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1. Fluorescence-Detection Size-Exclusion Chromatography -- An Analytical Technique with Multiple Applications.

Author

Halan, Vivek, Maity, Sunit, Rathore, Anurag S., and Krull, Ira

Subjects
*GEL permeation chromatography, *CRYSTALLIZATION, *MEMBRANE proteins, *PROTEIN expression, *CLONING
Abstract

The article offers information on Fluorescence-Detection Size-Exclusion Chromatography (FSEC), which is predominantly used for precrystallization screening and membrane protein expression studies. Topics discussed include implications of FSEC in protein characterization, use of FSEC in studying the expression of monoclonal antibodies and antibody fragments and use of FSEC in screening various clones and checking the movement of the fluorescent-labeled gene of interest.

Published
2014

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