Gerais, Brazil, using projective mapping and assess the relationship of sensory attributes and consumer preferences with physicochemical properties. Titratable acidity, pH, soluble solids, color, crude fat, protein, total phenolic content, caffeine, trigonelline, 5-caffeoylquinic acid and caffeic acid were determined. Physicochemical properties showed good agreement with projective mapping data. Samples associated with high acidity in the projective mapping task had the highest levels of 5-caffeoylquinic acid. Coffees associated with bitter/roasted taste and darker colors had typical physicochemical characteristics and color properties of dark-roasted coffee. The sample perceived as thin had the lowest soluble solids content. Consumers preferred traditional, dark-roasted coffee over gourmet, light-roasted coffee. Projective mapping is an effective technique for assessing the sensory attributes of brewed coffee. A avaliação dos atributos sensoriais pode ser uma ferramenta importante para controlar e melhorar a qualidade de produtos alimentícios. Este trabalho teve como objetivo descrever sensorialmente amostras de café torrado e moído da espécie arábica de diferentes marcas brasileiras por teste descritivo avançado (projective mapping) e relacionar os dados obtidos com resultados físico-químicos. Foram analisados acidez titulável, pH, sólidos solúveis, cor, lipídeos, proteínas, compostos fenólicos, cafeína, trigonelina, ácido 5-cafeoilquínico e ácido caféico. Os resultados obtidos nas análises físico-químicas foram coerentes com os atributos relacionados aos cafés no projective mapping. Os cafés agrupados no projective mapping próximos ao atributo acidez foram os que apresentaram os maiores valores de ácido 5-cafeoilquínico. Os cafés associados ao sabor amargo/torrado e cor concentrada apresentaram cor mais escura na análise colorimétrica e características físico-químicas de torrefação escura. O café considerado bebida diluída foi o que apresentou menor valor de sólidos solúveis. Os resultados mostraram que o projective mapping é eficiente para análises sensoriais descritivas de café e que o público testado tem preferência por cafés tradicionais com torrefação escura.

Introduction: Ischemic stroke is the third leading cause of death worldwide. The purpose of the current study was to assess the preliminary effects of Coffea arabica (CA) in experimentally-induced stroke in an animal model in the context of neuroprotection. The study was also designed to document the prophylactic use of CA in patients experiencing stroke symptoms. Methods: A total of 25 male albino rats, 12 months of age, were purchased from the local market. They were acclimatized for seven days and divided into 5 groups. Each group consists of 5 subjects. Each subject was trained on a specific test for behavioral assessment. Behavioral modulation of each rat was performed using four tests, namely cylinder test, staircase test, forelimb flexion test, and pasta test. All the tests were performed as per standard criteria at the 4th, 8th, 12th, and 14th day of drug administration. The subjects were administered with caffeine (2.4 mg/kg) and CA (100 mg/kg and 200 mg/kg extract) doses for 14 days to assess the prophylactic use of CA. After 14 days of treatment, rats were subject to ischemic stroke induction using the middle cerebral artery ligation method. All four tests used for behavior modulation were applied at 24, 48, and 72 hours intervals of postsurgery. The subjects were further sacrificed for histopathological investigations. Statistical analysis was performed with SPSS (V. 22) software using one-way ANOVA. Results: Our findings suggest that treatment with CA, 100 mg/kg and 200 mg/kg orally, decreases the infarct volume. However, there are not many considerable differences that were found in both doses. Histopathological investigations revealed characteristic structural changes occurring in both gray and white brain regions, depending on the severity, location, and duration of the ischemic stroke. Conclusion: CA is a harmless and active mediator in the dissolution of blood clots and the recovery of stroke in rats. It is an agent that has been found to be efficient for brain activity with few side effects and behavioral modifications.

Seven new lactam ent-kaurane diterpenoids, cafemides A-G (1-7), were isolated from roasted beans of Coffea arabica. Their structures were elucidated by extensive spectroscopic analysis including 1D, 2D NMR (heteronuclear single quantum correlation (HSQC), heteronuclear multiple bond correlation (HMBC), 1H-1H correlation spectroscopy (COSY), and rotating frame Overhauser effect spectroscopy (ROESY)), high-resolution electrospray ionization mass spectrometry (HRESIMS), and IR spectra. They were divided into subtype I-III according to the structure. Further, with the aid of liquid chromatography-tandem mass spectrometry (LC-MS/MS) based molecular network, seven (8-14) subtype II diterpenoids were successfully identified. In addition, a variety of other subtypes of N-containing diterpenoids have been proven in roasted coffee. Compounds 1, 2, 3, 5, and 7 showed a moderate inhibitory effect on α-glucosidase with an IC50 value of 8.28 ± 0.62 μM, 38.23 ± 8.87 μM, 28.94 ± 1.42 μM, 12.44 ± 1.37 μM, and 22.2 ± 5.34 μM, respectively. To the best of our knowledge, this is the first time that N-containing diterpenoids have been reported in coffee.

