1. ERECTA signaling controls Arabidopsis inflorescence architecture through chromatin-mediated activation of PRE1 expression.
- Author
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Cai H, Zhao L, Wang L, Zhang M, Su Z, Cheng Y, Zhao H, and Qin Y
- Subjects
- Arabidopsis anatomy & histology, Arabidopsis genetics, Arabidopsis Proteins genetics, Cell Cycle genetics, Chromatin Assembly and Disassembly physiology, Gene Expression Regulation, Plant, Histones metabolism, Inflorescence metabolism, MAP Kinase Signaling System, Microfilament Proteins genetics, Microfilament Proteins metabolism, Mitogen-Activated Protein Kinase Kinases genetics, Mitogen-Activated Protein Kinase Kinases metabolism, Mitogen-Activated Protein Kinases genetics, Mitogen-Activated Protein Kinases metabolism, Mutation, Nucleosomes metabolism, Protein Serine-Threonine Kinases genetics, Receptors, Cell Surface genetics, Signal Transduction, Transcription Factors genetics, Arabidopsis metabolism, Arabidopsis Proteins metabolism, Chromatin metabolism, Inflorescence anatomy & histology, Protein Serine-Threonine Kinases metabolism, Receptors, Cell Surface metabolism, Transcription Factors metabolism
- Abstract
Flowering plants display a remarkable diversity in inflorescence architecture, and pedicel length is one of the key contributors to this diversity. In Arabidopsis thaliana, the receptor-like kinase ERECTA (ER) mediated signaling pathway plays important roles in regulating inflorescence architecture by promoting cell proliferation. However, the regulating mechanism remains elusive in the pedicel. Genetic interactions between ERECTA signaling and the chromatin remodeling complex SWR1 in the control of inflorescence architecture were studied. Comparative transcriptome analysis was applied to identify downstream components. Chromatin immunoprecipitation and nucleosome occupancy was further investigated. The results indicated that the chromatin remodeler SWR1 coordinates with ERECTA signaling in regulating inflorescence architecture by activating the expression of PRE1 family genes and promoting pedicel elongation. It was found that SWR1 is required for the incorporation of the H2A.Z histone variant into nucleosomes of the whole PRE1 gene family and the ERECTA controlled expression of PRE1 gene family through regulating nucleosome dynamics. We propose that utilization of a chromatin remodeling complex to regulate gene expression is a common theme in developmental control across kingdoms. These findings shed light on the mechanisms through which chromatin remodelers orchestrate complex transcriptional regulation of gene expression in coordination with a developmental cue., (© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust.)
- Published
- 2017
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