1. Densazalin, a New Cytotoxic Diazatricyclic Alkaloid from the Marine Sponge Haliclona densaspicula.
- Author
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Hwang, Buyng Su, Jeong, Yong Tae, Lee, Sangbum, Jeong, Eun Ju, and Rho, Jung-Rae
- Subjects
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SPONGES (Invertebrates) , *NUCLEAR magnetic resonance , *MASS spectrometry , *ALKALOIDS , *CIRCULAR dichroism , *CELL lines - Abstract
Densazalin, a polycyclic alkaloid, was isolated from the marine sponge Haliclona densaspicula collected in Korea. The complete structure of the compound was determined by spectroscopic methods, including 1D and 2D nuclear magnetic resonance techniques, high-resolution mass spectrometry, and comparison of the calculated and measured electronic circular dichroism spectra. Densazalin possesses a unique 5,11-diazatricyclo[7.3.1.02,7]tridecan-2,4,6-triene moiety, which is connected by two linear carbon chains. This compound was derived from the biogenetic precursor bis-1,3-dialkylpyridnium. Densazalin exhibited cytotoxic activity on two human tumor cell lines (AGS and HepG2) in the Cell Counting Kit-8 (CCK-8) bioassay, with IC50 values ranging from 15.5 to 18.4 μM. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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