The objective of this study was to better understand the implications of the management of the number of orthotropic stems in Arabica coffee for its cultivation in low-altitude regions (< 500 m), characterizing its effects over the canopy architecture, production and allocation of biomass, photosynthesis and crop yield, as possible strategy for mitigating the stresses of cultivation at low-altitude regions. The experiment was conducted in a region with 124 m of elevation above sea level, studying four treatments with different numbers of orthotropic stems per plant such as one, two, three and four stems. After planting, during the stage of sprout budding, a number of sprouts were established in accordance to the treatments. The experimental design was a randomized block design, with eight repetitions. Parameters of gas exchange, chlorophyll contents, canopy architecture, production and allocation of biomass, and crop yield were evaluated to characterize the plant development. Overall, increasing the number of orthotropic stems per plant promoted leaf density, number of plagiotropic branches per plant, available leaf area per fruit mass, photosynthetic rate, crop yield, proportion of large grains and, possibly, the de-acceleration of the stem aging rate, mainly for the management with four orthotropic stems. It is concluded that the management of the number of orthotropic stems has potential use as a mitigator for the cultivation of Arabica coffee in low-altitude regions, being able to promote higher canopy density and, possibly, enhance microclimate conditions at the canopy level.

Growing coffee faces challenges regarding the development and productivity of plants. To improve coffee tree cultivation and bean productivity and quality, modern techniques of plant biotechnology need to be adopted that allow the development of more productive and resistant varieties. Among these, somatic embryogenesis stands out as a method of great potential for the propagation of coffee plants in vitro. Some genes are directly related to somatic embryogenesis, especially WOX (WUSHEL-related HOMEOBOX) genes, which play a central role in the process. Therefore, the objective of this study was to identify and analyze the expression pattern of WOX genes in arabica coffee, Coffea arabica. In silico analyses identified seven WOX genes in arabica coffee: Coffea arabica WUSHEL-like (CaWUS-like), Coffea arabica WOX1-like (CaWOX1-like), CaWOX3-like, CaWOX4-like, CaWOX5-like, CaWOX9-like and CaWOX11-like. The transcriptional profile of the CaWOX11-like gene suggests that it may be intrinsically related to the embryogenic process and may be used as a molecular marker. In addition, comparisons of the present results with those from the literature indicate that WOX genes are key regulators of somatic embryogenesis in arabica coffee.

Nonuniform flowering leads to uneven ripening of fruits, which impairs harvesting efficiency and the quality of the coffee. The aim of this study was to determine the water deficit level required to break flower bud dormancy of Coffea arabica and to evaluate its effects on gas exchange, photosynthetic pigment levels, coffee yield, and fruit maturation. After a growth period of 18 months in 200 L pots maintained under greenhouse conditions, water deficit treatments were imposed by withholding watering from plants exhibiting at least a 60% rate of "E4 stage" flower buds. When five groups of six coffee plants reached the pre-dawn leaf water potential (Ψwpd) of -0.04, -0.65, -1.43, -1.96, and -2.82 MPa, the leaf gas exchange was measured and leaf disks were collected to quantify the photosynthetic pigment levels, after which, watering was resumed. The rate of opened flowers increased with the reduction of Ypd based on the mathematical model, Y = 67.064 + 20.660 x ln(-Ψwpd). The leaf gas exchange was strongly affected by water deficit levels, without any alterations in the photosynthetic pigment levels. Coffee yield was not affected by the treatments but the ripe stages of cherries increased slightly with the reduction in Ψwpd. The water deficit level applied at the pre-flowering stage determined the percentage of flowering in C. arabica.

