10 results on '"WEI, Feng"'
Search Results
2. Identification of Daphne genkwa and Its Vinegar-Processed Products by Ultraperformance Liquid Chromatography–Quadrupole Time-of-Flight Mass Spectrometry and Chemometrics.
- Author
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Mi, Hongying, Zhang, Ping, Yao, Lingwen, Gao, Huiyuan, Wei, Feng, Lu, Tulin, and Ma, Shuangcheng
- Subjects
CHEMOMETRICS ,TIME-of-flight mass spectrometry ,MULTIVARIATE analysis ,CHINESE medicine ,CAFFEIC acid ,MASS spectrometry ,QUADRUPOLE ion trap mass spectrometry - Abstract
Crude herbs of Daphne genkwa (CHDG) are often used in traditional Chinese medicine to treat scabies baldness, carbuncles, and chilblain owing to their significant purgation and curative effects. The most common technique for processing DG involves the use of vinegar to reduce the toxicity of CHDG and enhance its clinical efficacy. Vinegar-processed DG (VPDG) is used as an internal medicine to treat chest and abdominal water accumulation, phlegm accumulation, asthma, and constipation, among other diseases. In this study, the changes in the chemical composition of CHDG after vinegar processing and the inner components of the changed curative effects were elucidated using optimized ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Untargeted metabolomics, based on multivariate statistical analyses, was also used to profile differences between CHDG and VPDG. Eight marker compounds were identified using orthogonal partial least-squares discrimination analysis, which indicated significant differences between CHDG and VPDG. The concentrations of apigenin-7-O-β-d-methylglucuronate and hydroxygenkwanin were considerably higher in VPDG than those in CHDG, whereas the amounts of caffeic acid, quercetin, tiliroside, naringenin, genkwanines O, and orthobenzoate 2 were significantly lower. The obtained results can indicate the transformation mechanisms of certain changed compounds. To the best of our knowledge, this study is the first to employ mass spectrometry to detect the marker components of CHDG and VPDG. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
3. Chemical profiling and quantification of monacolins and citrinin in red yeast rice commercial raw materials and dietary supplements using liquid chromatography-accurate QToF mass spectrometry: Chemometrics application
- Author
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S Sagi, Mei Wang, Bharathi Avula, Jerry Zweigenbaum, Pieter A. Cohen, Ma Shuangcheng, Yan-Hong Wang, Wei Feng, and Ikhlas A. Khan
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Quality Control ,inorganic chemicals ,Clinical Biochemistry ,Pharmaceutical Science ,Mass spectrometry ,Mass Spectrometry ,Analytical Chemistry ,Chemometrics ,Pigment ,chemistry.chemical_compound ,Drug Discovery ,Red yeast rice ,Lovastatin ,Chromatography, High Pressure Liquid ,Spectroscopy ,Biological Products ,Chromatography, Reverse-Phase ,Principal Component Analysis ,Chromatography ,biology ,Discriminant Analysis ,Reproducibility of Results ,Reference Standards ,musculoskeletal system ,Monascus ,biology.organism_classification ,Yeast ,Citrinin ,chemistry ,visual_art ,Calibration ,Dietary Supplements ,Linear Models ,cardiovascular system ,visual_art.visual_art_medium ,Fermentation ,Azo Compounds ,tissues - Abstract
Red yeast rice (RYR) is prepared by fermenting rice with various strains of the yeast Monascus spp of the Aspergillaceae family. Depending on the Monascus strains and the fermentation conditions, the products may contain monacolins, pigments and citrinin as secondary metabolites. Authentic and commercial RYR samples were analyzed using UHPLC-DAD-QToF-MS for monacolins, pigments and citrinin. A separation by UHPLC was achieved using a reversed-phase column and a gradient of water/acetonitrile each containing formic acid as the mobile phase. Accurate mass QToF spectrometry was used to distinguish isobaric monacolins. Principle component analysis (PCA), a chemometric technique was used to discriminate between authentic RYR, commercial RYR raw materials and dietary supplements. Three authentic RYR samples, 31 commercial RYR raw materials and 14 RYR dietary supplements were analyzed. Monacolin K content in 600mg of authentic RYR samples ranged from 1.2mg to 1.38mg. Amounts of monacolin K in dietary supplements labeled as containing 600mg of RYR varied more than 40-fold from 0.03mg to 2.18mg. Monacolin K content of dietary supplements labeled as containing 1200mg RYR varied more than 20-fold from 0.22mg to 5.23mg. In addition to large variations in quantity of monacolin K found in dietary supplements, RYR dietary supplements contained ratios of monacolins that differed significantly from authentic samples. The results indicated that RYR commercial products are of variable quality and the analytical method is suitable for quality control testing of a variety of RYR products.
