11 results on '"Dana Huang"'
Search Results
2. Soaring Asymptomatic Infected Individuals Bring About Barriers and Difficulties for Interruption of COVID-19 Prevalence in China
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Tiejian Feng, Dana Huang, Shujiang Mei, Shun-Xian Zhang, Mu-Xin Chen, Ai Lin, Yalan Huang, Jia-Xu Chen, Xiao-Nong Zhou, Bo Peng, and Renli Zhang
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Pediatrics ,medicine.medical_specialty ,China ,Time Factors ,Microbiology ,Asymptomatic ,Cohort Studies ,Virology ,Pandemic ,Prevalence ,Medicine ,Humans ,Asymptomatic Infections ,Retrospective Studies ,business.industry ,SARS-CoV-2 ,Medical record ,COVID-19 ,Odds ratio ,Confidence interval ,Infectious Diseases ,Cohort ,Observational study ,medicine.symptom ,business - Abstract
Background: Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic, which has caused unprecedented damage to human health and life. The present study aimed to carry out and discover asymptomatic infected individuals in Shenzhen, China. The data will provide the control measures to stop COVID-19 prevalence. Methods: The study was a retrospective review of medical records from 462 confirmed patients with COVID-19 and 45 asymptomatic infected individuals in Shenzhen from January 19 to April 30, 2020; this is a retrospective, observational multicenter study. Results: A total of 462 confirmed cases were diagnosed in Shenzhen from January 19 to April 30, 2020. The cohort included 423 domestic cases (91.56%, 95% confidence interval [CI]: 88.67-93.76) and 39 (8.44%, 95% CI: 6.24-11.33) imported cases from other countries. Moreover, a total of 45 asymptomatic infections were found, encompassing 31 (68.89%, 95% CI: 54.34-80.47) local infections and 14 (31.11%, 95% CI: 19.53-45.66) individuals imported from other countries. The proportion of asymptomatic infected persons in Shenzhen is continuously increasing (Z = 13.19, p < 0.0001). The total number of local asymptomatic infections was more than that in other provinces (χ2 = 118.83, p < 0.0001). The proportion of asymptomatic infected individuals among cases imported from other countries was higher than the domestic cases (χ2 = 22.51, p < 0.0001, odds ratio = 4.90, 95% CI: 2.40-9.98). Conclusions: The proportion of asymptomatic infection is increasing. Hence, development and application of the diagnosis method with high sensitivity and specificity play a critical role in reducing COVID-19 global epidemics.
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- 2021
3. The First Reported Case of COVID-19 and Plasmodium ovale Malaria Coinfection — Guangdong Province, China, January 2021
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Lin Ai, Yijun Tang, Deng Shaoyu, Qian Zhang, Huawei Xiong, Xiao-Nong Zhou, Tiejian Feng, Lin Zidan, Mu-Xin Chen, Zhigao Chen, Dana Huang, Jia-Xu Chen, Gengcheng He, Shitong Gao, Renli Zhang, and Xiaomin Zhang
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Coronavirus disease 2019 (COVID-19) ,Environmental health ,MEDLINE ,Coinfection ,medicine ,Biology ,General Agricultural and Biological Sciences ,medicine.disease ,China ,Plasmodium ovale malaria - Published
- 2021
4. Differences in the Transmission of Dengue Fever by Different Serotypes of Dengue Virus
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Suibin Huang, Yalan Huang, Cong Niu, Jia Li, Chengsong Wan, Miao Wang, Dana Huang, Fan Yang, and Renli Zhang
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0301 basic medicine ,Serotype ,China ,Aedes albopictus ,viruses ,030231 tropical medicine ,Aedes aegypti ,Mosquito Vectors ,Biology ,Dengue virus ,medicine.disease_cause ,Serogroup ,Virus Replication ,Microbiology ,Arbovirus ,Dengue fever ,Dengue ,03 medical and health sciences ,0302 clinical medicine ,Aedes ,Virology ,Chlorocebus aethiops ,medicine ,Animals ,Humans ,Vero Cells ,Transmission (medicine) ,virus diseases ,biochemical phenomena, metabolism, and nutrition ,030108 mycology & parasitology ,Dengue Virus ,medicine.disease ,biology.organism_classification ,Infectious Diseases ,Viral replication - Abstract
Dengue fever is the most common arbovirus in the world, and all four serotypes are infectious and pathogenic, but dengue virus (DENV)-1 and DENV-2 are the most prevalent in Guangdong Province. To investigate the differences in serotypes, we evaluated the replication, pathogenicity, and NS1 expression of DENV-2 and DENV-3 in cells, and further investigated the effects of NS1 on viral replication and transmission. The results of this study indicated that DENV-2 replicated faster and was more pathogenic than DENV-3. In Vero cells, DENV-2 secreted more NS1 protein than DENV-3, and NS1 protein promoted the infection of Aedes albopictus and A. aegypti by DENV. The spread of DENV and the severity of the disease depend on many factors. Further research on the differences between different serotypes of DENV will help us understand the epidemiology of DENV infection. This will provide a scientific basis for the prevention and control of the dengue epidemic.
