1. Evaluation and application of an efficient plant DNA extraction protocol for laboratory and field testing
- Author
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Wu Wei, Qi Wang, Lin Li, Xiao-Xia Shen, Qiu Tian, Huixia Shou, and Zhi'an Wang
- Subjects
0301 basic medicine ,DNA, Plant ,Loop-mediated isothermal amplification ,Polymerase Chain Reaction ,General Biochemistry, Genetics and Molecular Biology ,law.invention ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,law ,General Pharmacology, Toxicology and Pharmaceutics ,Cellulose ,Polymerase chain reaction ,Research Articles ,Chromatography ,General Veterinary ,Filter paper ,Chemistry ,General Medicine ,DNA extraction ,030104 developmental biology ,Molecular Diagnostic Techniques ,030220 oncology & carcinogenesis ,Nucleic acid ,Nucleic Acid Amplification Techniques ,DNA ,GC-content - Abstract
Nucleic acids in plant tissue lysates can be captured quickly by a cellulose filter paper and prepared for amplification after a quick purification. In this study, a published filter paper strip method was modified by sticking the filter paper on a polyvinyl chloride resin (PVC) sheet. This modified method is named EZ-D, for EASY DNA extraction. Compared with the original cetyl trimethylammonium bromide (CTAB) method, DNA extracted by EZ-D is more efficient in polymerase chain reaction (PCR) amplification due to the more stable performance of the EZ-D stick. The EZ-D method is also faster, easier, and cheaper. PCR analyses showed that DNA extracted from several types of plant tissues by EZ-D was appropriate for specific identification of biological samples. A regular PCR reaction can detect the EZ-D-extracted DNA template at concentration as low as 0.1 ng/μL. Evaluation of the EZ-D showed that DNA extracts could be successfully amplified by PCR reaction for DNA fragments up to 3000 bp in length and up to 80% in GC content. EZ-D was successfully used for DNA extraction from a variety of plant species and plant tissues. Moreover, when EZ-D was combined with the loop-mediated isothermal amplification (LAMP) method, DNA identification of biological samples could be achieved without the need for specialized equipment. As an optimized DNA purification method, EZ-D shows great advantages in application and can be used widely in laboratories where equipment is limited and rapid results are required.
- Published
- 2021