- Published
- 2014
4. Development of the chromatographic fingerprint of Scutellaria barbata D. Don by GC–MS combined with Chemometrics methods
- Author
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Wei-wei Feng, Hongmei Lu, Ruijing Pan, Fang-Qiu Guo, and Yi-Zeng Liang
- Subjects
Quality Control ,China ,Correlation coefficient ,Scutellaria ,Clinical Biochemistry ,Analytical chemistry ,Pharmaceutical Science ,Pharmacognosy ,Gas Chromatography-Mass Spectrometry ,Analytical Chemistry ,law.invention ,Oldenlandia ,Chemometrics ,law ,Drug Discovery ,Lobelia chinensis ,Oils, Volatile ,Plant Oils ,Spectroscopy ,Essential oil ,Lobelia ,Principal Component Analysis ,Chromatography ,biology ,Chemistry ,Reproducibility of Results ,biology.organism_classification ,Gas chromatography ,Gas chromatography–mass spectrometry ,Scutellaria barbata ,Drugs, Chinese Herbal - Abstract
Gas chromatography fingerprint of Scutellaria barbata D. Don (SB) from different origins was studied by gas chromatography-mass spectrometry (GC-MS) and related Chemometrics methods. The constituents of essential oil of Scutellaria barbata D. Don (SB) and its two adulterants Oldenlandia diffusa (OD) and Lobelia chinensis Lour (LCL) were analyzed and compared, 50, 36 and 38 components were identified from SB, OD and LCL, respectively, there were 16 and 18 common components between SB and OD, SB and LCL. Nine different samples collected from different producing areas of SB were studied. The relative standard deviations (RSDs) of retention time and peak area of each component were less than 0.2% and 6%, respectively. The number of common peaks was up to 52 and the un-common peak area was less than 10%. The similarity and difference among SB and its adulterants were also evaluated by correlation coefficient similarity analysis and principal component analysis, the result showed that developed fingerprint characterize the SB from different producing areas and it is useful and feasible for the discrimination of its adulterants.
- Published
- 2011
5. Optimum extraction process of polyphenols from the bark of Phyllanthus emblica L. based on the response surface methodology
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Jian Chen, Li Yang, Liang Zhu, Wei-Feng Li, Jian-Guo Jiang, and Ding-Yong Wang
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Flavonoids ,Chromatography ,Models, Statistical ,Ethanol ,Chemistry ,Extraction (chemistry) ,Temperature ,Polyphenols ,Filtration and Separation ,Phyllanthus emblica ,Analytical Chemistry ,Solvent ,Chemometrics ,Kinetics ,Phenols ,Polyphenol ,visual_art ,visual_art.visual_art_medium ,Plant Bark ,Bark ,Sample preparation ,Response surface methodology - Abstract
Phyllanthus emblica L. is an economic plant used in Chinese medicine for the treatment of various diseases. The bark of P. emblica is rich in polyphenols and its extractions have shown strong antioxidative and radical scavenging activity. Response surface methodology (RSM) was used to assess the optimal extraction of polyphenols from P. emblica bark. Various extraction parameters including ethanol concentration, extraction time, temperature, solid-liquid ratio, and extraction times were chosen to identify their effects on polyphenols extraction. Among these parameters, extraction times and solvent concentration were found to have significant effect on polyphenols extraction. RSM was applied to obtain the optimal combination of solvent concentration, extraction time, temperature, and extraction time for maximum rate of extraction. The most suitable condition for the extraction of polyphenols was at ethanol concentration 75%, extraction time 25 min, extraction temperature 45 degrees C, and extraction times 3. At these optimal extraction parameters, the maximum extraction of polyphenols obtained experimentally was found to be very close to its predicted value. The extraction rate of polyphenols was 19.78% at the optimum conditions. The mathematical model developed was found to fit with the experimental data of polyphenols extraction.