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- 2019
5. Survey of
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Dana, Huang, Yalan, Huang, Yijun, Tang, Qian, Zhang, Xiaoheng, Li, Shitong, Gao, Wuwei, Hua, and Renli, Zhang
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Adult ,Male ,China ,Adolescent ,Snails ,Antibodies, Helminth ,Angiostrongylus cantonensis ,Infant ,Enzyme-Linked Immunosorbent Assay ,Rodentia ,Middle Aged ,Rodent Diseases ,Seroepidemiologic Studies ,Child, Preschool ,Immunoglobulin G ,Outpatients ,Prevalence ,Animals ,Humans ,Female ,Child ,Strongylida Infections - Abstract
The study was to understand
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- 2019
6. Identification and characterization of the causative triatomine bugs of anaphylactic shock in Zhanjiang, China
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Li-Fei Peng, Yalan Huang, Renli Zhang, Weihua Wu, Xiaomin Zhang, Miao Wang, Dana Huang, Yijun Tang, Fan Yang, and Qian Zhang
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Male ,0301 basic medicine ,Chagas disease ,China ,Mitochondrial DNA ,Trypanosoma cruzi ,030231 tropical medicine ,Zoology ,Biology ,Anaphylactic shock ,lcsh:Infectious and parasitic diseases ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Blood serum ,Species Specificity ,law ,RNA, Ribosomal, 16S ,RNA, Ribosomal, 28S ,parasitic diseases ,RNA, Ribosomal, 18S ,medicine ,Animals ,Humans ,lcsh:RC109-216 ,Triatoma ,Anaphylaxis ,Phylogeny ,Polymerase chain reaction ,lcsh:Public aspects of medicine ,fungi ,Cytochrome c oxidase subunit I ,Public Health, Environmental and Occupational Health ,lcsh:RA1-1270 ,DNA ,General Medicine ,Allergens ,Ribosomal RNA ,medicine.disease ,biology.organism_classification ,Triatoma rubrofasciata ,030104 developmental biology ,Infectious Diseases ,Female ,Trypanosomiasis ,Research Article - Abstract
Background Two health concerns primarily related to triatomine bugs are transmission of Trypanosoma cruzi through infective feces, and allergic reactions induced by triatomine bites. In the Southwestern United States, reduviid bugs bites commonly cause insect allergy. In South China, four cases of anaphylactic shock have been reported after this bite exposure. To further classify the species of these bugs and confirm the sensitization of the triatomine saliva, we caught triatomine bugs from the region where the bites occurred and performed phylogenetic and immunohistochemical (IHC) analysis. Methods Triatomine bugs were collected in Donghai Island of Zhanjiang City in South China. The genomic DNA was extracted from three legs of the bugs. The fragments of mitochondrial 16S rRNA, cytochrome c oxidase subunit I (COI) gene and nuclear ribosomal 18S and 28S rRNA genes were obtained by PCR and sequenced. A phylogenetic tree was constructed based on the sequence of 16S rRNA gene using a maximum likelihood method with MEGA 7.0 software. Trypanosomal specific fragments and vertebrate COI genes were amplified from the fecal DNA to detect the infection of trypanosomes and analyze the blood feeding patterns, respectively. Paraffin-embedded sections were then prepared from adult triatomines and sent for IHC staining. Results We collected two adult triatomine bugs in Donghai Island. Morphological and molecular analyses indicated that the triatomines were Triatoma rubrofasciata. No fragments of T. cruzi or other trypanosomes were detected from the fecal DNA. Mitochondrial gene segments of Homo sapiens and Mus musculus were successfully amplified. The allergens which induced specific IgE antibodies in human serum were localized in the triatomine saliva by IHC assay. Conclusions The two triatomine bugs from Donghai Island were T. rubrofasciata. They had bitten humans and mice. Their saliva should contain the allergens related to the allergic symptoms and even anaphylactic shock of exposed residents. Great consideration should be given to this triatomine bugs due to their considerable distribution and potential threat to public health in South China.
