1. Diversity of aromatic ring-hydroxylating dioxygenase gene in Citrobacter.
- Author
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Selvakumaran S, Kapley A, Kashyap SM, Daginawala HF, Kalia VC, and Purohit HJ
- Subjects
- Base Sequence, Citrobacter genetics, Cluster Analysis, DNA Primers genetics, Dioxygenases metabolism, Hydroxylation, Molecular Sequence Data, Sequence Analysis, DNA, Sequence Homology, Species Specificity, Spectrophotometry, Ultraviolet, Citrobacter enzymology, Dioxygenases genetics, Environmental Restoration and Remediation methods, Genetic Variation, Hydrocarbons, Aromatic metabolism, Phylogeny
- Abstract
Genetic and functional diversity of Citrobacter spp. for their abilities to degrade aromatic compounds was evaluated to develop mixed cultures or a consortium for bioremediation technology. Thirty Citrobacter strains isolated from various effluent treatment plants were found to degrade a range of aromatic compounds: phenol, benzoate, hydroxy benzoic acid and biotransform mono-chlorophenols and di-chlorophenol within 24 to 48 h of incubation at 30 °C. Sequence similarity and phylogeny of the ARHD gene transcripts (730 nucleotides) depicted their diversity within 9 Citrobacter strains: HPC255, HPC369, HPC560, HPC570, HPC784, HPC1196, HPC1216, HPC1276 and HPC1299. Here, the degree of associations varied up to 84% with (i) ARHD α-sub unit (SU), (ii) LSU of Phenylpropionate dioxygenase (PDO), (iii) Phenol hydroxylase α-SU, (iv) Benzoate 1,2-dioxygenase, α-SU, (v) Naphthalene dioxygenase LSU, etc. This study has provided basic information, which can be used to develop a consortium of bacteria with mutually beneficial characteristics., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
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