1. A novel monoclonal antibody specific for cocaine.
- Author
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Nakayama H, Kenjyou N, and Shigetoh N
- Subjects
- Animals, Antibodies, Monoclonal biosynthesis, Antibodies, Monoclonal isolation & purification, Antibody Formation, Antibody Specificity, Enzyme-Linked Immunosorbent Assay, Female, Fluorescence, Mice, Mice, Inbred A, Sensitivity and Specificity, Antibodies, Monoclonal immunology, Cocaine immunology, Hybridomas immunology, Immunoconjugates immunology
- Abstract
Detection systems for the illegal drug cocaine need to have a high sensitivity and specificity for cocaine and to be relatively easy to use. In the current study, a monoclonal antibody (MAb) with a high specificity for cocaine was produced. Enzyme-linked immunosorbent assay and fluorescence quenching immunoassay were used to screen the hybridomas. The MAb S27Y (IgG1) was shown to be sensitive and specific for cocaine and quenched fluorescence. Thus, S27Y has the potential to be used in screening assays for the rapid and sensitive detection of cocaine.
- Published
- 2013
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