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- 2018
7. Epidemiological and Genetic Characteristics of Rabies Virus Transmitted Through Organ Transplantation
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Jingfang Chen, Guang Liu, Tao Jin, Rusheng Zhang, Xinhua Ou, Heng Zhang, Peng Lin, Dong Yao, Shuilian Chen, Meiling Luo, Fan Yang, Dana Huang, Biancheng Sun, and Renli Zhang
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0301 basic medicine ,Male ,lcsh:QR1-502 ,rabies ,medicine.disease_cause ,Antibodies, Viral ,Kidney ,Organ transplantation ,lcsh:Microbiology ,0302 clinical medicine ,Postoperative Complications ,030212 general & internal medicine ,Phylogeny ,Original Research ,Sanger sequencing ,Transmission (medicine) ,organ transplantation ,transmission ,Middle Aged ,Tissue Donors ,Infectious Diseases ,Liver ,symbols ,Female ,Encephalitis ,Microbiology (medical) ,Adult ,medicine.medical_specialty ,China ,Immunology ,Microbiology ,03 medical and health sciences ,symbols.namesake ,medicine ,Humans ,business.industry ,phylogenetic analysis ,Rabies virus ,Infant ,medicine.disease ,Dog bite ,Virology ,Kidney Transplantation ,Liver Transplantation ,030104 developmental biology ,Etiology ,Rabies ,next-generation sequencing ,business - Abstract
In January 2016, two patients died of rabies after receiving kidney transplants from a common organ donor at a hospital in Changsha, Hunan, China. The medical records, epidemiological data of the organ donor, two kidney and a liver recipients were reviewed. Intravitam saliva samples of the two kidney recipients were tested for rabies virus (RABV) using real-time RT-PCR, and the nucleoprotein (N) gene was amplified and sequenced by Sanger sequencing. Whole genome sequences were analyzed using next-generation sequencing. The N genes of the two kidney recipients showed 100% nucleic acid identity. Phylogenetic analysis of the complete genome, N and glycoprotein (G) genes indicated that the RABV was homologous with dog isolates from the Hunan province and belong to the China I lineage, which is widespread in China. The organ donor was a 22-month-old boy who died from unknown acute progressive encephalitis. After undergoing sub-hypothermia hibernation therapy, rabies-associated symptoms were atypical, and rabies was neglected because serum RABV-specific antibodies were negative. An unknown wound on the forehead of the donor was found 2 months before the onset of symptoms. Based on the clinical, epidemiological, and molecular findings, we speculated that the RABV initially originated in the donor from a dog bite, and was then transmitted to the recipients by organ transplantation. An uncertain exposure history and misdiagnosis played important roles in the spread of the RABV. Rabies should be considered in patients with acute progressive encephalitis of unexplained etiology, especially in potential organ donors.
