1. Polyclonal, newly derived T cells with low expression of inhibitory molecule PD-1 in tonsils define the phenotype of lymphocytes in children with Periodic Fever, Aphtous Stomatitis, Pharyngitis and Adenitis (PFAPA) syndrome
- Author
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Petra, Dytrych, Petra, Krol, Dytrych, Petra, Krol, Petra, Michaela, Kotrova, Daniela, Kuzilkova, Kotrova, Michaela, Kuzilkova, Daniela, Petr, Hubacek, Ladislav, Krol, Hubacek, Petr, Krol, Ladislav, Rami, Katra, Ondrej, Hrusak, Katra, Rami, Hrusak, Ondrej, Zdenek, Kabelka, Pavla, Dolezalova, Kabelka, Zdenek, Dolezalova, Pavla, Tomas, Kalina, Eva, Fronkova, Kalina, Tomas, and Fronkova, Eva
- Subjects
Male ,Herpesvirus 4, Human ,PFAPA syndrome ,Pathology ,medicine.medical_specialty ,Herpesvirus 6, Human ,Lymphocyte ,Palatine Tonsil ,Programmed Cell Death 1 Receptor ,Immunology ,Receptors, Antigen, T-Cell ,CD8-Positive T-Lymphocytes ,Chemokine CXCL9 ,Fever of Unknown Origin ,Adenoviridae ,Pathogenesis ,Lymphadenitis ,T-Lymphocyte Subsets ,medicine ,Humans ,Cytotoxic T cell ,Lymphocyte Count ,Child ,Molecular Biology ,Tonsillectomy ,B-Lymphocytes ,Sleep Apnea, Obstructive ,biology ,business.industry ,T-cell receptor ,Infant ,Pharyngitis ,T lymphocyte ,Chemokine CXCL10 ,medicine.anatomical_structure ,Polyclonal B cell response ,Child, Preschool ,biology.protein ,Chemokine CCL19 ,Female ,Stomatitis, Aphthous ,Antibody ,business - Abstract
Purpose PFAPA syndrome is a benign, recurrent inflammatory disease of childhood. Tonsillectomy is one of the therapeutic options with a yet unexplained biological mechanism. We tested whether specific lymphocyte subsets recruited from blood to human tonsils participate in PFAPA pathogenesis. Methods Paired tonsils/peripheral blood (PB) samples were investigated (a) from children with PFAPA that successfully resolved after tonsillectomy ( n = 10) (b) from children with obstructive sleep apnoea syndrome as controls ( n = 10). The lymphocyte profiles were analysed using 8-colour flow cytometry, immunoglobulin (IGH) and T-cell receptor (TCR) gene rearrangements via PCR and next generation sequencing; a TREC/KREC analysis was performed using qPCR. Results The PFAPA tonsils in the asymptomatic phase had a lower percentage of B-lymphocytes than controls; T-lymphocyte counts were significantly higher in PB. The percentages of cytotoxic CD8pos T-lymphocytes were approximately 2-fold higher in PFAPA tonsils; the transitional B cells and naive stages of both the CD4pos and CD8pos T-lymphocytes with a low expression of PD-1 molecule and high numbers of TREC were also increased. With the exception of elevated plasmablasts, no other differences were significant in PB. The expression levels of CXCL10, CXCL9 and CCL19 genes were significantly higher in PFAPA tonsils. The IGH/TCR pattern showed no clonal/oligoclonal expansion. DNA from the Epstein-Barr virus, Human Herpervirus-6 or adenovirus was detected in 7 of 10 PFAPA tonsils but also in 7 of 9 controls. Conclusions Our findings suggest that the uninhibited, polyclonal response of newly derived lymphocytes participate in the pathogenesis of PFAPA. Because most of the observed changes were restricted to tonsils and were not present in PB, they partly explain the therapeutic success of tonsillectomy in PFAPA syndrome.
- Published
- 2015
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