1. Increased intracellular Ca 2+ decreases cisplatin resistance by regulating iNOS expression in human ovarian cancer cells.
- Author
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Yu Y, Xie Q, Liu W, Guo Y, Xu N, Xu L, Liu S, Li S, Xu Y, and Sun L
- Subjects
- Amino Acid Sequence, Cell Survival drug effects, Cell Survival physiology, Cisplatin pharmacology, Dose-Response Relationship, Drug, Drug Resistance, Neoplasm drug effects, Female, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Humans, Intracellular Fluid drug effects, Nitric Oxide Synthase Type II genetics, Ovarian Neoplasms genetics, Calcium metabolism, Cisplatin metabolism, Drug Resistance, Neoplasm physiology, Intracellular Fluid metabolism, Nitric Oxide Synthase Type II biosynthesis, Ovarian Neoplasms metabolism
- Abstract
Previous studies have reported that intracellular Ca
2+ signals and inducible nitric oxide synthase (iNOS) are involved in cell apoptosis. However, the role of iNOS in cisplatin resistance in ovarian cancer remains unclear. Here, we demonstrate that SKOV3/DDP ovarian cancer cells were more resistant to cisplatin than were SKOV3 ovarian cancer cells. The expression of intracellular Ca2+ and iNOS was more strongly induced by cisplatin in SKOV3 cells than in SKOV3/DDP cells. TAT-conjugated IP3R-derived peptide (TAT-IDPS ) increased cisplatin-induced iNOS expression and apoptosis in SKOV3/DDP cells. 2-Aminoethoxydiphenyl borate (2-APB) decreased cisplatin-induced iNOS expression and apoptosis in SKOV3 cells. Thus, iNOS induction may be a valuable strategy for improving the anti-tumor efficacy of cisplatin in ovarian cancer., (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)- Published
- 2017
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