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- 2018
8. Genome Sequence of a Novel Strain of a Phasi Charoen-Like Virus Identified in Zhanjiang, China
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Xiaomin Zhang, Chengsong Wan, Tao Jin, Yang Liu, Yalan Huang, Chunli Wu, Dana Huang, Jinquan Cheng, Peng Lin, Renli Zhang, Suibin Huang, Jianbin Xie, and Fan Yang
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0301 basic medicine ,Whole genome sequencing ,Genetics ,biology ,Phylogenetic tree ,Strain (biology) ,viruses ,Aedes aegypti ,biology.organism_classification ,Virus ,03 medical and health sciences ,Phasi Charoen-like virus ,030104 developmental biology ,Genus ,Viruses ,China ,Molecular Biology - Abstract
Here, we report the genome sequence of a novel strain of a Phasi Charoen-like virus, isolated from Aedes aegypti mosquitoes caught in Zhanjiang province of China. Phylogenetic analysis suggested that this virus belongs to a new genus, Goukovirus , in the family Bunyaviridae . This is the first reported genome sequence of a Phasi Charoen-like virus identified in China.
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- 2018
9. An Integrated Control Strategy Takes Clonorchis sinensis Under Control in an Endemic Area in South China
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Hailong Zhang, Yijie Geng, Jianping Cao, Ran Zhang, Yalan Huang, Fan Yang, Shisong Fang, Dana Huang, Shuang Wu, Chunli Wu, Xin Wang, Miao Wang, and Renli Zhang
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0301 basic medicine ,Veterinary medicine ,China ,Time Factors ,Endemic Diseases ,030231 tropical medicine ,Snails ,Biology ,Microbiology ,Praziquantel ,03 medical and health sciences ,Feces ,0302 clinical medicine ,Food Parasitology ,Virology ,medicine ,Prevalence ,Animals ,Humans ,Longitudinal Studies ,Toilet Facilities ,Eggs per gram ,Schistosoma ,Anthelmintics ,Clonorchis sinensis ,Zoonosis ,Fishes ,030108 mycology & parasitology ,biology.organism_classification ,medicine.disease ,Infectious Diseases ,Clonorchiasis ,medicine.drug - Abstract
Clonorchis sinensis is an important foodborne zoonosis worldwide and prevalent in China for more than 2000 years. According to the experience of controlling Schistosoma japonica, China started to establish the integrated control strategy for C. sinensis in endemic areas. Lou village, the largest village in Shenzhen city in South China was taken as a pilot site. This longitudinal study assessed the infection status of C. sinensis among people and intermediate hosts from 2006 to 2014 in Lou village. After a continuous intervention with the integrated control strategy, the prevalence of C. sinensis decreased significantly to 2.01% in 2014. The infection intensity also reduced significantly with eggs per gram varying from 45.6 ± 3.4 in 2010 to 21.7 ± 1.6 in 2012. There is also a statistically significant decrease of the prevalence of C. sinensis metacercariae in fish hosts from 16.51% in 2008 before the intervention to 5.33% in 2014. All the old-styled toilets were replaced by sanitary ones with a harmless processing design in 2014. No viable parasite eggs were detected in stool samples from the reconstructed toilets. Health education played an important role in changing the eating habits among the local residents, with a significant decrease in the prevalence of eating raw fish from 91.99% in 2008 to 59.87% in 2014. The evaluation suggested that the integrated strategy we have performed in Lou village is effective in controlling the C. sinensis infection and maintaining the infection rate at a lower level, which can be promoted in other endemic areas.