Background::Vitamin C (VC) is believed to enhance immunity and is regularly integrated as a supplementary agent during several treatments.Objective::The green (GC) and roasted (RC) coffee (Coffea arabica) aqueous extracts (0, 125, 250 and 500 μg/ml) combined with VC (50 μg/ml) were examined on the cancerous MCF-7 cell line and normal human lymphocytes.Methods::Neutral red uptake assay, comet assay, immunocytochemical reactivity for protein expression and mRNA expression of apoptosis-related genes were performed.Results::A significant (P< 0.05) concentration-dependent increase of apoptotic features, such as morphological changes, and abundant nuclear condensation, altered the expression of p53 and caspase-3 mRNA, down-regulation of Bcl-2 protein as well as the acidic autophagosomal vacuolization in treated cells. The oxidative stress and DNA single-strand breaks were noticed too.Conclusion::These results suggest that coffee in combination with VC undergoes apoptotic anticancer pathway. This supports the integration of coffee and VC as a valuable candidate for anticancer research and treatments.

Post-harvest processing (PHP) modifies the quality of the coffee and increases the value of coffee production. The choice of PHP to apply depends primarily on the available infrastructure, local climatic conditions and the desired end-value. The objective of this study was to evaluate the influence of PHP on the physico-chemical characteristics of green and roasted coffee beans and their sensory attributes. Coffee cherries from IAPAR 59 cultivar were processed as natural coffee (CN), semi-dry coffee (CD), de-pulped coffee (CP) and floating coffee (CF). Total and reducing sugars, phenolic compounds, chlorogenic acids, lipids, proteins, caffeine and water content were determined in coffee beans collected during (10thday) and at the end of each processing. The roasted beans and their sensory attributes were also analyzed. The greatest changes at the ending point of the processes were found in total and reducing sugars, phenolic compounds, chlorogenic acids and lipids contents. The PHP presented different weight loss to achieve the same visual brown color and luminosity. Beverage sensory attributes were influenced by the PHP: CN and CP coffees presented similar intensities of coffee aroma, but higher intensity of green grassy aroma, and taste were found in CF and CN coffees. Aroma and taste precursors were modified during the PHP and these were associated to husk removal of the coffee beans, suggesting an activation of the germination metabolism during the PHP. This study allowed the characterization of the effects of the different post-harvest processing on the roasted coffee beans and provides the foundations to monitor their efficiencies in the future. Made available in DSpace on 2022-01-25T14:00:42Z (GMT). No. of bitstreams: 1 Flavor-precursors-and-sensory-attributes.pdf: 573175 bytes, checksum: 368650d928db2098f3cb1c6ea8173d83 (MD5) Previous issue date: 2020

The lack of human resources and the damage that weeds can cause to coffee plants are the causes of the growing demand for selective herbicides to be used in coffee farming. Thus, the objective of this study was to evaluate the phytotoxicity symptoms and leaf anatomical characteristics of young coffee plants submitted to application isolated herbicides and also in associations. An experiment was conducted in a protected environment in randomized blocks: four replications with coffee seedlings (Coffea arabica L.) cultivar “Topazio MG-1190”, grown in pots with a capacity of 11 liters of substrate. The herbicides applied, in isolation, were: pyrazosulfuron-ethyl (15 g ha-1), saflufenacil (49 g ha-1), imazetaphyr (100 g ha-1), iodosulfuron-methyl (3.5 g ha-1), chlorimuron-ethyl (15 g ha-1) and sethoxydim (184 g ha-1). The latter was used in associations with the others. In addition, a control without herbicides was used. Phytotoxicity symptoms were evaluated up to 49 days after application (DAA) and anatomical characteristics at 65 DAA. Saflufenacil alone and in association with sethoxydim caused visual phytotoxicity symptoms in the leaves and negatively influence in the characteristics of the epidermis thickness of the adaxial face (EAD), thickness of the palisade parenchyma (EPP), thickness of the spongy parenchyma (EPE) and thickness of the mesophyll (MES). The other herbicides, isolated or in associations, didn’t cause phytotoxicity symptoms, but had a negative influence in the anatomical parameters of the leaf blade, however they did not interfere with the paradermic parameters and the vascular bundle. Key words: Coffea arabica; leaf; selectivity; symptoms.

