1. Retrograde signaling by a mtDNA-encoded non-coding RNA preserves mitochondrial bioenergetics.
- Author
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Blumental-Perry A, Jobava R, Bederman I, Degar AJ, Kenche H, Guan BJ, Pandit K, Perry NA, Molyneaux ND, Wu J, Prendergas E, Ye ZW, Zhang J, Nelson CE, Ahangari F, Krokowski D, Guttentag SH, Linden PA, Townsend DM, Miron A, Kang MJ, Kaminski N, Perry Y, and Hatzoglou M
- Subjects
- Alveolar Epithelial Cells drug effects, Alveolar Epithelial Cells metabolism, Animals, Cell Line, Cell Nucleus genetics, Electron Transport genetics, Female, Gene Expression Regulation drug effects, Mice, Inbred C57BL, MicroRNAs genetics, Mitochondria drug effects, Mitochondria metabolism, RNA, Untranslated drug effects, RNA, Untranslated genetics, Signal Transduction, Cigarette Smoking adverse effects, DNA, Mitochondrial genetics, Energy Metabolism genetics, Mitochondria genetics, RNA, Untranslated metabolism
- Abstract
Alveolar epithelial type II (AETII) cells are important for lung epithelium maintenance and function. We demonstrate that AETII cells from mouse lungs exposed to cigarette smoke (CS) increase the levels of the mitochondria-encoded non-coding RNA, mito-RNA-805, generated by the control region of the mitochondrial genome. The protective effects of mito-ncR-805 are associated with positive regulation of mitochondrial energy metabolism, and respiration. Levels of mito-ncR-805 do not relate to steady-state transcription or replication of the mitochondrial genome. Instead, CS-exposure causes the redistribution of mito-ncR-805 from mitochondria to the nucleus, which correlated with the increased expression of nuclear-encoded genes involved in mitochondrial function. These studies reveal an unrecognized mitochondria stress associated retrograde signaling, and put forward the idea that mito-ncRNA-805 represents a subtype of small non coding RNAs that are regulated in a tissue- or cell-type specific manner to protect cells under physiological stress.
- Published
- 2020
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