1. Salt‐effect enhanced hollow‐fiber liquid‐phase microextraction of glutathione in human saliva followed by miniaturized capillary electrophoresis with amperometric detection
- Author
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Yaolu Ma, Zhonghui Fang, Jiannong Ye, Xiaoshuang Zhao, Dan Luo, and Qingcui Chu
- Subjects
Saliva ,Liquid Phase Microextraction ,Clinical Biochemistry ,02 engineering and technology ,Sodium Chloride ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,Matrix (chemical analysis) ,chemistry.chemical_compound ,Capillary electrophoresis ,Limit of Detection ,Humans ,Fiber ,Derivatization ,Chromatography ,Chemistry ,010401 analytical chemistry ,Electrophoresis, Capillary ,Reproducibility of Results ,Glutathione ,021001 nanoscience & nanotechnology ,Amperometry ,0104 chemical sciences ,Isoelectric point ,Linear Models ,0210 nano-technology - Abstract
A hollow-fiber liquid-phase microextraction (HF-LPME) method was established for purification and enrichment of glutathione (GSH) in human saliva followed by a miniaturized capillary electrophoresis with amperometric detection system (mini-CE-AD). Based on regulating isoelectric point and increasing salt effect to modify donor phase, HF-LPME could provide high enrichment efficiency for GSH up to 471 times, and the extract was directly injected for mini-CE-AD analysis. The salt-effect enhanced HF-LPME/mini-CE-AD method has been successfully applied to saliva analysis, and acceptable LOD (0.46 ng/mL, S/N = 3) and recoveries (92.7-101.3%) could be obtained in saliva matrix. The sample pretreatment of this developed method was simple and required no derivatization, providing a potential alternative for non-invasive fluid analysis using portable instrument.
- Published
- 2020