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Your search keyword '"J.David Reilly"' showing total 2 results
Start Over Author "J.David Reilly" Topic cloning, molecular
2 results on '"J.David Reilly"'

1. Cosmid library of the turkey herpesvirus genome constructed from nanogram quantities of viral DNA associated with an excess of cellular DNA

Author

Robert F. Silva and J.David Reilly

Subjects
viruses, Genetic Vectors, Chick Embryo, Molecular cloning, Biology, Genome, law.invention, chemistry.chemical_compound, law, Virology, Animals, Genomic library, Cloning, Molecular, Cells, Cultured, Herpesviridae, Gene Library, Electrophoresis, Agar Gel, Fibroblasts, Cosmids, Molecular biology, Blotting, Southern, Restriction enzyme, chemistry, DNA, Viral, Cosmid, Recombinant DNA, Polymorphism, Restriction Fragment Length, DNA, In vitro recombination
Abstract

A protocol was designed for the rapid and efficient construction of cosmid libraries from cell-associated viral genomes available in very low quantities. Purification of viral DNA from cellular DNA was unnecessary. The vast excess of cellular DNA compensated for the limited amount of available viral DNA, enabling titration of the restriction endonuclease partial digest. A cosmid library of the turkey herpesvirus DNA genome was constructed from 1.5 micrograms of cellular DNA containing approximately 6 nanograms of viral DNA.

Published
1993
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2. A double-cos-site vector containing a multiple cloning site flanked by T7 and T3 RNA polymerase promoters

Author

Robert F. Silva and J.David Reilly

Subjects
Turkeys, Genetic Vectors, Restriction Mapping, Molecular cloning, Biology, Bacteriophage T7, Genetics, Multiple cloning site, Escherichia coli, Animals, Cloning, Molecular, Promoter Regions, Genetic, Herpesvirus 2, Gallid, Gene Library, pBluescript, Promoter, General Medicine, DNA-Directed RNA Polymerases, Cosmids, Molecular biology, Restriction site, genomic DNA, Cosmid Vector, Cosmid, bacteria, T-Phages
Abstract

A double-cos-site cosmid vector, c2XMCS, contains a multiple cloning site (MCS) with fifteen restriction sites flanked by phage T7 and T3 RNA polymerase promoters. The two cos sites in the cosmid vector enable the construction of cosmid libraries from unfractionated partial digests of genomic DNA. This simple construct combines the efficiency of a double-cos-site cosmid with the versatility provided by the MCS from pBluescript.

Published
1993

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