The aim of this study was to investigate the effects of coffee species, roast degree and decaffeination on in vitro probiotic bacterial growth, and to identify the major coffee compounds responsible for such effects. Six C. arabica and C. canephora extracts (regular medium and dark roasted and decaffeinated medium roasted), and five bioactive compounds (chlorogenic acid, galactomannan, type 2 arabinogalactan, caffeine and trigonelline) were individually incorporated into a modified low-carbon broth medium-(mMRS), at different concentrations (0.5 to 1.5% soluble coffee and 0.05 to 0.8 mg mL-1 standard solutions). Inulin and fructooligosaccharides (FOS) were used as prebiotic references. MRS and mMRS were used as rich and poor medium controls, respectively. The growth of Lactobacillus rhamnosus GG ATCC 53103-(GG), L. acidophilus LA-5-(LA), Bifidobacterium animalis DN-173010-(BA) and B. animalis subsp. lactis BB12-(BB12), as well as the growth inhibition of non-probiotic Escherichia coli ATCC 25922 were evaluated. Differences in growth between mMRS and treatments (Δlog CFU mL-1) were compared by ANOVA and Tukey's test, and considered when p ≤ 0.05. Overall, after 48 h incubation, the medium roasted arabica coffee extract increased the growth of GG, LA and BA (range: Δlog CFU mL-1 = 0.5 to 1.8), while the dark roasted arabica coffee extract increased BB12 growth (range: Δlog CFU mL-1 = 0.9 to 1.7), in a dose dependent manner. Improved performances of GG, LA and BA were promoted by higher polysaccharides and CGA concentrations, with better performance for Lactobacillus sp. The tested coffee bioactive compounds promoted the poor growth of BB12. Plain caffeine did not promote Bifidobacterium sp. growth and limited the growth of Lactobacillus sp. Regular C. arabica and C. canephora extracts inhibited the growth of E. coli, while the decaffeinated extracts promoted its growth. The present results show that coffee consumption can selectively improve the growth of probiotic strains, thus exerting a prebiotic effect, and show that coffee roasting and decaffeination affect this property and that different strains utilize different coffee components to grow.

Phosphate (P) bioavailability is severely constrained in volcanic ash soils due to its high fixation rate. To overcome this problem the use of P-solubilizing fungi (PSF) has been proposed gaining recently great attention. However, despite encouraging results in the laboratory, their effectiveness under field conditions is debatable. To provide a better understanding of the dynamics of PSF in soils and to establish criteria for screening effective PSF a series of studies were conducted. PSF were isolated from coffee plantations grown in a Typic Udivitrand (QU), a Pachic Fulvundand (CH), and a Typic Melanudand (Ti). Fifty-four isolates (27 from CH, 19 from Ti, and 8 from QU) produced index of P solubilization among 16 and 106 mg L-1 using as P source phosphate rock. The results suggest that the microbial P solubilization was not only associated to the decrease in the culture medium pH, but also the production of organic acids associated with the most effective PSF. The higher production of organic acids seems to be associated lower fungal colony growth rate likely due to a carbon/energy drainage. The soil P-fixation capacity, soil organic content and degree of humification seems to control the relative abundance of PSF in the soils tested. In soils cultivated with coffee Phlebia subserialis is reported, for the first time, as a PSF. Key words: Colombian coffee zone; soil fertility; phosphorus fixation; organic acids; humificaction degree; Phlebia.

Glyphosate is the most used product for weed control in young coffee planting rows. The application is carried out in a directed way, avoiding the contact of the product with the crop. However, drift and consequent plant injuries frequently occur. An alternative to reduce these injuries is the use of attenuators capable of reducing the negative effects caused by this herbicide. Therefore, the objective of this study is to verify the efficiency of foliar fertilizers (Fertiactyl Pos® and Crop+) as attenuators of the toxic effects caused by the use of glyphosate in the coffee crop. Thus, coffee seedlings were subjected to different doses of the mixture of Fertiactyl Pos® with glyphosate and Crop+ with glyphosate. The treatments were applied on top of the plants. The percentage of coffee intoxication was evaluated at 14, 21, 28, 35, 42 and 70 days after application. At 90 days after application, height, leaf area and shoot and root dry matter were evaluated. Higher doses of glyphosate (1440 and 2880 g ha-1) without the use of attenuators caused intoxication levels close to 80% at 28 and 90 DAA. The use of Fertiactyl Pos® and Crop+ reduced the injuries caused by the application of glyphosate, regardless of the dose of herbicide and fertilizers, but with different levels of action. All the morphological variables measured suffered a negative action with the isolated use of glyphosate, with a tendency to recover when the attenuators were applied. It is concluded that the two attenuators were efficient in suppressing the deleterious effects of glyphosate on coffee plants affected by this herbicide. However, the attenuator Crop+ showed better recovery results. For 720 g ha-1 of glyphosate, the dose most used in the field, 8 L ha-1 is recommended for Fertiactyl Pos® and 4 L ha-1 for Crop+. Key words: Coffea arabica; Crop+; Fertiactyl Pos®; Injury; Weed control.

