1. PD-1 Expression in Head and Neck Squamous Cell Carcinomas Derives Primarily from Functionally Anergic CD4
- Author
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Austin K, Mattox, Jina, Lee, William H, Westra, Robert H, Pierce, Ronald, Ghossein, William C, Faquin, Thomas J, Diefenbach, Luc G, Morris, Derrick T, Lin, Lori J, Wirth, Armida, Lefranc-Torres, Eiichi, Ishida, Patrick D, Chakravarty, Lauren, Johnson, Yang C, Zeng, Huabiao, Chen, Mark C, Poznansky, Neil M, Iyengar, and Sara I, Pai
- Subjects
CD4-Positive T-Lymphocytes ,Clonal Anergy ,Macrophages ,Programmed Cell Death 1 Receptor ,hemic and immune systems ,chemical and pharmacologic phenomena ,Kaplan-Meier Estimate ,CD8-Positive T-Lymphocytes ,Immunohistochemistry ,B7-H1 Antigen ,Article ,Lymphocytes, Tumor-Infiltrating ,Microscopy, Fluorescence ,Carcinoma, Squamous Cell ,Tumor Microenvironment ,Humans ,Mouth Neoplasms - Abstract
Oral tongue squamous cell carcinoma (OTSCC) is the most common oral cavity tumor. In this study, we examined the basis for the activity of PD-1-based immune checkpoint therapy that is being explored widely in head and neck cancers. Using multispectral imaging, we systematically investigated the OTSCC tumor microenvironment (TME) by evaluating the frequency of PD-1 expression in CD8+, CD4+ and FoxP3+ tumor-infiltrating lymphocytes (TIL). We also defined the cellular sources of PD-L1 to evaluate the utility of PD-1:PD-L1 blocking antibody therapy in this patient population. PD-L1 was expressed in 79% of the OTSCC specimens examined within the TME. Expression of PD-L1 was associated with moderate to high levels of CD4+ and CD8+ TIL. We found that CD4+ TIL were present in equal or greater frequencies than CD8+ TIL in 94% of OTSCC, and that CD4+ FOXP3neg TIL were co-localized with PD-1/PD-L1/CD68 more frequently than CD8+ TIL. However, both CD4+PD1+ and CD8+PD1+ TIL were anergic in the setting of PD-L1 expression. Overall, our results highlight the importance of CD4+ TIL as pivotal regulators of PD-L1 levels and in determining the responsiveness of OTSCC to PD1-based immune checkpoint therapy.
- Published
- 2016