1. Highly selective monoclonal antibody-based lateral flow immunoassay for visual and sensitive determination of conazole fungicides propiconazole in vegetables
- Author
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Hongtao Lei, Jing-Nan Meng, Lei Yi, Xing Shen, Liu Haihong, Ze-Ke Xu, Xing-Xing Yang, Wang Tingcai, Yi-Yong Yan, and Zhen-Lin Xu
- Subjects
medicine.drug_class ,Health, Toxicology and Mutagenesis ,Gold Colloid ,Toxicology ,Monoclonal antibody ,Rapid detection ,chemistry.chemical_compound ,Limit of Detection ,Tandem Mass Spectrometry ,Vegetables ,medicine ,Animals ,Chromatography, High Pressure Liquid ,Immunoassay ,Mice, Inbred BALB C ,Chromatography ,Public Health, Environmental and Occupational Health ,Antibodies, Monoclonal ,General Chemistry ,General Medicine ,Triazoles ,Highly selective ,Fungicides, Industrial ,Propiconazole ,Fungicide ,Kinetics ,chemistry ,Haptens ,Food Science ,Lateral flow immunoassay - Abstract
The conazole fungicide propiconazole is frequently found in vegetables although usage is not allowed. To overcome the high-cost and time-consuming labour requirements of instrumental methods, we developed a simple and visual lateral flow immunoassay for the sensitive determination of propiconazole. A hapten was carefully designed to raise a monoclonal antibody against propiconazole. Bal b/c mice were immunised with the hapten-carrier protein conjugate and a specific monoclonal antibody (mAb) was produced. Based on this mAb, a sensitive immunochromatographic strip assay (ICA) was established for rapid screening of propiconazole in vegetable samples. After optimisation of analytical parameters, the ICA strip showed a detection limit of 0.13 ng g���1 and a linear range from 0.5 to 80 ng g���1 using a strip reader. The assay also can be read by the naked eye with a visual limit of detection of 80 ng g���1. The recoveries for spiked vegetable samples by ICA ranged from 85.2% to 114.9%, with a coefficient of variation less than 11.7%. The assay time is within 45 min for a single sample including the sample pre-treatment. For spiked and blind samples, the detection capability of ICA was equivalent to liquid chromatography-mass spectrometry.
- Published
- 2021