- Published
- 2009
6. Identification of Ophiocordyceps sinensis and Its Artificially Cultured Ophiocordyceps Mycelia by Ultra-Performance Liquid Chromatography/Orbitrap Fusion Mass Spectrometry and Chemometrics.
- Author
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Zhang, Ping, Li, Saina, Li, Juan, Wei, Feng, Cheng, Xianlong, Zhang, Guifeng, Ma, Shuangcheng, and Liu, Bin
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OPHIOCORDYCIPITACEAE ,LIQUID chromatography ,CHINESE medicine ,AMINO acids ,PEPTIDES - Abstract
Since the cost of
Ophiocordyceps sinensis , an important fungal drug used in Chinese medicine, has increased dramatically, and the counterfeits may have adverse health effects, a rapid and precise marker using the peptide mass spectrometry identification system could significantly enhance the regulatory capacity. In this study, we determined the marker peptides in the digested mixtures of fungal proteins in wildO. sinensis fruiting bodies and various commercially available mycelium fermented powders using ultra-performance liquid chromatography/Orbitrap Fusion mass spectrometry coupled with chemometrics. The results indicated the following marker peptides: TLLEAIDSIEPPK (m /z 713.39) was identified in the wildO. sinensis fruiting body, AVLSDAITLVR (m /z 579.34) was detected in the fermentedO. sinensis mycelium powder, FAELLEK (m /z 849.47) was found in the fermentedOphiocordyceps mycelium powder, LESVVTSFTK (m /z 555.80) was discovered in the artificialOphiocordyceps mycelium powder, and VPSSAVLR (m /z 414.75) was observed inO. mortierella mycelium powder. In order to verify the specificity and applicability of the method, the five marker peptides were synthesized and tested on all samples. All in all, to the best of our knowledge, this is the first time that mass spectrometry has been employed to detect the marker peptides ofO.sinensis and its related products. [ABSTRACT FROM AUTHOR]- Published
- 2018
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7. Analysis of the fingerprint profile of bioactive constituents of traditional Chinese medicinal materials derived from animal bile using the HPLC–ELSD and chemometric methods: An application of a reference scaleplate.