Coffee, besides being one of the most consumed stimulating beverages in the world, has important bioactive activities, which have been attracting increasing attention from researchers. However, the standard process of roasting causes changes in its chemical composition. In the present study, extracts obtained from green and roasted beans (light, medium and dark) of Coffea arabica Linnaeus were submitted to high-power ultrasonic extraction and atomization by spray drying. Colorimetric analysis was used to classify the roasting levels of the dried extract samples. The effects of the roasting process on the bioactivity of the dried extracts were verified through the following assays: caffeine, chlorogenic acid and caffeic acid, by HPLC-PDA; total phenolics by Folin–Ciocalteu; antioxidant activity by DPPH, FRAP, ABTS and ORAC; antiproliferative activity, using the MTT assay; and cell cycle and apoptosis by flow cytometry in metastatic prostate cancer cell lines from bone (PC-3) and brain (DU-145). The results showed that the lowest levels of caffeine, chlorogenic and caffeic acids were observed in dark roasted coffee. In comparison to medium and dark extracts in PC-3 cells, the green and light coffee extracts had higher antioxidant activities and promoted cytotoxicity followed by cell cycle arrest in phase S and apoptosis induction. Thus, the roasting level of the coffee extracts may be related to the potential chemoprotective effects of Coffea arabica L. in prostate cancer cells.

Detection of spatial variability of data that can improve crop management is a key factor for precision agriculture. In agriculture, there is a need for tools to assist farmers in decision-making about proper nutrient management, aiming to achieve their full productive potential. Based on that, this study aimed to (1) determine the spatial correlations between the chlorophyll index (CI) and the foliar levels of nitrogen, phosphorus and potassium (NPK) in the coffee crop using geostatistical tools; and (2) to evaluate the potential use of this index as a tool for site-specific nutrient management in an irrigated coffee field. For that, a study was carried out in a 2.1 ha area under arabica coffee cultivation in Paula Cândido, Minas Gerais State, Brazil. Samplings of the CI were performed in 1141 plants using a portable chlorophyll meter (SPAD-502). Regarding the NPK analysis, leaf samples from one of each 10 plants used to measure the CI were taken for chemical analysis (114 plants). Then, the data were submitted to descriptive and geostatistical analysis. For the spatial correlation analysis, the Moran Bivariate Global (I) and the Local index (Ixy) were used. The results showed a moderate correlation between the CI and N (0.500), showing the potential of the chlorophyll meter as a tool for site-specific nitrogen management in the coffee crop. Differently, the CI is not recommended for P and K management since they were not well correlated. Lastly, as a tool that performs indirect measurements, the results from the chlorophyll meter should be validated by field measurements to local calibrations.

Oil recovery, retention index, and thermodynamic parameters of green coffee beans (GCB) and its press cake (PC) extraction using bio-based solvents were investigated. The extraction parameters investigated were temperature (35 to 55 °C), type of material (coffee beans and press cake), and type of solvent (ethanol, acetone, and ethyl acetate), at a fixed solvent to solid mass ratio (5:1) (w/w). The fatty acid profile of the ethanolic extract was assessed for both GCB and PC, and compared to the oil obtained from the mechanical pressing. It was observed that higher temperatures affected positively the extraction yields, especially when acetone and ethanol were employed, allowing a recovery up to 90% and 56.7% for GCB and PC, respectively. The solution retained in the raffinate phase from the GCB extraction was greater than that for the PC. For all operational levels, the ∆H and ∆S were positive. ∆G decreased with increasing temperature. Palmitic and linoleic acids were predominant in all types of oil. The oil obtained by pressing showed higher content of linoleic acid (45.32%), while the solvent-extracted oil from GCB had more palmitic acid (34.79%), and the PC oil presented intermediate levels of all the methyl esters.

