1. Productive and Penicillin-Stressed Chlamydia pecorum Infection Induces Nuclear Factor Kappa B Activation and Interleukin-6 Secretion In Vitro .
- Author
-
Leonard CA, Schoborg RV, and Borel N
- Subjects
- Animals, Caco-2 Cells, Chlamydia drug effects, Chlamydia trachomatis drug effects, Chlamydia trachomatis pathogenicity, Chlorocebus aethiops, HeLa Cells, Humans, Inflammation immunology, Penicillins pharmacology, Species Specificity, Stress, Physiological drug effects, Swine, Vero Cells, Chlamydia pathogenicity, Chlamydia Infections immunology, Host-Pathogen Interactions immunology, Interleukin-6 metabolism, NF-kappa B metabolism
- Abstract
Nuclear factor kappa B (NFκB) is an inflammatory transcription factor that plays an important role in the host immune response to infection. The potential for chlamydiae to activate NFκB has been an area of interest, however most work has focused on chlamydiae impacting human health. Given that inflammation characteristic of chlamydial infection may be associated with severe disease outcomes or contribute to poor overall fitness in farmed animals, we evaluated the ability of porcine chlamydiae to induce NFκB activation in vitro . C. pecorum infection induced both NFκB nuclear translocation and activation at 2 hours post infection (hpi), an effect strongly enhanced by suppression of host de novo protein synthesis. C. suis and C. trachomatis showed less capacity for NFκB activation compared to C. pecorum , suggesting a species-specific variation in NFκB activation. At 24 hpi, C. pecorum induced significant NFκB activation, an effect not abolished by penicillin (beta lactam)-induced chlamydial stress. C. pecorum -dependent secretion of interleukin 6 was also detected in the culture supernatant of infected cells at 24 hpi, and this effect, too, was unchanged by penicillin-induced chlamydial stress. Taken together, these results suggest that NFκB participates in the early inflammatory response to C. pecorum and that stressed chlamydiae can promote inflammation.
- Published
- 2017
- Full Text
- View/download PDF