19 results on '"Dupont, Sam"'
Search Results
2. On the preconditions for large-scale collective action.
- Author
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Jagers SC, Harring N, Löfgren Å, Sjöstedt M, Alpizar F, Brülde B, Langlet D, Nilsson A, Almroth BC, Dupont S, and Steffen W
- Subjects
- Biodiversity, Climate Change, Cooperative Behavior
- Abstract
The phenomenon of collective action and the origin of collective action problems have been extensively and systematically studied in the social sciences. Yet, while we have substantial knowledge about the factors promoting collective action at the local level, we know far less about how these insights travel to large-scale collective action problems. Such problems, however, are at the heart of humanity's most pressing challenges, including climate change, large-scale natural resource depletion, biodiversity loss, nuclear proliferation, antibiotic resistance due to overconsumption of antibiotics, and pollution. In this paper, we suggest an analytical framework that captures the theoretical understanding of preconditions for large-scale collective action. This analytical framework aims at supporting future empirical analyses of how to cope with and overcome larger-scale collective action problems. More specifically, we (i) define and describe the main characteristics of a large-scale collective action problem and (ii) explain why voluntary and, in particular, spontaneous large-scale collective action among individual actors becomes more improbable as the collective action problem becomes larger, thus demanding interventions by an external authority (a third party) for such action to be generated. Based on this, we (iii) outline an analytical framework that illustrates the connection between third-party interventions and large-scale collective action. We conclude by suggesting avenues for future research.
- Published
- 2020
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3. Experimental strategies to assess the biological ramifications of multiple drivers of global ocean change-A review.
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Boyd PW, Collins S, Dupont S, Fabricius K, Gattuso JP, Havenhand J, Hutchins DA, Riebesell U, Rintoul MS, Vichi M, Biswas H, Ciotti A, Gao K, Gehlen M, Hurd CL, Kurihara H, McGraw CM, Navarro JM, Nilsson GE, Passow U, and Pörtner HO
- Subjects
- Animals, Biological Evolution, Climate Change, Environmental Monitoring methods, Oceans and Seas
- Abstract
Marine life is controlled by multiple physical and chemical drivers and by diverse ecological processes. Many of these oceanic properties are being altered by climate change and other anthropogenic pressures. Hence, identifying the influences of multifaceted ocean change, from local to global scales, is a complex task. To guide policy-making and make projections of the future of the marine biosphere, it is essential to understand biological responses at physiological, evolutionary and ecological levels. Here, we contrast and compare different approaches to multiple driver experiments that aim to elucidate biological responses to a complex matrix of ocean global change. We present the benefits and the challenges of each approach with a focus on marine research, and guidelines to navigate through these different categories to help identify strategies that might best address research questions in fundamental physiology, experimental evolutionary biology and community ecology. Our review reveals that the field of multiple driver research is being pulled in complementary directions: the need for reductionist approaches to obtain process-oriented, mechanistic understanding and a requirement to quantify responses to projected future scenarios of ocean change. We conclude the review with recommendations on how best to align different experimental approaches to contribute fundamental information needed for science-based policy formulation., (© 2018 John Wiley & Sons Ltd.)
- Published
- 2018
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4. Adjustments of molecular key components of branchial ion and pH regulation in Atlantic cod (Gadus morhua) in response to ocean acidification and warming.
- Author
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Michael K, Kreiss CM, Hu MY, Koschnick N, Bickmeyer U, Dupont S, Pörtner HO, and Lucassen M
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- Adenosine Triphosphatases genetics, Adenosine Triphosphatases metabolism, Animals, Biological Transport, Carbon Dioxide chemistry, Female, Fish Proteins genetics, Fish Proteins metabolism, Gene Expression Regulation, Hydrogen-Ion Concentration, Male, Proton-Translocating ATPases genetics, Proton-Translocating ATPases metabolism, Temperature, Climate Change, Gadus morhua genetics, Gadus morhua metabolism, Seawater chemistry
- Abstract
Marine teleost fish sustain compensation of extracellular pH after exposure to hypercapnia by means of efficient ion and acid-base regulation. Elevated rates of ion and acid-base regulation under hypercapnia may be stimulated further by elevated temperature. Here, we characterized the regulation of transepithelial ion transporters (NKCC1, NBC1, SLC26A6, NHE1 and 2) and ATPases (Na(+)/K(+) ATPase and V-type H(+) ATPase) in gills of Atlantic cod (Gadus morhua) after 4 weeks of exposure to ambient and future PCO2 levels (550 μatm, 1200 μatm, 2200 μatm) at optimum (10 °C) and summer maximum temperature (18 °C), respectively. Gene expression of most branchial ion transporters revealed temperature- and dose-dependent responses to elevated PCO2. Transcriptional regulation resulted in stable protein expression at 10 °C, whereas expression of most transport proteins increased at medium PCO2 and 18 °C. mRNA and protein expression of distinct ion transport proteins were closely co-regulated, substantiating cellular functional relationships. Na(+)/K(+) ATPase capacities were PCO2 independent, but increased with acclimation temperature, whereas H(+) ATPase capacities were thermally compensated but decreased at medium PCO2 and 10 °C. When functional capacities of branchial ATPases were compared with mitochondrial F1Fo ATP-synthase strong correlations of F1Fo ATP-synthase and ATPase capacities generally indicate close coordination of branchial aerobic ATP demand and supply. Our data indicate physiological plasticity in the gills of cod to adjust to a warming, acidifying ocean within limits. In light of the interacting and non-linear, dose-dependent effects of both climate factors the role of these mechanisms in shaping resilience under climate change remains to be explored., (Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2016
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5. Ocean acidification through the lens of ecological theory.
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Gaylord B, Kroeker KJ, Sunday JM, Anderson KM, Barry JP, Brown NE, Connell SD, Dupont S, Fabricius KE, Hall-Spencer JH, Klinger T, Milazzo M, Munday PL, Russell BD, Sanford E, Schreiber SJ, Thiyagarajan V, Vaughan ML, Widdicombe S, and Harley CD
- Subjects
- Acclimatization, Animals, Models, Biological, Climate Change, Ecology, Ecosystem, Oceans and Seas, Seawater chemistry
- Abstract
Ocean acidification, chemical changes to the carbonate system of seawater, is emerging as a key environmental challenge accompanying global warming and other human-induced perturbations. Considerable research seeks to define the scope and character of potential outcomes from this phenomenon, but a crucial impediment persists. Ecological theory, despite its power and utility, has been only peripherally applied to the problem. Here we sketch in broad strokes several areas where fundamental principles of ecology have the capacity to generate insight into ocean acidification's consequences. We focus on conceptual models that, when considered in the context of acidification, yield explicit predictions regarding a spectrum of population- and community-level effects, from narrowing of species ranges and shifts in patterns of demographic connectivity, to modified consumer-resource relationships, to ascendance of weedy taxa and loss of species diversity. Although our coverage represents only a small fraction of the breadth of possible insights achievable from the application of theory, our hope is that this initial foray will spur expanded efforts to blend experiments with theoretical approaches. The result promises to be a deeper and more nuanced understanding of ocean acidification'and the ecological changes it portends.
- Published
- 2015
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6. Response to 'How and how not to investigate the oxygen and capacity limitation of thermal tolerance (OCLTT) and aerobic scope—remarks on the article by Gräns et al.'.
- Author
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Jutfelt F, Gräns A, Jönsson E, Wiklander K, Seth H, Olsson C, Dupont S, Ortega-Martinez O, Sundell K, Axelsson M, and Sandblom E
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- Animals, Climate Change, Flounder physiology
- Published
- 2014
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7. Aerobic scope fails to explain the detrimental effects on growth resulting from warming and elevated CO2 in Atlantic halibut.
