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The aim of this study is to compare analytical performance characteristics and also the patient results obtained from both Modified Jaffe’s kinetic and Enzymatic Trinder methods for serum creatinine so as to identify risk zone, if present, within the measurement range. Serum creatinine was measured on 206 left-over serum samples by Modified Jaffe’s kinetic and Enzymatic Trinder methods. For analytical performance comparisons, limit of detection(LOD), limit of quantification(LOQ), linearity, measuring range, intra and inter-assay CV were measured and compared. Statistical comparisons were done by Pearson‘s correlation coefficient and Bland-Altman tests. For Enzymatic Trinder and Modified Jaffe’s kinetic methods, LODs for serum creatinine were 0.01 & 0.02 mg/dl respectively; LOQs were 0.04 & 0.06 mg/dl respectively; linearity were upto 55 mg/dl & 30 mg/dl respectively. Correlation coefficient was high (r=0.99); intra and inter-assay CV measurements were acceptable. However, CV was lower for Enzymatic Trinder method. Bland-Altman plot showed that more than 95% data points lie within ± 1.96 SD limit of mean difference value (0.16). Average discrepancy (ie. bias) was 0.16 mg/dl across whole measurement range. However, at low concentrations, Modified Jaffe’s kinetic method gave higher values indicating systematic bias, thereby forming a “risk zone” in measurement range. Analytical performance requirements were met by both methods for routine use and good agreement exists between them. However, better performance was not shown by Modified Jaffe’s kinetic method at low concentrations. Such a “risk zone” needs to be identified by laboratories for accurate reporting of creatinine results.

Background: Polycystic ovary syndrome (PCOS) is also known as hyperandrogenic anovulation (HA) or Stein-Leventhal syndrome. PCOS has a diverse range of causes that are not entirely understood, but there is evidence that it is largely a genetic disease. Aim of the study was to evaluate better predictor of cardiovascular disease risk in women with polycystic ovary syndrome. Methods: 50 diagnosed patients of PCOS and 50 age matched healthy females were included in this study. Cases were diagnosed based on new Rotterdam criteria formulated by the American Society for Reproductive Medicine (ASRM). Fasting Blood samples were collected. Serum High sensitivity-C reactive protein (hs-CRP) and Malondialdehyde (MDA) were estimated. Results: Results were analyzed using unpaired t-test and p-value was calculated. Statistically non-significant increased levels of serum hs-CRP in PCOS cases as compared with controls were observed. MDA was found to be significantly increased in cases as compared to controls. ROC curve analysis shows MDA as a more specific predictor of cardiovascular disease risk in PCOS compared to hs-CRP. Conclusions: Increase in the serum MDA level indicates increased formation of reactive oxygen species and lipid peroxidation which leads to increased oxidative stress and this may increase cardiovascular disease risk in PCOS. hs-CRP a marker of chronic inflammation was not significantly increased in PCOS.

Background: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder affecting women of reproductive age group with a worldwide prevalence of about 4 to12%1,2. PCOS is seen in up to 25% of the Caucasians and 50% of the South Asian women respectively3,4. It is a heterogeneous disorder of unknown aetiology with a strong genetic element5. PCOS does not exclusively involve the reproductive apparatus; it has a complex number of systemic relevancy symptoms. It leads to Metabolic Syndrome with severe consequences on the cardiovascular system6. The precise cardiovascular disease (CVD) risk in women with PCOS remains unclear because there are no longitudinal studies pertaining to cardiovascular events. There is a paucity of data in regard to cardiovascular event rates and mortality in PCOS hence the present study was aimed to assess cardiovascular disease risk in women with PCOS. Aim: To evaluate cardiovascular disease risk in women with Polycystic ovary syndrome. Materials and Methods: 50 diagnosed cases of PCOS and 50 age matched healthy females were included in this study. Cases were diagnosed based on new Rotterdam criteria formulated by the American Society for Reproductive Medicine (ASRM) and the European Society for Human Reproduction and Embryology (ESHRE). Blood samples were collected after overnight fasting. Serum High sensitivity-C Reactive Protein (hs-CRP), Total Cholesterol(TC), Triglycerides(TG), HDL Cholesterol(HDL-C), VLDL Cholesterol(VLDL-C), LDL Cholesterol (LDL-C) were estimated. Body Mass Index (BMI) was calculated for women in both the groups. Statistical analysis: Results were analyzed using unpaired t-test and p-value was calculated. BMI was correlated with hs-CRP using one way ANOVA test. Statistically non-significant increased levels of serum total cholesterol, Triglycerides, HDL cholesterol, LDL cholesterol, VLDL cholesterol and hs-CRP in PCOS cases as compared with control were observed. BMI was found to be significantly inc

Suicidal tendencies are one of the major social problems worldwide. It is equally seen in Indian population. Organo-phosphorus compounds are usually preferred because of their easy availability. Paraquat is one of the lesser known herbicide agent, which is not an OP compound. It is highly toxic and causes damage to the lungs, liver and kidneys. Occurrence of paraquat poisoning is rare, but has very high mortality rate. Here we report one such case which was earlier misdiagnosed as organo-phosphorus poisoning, but diagnosed later by qualitative urine sodium dithionite test. It is a simple test for early diagnosis of paraquat poisoning. It is recommended to performing this test in all suspected cases of oral poisoning.