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Start Over Author "Foster, Emma R." Topic chromatographic analysis
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1. Capture chromatography with mixed-mode resins: A case study with recombinant human thioredoxin from Escherichia coli.

Author

Ravi, Ayswarya, Foster, Emma R., Perez, Lisa M., and Nikolov, Zivko L.

Subjects
*THIOREDOXIN, *ESCHERICHIA coli, *PROTEIN-ligand interactions, *CHROMATOGRAPHIC analysis, *MOLECULAR dynamics
Abstract

• Active thioredoxin of 90% purity and 95% recovery was purified by HEA HyperCel chromatography. • HEA HyperCel had salt-tolerant and more selective binding than Capto adhere and Toyopearl NH2-750F mixed-mode resins. • Process optimization by high-throughput screening and scale-up performance was evaluated. • Molecular dynamics simulations provide protein-ligand interactions aiding process development. [ABSTRACT FROM AUTHOR]

Published
2020
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