1. Anti-serotonergic effects of urethane and chloral hydrate may not be mediated by a blockade of 5-HT2 receptors. Short communication.
- Author
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Dringenberg HC, Baker GB, Urichuk LJ, and Vanderwolf CH
- Subjects
- Animals, Binding, Competitive physiology, Ethylene Chlorohydrin analogs & derivatives, Ethylene Chlorohydrin pharmacology, Ketanserin metabolism, Ketanserin pharmacology, Male, Membrane Proteins antagonists & inhibitors, Rats, Rats, Inbred Strains, Receptors, Serotonin metabolism, Synaptic Transmission drug effects, Tritium, Anesthetics, Intravenous pharmacology, Chloral Hydrate pharmacology, Serotonin Antagonists pharmacology, Urethane pharmacology
- Abstract
The general anesthetics urethane and chloral hydrate have profound anti-serotonergic effects both in the rat cortex in vivo and the rat aortic ring in vitro. The suggestion that these effects may be due to an action on 5-HT2 receptors was tested using ex vivo and in vitro [3H]ketanserin binding assays with membrane-enriched fractions from rat brain. Urethane did not alter [3H]ketanserin binding in the ex vivo assay. In the in vitro assay, urethane, chloral hydrate, and its active metabolite 2,2,2-trichloroethanol produced slight reductions (of 16%, 9%, and 18%, respectively) of [3H]ketanserin binding. These studies suggest that anti-serotonergic effects of urethane and chloral hydrate may not be mediated by a blockade of 5-HT2 receptors.
- Published
- 1996
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