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- 2017
10. Epidemiological study on Clonorchis sinensis infection in Shenzhen area of Zhujiang delta in China
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Yu-Cai Fu, Yijie Geng, Jingquan Cheng, Lei Yu, Renli Zhang, Shun-Xiang Zhang, Shitong Gao, and Dana Huang
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Adult ,Male ,China ,Veterinary medicine ,Adolescent ,Snails ,Population ,Seroepidemiologic Studies ,medicine ,Animals ,Humans ,Child ,education ,Mass screening ,Feces ,Eggs per gram ,education.field_of_study ,Clonorchis sinensis ,General Veterinary ,biology ,Fishes ,Intermediate host ,General Medicine ,Middle Aged ,biology.organism_classification ,medicine.disease ,Infectious Diseases ,Insect Science ,Clonorchiasis ,Freshwater fish ,Female ,Parasitology - Abstract
To study the transmission route and epidemiological features of Clonorchis sinensis infection in Shenzhen area, which is the biggest immigration city in the south of China, we examined 1,473 individuals (710 males and 763 females) to assess the current status of C. sinensis infection among the people in a village of Shenzhen in Zhujiang delta of Guangdong province, China. Freshwater snails, 630, of different species known as the first intermediate host of C. sinensis were collected and examined for cercaria infection, and 430 freshwater fishes of different species as the second intermediate host were examined for metacercaria infection. Among 1,473 people examined, 70 (4.75%) were found infected with C. sinensis. By counting eggs per gram feces (EPG), it was found that the intensity of infection in males was stronger than that of females, and the average EPG was 41.87 in all population. Snails, 1.15%, were infected with cercariae of C. sinensis. The average infection rate of freshwater fishes of 15 species with metacercariae of C. sinensis was 16.97%, and the carps reached the highest infection rate (40.74%). A questionnaire was designed with 12 questions covering socioeconomic conditions and human behavior, contamination of the environment, and fishponds. Of 1,473 interviewees, 54% did not know about fluke disease or its transmission route, 12% of those who knew about the fluke believed that the infection causes no harm or only slight harm to their health. Of the interviewees, 27%, ate raw fish at least one to two times per month. Of families, 5% used the same utensils for both raw fish and cooked food. Of the fishpond owners, 40% fed their fishes with feces of domestic animals and humans. All these factors of unhealthy behaviors, poor knowledge, inappropriate farming/fishery practices, and eating raw fish have made the prevalence of clonorchiasis increase in humans in the Shenzhen area. It is urgent to perform a control program, including health education, environmental modification, reform of traditional farming/fishery practice, mass screening, and chemotherapy for humans, and the management of domestic animals to decrease C. sinensis infection in the human population in Shenzhen.
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- 2007
11. Seroprevalence of Angiostrongylus cantonensis Infection in Humans in China
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Renli Zhang, Jia-Xu Chen, Xing-Quan Zhu, Dana Huang, Mu-Xin Chen, Shao-Hong Chen, Xiaoheng Li, Lin Ai, Shitong Gao, and Yijie Geng
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Male ,China ,Veterinary medicine ,Occupational group ,Angiostrongylus cantonensis infection ,Seroepidemiologic Studies ,medicine ,Animals ,Humans ,Seroprevalence ,Ecology, Evolution, Behavior and Systematics ,Strongylida Infections ,Chromatography ,biology ,Angiostrongylus cantonensis ,biology.organism_classification ,medicine.disease ,Immunohistochemistry ,Pomacea ,Achatina ,Antigens, Helminth ,Angiostrongyliasis ,Female ,Parasitology - Abstract
A seroepidemiological survey was carried out in China during 2009-2010 to determine the extent of circulating antigens (CAg) for Angiostrongylus cantonensis in the Chinese population using the gold immunochromatographic assay, with the objective of elucidating the nationwide prevalence of angiostrongyliasis in China. A total of 1,730 blood samples was collected and assayed from the general adult population (the "general group"), and those involved in aquaculture or processing of snails Achatina fulica and Pomacea canaliculat (the "occupational group") from 5 provinces (Fujian, Hunan, Guangdong, Guangxi, and Zhejiang) and 1 municipal city (Beijing). The overall seroprevalence for the "occupational group" was 7.4% (40/540), which was significantly higher (P0.001) than that of the "general group" (0.8%, 9/1,190). The seroprevalence in males (9.5%) was significantly higher than in females (4.2%) (P0.05). These results demonstrate that angiostrongyliasis represents a significant zoonotic disease in China, requiring the strengthening of food safety for control of this food-borne disease.
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- 2011
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