1. white panicle2 encoding thioredoxin z, regulates plastid RNA editing by interacting with multiple organellar RNA editing factors in rice.
- Author
-
Wang, Yunlong, Wang, Yihua, Ren, Yulong, Duan, Erchao, Zhu, Xiaopin, Hao, Yuanyuan, Zhu, Jianping, Chen, Rongbo, Lei, Jie, Teng, Xuan, Zhang, Yuanyan, Wang, Di, Zhang, Xin, Guo, Xiuping, Jiang, Ling, Liu, Shijia, Tian, Yunlu, Liu, Xi, Chen, Liangming, and Wang, Haiyang
- Subjects
- *
RNA editing , *THIOREDOXIN , *RICE , *GENES , *PLANT RNA , *FORKHEAD transcription factors , *CHLOROPLASTS - Abstract
Summary: Thioredoxins (TRXs) occur in plant chloroplasts as complex disulphide oxidoreductases. Although many biological processes are regulated by thioredoxins, the regulatory mechanism of chloroplast TRXs are largely unknown.Here we report a rice white panicle2 mutant caused by a mutation in the thioredoxin z gene, an orthologue of AtTRX z in Arabidopsis. white panicle2 (wp2) seedlings exhibited a high‐temperature‐sensitive albinic phenotype.We found that plastid multiple organellar RNA editing factors (MORFs) were the regulatory targets of thioredoxin z. We showed that OsTRX z protein physically interacts with OsMORFs in a redox‐dependent manner and that the redox state of a conserved cysteine in the MORF box is essential for MORF–MORF interactions. wp2 and OsTRX z knockout lines show reduced editing efficiencies in many plastidial‐encoded genes especially under high‐temperature conditions. An Arabidopsis trx z mutant also exhibited significantly reduced chloroplast RNA editing.Our combined results suggest that thioredoxin z regulates chloroplast RNA editing in plants by controlling the redox state of MORFs. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF