5 results on '"Isosporiasis diagnosis"'
Search Results
2. A review of Cystoisospora felis and C. rivolta-induced coccidiosis in cats.
- Author
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Dubey JP
- Subjects
- Animals, Animals, Wild parasitology, Antiprotozoal Agents therapeutic use, Cat Diseases parasitology, Cat Diseases transmission, Cats, Feces parasitology, Intestines parasitology, Isosporiasis diagnosis, Isosporiasis drug therapy, Isosporiasis parasitology, Life Cycle Stages, Oocysts physiology, Toxoplasma classification, Cat Diseases diagnosis, Cat Diseases drug therapy, Coccidiosis veterinary, Isospora classification, Isospora physiology, Isosporiasis veterinary
- Abstract
Until the discovery of Toxoplasma gondii oocysts in cat feces in 1970, little was known of coccidiosis in cats. Until 1970, three coccidian parasites based on different sized oocysts were recognized, the parasite with large oocysts (∼40 μm long and called Isospora felis), medium sized oocysts (∼25 μm long, called Isospora rivolta), and small sized oocysts (14 μm or less, called Isospora bigemina) were known and they were considered not host-specific. Later, it was demonstrated that these parasites were host-specific and had also extra-intestinal stages. The Isospora bigemina turned out to be more than 25 organisms belonging to T. gondii, Hammondia spp., Sarcocystis spp., Besnoitia spp., and Neospora spp.; these subjects have been reviewed previously in detail. The present paper summarizes biology of Isospora felis, and I. rivolta (now transferred to genus Cystoisospora), including taxonomy, life cycle, diagnosis, and treatment. Re-excretion of T. gondii oocysts from chronically infected cats after superinfection with Cystoisospora felis oocysts is discussed. There are only two species of Cystoisospora species in cats, C. felis and C. rivolta; Isospora novocati and Cystoisospora frenkeli named for I. rivolta-like parasites of cats are considered synonym of C. rivolta. Clinical coccidiosis occurs more commonly in recently weaned kittens and C. felis infections are more prevalent than C. rivolta., (Published by Elsevier B.V.)
- Published
- 2018
- Full Text
- View/download PDF
3. Case-control study of pathogens involved in piglet diarrhea.
- Author
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Ruiz VL, Bersano JG, Carvalho AF, Catroxo MH, Chiebao DP, Gregori F, Miyashiro S, Nassar AF, Oliveira TM, Ogata RA, Scarcelli EP, and Tonietti PO
- Subjects
- Animals, Animals, Newborn, Campylobacter isolation & purification, Campylobacter Infections diagnosis, Campylobacter Infections microbiology, Case-Control Studies, Coccidiosis diagnosis, Coccidiosis parasitology, Coinfection, Coronavirus isolation & purification, Coronavirus Infections diagnosis, Coronavirus Infections virology, Diarrhea microbiology, Diarrhea parasitology, Diarrhea virology, Eimeria isolation & purification, Feces microbiology, Feces parasitology, Feces virology, Isospora isolation & purification, Isosporiasis diagnosis, Isosporiasis parasitology, Rotavirus isolation & purification, Rotavirus Infections diagnosis, Rotavirus Infections virology, Strongylida isolation & purification, Strongylida Infections diagnosis, Strongylida Infections parasitology, Swine, Swine Diseases microbiology, Swine Diseases parasitology, Swine Diseases virology, Campylobacter Infections veterinary, Coccidiosis veterinary, Coronavirus Infections veterinary, Diarrhea veterinary, Isosporiasis veterinary, Rotavirus Infections veterinary, Strongylida Infections veterinary, Swine Diseases diagnosis
- Abstract
Background: Diarrhea in piglets directly affects commercial swine production. The disease results from the interaction of pathogens with the host immune system and is also affected by management procedures. Several pathogenic agents such as Campylobacter spp., Clostridium perfringens, Escherichia coli, Salmonella spp., group A rotavirus (RV-A), coronaviruses (transmissible gastroenteritis virus; porcine epidemic diarrhea virus), as well as nematode and protozoan parasites, can be associated with disease cases., Results: All bacterial, viral, protozoan, and parasitic agents here investigated, with the exception of Salmonella spp. as well as both coronaviruses, were detected in varying proportions in piglet fecal samples, and positive animals were equally distributed between case and control groups. A statistically significant difference between case and control groups was found only for Cystoisospora suis (p = 0.034) and Eimeria spp. (p = 0.047). When co-infections were evaluated, a statistically significant difference was found only for C. perfringens β2 and C. suis (p = 0.014)., Conclusions: The presence of pathogens in piglets alone does not determine the occurrence of diarrhea episodes. Thus, the indiscriminate use of antibiotic and anthelminthic medication should be re-evaluated. This study also reinforces the importance of laboratory diagnosis and correct interpretation of results as well as the relevance of control and prophylactic measures.
