1. Chemical Genetics Approach Reveals Importance of cAMP and MAP Kinase Signaling to Lipid and Carotenoid Biosynthesis in Microalgae.
- Author
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Choi YE, Rhee JK, Kim HS, Ahn JW, Hwang H, and Yang JW
- Subjects
- Carotenoids analysis, Glucose metabolism, Lipids analysis, Reactive Oxygen Species metabolism, Signal Transduction physiology, Carotenoids biosynthesis, Chlamydomonas reinhardtii chemistry, Chlamydomonas reinhardtii metabolism, Cyclic AMP metabolism, Lipids biosynthesis, Mitogen-Activated Protein Kinases metabolism
- Abstract
In this study, we attempted to understand signaling pathways behind lipid biosynthesis by employing a chemical genetics approach based on small molecule inhibitors. Specific signaling inhibitors of MAP kinase or modulators of cAMP signaling were selected to evaluate the functional roles of each of the key signaling pathways in three different microalgal species: Chlamydomonas reinhardtii, Chlorella vulgaris, and Haematococcus pluvialis. Our results clearly indicate that cAMP signaling pathways are indeed positively associated with microalgal lipid biosynthesis. In contrast, MAP kinase pathways in three microalgal species are all negatively implicated in both lipid and carotenoid biosynthesis.
- Published
- 2015
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