- Author
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Gräns A, Jutfelt F, Sandblom E, Jönsson E, Wiklander K, Seth H, Olsson C, Dupont S, Ortega-Martinez O, Einarsdottir I, Björnsson BT, Sundell K, and Axelsson M
- Subjects
- Animals, Body Temperature Regulation, Carbon Dioxide metabolism, Flounder growth & development, Hot Temperature, Hydrogen-Ion Concentration, Oxygen metabolism, Climate Change, Flounder physiology
- Abstract
As a consequence of increasing atmospheric CO2, the world's oceans are becoming warmer and more acidic. Whilst the ecological effects of these changes are poorly understood, it has been suggested that fish performance including growth will be reduced mainly as a result of limitations in oxygen transport capacity. Contrary to the predictions given by the oxygen- and capacity-limited thermal tolerance hypothesis, we show that aerobic scope and cardiac performance of Atlantic halibut (Hippoglossus hippoglossus) increase following 14-16 weeks exposure to elevated temperatures and even more so in combination with CO2-acidified seawater. However, the increase does not translate into improved growth, demonstrating that oxygen uptake is not the limiting factor for growth performance at high temperatures. Instead, long-term exposure to CO2-acidified seawater reduces growth at temperatures that are frequently encountered by this species in nature, indicating that elevated atmospheric CO2 levels may have serious implications on fish populations in the future.
- Published
- 2014
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8. Genomic Characterization of the Evolutionary Potential of the Sea Urchin Strongylocentrotus droebachiensis Facing Ocean Acidification
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Runcie, Daniel E, Dorey, Narimane, Garfield, David A, Stumpp, Meike, Dupont, Sam, and Wray, Gregory A
- Subjects
Human Genome ,Biotechnology ,Genetics ,Generic health relevance ,Life Below Water ,Adaptation ,Physiological ,Animals ,Carbon Dioxide ,Climate Change ,Evolution ,Molecular ,Genomics ,Oceans and Seas ,Seawater ,Strongylocentrotus ,System genetics ,climate change ,genetic variation ,plasticity ,RNAseq ,gene set variation analysis ,Biochemistry and Cell Biology ,Evolutionary Biology ,Developmental Biology - Abstract
Ocean acidification (OA) is increasing due to anthropogenic CO2 emissions and poses a threat to marine species and communities worldwide. To better project the effects of acidification on organisms' health and persistence, an understanding is needed of the 1) mechanisms underlying developmental and physiological tolerance and 2) potential populations have for rapid evolutionary adaptation. This is especially challenging in nonmodel species where targeted assays of metabolism and stress physiology may not be available or economical for large-scale assessments of genetic constraints. We used mRNA sequencing and a quantitative genetics breeding design to study mechanisms underlying genetic variability and tolerance to decreased seawater pH (-0.4 pH units) in larvae of the sea urchin Strongylocentrotus droebachiensis. We used a gene ontology-based approach to integrate expression profiles into indirect measures of cellular and biochemical traits underlying variation in larval performance (i.e., growth rates). Molecular responses to OA were complex, involving changes to several functions such as growth rates, cell division, metabolism, and immune activities. Surprisingly, the magnitude of pH effects on molecular traits tended to be small relative to variation attributable to segregating functional genetic variation in this species. We discuss how the application of transcriptomics and quantitative genetics approaches across diverse species can enrich our understanding of the biological impacts of climate change.
- Published
- 2016
9. The Carbon Footprint as a Mediating Tool in Students’ Online Reasoning about Climate Change
- Author
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Fauville, Géraldine, Lantz-Andersson, Annika, Mäkitalo, Åsa, Dupont, Sam, Säljö, Roger, Livingstone, D. W., Series Editor, Guile, David, Series Editor, Erstad, Ola, editor, Kumpulainen, Kristiina, editor, Mäkitalo, Åsa, editor, Schrøder, Kim Christian, editor, Pruulmann-Vengerfeldt, Pille, editor, and Jóhannsdóttir, Thuridur, editor
- Published
- 2016
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10. Virtual Ocean Acidification Laboratory as an Efficient Educational Tool to Address Climate Change Issues
- Author
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Fauville, Géraldine, Hodin, Jason, Dupont, Sam, Miller, Pam, Haws, Julie, Thorndyke, Michael, Epel, David, and Leal Filho, Walter, editor
- Published
- 2011
- Full Text
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11. Near-future level of CO₂-driven ocean acidification radically affects larval survival and development in the brittlestar Ophiothrix fragilis
- Author
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Dupont, Sam, Havenhand, Jon, Thorndyke, William, Peck, Lloyd, and Thorndyke, Michael
- Published
- 2008
12. Sex-specific digestive performance of mussels exposed to warming and starvation.
- Author
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Yueyong Shang, Shuaishuai Wei, Xueqing Chang, Yiran Mao, Dupont, Sam, Kar-Hei Fang, James, Menghong Hu, and Youji Wang
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MUSSELS ,DIGESTIVE enzymes ,CLIMATE change ,STARVATION ,PRINCIPAL components analysis - Abstract
As global climate change has dramatically impacted the ocean, severe temperature elevation and a decline in primary productivity has frequently occurred, which has affected the structure of coastal biomes. In this study, the sex-specific responses to temperature change and food availability in mussels were determined in terms of digestive performance. The thick-shelled mussels Mytilus coruscus (male and female) were exposed to different temperature and nutritional conditions for 30 days. The results showed that the digestive enzymes of mussels were significantly affected by temperature, food, sex, and their interactions. High temperature (30°C) and starvation significantly decreased amylase, lysozyme, and pepsase activities of female mussels, while trypsin and trehalase did not change significantly at the experimental end. The activity of amylase, trypsin, and trehalase was significantly reduced in males at high temperature (30°C) under starvation treatment, but high temperature (30°C) elevated pepsase. Unsurprisingly, starvation caused the reduction of lysozyme and pepsase under 25°C in males. Amylase, lipase, and trehalase were higher in female mussels compared with males, while the enzymatic activities of lysozyme, pepsase, and trypsin were higher in male mussels than females. Principal component analysis showed that different enzyme activity indexes were separated in male and female mussels, indicating that male and female mussels exhibited significantly different digestive abilities under temperature and food condition change. The study clarified sex-specific response difference in mussel digestive enzymes under warming and starvation and provided guidance for the development of mussel aquaculture (high temperature management and feeding strategy) under changing marine environments. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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13. Attuning to a changing ocean.
- Author
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Stenseth, Nils Chr., Payne, Mark R., Bonsdorff, Erik, Dankel, Dorothy J., Durant, Joël M., Anderson, Leif G., Armstron, Claire W., Blenckner, Thorsten, Brakstad, Ailin, Dupont, Sam, Eikeset, Anne M., Goksøyr, Anders, ´onsson, Steingr´ımur J, Kuparinen, Anna, Våge, Kjetil, Österblom, Henrik, and Paasche, Øyvind
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OCEAN ,POLICY sciences ,DEVELOPING countries ,SCIENTISTS ,COOPERATIVE research - Abstract
The ocean is a lifeline for human existence, but current practices risk severely undermining ocean sustainability. Present and future social−ecological challenges necessitate the maintenance and development of knowledge and action by stimulating collaboration among scientists and between science, policy, and practice. Here we explore not only how such collaborations have developed in the Nordic countries and adjacent seas but also how knowledge from these regions contributes to an understanding of how to obtain a sustainable ocean. Our collective experience may be summarized in three points: 1) In the absence of long-term observations, decision-making is subject to high risk arising from natural variability; 2) in the absence of established scientific organizations, advice to stakeholders often relies on a few advisors, making them prone to biased perceptions; and 3) in the absence of trust between policy makers and the science community, attuning to a changing ocean will be subject to arbitrary decision-making with unforeseen and negative ramifications. Underpinning these observations, we show that collaboration across scientific disciplines and stakeholders and between nations is a necessary condition for appropriate actions. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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14. Contrasting physiological responses to future ocean acidification among Arctic copepod populations.
- Author
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Thor, Peter, Bailey, Allison, Dupont, Sam, Calosi, Piero, Søreide, Janne E., De Wit, Pierre, Guscelli, Ella, Loubet‐Sartrou, Lea, Deichmann, Ida M., Candee, Martin M., Svensen, Camilla, King, Andrew L., and Bellerby, Richard G. J.
