1. [Alteration of cholera toxin biosynthesis in Vibrio cholerae 01 as a result of temperate phage 139 integration into bacterial chromosome].
- Author
-
Eroshenko GA and Smirnova NI
- Subjects
- Adhesins, Bacterial genetics, Adhesins, Bacterial metabolism, Bacterial Outer Membrane Proteins genetics, Bacterial Outer Membrane Proteins metabolism, Bacterial Proteins genetics, Bacterial Proteins metabolism, Cholera Toxin genetics, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Gene Dosage, Lysogeny, Transcription Factors genetics, Transcription Factors metabolism, Bacteriophages physiology, Cholera Toxin biosynthesis, Chromosomes, Bacterial, Vibrio cholerae genetics, Vibrio cholerae metabolism
- Abstract
Infection of V. cholerae 01 (classical and eltor biovars) cells with the temperate cholera phage 139 derived from V. cholerae serogroup 0139 followed by integration of the phage genome into the bacterial chromosome significantly increased the production of cholera toxin, the main virulence factor. The level of toxin biosynthesis in the lysogenic V. cholerae classical strain increased 3-fold and that in V. eltor thirty times in comparison with the parental strains. Increased production of cholera toxin was not associated with an increase in the number of copies of genes involved in its biosynthesis but seemed to be due to changes in toxinogenesis regulation.
- Published
- 2002