Coffee is one of the main economic crops in the world and is now widely grown throughout Taiwan. The process of roasting coffee begins with the heating and smooth expansion of raw beans, which leads to changes in appearance and color while affecting the flavor and taste of coffee. So far, most coffee manufacturers have used visual inspection or colorimeter methods to identify differences in coffee quality. Moreover, there is no literature discussing the correlation of roasted bean color with caffeine and chlorogenic acid content. Therefore, the purpose of this experiment was to analyze the chlorogenic acid and caffeine content and their correlation with bean color under different roasting degrees and from different sources to establish basic data for the rapid identification of coffee quality in the future. In this experiment, the coffee Coffea arabica typica from Dongshan, Gukeng, and Sumatra’s Indonesian rainforest was used, and the beans were roasted into four degrees: raw bean, light, medium, and dark roast, to investigate the appearance of the coffee beans and its correlation with caffeine and chlorogenic acid content. The results showed that with a higher roasting degree, caffeine content increased gradually, except for Indonesian beans, but the chlorogenic acid content in all samples showed a declining trend with the increase in roasting degree. The correlation between the chlorogenic acid content and the color space value of the coffee bean color shows that L*, a*, and h° in both ground and unground coffee are highly correlated. The C* value of the ground and unground coffee showed a correlation coefficient of r = 0.159 ns and 0.299 ns, respectively. The correlation between the caffeine content and the color space value of the unground coffee bean shows that the a*, b*, and C* value is highly correlated with the caffeine content. The color space values of ground coffee beans show no correlation with caffeine.

Epidemiological studies have shown an inverse association between coffee consumption and the development of Parkinson’s disease (PD). The effects of the oral treatment with green (non-roasted) coffee extracts (CE, 100 or 400 mg/kg) and caffeine (31.2 mg/kg) were evaluated on catalepsy induced by haloperidol in mice, and unilateral 6-OHDA lesion of medial forebrain bundle (MFB) or striatum in rats. Also, the in vitro antioxidant activity and the monoamine levels in the striatum were investigated. CE presented a mild antioxidant activity in vitro and its administration decreased the catalepsy index. CE at the dose of 400 mg/kg induced ipsilateral rotations 14 days after lesion; however, chronic 30-day CE and caffeine treatments did not interfere with the animals’ rotation after apomorphine or methamphetamine challenges in animals with MFB lesion, nor on monoamines levels. Furthermore, CE and caffeine were effective in inhibiting the asymmetry between ipsilateral and contralateral rotations induced by methamphetamine and apomorphine in animals with lesion in the striatum but did not avoid the monoamines depletion. These results indicate that CE components indirectly modulate dopaminergic transmission, suggesting a pro-dopaminergic action of CE, and further investigation must be conducted to elucidate the mechanisms of action and the possible neuroprotective role in PD.

The community health plans commonly use malathion (MAL), an organophosphate pesticide (OP), to eliminate pathogenic insects. The objective of the present research is to evaluate the consequences of Coffea arabica L. oil and Olea europaea L. oil on MAL-intoxicated male rats. Six equal groups of animals were used for conducting this study (n = 10). Animals in group one were designated as control, animals belonging to group two were exposed to MAL in the measure of hundred mg per kg BW (body weight) for forty-nine days (seven weeks), rats in the third and fourth groups were administered with 400 mg/kg BW of Coffea arabica L. and Olea europaea L. oils, respectively, and the same amount of MAL as given to the second group. Groups five and six were administered with the same amount of Coffea arabica L. oil and Olea europaea L. oil as given to group three. Exposure of rats to 100 mg/kg body weight of MAL resulted in statistical alteration of the serum lipid profile. A marked decline was noticed in the severe changes of these blood parameters when MAL-intoxicated rats were treated with Coffea arabica L. oil and Olea europaea L. oil. Two compounds from Coffea arabica L. oil (Chlorogenic acid) and Olea europaea L. oil (Oleuropein) demonstrated good interaction with xanthine oxidase (XO) and 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR) enzymes that are associated with cholesterol production. The present study indicated that Coffea arabica L. oil and Olea europaea L. oil could be considered prospective and potential healing agents against metabolic conditions induced by MAL.

