1. A single-cell atlas of chromatin accessibility in the human genome.
- Author
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Zhang K, Hocker JD, Miller M, Hou X, Chiou J, Poirion OB, Qiu Y, Li YE, Gaulton KJ, Wang A, Preissl S, and Ren B
- Subjects
- Adult, Cluster Analysis, Fetus metabolism, Genetic Variation, Genome-Wide Association Study, Humans, Organ Specificity, Phylogeny, Regulatory Sequences, Nucleic Acid genetics, Risk Factors, Chromatin metabolism, Genome, Human, Single-Cell Analysis
- Abstract
Current catalogs of regulatory sequences in the human genome are still incomplete and lack cell type resolution. To profile the activity of gene regulatory elements in diverse cell types and tissues in the human body, we applied single-cell chromatin accessibility assays to 30 adult human tissue types from multiple donors. We integrated these datasets with previous single-cell chromatin accessibility data from 15 fetal tissue types to reveal the status of open chromatin for ∼1.2 million candidate cis-regulatory elements (cCREs) in 222 distinct cell types comprised of >1.3 million nuclei. We used these chromatin accessibility maps to delineate cell-type-specificity of fetal and adult human cCREs and to systematically interpret the noncoding variants associated with complex human traits and diseases. This rich resource provides a foundation for the analysis of gene regulatory programs in human cell types across tissues, life stages, and organ systems., Competing Interests: Declaration of interests B.R. is a shareholder and consultant of Arima Genomics, Inc., and a co-founder of Epigenome Technologies, Inc. K.J.G. is a consultant of Genentech and a shareholder in Vertex Pharmaceuticals. These relationships have been disclosed to and approved by the UCSD Independent Review Committee., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2021
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