- Published
- 2016
- Full Text
- View/download PDF
4. Oocyst shedding by green-winged-saltator (Saltator similis) in the diagnostic of coccidiosis and Isospora similisi n. sp. (Apicomplexa: Eimeriidae).
- Author
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Coelho CD, Berto BP, Neves DM, Oliveira VM, Flausino W, and Lopes CW
- Subjects
- Animals, Coccidiosis diagnosis, Isosporiasis diagnosis, Bird Diseases diagnosis, Bird Diseases parasitology, Coccidiosis veterinary, Isosporiasis veterinary, Oocysts, Passeriformes parasitology
- Abstract
Diurnal periodicity is a phenomenon that has been observed in coccidian of Isospora parasites of passerines, which have been eliminated great number of oocysts at dusk. The objective of this study was to evaluate the occurrence of periodicity of oocysts presence in the green-winged-saltator Saltator similis, and its use in the diagnosis of coccidiosis in wild birds in captivity. A total of 220 fecal samples were collected from birds, apprehended from illegal trading and kept in quarantine in CETAS∕IBAMA, in the morning and late afternoon, from May to November 2010. It was observed that 1.82% of the samples collected in the morning were positive, while 31.36% of samples were positive in the late afternoon. In addition, the number of oocysts shed was greater in the afternoon. Therefore, it was concluded that the sampling in the late afternoon provided greater reliability for the diagnosis of coccidiosis in green-winged-saltators. Moreover, in this study a new isosporoid coccidian parasite from the green-winged-saltator S. similis was observed and is herein described. Isospora similisi n. sp. oocysts are spheroidal to sub-spheroidal, 27.5 × 25.9 µm, with a smooth and bi-layered wall, ∼1.2 mm. Micropyle and oocyst residuum are absent, but splinter-like or comma-like granules are present. Sporocysts are ellipsoidal or slightly ovoidal, 17.4 × 12.2 mm. A stieda body and substieda body are present. The sporocyst residuum is composed of granules of different sizes. Sporozoites are vermiform with a single refractile body and a nucleus. This is the fourth description of an isosporoid coccidium infecting S. similis and the sixth description from Cardinalidae.
- Published
- 2013
- Full Text
- View/download PDF
5. PCR-based differentiation of three porcine Eimeria species and Isospora suis.
- Author
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Ruttkowski B, Joachim A, and Daugschies A
- Subjects
- Animals, Coccidiosis diagnosis, Coccidiosis parasitology, DNA Primers, DNA, Protozoan chemistry, DNA, Protozoan genetics, DNA, Protozoan isolation & purification, Eimeria growth & development, Isospora growth & development, Isosporiasis diagnosis, Isosporiasis parasitology, Polymerase Chain Reaction methods, Species Specificity, Swine, Swine Diseases diagnosis, Coccidiosis veterinary, Isosporiasis veterinary, Polymerase Chain Reaction veterinary, Swine Diseases parasitology
- Abstract
Isospora suis and Eimeria are frequent coccidian parasites of pigs. The unsporulated oocysts of Eimeria species and of I. suis are difficult to differentiate. Therefore, a species-specific PCR was developed. PCR products were amplified from Eimeria polita, Eimeria porci, and Eimeria scabra using primers from the conserved 18S rRNA regions and were subsequently sequenced. Based on variable sequence regions, primers were constructed for the differentiation of the three Eimeria species and I. suis. Using a combination of PCRs detecting one or two species, all four coccidian species were detected (theoretical lower detection level: DNA content of 250 oocysts of each Eimeria species or 25 oocysts of Isospora in 1microl) and differentiated. The PCR-based differentiation of the above mentioned species provides a useful alternative to microscopy.
- Published
- 2001
- Full Text
- View/download PDF
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