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Start Over Topic chromosomal proteins, non-histone
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1. Phosphorylation of nonhistone proteins during premature chromosome condensation in a temperature-sensitive mutant, tsBN2.

Author

Yamashita K, Davis FM, Rao PN, Sekiguchi M, and Nishimoto T

Subjects
Animals, Antibodies, Monoclonal, Antigen-Antibody Reactions, Antigens biosynthesis, Antigens immunology, Cell Line, Chromosomal Proteins, Non-Histone immunology, Collodion, Cricetinae, Electrophoresis, Polyacrylamide Gel, Humans, Mice, Mitosis drug effects, Mutation, Paper, Phosphorylation, Sulfonamides pharmacology, Chromosomal Proteins, Non-Histone metabolism, Chromosomes, Human physiology, Interphase, Temperature
Abstract

In tsBN2 cells, a temperature-sensitive (ts) mutant of the BHK21 cell line, with a ts-defect in its regulatory system for chromosome condensation, antigens that react with mitotic specific mouse monoclonal antibody MPM-2 were produced when premature chromosome condensation (PCC) was induced by a temperature shift. The polypeptides of antigens recognized by MPM-2 in tsBN2 cells with PCC were identical to those of antigens in mitotic cells. These antigens appeared concomitantly with chromosome condensation, which suggests that these mitotic-specific antigens may be related to chromosome condensation. As the production of mitotic-specific antigens was inhibited by W-7, a specific and potent antagonist of calmodulin, calmodulin may function in the mitotic phosphorylation of nonhistone protein.

Published
1985
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2. Phosphorylation of nonhistone proteins during premature chromosome condensation in a temperature-sensitive mutant, tsBN2

Author

Takeharu Nishimoto, Mutsuo Sekiguchi, Frances M. Davis, Katsumi Yamashita, and Potu N. Rao

Subjects
Paper, Calmodulin, Physiology, Chromosomal Proteins, Non-Histone, Mutant, Mitosis, Cell Line, Antigen-Antibody Reactions, Mice, Non-histone protein, Antigen, Cricetinae, Animals, Chromosomes, Human, Humans, Antigens, Phosphorylation, Molecular Biology, Interphase, Sulfonamides, biology, Temperature, Antibodies, Monoclonal, Collodion, Cell Biology, General Medicine, Temperature-sensitive mutant, Molecular biology, Premature chromosome condensation, Mutation, biology.protein, Electrophoresis, Polyacrylamide Gel
Abstract

In tsBN2 cells, a temperature-sensitive (ts) mutant of the BHK21 cell line, with a ts-defect in its regulatory system for chromosome condensation, antigens that react with mitotic specific mouse monoclonal antibody MPM-2 were produced when premature chromosome condensation (PCC) was induced by a temperature shift. The polypeptides of antigens recognized by MPM-2 in tsBN2 cells with PCC were identical to those of antigens in mitotic cells. These antigens appeared concomitantly with chro-mosome condensation, which suggests that these mitotic-specific antigens may be related to chromosome condensation. As the production of mitotic-specific antigens was inhibited by W-7, a specific and potent antagonist of calmodulin, calmodulin may function in the mitotic phosphorylation of nonhistone protein.

Published
1985