1. Chlamydia psittaci detected at a live poultry wholesale market in central China.
- Author
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Zhang, Rusheng, Fu, Huiyuan, Luo, Can, Huang, Zheng, Pei, Ruiqing, Di, Yu, Zhu, Caiying, Peng, Jiayi, Hu, Huiqi, Chen, Shan, Chen, Jingfang, Chen, Lamei, Xu, Mingzhong, Yang, Xuewen, and Yang, Rengui
- Subjects
PIGEONS ,POULTRY ,CHLAMYDIA ,CHICKENS ,POLYMERASE chain reaction ,ENVIRONMENTAL sampling - Abstract
Background: We investigated the presence of Chlamydia psittaci in poultry and the environment in live poultry wholesale markets in Changsha during 2021–2022 and conducted a phylogenetic analysis to understand its distribution in this market. Methods: In total, 483 samples were analyzed using real-time polymerase chain reaction and 17 C. psittaci-positive samples using high-throughput sequencing, BLAST similarity, and phylogenetic analysis. Results: Twenty-two out of 483 poultry and environmental samples were positive for C. psittaci (overall positivity rate: 4.55%) with no difference in positivity rates over 12 months. Chlamydia psittaci was detected at 11 sampling points (overall positivity rate: 27.5%), including chicken, duck, and pigeon/chicken/duck/goose shops, with pigeon shops having the highest positivity rate (46.67%). The highest positivity rates were found in sewage (12.5%), poultry fecal (7.43%), cage swab (6.59%), avian pharyngeal/cloacal swab (3.33%), and air (2.29%) samples. The ompA sequences were identified in two strains of C. psittaci, which were determined to bear genotype B using phylogenetic analysis. Thus, during monitoring, C. psittaci genotype B was detected in the poultry and environmental samples from the poultry wholesale market in Changsha. Conclusions: To address the potential zoonotic threat, C. psittaci monitoring programs in live poultry markets should be enhanced. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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