1. A PLCB1-PI3K-AKT Signaling Axis Activates EMT to Promote Cholangiocarcinoma Progression.
- Author
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Liang S, Guo H, Ma K, Li X, Wu D, Wang Y, Wang W, Zhang S, Cui Y, Liu Y, Sun L, Zhang B, Xin M, Zhang N, Zhou H, Liu Y, Wang J, and Liu L
- Subjects
- Animals, Antineoplastic Combined Chemotherapy Protocols pharmacology, Apoptosis, Bile Duct Neoplasms drug therapy, Bile Duct Neoplasms genetics, Bile Duct Neoplasms metabolism, Biomarkers, Tumor genetics, Biomarkers, Tumor metabolism, Carcinogenesis, Cell Movement, Cell Proliferation, Cholangiocarcinoma drug therapy, Cholangiocarcinoma genetics, Cholangiocarcinoma metabolism, Cisplatin administration & dosage, Deoxycytidine administration & dosage, Deoxycytidine analogs & derivatives, Gene Expression Regulation, Neoplastic, Humans, Male, Mice, Mice, Inbred BALB C, Mice, Nude, Neoplasm Invasiveness, Phosphatidylinositol 3-Kinases genetics, Phospholipase C beta genetics, Prognosis, Proto-Oncogene Proteins c-akt genetics, Tumor Cells, Cultured, Xenograft Model Antitumor Assays, Gemcitabine, Bile Duct Neoplasms pathology, Cholangiocarcinoma pathology, Drug Resistance, Neoplasm, Epithelial-Mesenchymal Transition, Phosphatidylinositol 3-Kinases metabolism, Phospholipase C beta metabolism, Proto-Oncogene Proteins c-akt metabolism
- Abstract
As a member of the phospholipase family, phospholipase C beta 1 ( PLCB1 ) is involved in phospholipid hydrolysis and is frequently upregulated in human cancer. However, little is known about the role of PLCB1 in cholangiocarcinoma (CCA). In this study, we uncover a role for PLCB1 in CCA progression and identify the underlying mechanisms. Both human CCA tissues and CCA cell lines expressed high levels of PLCB1. PLCB1 promoted tumor development and growth in various CCA mouse models, including transposon-based tumorigenesis models. PLCB1 activated PI3K/AKT signaling to induce CCA cells to undergo epithelial-to-mesenchymal transition (EMT). Mechanistically, PABPC1 interacted with PLCB1 and PI3K to amplify PLCB1-mediated EMT via PI3K/AKT/GSK3β/Snail signaling. Ectopic PLCB1 induced resistance to treatment with gemcitabine combined with cisplatin, which could be reversed by the AKT inhibitor MK2206. PLCB1 expression was regulated by miR-26b-5p through direct interaction with PLCB1 3'UTR. Collectively, these data identify a PLCB1-PI3K-AKT signaling axis vital for CCA development and EMT, suggesting that AKT can be used as a therapeutic target to overcome chemotherapy resistance in CCA patients with high PLCB1 expression. SIGNIFICANCE: PLCB1 functions as an oncogenic driver in cholangiocarcinoma development that confers an actionable therapeutic vulnerability to AKT inhibition., (©2021 The Authors; Published by the American Association for Cancer Research.)
- Published
- 2021
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