- Author
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Xiong, Jing, Zheng, Tian-jiao, Shi, Yan, Wei, Feng, Ma, Shuang-cheng, He, Lan, Wang, Si-cen, and Liu, Xin-she
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CHEMOMETRICS , *BILE , *HIERARCHICAL clustering (Cluster analysis) , *BILE acids , *SUPPORT vector machines , *SWINE - Abstract
• Bile acids are the major bioactive constituents of animal bile. • We established a fingerprint chromatogram method using HPLC coupled with ELSD. • We successfully implemented reference scaleplate for peak alignment. • Seventeen bile acids can be detected simultaneously using the method. Traditional Chinese medicinal materials derived from animal bile are widely applied in clinical therapy for thousands of years in several Southeast Asian countries. Although the constituents are similar, these crude drugs exhibit different pharmacological activities; bile acids are the main bioactive constituent. Depending on the source, the price of these crude drugs differs significantly. Therefore, a reliable fingerprint method is needed to analyze and distinguish these crude drugs with a similar composition. In this milieu, we aimed to establish a fingerprint chromatography method that can separate and detect several bile acids simultaneously. A high-performance liquid chromatography separation method was established with evaporative light scattering detection to detect the analytes. The main bioactive constituents of pig bile, cattle bile, sheep bile, bear bile, and three types of cow bezoar were analyzed using the proposed method. The fingerprint chromatography profile of 35 samples were obtained and analyzed using chemometric methods. Considering the differences among samples, a reference scaleplate method was used in the peak alignment procedure. Unsupervised methods (hierarchical cluster analysis and principle component analysis) and supervised methods (K-nearest neighbor, partial least squares discriminant analysis, support vector machine discriminant analysis, and soft independent modeling of class analogy) were used in the chemometric analysis. The results indicated that the fingerprint chromatograms of the seven crude drugs had fingerprint specificity and that they can be well distinguished. In addition, the reference scaleplate method using the chromatogram of a mixed standard solution is practically applicable for peak alignment in the analysis of samples with a large difference in chromatographic peaks. Overall, 17 bile acids can be separated and detected simultaneously using this method and some frequently used TCMMs derived from animal bile can be distinguished accurately. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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8. Simultaneous quantification of the major bile acids in Artificial Calculus bovis by high-performance liquid chromatography with precolumn derivatization and its application in quality control.
- Author
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Shi, Yan, Xiong, Jing, Sun, Dongmei, Liu, Wei, Wei, Feng, Ma, Shuangcheng, and Lin, Ruichao
- Subjects
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GALLSTONES , *BILE acids , *CHEMOMETRICS , *HIGH performance liquid chromatography , *DERIVATIZATION - Abstract
An accurate and sensitive high-performance liquid chromatography method coupled with ultralviolet detection and precolumn derivatization was developed for the simultaneous quantification of the major bile acids in Artificial Calculus bovis, including cholic acid, hyodeoxycholic acid, chenodeoxycholic acid, and deoxycholic acid. The extraction, derivatization, chromatographic separation, and detection parameters were fully optimized. The samples were extracted with methanol by ultrasonic extraction. Then, 2-bromine-4'-nitroacetophenone and 18-crown ether-6 were used for derivatization. The chromatographic separation was performed on an Agilent SB-C18 column (250 × 4.6 mm id, 5 μm) at a column temperature of 30°C and liquid flow rate of 1.0 mL/min using water and methanol as the mobile phase with a gradient elution. The detection wavelength was 263 nm. The method was extensively validated by evaluating the linearity ( r2 ≥ 0.9980), recovery (94.24-98.91%), limits of detection (0.25-0.31 ng) and limits of quantification (0.83-1.02 ng). Seventeen samples were analyzed using the developed and validated method. Then, the amounts of bile acids were analyzed by hierarchical agglomerative clustering analysis and principal component analysis. The results of the chemometric analysis showed that the contents of these compounds reflect the intrinsic quality of artificial Calculus bovis, and two compounds (hyodeoxycholic acid and chenodeoxycholic acid) were the most important markers for quality evaluating. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
9. A general procedure for establishing composite quality evaluation indices based on key quality attributes of traditional Chinese medicine.
- Author
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Chen, Jia, Cheng, Xian-Long, Li, Lin-Fu, Dai, Sheng-Yun, Wang, Ya-Dan, Li, Ming-Hua, Guo, Xiao-Han, Wei, Feng, and Ma, Shuang-Cheng
- Subjects
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CHINESE medicine , *QUADRUPOLE ion trap mass spectrometry , *QUADRUPOLE mass analyzers , *FLAVOR , *TANDEM mass spectrometry , *TIME-of-flight mass spectrometry , *HIGH performance liquid chromatography , *HERBAL medicine - Abstract
• Licorice roots are widely used in traditional Chinese medicine (TCM). • Quality attributesinTCMinclude growth years, location, and harvest season. • 11 licorice compounds are related tokey quality attributes of TCM formulations. • Liquiritin and 8 other compoundsare quality evaluation indices of licorice. • TCM composite quality evaluation indices will be used for quality control. Licorice, a medicinal herb and food flavor ingredient, has been widely used in traditional Chinese medicine (TCM) for the past 4000 years. In this study, we propose a new quality evaluation approach for licorice quality control based on the key quality attributes commonly used in TCM. The high quality of TCM formulations is ensured by verifying the genuine origin and implementing good agricultural and collection practices for each medicinal herb. In our study, the genuine production area, the harvest season, and the number of growth years were considered the key quality attributes of TCM. To ensure the representativeness of our analysis, we obtained a total of 158 licorice sample batches that differed in the number of growth years, the location of the production areas, and the season for harvesting. Initially, the 158 sample batches were subjected to ultra-high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS/MS). A preliminary screen identified 11 licorice compounds related to the three key quality attributes of TCM. An analysis by ultra-high-performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UHPLC-TQ-MS/MS) verified the presence of 34 compounds in all licorice samples. These 34 compounds included the 11 compounds related to the three key quality attributes of the samples, along with other bioactive components identified in previous studies. After using UHPLC-TQ-MS/MS to assess the signal peak intensities of the 34 compounds, we selected 17 licorice compounds to establish sample content evaluation indices, which were determined by high-performance liquid chromatography at four different wavelengths in all 158 licorice sample batches. Finally, the screen identified nine compounds that were closely associated with the quality attributes of licorice based on principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). Our results suggested that liquiritin and eight other compounds could be used as quality control indicators of licorice, which provided a foundation to establish the TCM quality composite evaluation index (TCM QCEI). In summary, this research concept can serve as a reference for research on quality markers and the evaluation of TCM. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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10. Rapid discrimination of Chinese red ginseng and Korean ginseng using an electronic nose coupled with chemometrics
- Author
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Li, Shan, Li, Xiang-ri, Wang, Gang-li, Nie, Li-xing, Yang, Yao-jun, Wu, Hao-zhong, Wei, Feng, Zhang, Ji, Tian, Jin-gai, and Lin, Rui-chao
- Subjects
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CHINESE medicine , *ELECTRONIC noses , *GINSENG , *CHEMOMETRICS , *NONDESTRUCTIVE testing , *PRINCIPAL components analysis , *DISCRIMINANT analysis - Abstract
Abstract: Red ginseng is a precious and widely used traditional Chinese medicine. At present, Chinese red ginseng and Korean ginseng are both commonly found on the market. To rapidly and nondestructively discriminate between Chinese red ginseng and Korean ginseng, an electronic nose coupled with chemometrics was developed. Different red ginseng samples, including Chinese red ginseng (n =30) and Korean ginseng (South Korean red ginseng and North Korean red ginseng n =26), were collected. The metal oxide sensors on an electronic nose were used to measure the red ginseng samples. Multivariate statistical analyses, including principal component analysis (PCA), discriminant factorial analysis (DFA) and soft independent modeling of class analogy (SIMCA), were employed. All of the samples were analyzed by PCA. Most of the samples were used to set up DFA and SIMCA models, and then the remaining samples (Nos. 9, 10, 17, 18, 29, 30, 34, 43, 44, 50, and 51) were projected onto the DFA and SIMCA models in the form of black dots to validate the models. The results indicated that Chinese red ginseng and Korean ginseng were successfully discriminated using the electronic nose coupled with PCA, DFA and SIMCA. The checking scores of the DFA and SIMCA models were 100. The samples projected onto the DFA and SIMCA models were all correctly discriminated. The DFA and SIMCA models were robust. Electronic nose technology is a rapid, accurate, sensitive and nondestructive method to discriminate between Chinese red ginseng and Korean ginseng. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
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