1. Degradation and osteogenic induction of a SrHPO4-coated Mg–Nd–Zn–Zr alloy intramedullary nail in a rat femoral shaft fracture model
- Author
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Chang Jiang, Jian Zhang, Hua Huang, Guangyin Yuan, Senbo An, Xiuhui Wang, Zuo-qin Yan, Zhe Wang, Yuan Tian, Jia Pei, Yuanwu Cao, Xiao Wang, Zhi-Ying Pang, Wang Xinyuan, and Jun-Ming Huang
- Subjects
Materials science ,Biocompatibility ,Biophysics ,chemistry.chemical_element ,Bioengineering ,02 engineering and technology ,Bone healing ,engineering.material ,Corrosion ,law.invention ,Biomaterials ,Intramedullary rod ,03 medical and health sciences ,Coating ,law ,medicine ,030304 developmental biology ,0303 health sciences ,Magnesium ,Femoral fracture ,021001 nanoscience & nanotechnology ,medicine.disease ,chemistry ,Mechanics of Materials ,Ceramics and Composites ,engineering ,Surface modification ,0210 nano-technology ,Biomedical engineering - Abstract
Magnesium and Mg-based alloys are promising biomaterials for orthopedic implants because of their degradability, osteogenic effects, and biocompatibility. However, the drawbacks of these materials include high hydrogen gas production, unexpected corrosion resistance, and insufficient mechanical strength duration. Surface modification can protect these biomaterials and induce osteogenesis. In this work, a SrHPO4 coating was developed for our patented biodegradable Mg-Nd-Zn-Zr alloy (abbr. JDBM) through a chemical deposition method. The coating was characterized by in vitro immersion, ion release, and cytotoxicity tests, which showed a slower corrosion behavior and excellent cell viability. RNA sequencing of MC3T3E1 cells treated with SrHPO4-coated JDBM ion release test extract showed increased Tlr4, followed by the activation of the downstream PI3K/Akt signaling pathway, causing proliferation and growth of pre-osteoblasts. An intramedullary nail (IMN) was implanted in a femoral fracture rat model. Mechanical test, radiological and histological analysis suggested that SrHPO4-coated JDBM has superior mechanical properties, induces more bone formation, and decreases the degradation rate compared with uncoated JDBM and the administration of TLR4 inhibitor attenuated the new bone formation for fracture healing. SrHPO4 is a promising coating for JDBM implants, particularly for long-bone fractures.
- Published
- 2020