Search

Your search keyword '"L. De Ponti"' showing total 3 results
Start Over Author "L. De Ponti" Topic chromosome mapping
3 results on '"L. De Ponti"'

1. Sex-, tissue-, and stage-specific expression of a vitelline membrane protein gene from region 32 of the second chromosome of Drosophila melanogaster.

Author

Gigliotti S, Graziani F, De Ponti L, Rafti F, Manzi A, Lavorgna G, Gargiulo G, and Malva C

Subjects
Amino Acid Sequence, Animals, Base Sequence, Blotting, Northern, Drosophila melanogaster growth & development, Female, Male, Molecular Sequence Data, Nucleic Acid Hybridization, Restriction Mapping, Sex Factors, Transcription, Genetic, Chromosome Mapping, Drosophila melanogaster genetics, Egg Proteins genetics, Gene Expression Regulation, Genes
Abstract

This study isolated cDNA clones from egg-chamber and adult female Drosophila cDNA libraries using as probe a DNA fragment from a 200-kb "chromosome walk" in region 32E of the second chromosome of D. melanogaster. The present authors believe that these clones correspond to a new vitelline membrane protein (VMP) gene because 1) cDNA clones in Northern blots identify a transcript expressed in a tissue- and stage-specific manner: stage 10 egg-chambers; 2) the sequence of cDNAs and of the genomic subclone shows homology with the other VMP genes that have been identified to date; 3) the amino acid composition of the translational product has the high content of proline and alanine characteristic of VMPs. Two aspects emerging from this study are worth stressing: 1) the presence of a hydrophobic domain that is highly conserved in all the VMP genes; and 2) the particularly narrow period of expression of the isolated gene, which could be related to the mechanism of vitelline membrane assembly.

Published
1989
Full Text
View/download PDF

2. Isolation and characterization of the CRIPTO autosomal gene and its X-linked related sequence

Author

R, Dono, N, Montuori, M, Rocchi, L, De Ponti-Zilli, A, Ciccodicola, and M G, Persico

Subjects
Membrane Glycoproteins, X Chromosome, Base Sequence, Epidermal Growth Factor, Molecular Sequence Data, Chromosome Mapping, GPI-Linked Proteins, Cell Line, Neoplasm Proteins, Blotting, Southern, Mice, Multigene Family, Mutation, Tumor Cells, Cultured, Animals, Humans, Intercellular Signaling Peptides and Proteins, Amino Acid Sequence, Chromosomes, Human, Pair 3, Growth Substances, Research Article
Abstract

We have previously reported on the identification of a cDNA clone encoding a novel human growth factor, named "CRIPTO," that is abundantly expressed in undifferentiated human NTERA-2 clone D1 (NT2/D1) and mouse (F9) teratocarcinoma cells. We now report the organization and nucleotide sequence of two related genomic sequences. One (CR-1) corresponds to the structural gene encoding the human CRIPTO protein expressed in the undifferentiated human teratocarcinoma cells, and the other (CR-3) corresponds to a complete copy of the mRNA containing seven base substitutions in the coding region representing both silent and replacement substitutions. The 440 bp 5' to the CAP site of CR-1 are preserved in CR-3. CR-1 maps to chromosome 3, and CR-3 maps to Xq21-q22. Southern blot analysis reveals that multiple CRIPTO-related DNA sequences are present in the human as well as in the mouse genome.

Published
1991

3. Sex-, tissue-, and stage-specific expression of a vitelline membrane protein gene from region 32 of the second chromosome of Drosophila melanogaster

Author

G. Lavorgna, Andrea Manzi, L. De Ponti, Carla Malva, Franco Graziani, G. Gargiulo, F. Rafti, and S. Gigliotti

Subjects
Male, Transcription, Genetic, Molecular Sequence Data, Restriction Mapping, Vitelline membrane, Biology, Homology (biology), Gene product, Sex Factors, Complementary DNA, Genetics, Gene family, Animals, Amino Acid Sequence, Gene, Base Sequence, cDNA library, Egg Proteins, Chromosome Mapping, Nucleic Acid Hybridization, Cell Biology, biology.organism_classification, Blotting, Northern, Molecular biology, Drosophila melanogaster, Gene Expression Regulation, Genes, Female, Developmental Biology
Abstract

This study isolated cDNA clones from egg-chamber and adult female Drosophila cDNA libraries using as probe a DNA fragment from a 200-kb “chromosome walk” in region 32E of the second chromosome of D. melanogaster. The present authors believe that these clones correspond to a new vitelline membrane protein (VMP) gene because (1) cDNA clones in Northern blots identify a transcript expressed in a tissue- and stage-specific manner: stage 10 egg-chambers; (2) the sequence of cDNAs and of the genomic subclone shows homology with the other VMP genes that have been identified to date; (3) the amino acid composition of the translational product has the high content of proline and alanine characteristic of VMPs. Two aspects emerging from this study are worth stressing: (1) the presence of a hydrophobic domain that is highly conserved in all the VMP genes; and (2) the particularly narrow period of expression of the isolated gene, which could be related to the mechanism of vitelline membrane assembly.

Published
1989

Catalog

Books, media, physical & digital resources
See catalog results