Made available in DSpace on 2022-04-28T19:47:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-25 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Group of Genomics and Transcriptomes in Plants Department of Biodiversity Institute of Biosciences UNESP São Paulo State University Department of Agronomy UEL State University of Londrina Group of Genomics and Transcriptomes in Plants Department of Biodiversity Institute of Biosciences UNESP São Paulo State University

Resumen: El objetivo de esta investigación fue caracterizar los compuestos fenólicos y actividad antioxidante de la pulpa de café (Coffea arabica L.) deshidratada a través de dos métodos de secado (aire caliente y liofilizado). Las muestras de pulpa de café se obtuvieron de tres provincias de la región Amazonas (Perú), para luego ser sometidas a un proceso de lavado, oreado, deshidratado, molienda y extracción utilizando agua ultra pura y cuatro solventes orgánicos: etanol 50 %, etanol 95 %, metanol 50 % y metanol 95 %. Se encontró que los extractos obtenidos mediante liofilización y solvente etanol al 95 % tienen la mayor capacidad de extracción, compuestos fenólicos y capacidad antioxidante, con 66.3 % y 402.6 mg equivalentes de ácido gálico/100 g pulpa deshidratada, respectivamente. Se concluye que el etanol al 95 % es el mejor solvente para la extracción de compuestos fenólicos en pulpa de café deshidratada por liofilización.

Plant breeding aims to develop cultivars with good agronomic traits through gene recombination and elite genotype selection. To support Coffea arabica breeding programs and assist parent selection, molecular characterization, genetic diversity (GD) analyses, and circulating diallel studies were strategically integrated to develop new cultivars. Molecular markers were used to assess the GD of 76 candidate parents and verify the crossing of potential F1 hybrids. Based on the complementary agronomic traits and genetic distance, eight elite parents were selected for circulating diallel analysis. The parents and 12 hybrids were evaluated based on 10 morpho-agronomic traits. For each trait, the effects of general and specific combining abilities, as well as the averages of the parents, hybrids, and predicted hybrids, were estimated. Crosses that maximize the genetic gains for the main agronomic traits of C. arabica were identified. Joint analysis of phenotypic and molecular data was used to estimate the correlation between molecular GD, phenotypic diversity (PD), phenotypic mean, and combining ability. The selection of parents that optimize the allele combination for the important traits of C. arabica is discussed in detail. Made available in DSpace on 2022-01-27T01:58:47Z (GMT). No. of bitstreams: 1 Combining-Ability-and-Molecular.pdf: 2421339 bytes, checksum: b1fd8d047ff3e47af308c96d0c676428 (MD5) Previous issue date: 2021

Coffee production is one of the main agricultural activities in Brazil, and several coffee cultivars with disease resistance have already been developed. The secondary metabolites produced by plants are closely associated with defense strategies, and the resistance of coffee cultivars to bacterial halo blight (BHB) can be related to these compounds. Therefore, this study aims to compare a partially resistant coffee cultivar (Iapar-59) and a susceptible cultivar (Mundo Novo 376/4) to BHB (Pseudomonas syringae pv. garcae) in relation to the chemical composition and antioxidant activity of the leaf extracts. In addition, this study determined the total phenolic and flavonoid contents and phenolic profiles of the Iapar-59 leaf extracts of plants inoculated with P. syringae pv. garcae. The Iapar-59 extract showed a higher content of phenolic compounds and flavonoids than the Mundo Novo 376/4 extract. Both cultivars contained gallic, chlorogenic and caffeic acids; however, the highest contents were quantified in the Iapar-59 cultivar. The leaf extracts from the Iapar-59 cultivar exhibited higher antioxidant activity. Higher concentrations of gallic, caffeic and chlorogenic acids and the presence of vanillin were detected in the extract of cultivar Iapar-59 inoculated with P. syringae pv. garcae.

SummaryCoffee (Coffea arabicaL.) presents an asynchronous flowering regulated by endogenous and environmental stimulus, and anthesis occurs once plants are rehydrated after a period of water deficit.We evaluated the evolution of Abscisic Acid (ABA), ethylene, 1-aminocyclopropane-1-carboxylate (ACC) content, ACC oxidase (ACO) activity, and expression analysis of theLysine Histidine Transporter1 (LHT1)transporter, in roots, leaves and, flower buds from three coffee genotypes (Coffea arabicaL. cv Oeiras, Acauã, andSemperflorens) cultivated under field conditions with two experiments. In a third field experiment, the effect of exogenous supply of ACC in coffee anthesis was evaluated.We found an increased ACC level in all tissues from the three coffee genotypes in the re-watering period just before anthesis for all tissues and high expression of theLHT1gene in flower buds and leaves. Ethylene content and ACO activity decreased from rainy to dry period whereas ABA content increased. Higher number of opened and G6 stage flower buds were observed in the treatment with exogenous ACC.The results showed that the interaction of ABA-ACO-ethylene and intercellular ACC transport among leaves, buds, and roots in coffee favors an increased level of ACC that is most likely, involved as a modulator in coffee anthesis.