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OCEAN acidification ,WATER acidification ,COPEPODA ,CLIMATE change ,ECOSYSTEMS - Abstract
Widespread ocean acidification ( OA) is modifying the chemistry of the global ocean, and the Arctic is recognized as the region where the changes will progress at the fastest rate. Moreover, Arctic species show lower capacity for cellular homeostasis and acid-base regulation rendering them particularly vulnerable to OA. In the present study, we found physiological differences in OA response across geographically separated populations of the keystone Arctic copepod Calanus glacialis. In copepodites stage CIV, measured reaction norms of ingestion rate and metabolic rate showed severe reductions in ingestion and increased metabolic expenses in two populations from Svalbard (Kongsfjord and Billefjord) whereas no effects were observed in a population from the Disko Bay, West Greenland. At pH
T 7.87, which has been predicted for the Svalbard west coast by year 2100, these changes resulted in reductions in scope for growth of 19% in the Kongsfjord and a staggering 50% in the Billefjord. Interestingly, these effects were not observed in stage CV copepodites from any of the three locations. It seems that CVs may be more tolerant to OA perhaps due to a general physiological reorganization to meet low intracellular pH during hibernation. Needless to say, the observed changes in the CIV stage will have serious implications for the C. glacialis population health status and growth around Svalbard. However, OA tolerant populations such as the one in the Disko Bay could help to alleviate severe effects in C. glacialis as a species. [ABSTRACT FROM AUTHOR]- Published
- 2018
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15. Physiological and gene expression responses of the mussel Mytilus galloprovincialis to low pH and low dissolved oxygen.
- Author
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Belivermiş, Murat, Kılıç, Önder, Gezginci-Oktayoglu, Selda, Sezer, Narin, Demiralp, Selcan, Şahin, Berna, and Dupont, Sam
- Subjects
MYTILUS galloprovincialis ,GENE expression ,OXYGEN consumption ,MUSSELS ,CLIMATE change ,OCEAN acidification - Abstract
The prevalence and frequency of hypoxia events have increased worldwide over the past decade as a consequence of global climate change and coastal biological oxygen depletions. On the other hand, anthropogenic emissions of CO 2 and consequent accumulation in the sea surface result in a perturbation of the seawater carbonate system, including a decrease in pH, known as ocean acidification. While the effect of decreases in pH and dissolved oxygen (DO) concentration is better understood, their combined effects are still poorly resolved. Here, we exposed adult mussels (Mytilus galloprovincialis) to two pHs (8.27 and 7.63) and DO concentrations (7.65 and 2.75 mg L
−1 ) over 17 days in a full-factorial design. These levels correspond to extremes of the present natural variability and are relevant in the context of ocean acidification and hypoxia. No mortality was observed during the experiment. However, sublethal effects were observed for clearance and oxygen consumption rates, as well as total haemocytes count and haemocytes viability and gene expression in mussels exposed to the combination of low pH and low DO. Respiration and excretion rates were not significantly impacted by low pH and DO, alone or in combination. Overall, low pH alone led to a decrease in all tested physiological parameters while low DO alone led to a decline in clearance rate, haemocyte parameters and an increase in carbohydrate content. Both parameters led to up- or down-regulation of most of the selected genes. Not surprisingly, the combined effect of low pH and low DO could not be predicted by a simple arithmetic additive response at the effect level, highlighting more complex and non-linear effects. [Display omitted] • Low pH and DO, alone or in combination, affected physiology and gene expression in mussels. • Low pH alone led to a decrease of all tested physiological parameters. • Low DO decreased the clearance rate, modulated haemocytes parameters, increased carbohydrates levels. • The combined effect of low pH and low DO could not be predicted by a simple arithmetic additive response. [ABSTRACT FROM AUTHOR]- Published
- 2023
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16. Near Future Ocean Acidification Increases Growth Rate of the Lecithotrophie Larvae and Juveniles of the Sea Star Crossaster papposus.
- Author
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DUPONT, SAM, LUNDVEI, BENGT, and THORNDYKE, MIKE
- Subjects
OCEAN acidification ,ECHINODERMATA ,WATER acidification ,STARFISHES ,CLIMATE change ,EFFECT of climate on aquaculture - Abstract
The article presents a study on the effects of ocean acidification (OA) on the echinoderm sea star crossaster papposus. Echinoderms are regarded as the most abundant and ecologically successful marine groups. The study showed that lecithotrophic echinoderm larvae and juveniles are affected by ocean acidification. The importance of considering different life-history strategies when evaluating the impacts of climate change is emphasized.
- Published
- 2010
- Full Text
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17. Growth and survival in a changing environment: Combined effects of moderate hypoxia and low pH on juvenile bivalve Macoma balthica.
