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Hemorrhage is a leading cause of preventable death in both military combat and civilian accidents. To overcome these challenges, an affordable and effective bandage is must required substance. A novel strategy is reported for developing chitosan-casein (CC) based self-assembled nanofibrous polyelectrolyte complex (PEC) for rapid blood clotting. The amide group (1630 cm -1 ) and phosphate group (910 cm -1 ) of chitosan-casein can form PEC at pH 8.2 ± 0.2. The PECs contain intertwined nanofibers (≤100 nm diameter) with a high surface area. Increasing chitosan percentage from 30% (CC30) to 50% (CC50) or 70% (CC70) results, increase in zeta potential of PEC from -9.14 ± 3.3 to 7.46 ± 3.7 and 14.8 ± 3.3 mV, respectively. Under in vitro conditions, the CC30, CC50, and CC70 PECs allow platelet adhesion and rapidly absorbs blood fluid to form mechanically stable blood clots within 9 ± 3, 16 ± 3, and 30 ± 4 s, respectively, which are better than Celox™ (90 ± 3 s). In vivo application of PEC (CC50) causes clotting within 37 ± 6 s of large (1 cm) arterial incision in rabbit models. The PEC is biocompatible with promising hemostatic efficiency. This is the first report of nanofibrous PEC from chitosan and casein for rapid clotting, to the best of our knowledge., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The complexation processes of chitosan with cobalt(II)tetrasulfophthalocyanine (CoPc) and copper(II)tetrasulfophthalocyanine (CuPc) were studied calorimetrically in solution. It was established that CoPc forms two types of complexes with chitosan, while CuPc forms a single type of complex with chitosan, in which copper(II)tetrasulfophthalocyanine is in dimerized form. The complexes are thermodynamically stable, which was allowed to study them in a solid form by different methods. Joint application of DSC and TG/DTG methods allowed us to identify the temperature intervals for evaporation of physically and chemically bounded water and thermal decomposition of chitosan and its complexes. The glass transition temperature of chitosan (110.8 °C) is greater than the glass transition temperature of the complexes with CuPc (74.7 °C) and CoPc (71.2 °C). Using SEM images and X-ray data of heated, unheated chitosan and its complexes, it was shown that the complexes are predominantly amorphous. Heating of chitosan and its leads to increasing of amorphous phase. Modification of chitosan by phthalocyanines leads to decreasing of thermal stability of complexes insignificantly., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Chitosan is a naturally occurring polysaccharide derived from chitin with a wide range of uses. Phthalocyanines are macroheterocyclic compounds that have a number of useful properties such as coloring and catalytic and antioxidant activity. Phthalocyanines are able to immobilize on chitosan, forming complexes with new useful properties. In this work, we evaluated the ability of phthalocyanines to increase the thermal stability of chitosan. Chitosan (CS) forms complexes with copper(II)-(CuPc) and cobalt(II)-(CoPc) tetrasulphophthalocyanines. The processes of destruction of chitosan (CS) and its complexes with sulphophthalocyanines CuPc and CoPc in oxidizing and inert atmospheres have been studied. It was established that, regardless of the atmosphere composition, the first chemical reactions taking place in the studied systems are elimination reactions. The latter ones in the case of chitosan and complex CS-CuPc lead to the formation of spatially crosslinked polymer structures, and it causes the release of CuPc from the polymer complex. It was found that in the case of CS-CoPc elimination reactions did not lead to the formation of crosslinked polymer structures but caused the destruction of the pyranose rings with a partial release of CoPc. Metallophthalocyanines showed antioxidant properties in the composition of complexes with chitosan, increasing the temperature of the beginning of glycosidic bond cleavage reaction by 30–35 °C in comparison with the similar characteristics for chitosan. [ABSTRACT FROM AUTHOR]