- Author
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Jansson, Anna, Norkko, Joanna, Dupont, Sam, and Norkko, Alf
- Subjects
- *
CLIMATE change , *HYPOXIA (Water) , *PH effect , *MACOMA baltica - Abstract
Baltic Sea species live in a complex, variable environment characterized by highly fluctuating hydrology, including large seasonal and diel pH variations. For decades, oxygen deficiency caused by anthropogenic eutrophication has affected the Baltic Sea, and large areas of the seafloor are permanently hypoxic resulting in severely degraded benthic communities. Species living in this system are thus potentially tolerant and adapted to this fluctuating environment, but also vulnerable as illustrated by high mortality of benthic species as a result of hypoxia. In the future, the frequency and extent of regularly co-occurring low oxygen and low pH levels will likely increase with on-going climate change. A key species in the Baltic Sea soft-bottom communities, the bivalve Macoma balthica (L.), experiences such conditions throughout its life-cycle, and therefore serves as a good model organism for studying the combined effects of oxygen and pH conditions. To study the response of M. balthica to multiple changes occurring in the benthic environment, we conducted an experiment to investigate the survival and shell growth of newly settled juveniles simultaneously exposed to two pH levels (7.85 and 7.35) and two oxygen levels (8.5 and 3.0 mg/l) for 29 days in a fully factorial design. Survival was high in all treatments (> 60%), but significantly higher in the two low oxygen treatments (> 70%). Although positive growth was observed in all treatments, pH and oxygen as well as their interaction significantly affected relative growth. The highest growth was observed in the “low O 2 /high pH” treatment, which was 2.4 times higher than in both treatments with high oxygen. Although the mechanism for these differences remains unknown, hypoxia-induced metabolic depression likely plays a role. Our results highlight the need to know more about the occurrence and performance of benthic species regularly exposed to changing conditions, and of the range and conditions encountered in situ. With the predicted future negative changes in oxygen availability as well as pH, the adaptive responses of benthic species to multiple stressors will be critical in understanding ecosystem dynamics in the face of change. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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18. Evolution in an acidifying ocean.
- Author
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Sunday, Jennifer M., Calosi, Piero, Dupont, Sam, Munday, Philip L., Stillman, Jonathon H., and Reusch, Thorsten B.H.
- Subjects
- *
EVOLUTIONARY theories , *BIOLOGICAL evolution , *OCEAN acidification , *BIOLOGICAL variation , *ENVIRONMENTAL sciences , *POPULATION biology , *ECOLOGICAL research - Abstract
Highlights: [•] We summarize tools for assessing evolutionary potential under ocean acidification. [•] We review studies of past adaptation, genetic variation, and experimental evolution. [•] We highlight progress and challenges and recommend future research directions. [•] Longer-term experiments that focus on fitness-related responses are recommended. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
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19. Temperature modulates the response of the thermophilous sea urchin Arbacia lixula early life stages to CO2-driven acidification.
- Author
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Gianguzza, Paola, Visconti, Giulia, Gianguzza, Fabrizio, Vizzini, Salvatrice, Sarà, Gianluca, and Dupont, Sam
- Subjects
- *
OCEAN temperature , *SEA urchins , *ARBACIA , *LIFE sciences , *CARBON dioxide in seawater , *OCEAN acidification - Abstract
The increasing abundances of the thermophilous black sea urchin Arbacia lixula in the Mediterranean Sea are attributed to the Western Mediterranean warming. However, few data are available on the potential impact of this warming on A. lixula in combination with other global stressors such as ocean acidification. The aim of this study is to investigate the interactive effects of increased temperature and of decreased pH on fertilization and early development of A. lixula. This was tested using a fully crossed design with four temperatures (20, 24, 26 and 27 °C) and two pH levels (pHNBS 8.2 and 7.9). Temperature and pH had no significant effect on fertilization and larval survival (2d) for temperature <27 °C. At 27 °C, the fertilization success was very low (<1%) and all larvae died within 2d. Both temperature and pH had effects on the developmental dynamics. Temperature appeared to modulate the impact of decreasing pH on the % of larvae reaching the pluteus stage leading to a positive effect (faster growth compared to pH 8.2) of low pH at 20 °C, a neutral effect at 24 °C and a negative effect (slower growth) at 26 °C. These results highlight the importance of considering a range of temperatures covering today and the future environmental variability in any experiment aiming at studying the impact of ocean acidification. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
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